Acute Lung Injury is Reduced in fat-1 Mice Endogenously Synthesizing n-3 Fatty Acids (2009)
Mayer, K., Kiessling, A., Ott, J., Schaefer, M.B., Hecker, M., Henneke, I., ...
RATIONALE: acute lung injury (ALI) remains an important cause of mortality in intensive care units. Inflammation is controlled by cytokines and eicosanoids derived from the n-6 fatty acid (FA)...
Zhu L, M. Hecker, J. Atesang, Ritz Y, H. Geisler, U. Herr, ...
Experimental challenges for approaching local strain
Schaefer, M.B., Pose, A., Ott, J., Hecker, M., Behnk, A., Schulz, R., ...
Acute lung injury (ALI) still represents a major cause of morbidity and mortality in intensive care units. Beneficial effects have been described after activation of the peroxisome...
pSM19035-encoded zeta toxin induces stasis followed by death in a subpopulation of cells. (2006)
Lioy, V.S., Martin, M.T., Camaccho, A.G., Lurz, R., Antelmann, H., Hecker, M., ...
pSM19035-encoded zeta toxin induces stasis followed by death in a subpopulation of cells. (2006)
Lioy, V.S., Martin, M.T., Camaccho, A.G., Lurz, R., Antelmann, H., Hecker, M., ...
Genotype-dependent NOS-3 expression and rheumatoid arthritis (2005)
Cattaruzza, M, Melchers, I, Hecker, M
No abstract available.
Schumann, J., Kleint, C.A., Vinzelberg, H., Thomas, J., Hecker, M., Nurnus, J., ...
Strain-symmetrized superlattice structures based on epitaxially grown Si/Ge multilayers are expected to be promising systems for efficient thermoelectric micro-systems. The paper presents the...
Influence of SiH4 on the WN4-PECVD process (2003)
Ecke, R., Schulz, S.E., Hecker, M., Mattern, N., Gessner, T.
Hecker, M, Boese, M, Schini-Kerth, V B, Mülsch, A, Busse, R
The nature of an L-arginine-derived relaxing factor released from vascular smooth muscle cells cultured on microcarrier beads and stimulated for 20 h with interleukin 1 beta was investigated. Unlike...
The metabolism of big endothelin 1 (bET) and endothelin 1 (ET-1) by subcellular fractions from human polymorphonuclear leukocytes (PMNs) was investigated by bioassay and reversed-phase...
Osteoclastic inhibition: an action of nitric oxide not mediated by cyclic GMP.
MacIntyre, I, Zaidi, M, Alam, A S, Datta, H K, Moonga, B S, Lidbury, P S, ...
The osteoclast is unique in its ability to resorb bone, and excessive osteoclastic activity has been implicated in osteoporosis, Paget disease of bone, rheumatoid arthritis, and the growth of...
Zembowicz, A, Hecker, M, Macarthur, H, Sessa, W C, Vane, J R
The hypothesis was investigated that NG-hydroxy-L-arginine (L-HOArg) is an intermediate in the biosynthesis of nitric oxide (.NO) from L-arginine (L-Arg) by the constitutive .NO synthase (NOS)...
Sessa, W C, Hecker, M, Mitchell, J A, Vane, J R
The mechanism by which L-glutamine (L-Gln) inhibits the release of endothelium-derived relaxing factor from bovine aortic cultured endothelial cells was investigated. The intracellular concentration...
Hecker, M, Sessa, W C, Harris, H J, Anggård, E E, Vane, J R
We have investigated the mechanism by which cultured endothelial cells generate L-arginine (L-Arg), the substrate for the biosynthesis of endothelium-derived relaxing factor. When Arg-depleted...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Antelmann, H, Bernhardt, J, Schmid, R, Hecker, M
In Bacillus subtilis, general stress proteins (Gsps) are induced in response to different stresses (heat, salt, or ethanol) or after nutrient starvation. The majority of the genes for the Gsps are...
Voelker, U, Voelker, A, Maul, B, Hecker, M, Dufour, A, Haldenwang, W G
sigma B is a secondary sigma factor that controls the general stress response of Bacillus subtilis. sigma B-dependent transcription is induced by the activation of sigma B itself, a process that...
Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis.
The expression of the putative operon bglPH of Bacillus subtilis was studied by using bglP'-lacZ transcriptional fusions. The bglP gene encodes an aryl-beta-glucoside-specific enzyme II of the...
Engelmann, S, Lindner, C, Hecker, M
A sigma B-dependent stress gene of Bacillus subtilis was localized downstream of the licS gene. The predicted amino acid sequence exhibited a significant similarity to the sequence of the...
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family.
Schnetz, K, Stülke, J, Gertz, S, Krüger, S, Krieg, M, Hecker, M, ...
Gene licS of Bacillus subtilis encodes an excreted Beta-1,3-1,4-endoglucanase necessary for lichenan utilization. Upstream of licS we found a gene (termed licT) together with its promoter which...
Krüger, S, Gertz, S, Hecker, M
In Bacillus subtilis, aryl-beta-glucosides such as salicin and arbutin are catabolized by the gene products of bglP and bglH, encoding an enzyme II of the phosphoenolpyruvate sugar-phosphotransferase...
Antelmann, H, Engelmann, S, Schmid, R, Hecker, M
The AhpC subunit of the Bacillus subtilis alkyl hydroperoxide reductase was identified as a general stress protein induced in response to heat or salt stress or after entry of the organism into the...
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis.
Tobisch, S, Glaser, P, Krüger, S, Hecker, M
A new catabolic system in Bacillus subtilis involved in utilization of beta-glucosidic compounds has been investigated. It consists of five genes encoding phosphotransferase system (PTS) enzyme II...
Antelmann, H, Engelmann, S, Schmid, R, Sorokin, A, Lapidus, A, Hecker, M
SigmaB-dependent general stress proteins (Gsps) of Bacillus subtilis are essential for the development of glucose-starvation-induced cross-resistance to oxidative challenge. However, the proteins...
Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.
Riethdorf, S, Völker, U, Gerth, U, Winkler, A, Engelmann, S, Hecker, M
The lon gene of Escherichia coli encodes the ATP-dependent serine protease La and belongs to the family of sigma 32-dependent heat shock genes. In this paper, we report the cloning and...
Stress induction of clpC in Bacillus subtilis and its involvement in stress tolerance.
Krüger, E, Völker, U, Hecker, M
A member of the clpC subfamily of stress response-related Clp ATPases was cloned from Bacillus subtilis. The B. subtilis clpC gene was induced in response to various stresses, including heat shock....
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
Wetzstein, M, Völker, U, Dedio, J, Löbau, S, Zuber, U, Schiesswohl, M, ...
By using an internal part of the dnaK gene from Bacillus megaterium as a probe, a 5.2-kb HindIII fragment of chromosomal DNA of Bacillus subtilis was cloned. Downstream sequences were isolated by in...
Schmidt, A, Schiesswohl, M, Völker, U, Hecker, M, Schumann, W
Using a gene probe of the Escherichia coli groEL gene, a 1.8-kb HindIII fragment of chromosomal DNA of Bacillus subtilis was cloned. Upstream sequences were isolated as a 3-kb PstI fragment....
Hecker, M, Mülsch, A, Bassenge, E, Förstermann, U, Busse, R
Endothelial cells (EC) contain a constitutive Ca2+/calmodulin-dependent nitric oxide (NO) synthase (cNOS) which plays an important role in the local control of vascular tone. We compared the...
Hecker, M, Bara, A T, Bauersachs, J, Busse, R
1. In addition to nitric oxide (NO) and prostacyclin (PGI2) an as yet unidentified endothelium-derived hyperpolarizing factor (EDHF) contributes to the dilator effect of bradykinin in different...
Popp, R, Bauersachs, J, Hecker, M, Fleming, I, Busse, R
1. The vascular endothelium releases a hyperpolarizing factor (endothelium-derived hyperpolarizing factor, EDHF) tentatively identified as a cytochrome P450-derived arachidonic acid metabolite....
Hecker, M, Boese, M, Schini-Kerth, V B, Mülsch, A, Busse, R
The nature of an L-arginine-derived relaxing factor released from vascular smooth muscle cells cultured on microcarrier beads and stimulated for 20 h with interleukin 1 beta was investigated. Unlike...
The metabolism of big endothelin 1 (bET) and endothelin 1 (ET-1) by subcellular fractions from human polymorphonuclear leukocytes (PMNs) was investigated by bioassay and reversed-phase...
Osteoclastic inhibition: an action of nitric oxide not mediated by cyclic GMP.
MacIntyre, I, Zaidi, M, Alam, A S, Datta, H K, Moonga, B S, Lidbury, P S, ...
The osteoclast is unique in its ability to resorb bone, and excessive osteoclastic activity has been implicated in osteoporosis, Paget disease of bone, rheumatoid arthritis, and the growth of...
Zembowicz, A, Hecker, M, Macarthur, H, Sessa, W C, Vane, J R
The hypothesis was investigated that NG-hydroxy-L-arginine (L-HOArg) is an intermediate in the biosynthesis of nitric oxide (.NO) from L-arginine (L-Arg) by the constitutive .NO synthase (NOS)...
Sessa, W C, Hecker, M, Mitchell, J A, Vane, J R
The mechanism by which L-glutamine (L-Gln) inhibits the release of endothelium-derived relaxing factor from bovine aortic cultured endothelial cells was investigated. The intracellular concentration...
Hecker, M, Sessa, W C, Harris, H J, Anggård, E E, Vane, J R
We have investigated the mechanism by which cultured endothelial cells generate L-arginine (L-Arg), the substrate for the biosynthesis of endothelium-derived relaxing factor. When Arg-depleted...
Essential Bacillus subtilis genes
Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., ...
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among ≈4,100 genes of the...
Antelmann, H, Bernhardt, J, Schmid, R, Hecker, M
In Bacillus subtilis, general stress proteins (Gsps) are induced in response to different stresses (heat, salt, or ethanol) or after nutrient starvation. The majority of the genes for the Gsps are...
Voelker, U, Voelker, A, Maul, B, Hecker, M, Dufour, A, Haldenwang, W G
sigma B is a secondary sigma factor that controls the general stress response of Bacillus subtilis. sigma B-dependent transcription is induced by the activation of sigma B itself, a process that...
Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis.
The expression of the putative operon bglPH of Bacillus subtilis was studied by using bglP'-lacZ transcriptional fusions. The bglP gene encodes an aryl-beta-glucoside-specific enzyme II of the...
Engelmann, S, Lindner, C, Hecker, M
A sigma B-dependent stress gene of Bacillus subtilis was localized downstream of the licS gene. The predicted amino acid sequence exhibited a significant similarity to the sequence of the...
LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family.
Schnetz, K, Stülke, J, Gertz, S, Krüger, S, Krieg, M, Hecker, M, ...
Gene licS of Bacillus subtilis encodes an excreted Beta-1,3-1,4-endoglucanase necessary for lichenan utilization. Upstream of licS we found a gene (termed licT) together with its promoter which...
Krüger, S, Gertz, S, Hecker, M
In Bacillus subtilis, aryl-beta-glucosides such as salicin and arbutin are catabolized by the gene products of bglP and bglH, encoding an enzyme II of the phosphoenolpyruvate sugar-phosphotransferase...
Antelmann, H, Engelmann, S, Schmid, R, Hecker, M
The AhpC subunit of the Bacillus subtilis alkyl hydroperoxide reductase was identified as a general stress protein induced in response to heat or salt stress or after entry of the organism into the...
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis.
Tobisch, S, Glaser, P, Krüger, S, Hecker, M
A new catabolic system in Bacillus subtilis involved in utilization of beta-glucosidic compounds has been investigated. It consists of five genes encoding phosphotransferase system (PTS) enzyme II...
Antelmann, H, Engelmann, S, Schmid, R, Sorokin, A, Lapidus, A, Hecker, M
SigmaB-dependent general stress proteins (Gsps) of Bacillus subtilis are essential for the development of glucose-starvation-induced cross-resistance to oxidative challenge. However, the proteins...
Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.
Riethdorf, S, Völker, U, Gerth, U, Winkler, A, Engelmann, S, Hecker, M
The lon gene of Escherichia coli encodes the ATP-dependent serine protease La and belongs to the family of sigma 32-dependent heat shock genes. In this paper, we report the cloning and...
Stress induction of clpC in Bacillus subtilis and its involvement in stress tolerance.
Krüger, E, Völker, U, Hecker, M
A member of the clpC subfamily of stress response-related Clp ATPases was cloned from Bacillus subtilis. The B. subtilis clpC gene was induced in response to various stresses, including heat shock....
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
Wetzstein, M, Völker, U, Dedio, J, Löbau, S, Zuber, U, Schiesswohl, M, ...
By using an internal part of the dnaK gene from Bacillus megaterium as a probe, a 5.2-kb HindIII fragment of chromosomal DNA of Bacillus subtilis was cloned. Downstream sequences were isolated by in...
Schmidt, A, Schiesswohl, M, Völker, U, Hecker, M, Schumann, W
Using a gene probe of the Escherichia coli groEL gene, a 1.8-kb HindIII fragment of chromosomal DNA of Bacillus subtilis was cloned. Upstream sequences were isolated as a 3-kb PstI fragment....
Hecker, M, Mülsch, A, Bassenge, E, Förstermann, U, Busse, R
Endothelial cells (EC) contain a constitutive Ca2+/calmodulin-dependent nitric oxide (NO) synthase (cNOS) which plays an important role in the local control of vascular tone. We compared the...
Hecker, M, Bara, A T, Bauersachs, J, Busse, R
1. In addition to nitric oxide (NO) and prostacyclin (PGI2) an as yet unidentified endothelium-derived hyperpolarizing factor (EDHF) contributes to the dilator effect of bradykinin in different...
Popp, R, Bauersachs, J, Hecker, M, Fleming, I, Busse, R
1. The vascular endothelium releases a hyperpolarizing factor (endothelium-derived hyperpolarizing factor, EDHF) tentatively identified as a cytochrome P450-derived arachidonic acid metabolite....
Wigand, R., Meyer, J., Busse, R., Hecker, M.
OBJECTIVES—To determine the feasibility of monitoring the serum concentration of NG-hydroxy-L-arginine (L-NHA) as an index of an increased nitric oxide (NO) synthase activity in patients with...
Mapping the Pathways to Staphylococcal Pathogenesis by Comparative Secretomics
Ziebandt, A. K., Engelmann, S., Hecker, M., De Jong, A., Harmsen, H. J. M., ...
The gram-positive bacterium Staphylococcus aureus is a frequent component of the human microbial flora that can turn into a dangerous pathogen. As such, this organism is capable of infecting almost...
Bauersachs, J, Hecker, M, Busse, R
1. In addition to nitric oxide (NO) and prostacyclin (PGI2) an endothelium-derived factor, which hyperpolarizes vascular smooth muscle cells via activation of K+ channels, contributes to the dilator...
CD40 antisense oligonucleotide inhibition of trinitrobenzene sulphonic acid induced rat colitis
Gao, D, Wagner, A H, Fankhaenel, S, Stojanovic, T, Schweyer, S, Panzner, S, ...
Background: CD154/CD40 interactions play a pivotal role both in humoral and cellular immune responses. Their involvement in the pathogenesis of chronic inflammatory bowel disease (IBD) has been...
Macarthur, H., Hecker, M., Busse, R., Vane, J. R.
1. The effects of the Ca(2+)-ATPase inhibitor, thapsigargin, on the shear stress-dependent and on the agonist-stimulated release of endothelium-derived relaxing factor, i.e. nitric oxide (NO), and...
Zembowicz, A., Swierkosz, T. A., Southan, G. J., Hecker, M., Vane, J. R.
1. We recently demonstrated that NG-hydroxy-L-arginine (L-HOArg) is a substrate for the constitutive nitric oxide (NO) synthase present in bovine aortic endothelial cells cultured on microcarrier...
Thiemermann, C., Mustafa, M., Mester, P. A., Mitchell, J. A., Hecker, M., Vane, J. R.
1. We have shown that dipeptides containing NG-nitro-L-arginine (NO2Arg) inhibit the biosynthesis of endothelium-derived relaxing factor (EDRF) in vitro and in vivo. 2. In anaesthetized rats,...
Klemm, P., Hecker, M., Stockhausen, H., Wu, C. C., Thiemermann, C.
1. Induction of the calcium-independent isoform of nitric oxide (NO) synthase (iNOS) in various cell types has been implicated in the circulatory failure in experimental models of septic shock....
Lischke, V., Busse, R., Hecker, M.
1. Several lines of evidence suggest that both volatile and intravenous anaesthetics may interfere with the synthesis and release of endothelium-derived vasoactive factors. We have investigated the...
Hecker, M., Preiss, C., Klemm, P., Busse, R.
1. In view of the potential deleterious effects of high amounts of nitric oxide (NO) produced by the inducible isoform of NO synthase (iNOS) in inflammation, the prevention of the expression of this...
Hecker, M., Pörsti, I., Bara, A. T., Busse, R.
1. To examine the possibility that angiotensin-converting enzyme (ACE) inhibitors modulate the action of bradykinin at the receptor level, their effect on the dilator response to bradykinin was...
Pörsti, I., Bara, A. T., Busse, R., Hecker, M.
The angiotensin I (AI) metabolite, A(1-7), elicited a concentration-dependent dilator response (ED50 > or = 2 microM) in porcine coronary artery rings which was markedly attenuated by the nitric...
Hecker, M., Mitchell, J. A., Swierkosz, T. A., Sessa, W. C., Vane, J. R.
L-Glutamine (0.02-2 mM) but not D-glutamine (0.2 mM and 2 mM) inhibited the release of endothelium-derived relaxing factor (EDRF) from bovine aortic endothelial cells cultured in the presence or...