Moroianu, J, Hijikata, M, Blobel, G, Radu, A
Although only 44% identical to human karyopherin alpha 1, human karyopherin alpha 2 (Rch1 protein) substituted for human karyopherin alpha 1 (hSRP-1/NPI-1) in recognizing a standard nuclear...
Hijikata, M, Mizushima, H, Tanji, Y, Komoda, Y, Hirowatari, Y, Akagi, T, ...
By using a plasmid-based transient protein expression system in cultured cells and an in vitro transcription/translation system, we analyzed the proteolytic processing of the putative nonstructural...
Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line.
Shimizu, Y K, Iwamoto, A, Hijikata, M, Purcell, R H, Yoshikura, H
A human T-cell line, MOLT-4, either uninfected or infected with murine retroviruses, was tested for its susceptibility to hepatitis C virus (HCV) infection. The cell cultures were inoculated with a...
Hijikata, M, Kato, N, Ootsuyama, Y, Nakagawa, M, Shimotohno, K
Processing of the putative structural proteins of hepatitis C virus was examined by using an in vitro expression system. An RNA transcript for cell-free translation was prepared from a cDNA construct...
Kato, N, Hijikata, M, Ootsuyama, Y, Nakagawa, M, Ohkoshi, S, Sugimura, T, ...
The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese...
Tanji, Y, Hijikata, M, Satoh, S, Kaneko, T, Shimotohno, K
A transient protein expression system in COS-1 cells was used to study the role of hepatitis C virus (HCV)-encoded NS4A protein on HCV nonstructural polyprotein processing. By analyzing the protein...
Satoh, S, Tanji, Y, Hijikata, M, Kimura, K, Shimotohno, K
Hepatitis C virus proteins are produced by proteolytic processing of the viral precursor polyprotein that is encoded in the largest open reading frame of the viral genome. Processing of the...
Characterization of long-term cultures of hepatitis C virus.
Nakajima, N, Hijikata, M, Yoshikura, H, Shimizu, Y K
The human T- and B-cell lines HPBMa10-2 and Daudi produced infectious hepatitis C virus (HCV) for more than 1 year after infection. The infectivity titer of the cell culture-grown HCV and its genome...
Tsuchihara, K, Tanaka, T, Hijikata, M, Kuge, S, Toyoda, H, Nomoto, A, ...
We previously identified a highly conserved 98-nucleotide (nt) sequence, the 3'X, as the extreme 3'-terminal structure of the hepatitis C virus (HCV) genome (T. Tanaka, N. Kato, M.-J. Cho, and K....
Kato, N, Ootsuyama, Y, Sekiya, H, Ohkoshi, S, Nakazawa, T, Hijikata, M, ...
The hypervariable region 1 (HVR1) of the putative second envelope glycoprotein (gp70) of hepatitis C virus (HCV) contains a sequence-specific immunological B-cell epitope that induces the production...
Shimizu, Y K, Hijikata, M, Iwamoto, A, Alter, H J, Purcell, R H, Yoshikura, H
We developed an in vitro assay for antibodies to hepatitis C virus (HCV) that bind to virions and prevent initiation of the replication cycle in susceptible cells in vitro. These antibodies therefore...
Analysis of N-terminal processing of hepatitis C virus nonstructural protein 2.
Mizushima, H, Hijikata, M, Tanji, Y, Kimura, K, Shimotohno, K
We determined the partial amino (N)-terminal amino acid sequence of hepatitis C virus p21 (nonstructural protein 2 [NS2]). Cleavage at the p21 (NS2) N terminus depended on the presence of microsomal...
Two hepatitis C virus glycoprotein E2 products with different C termini.
Mizushima, H, Hijikata, M, Asabe, S, Hirota, M, Kimura, K, Shimotohno, K
Processing of the boundary region between the putative structural and nonstructural regions of the hepatitis C virus precursor polyprotein was analyzed by in vitro translation using reticulocyte...
Komoda, Y, Hijikata, M, Sato, S, Asabe, S, Kimura, K, Shimotohno, K
Using as substrates a series of chimeric proteins containing various fragments of the hepatitis C virus precursor polyprotein between Escherichia coli maltose binding protein and dihydrofolate...
Tanji, Y, Hijikata, M, Hirowatari, Y, Shimotohno, K
Hepatitis C virus (HCV) serine proteinase (Cpro-2) is responsible for the processing of HCV nonstructural (NS) protein processing. To clarify the mechanism of Cpro-2-dependent processing, pulse-chase...
Kato, N, Sekiya, H, Ootsuyama, Y, Nakazawa, T, Hijikata, M, Ohkoshi, S, ...
We recently found that alterations of amino acids in hypervariable region 1 (HVR1) of the putative envelope glycoprotein (gp70) of hepatitis C virus (HCV) occurred sequentially in the chronic phase...
Hijikata, M, Mizushima, H, Akagi, T, Mori, S, Kakiuchi, N, Kato, N, ...
Gene products of hepatitis C virus (HCV), a possible major causative agent of posttransfusion non-A, non-B hepatitis, are considered to be produced from a precursor polyprotein via proteolytic...
Equilibrium centrifugation studies of hepatitis C virus: evidence for circulating immune complexes.
Hijikata, M, Shimizu, Y K, Kato, H, Iwamoto, A, Shih, J W, Alter, H J, ...
The buoyant density of hepatitis C virus (HCV), with high in vivo infectivity (strain H) or low in vivo infectivity (strain F), was determined by sucrose gradient equilibrium centrifugation. Viral...
Hijikata, M, Kato, N, Sato, T, Kagami, Y, Shimotohno, K
To identify gene products that might be involved in leukemogenesis of adult T-cell leukemia (ATL), we constructed a cDNA library from an ATL tumor cell line named IKD. By differential plaque...
Complete nucleotide sequence of cDNA and deduced amino acid sequence of rat liver catalase.
Furuta, S, Hayashi, H, Hijikata, M, Miyazawa, S, Osumi, T, Hashimoto, T
We have isolated five cDNA clones for rat liver catalase (hydrogen peroxide:hydrogen peroxide oxidoreductase, EC 1.11.1.6). These clones overlapped with each other and covered the entire length of...
Caspase-mediated cleavage of eukaryotic translation initiation factor subunit 2alpha.
Satoh, S, Hijikata, M, Handa, H, Shimotohno, K
Eukaryotic translation initiation factor 2alpha (eIF-2alpha), a target molecule of the interferon-inducible double-stranded-RNA-dependent protein kinase (PKR), was cleaved in apoptotic Saos-2 cells...
Transcriptional activities of p73 splicing variants are regulated by inter-variant association.
Ueda, Y, Hijikata, M, Takagi, S, Chiba, T, Shimotohno, K
p73 has been identified as a gene that encodes a protein with significant identity with the tumour suppressor p53. The main structural difference between p73 and p53 is the additional C-terminal...
Effect of Interaction between Hepatitis C Virus NS5A and NS5B on the Hepatitis C Virus Replicon
Shimakami, T., Hijikata, M., Luo, H., Ma, Y., Kanako, S., Shimotohno, K., ...
Moroianu, J, Hijikata, M, Blobel, G, Radu, A
Although only 44% identical to human karyopherin alpha 1, human karyopherin alpha 2 (Rch1 protein) substituted for human karyopherin alpha 1 (hSRP-1/NPI-1) in recognizing a standard nuclear...
Hijikata, M, Mizushima, H, Tanji, Y, Komoda, Y, Hirowatari, Y, Akagi, T, ...
By using a plasmid-based transient protein expression system in cultured cells and an in vitro transcription/translation system, we analyzed the proteolytic processing of the putative nonstructural...
Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line.
Shimizu, Y K, Iwamoto, A, Hijikata, M, Purcell, R H, Yoshikura, H
A human T-cell line, MOLT-4, either uninfected or infected with murine retroviruses, was tested for its susceptibility to hepatitis C virus (HCV) infection. The cell cultures were inoculated with a...
Hijikata, M, Kato, N, Ootsuyama, Y, Nakagawa, M, Shimotohno, K
Processing of the putative structural proteins of hepatitis C virus was examined by using an in vitro expression system. An RNA transcript for cell-free translation was prepared from a cDNA construct...
Kato, N, Hijikata, M, Ootsuyama, Y, Nakagawa, M, Ohkoshi, S, Sugimura, T, ...
The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese...
Tanji, Y, Hijikata, M, Satoh, S, Kaneko, T, Shimotohno, K
A transient protein expression system in COS-1 cells was used to study the role of hepatitis C virus (HCV)-encoded NS4A protein on HCV nonstructural polyprotein processing. By analyzing the protein...
Satoh, S, Tanji, Y, Hijikata, M, Kimura, K, Shimotohno, K
Hepatitis C virus proteins are produced by proteolytic processing of the viral precursor polyprotein that is encoded in the largest open reading frame of the viral genome. Processing of the...
Characterization of long-term cultures of hepatitis C virus.
Nakajima, N, Hijikata, M, Yoshikura, H, Shimizu, Y K
The human T- and B-cell lines HPBMa10-2 and Daudi produced infectious hepatitis C virus (HCV) for more than 1 year after infection. The infectivity titer of the cell culture-grown HCV and its genome...
Tsuchihara, K, Tanaka, T, Hijikata, M, Kuge, S, Toyoda, H, Nomoto, A, ...
We previously identified a highly conserved 98-nucleotide (nt) sequence, the 3'X, as the extreme 3'-terminal structure of the hepatitis C virus (HCV) genome (T. Tanaka, N. Kato, M.-J. Cho, and K....
Kato, N, Ootsuyama, Y, Sekiya, H, Ohkoshi, S, Nakazawa, T, Hijikata, M, ...
The hypervariable region 1 (HVR1) of the putative second envelope glycoprotein (gp70) of hepatitis C virus (HCV) contains a sequence-specific immunological B-cell epitope that induces the production...
Shimizu, Y K, Hijikata, M, Iwamoto, A, Alter, H J, Purcell, R H, Yoshikura, H
We developed an in vitro assay for antibodies to hepatitis C virus (HCV) that bind to virions and prevent initiation of the replication cycle in susceptible cells in vitro. These antibodies therefore...
Analysis of N-terminal processing of hepatitis C virus nonstructural protein 2.
Mizushima, H, Hijikata, M, Tanji, Y, Kimura, K, Shimotohno, K
We determined the partial amino (N)-terminal amino acid sequence of hepatitis C virus p21 (nonstructural protein 2 [NS2]). Cleavage at the p21 (NS2) N terminus depended on the presence of microsomal...
Two hepatitis C virus glycoprotein E2 products with different C termini.
Mizushima, H, Hijikata, M, Asabe, S, Hirota, M, Kimura, K, Shimotohno, K
Processing of the boundary region between the putative structural and nonstructural regions of the hepatitis C virus precursor polyprotein was analyzed by in vitro translation using reticulocyte...
Komoda, Y, Hijikata, M, Sato, S, Asabe, S, Kimura, K, Shimotohno, K
Using as substrates a series of chimeric proteins containing various fragments of the hepatitis C virus precursor polyprotein between Escherichia coli maltose binding protein and dihydrofolate...
Tanji, Y, Hijikata, M, Hirowatari, Y, Shimotohno, K
Hepatitis C virus (HCV) serine proteinase (Cpro-2) is responsible for the processing of HCV nonstructural (NS) protein processing. To clarify the mechanism of Cpro-2-dependent processing, pulse-chase...
Kato, N, Sekiya, H, Ootsuyama, Y, Nakazawa, T, Hijikata, M, Ohkoshi, S, ...
We recently found that alterations of amino acids in hypervariable region 1 (HVR1) of the putative envelope glycoprotein (gp70) of hepatitis C virus (HCV) occurred sequentially in the chronic phase...
Hijikata, M, Mizushima, H, Akagi, T, Mori, S, Kakiuchi, N, Kato, N, ...
Gene products of hepatitis C virus (HCV), a possible major causative agent of posttransfusion non-A, non-B hepatitis, are considered to be produced from a precursor polyprotein via proteolytic...
Equilibrium centrifugation studies of hepatitis C virus: evidence for circulating immune complexes.
Hijikata, M, Shimizu, Y K, Kato, H, Iwamoto, A, Shih, J W, Alter, H J, ...
The buoyant density of hepatitis C virus (HCV), with high in vivo infectivity (strain H) or low in vivo infectivity (strain F), was determined by sucrose gradient equilibrium centrifugation. Viral...
Hijikata, M, Kato, N, Sato, T, Kagami, Y, Shimotohno, K
To identify gene products that might be involved in leukemogenesis of adult T-cell leukemia (ATL), we constructed a cDNA library from an ATL tumor cell line named IKD. By differential plaque...
Complete nucleotide sequence of cDNA and deduced amino acid sequence of rat liver catalase.
Furuta, S, Hayashi, H, Hijikata, M, Miyazawa, S, Osumi, T, Hashimoto, T
We have isolated five cDNA clones for rat liver catalase (hydrogen peroxide:hydrogen peroxide oxidoreductase, EC 1.11.1.6). These clones overlapped with each other and covered the entire length of...
Caspase-mediated cleavage of eukaryotic translation initiation factor subunit 2alpha.
Satoh, S, Hijikata, M, Handa, H, Shimotohno, K
Eukaryotic translation initiation factor 2alpha (eIF-2alpha), a target molecule of the interferon-inducible double-stranded-RNA-dependent protein kinase (PKR), was cleaved in apoptotic Saos-2 cells...
Transcriptional activities of p73 splicing variants are regulated by inter-variant association.
Ueda, Y, Hijikata, M, Takagi, S, Chiba, T, Shimotohno, K
p73 has been identified as a gene that encodes a protein with significant identity with the tumour suppressor p53. The main structural difference between p73 and p53 is the additional C-terminal...