Biggs, M.J.P., Richards, R.G., Gadegaard, N., Wilkinson, C.D.W., Oreffo, R.O.C., Dalby, M.J.
The physiochemical characteristics of a material with in vivo applications are critical for the clinical success of the implant and regulate both cellular adhesion and differentiated cellular...
Rossi, L., Riehle, M.O., McNamara, L.E., Burchmore, R.J.S., Dalby, M.J., McMurray, R.J., ...
Abstract not available
Kantawong, F., Burchmore, R.J.S., Wilkinson, C.D.W., Oreffo, R.O.C., Dalby, M.J.
We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein...
Nanostructured surfaces: cell engineering and cell biology (2009)
No abstract available.
Kantawong, F., Burgess, K.E.V., Jayawardena, K., Hart, A., Burchmore, R.J., Gadegaard, N., ...
Topographic features can modulate cell behaviours such as proliferation, migration, differentiation and apoptosis. Biochemical mechanotransduction implies the conversion of mechanical forces (e.g....
Fluorescence two-dimensional difference gel electrophoresis for biomaterial applications (2009)
McNamara, L.E., Dalby, M.J., Riehle, M.O., Burchmore, R.J.S.
Fluorescence two-dimensional difference gel electrophoresis (DiGE) is rapidly becoming established as a powerful technique for the characterization of differences in protein expression levels between...
Sjostrom, T., Dalby, M.J., Hart, A., Tare, R., Oreffo, R.O.C., Su, B.
Surface nanotopography is known to influence the interaction of human skeletal (mesenchymal) stem cells (hMSC) with a material 14 surface. While most surface nanopatterning has been performed on...
Proteomic analysis of human osteoprogenitor response to disordered nanotopography (2008)
Kantawong, F., Burchmore, R.J.S., Gadegaard, N., Oreffo, R.O.C., Dalby, M.J.
Previous studies have shown that microgroove-initiated contact guidance can induce bone formation in osteoprogenitor cells (OPGs) and produce changes in the cell proteome. For proteomic analysis,...
Biggs, M.J.P., Richards, R.G., McFarlane, S., Wilkinson, C.D.W., Oreffo, R.O.C., Dalby, M.J.
The surface microtexture of an orthopaedic device can regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have...
Dalby, M.J., McNamara, L.E., Riehle, M.O., Burchmore, R.J.S., Kuntanawat, P., McMurray, R.J., ...
No abstract available.
Dalby, M.J., Hart, A., Yarwood, S.J.
Abstract: A wide variety of different cell types have been shown to respond to nanofabricated growth surfaces via the process of contact guidance, however little is known about the intracellular...
Biggs, M.J.P., Richards, R.G., McFarlane, S., Wilkinson, C.D.W., Oreffo, R.O.C., Dalby, M.J.
The surface microtexture of an orthopaedic device can regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have...
Dalby, M.J., Gadegaard, N., Wilkinson, C.D.W.
It has been known for many years that cells will react to the shape of their microenvironment. It is more recently becoming clear that cells can alter their morphology, adhesions, and cytoskeleton in...
Genomic expression of mesenchymal stem cells to altered nanoscale topographies (2008)
Dalby, M.J., Andar, A., Nag, A., Affrosman, S., Tare, R., McFarlane, S., ...
The understanding of cellular response to the shape of their environment would be of benefit in the development of artificial extracellular environments for potential use in the production of...
Biggs, M.J., Richards, R.G., Wilkinson, C.D., Dalby, M.J.
Current understanding of the mechanisms involved in osseointegration following implantation of a biomaterial has led to adhesion quantification being implemented as an assay of cytocompatibility....
Biggs, M.J.P., Richards, R.G., Gadegaard, N., McMurray, R.J., Affrosman, S., Wilkinson, C.D.W., ...
Polymeric medical devices widely used in orthopedic surgery play key roles in fracture fixation and orthopedic implant design. Topographical modification and surface micro-roughness of these devices...
Nanotopographical control of human osteoprogenitor differentiation (2007)
Dalby, M.J., Gadegaard, N., Curtis, A.S., Oreffo, R.O.
Current load-bearing orthopaedic implants are produced in 'bio-inert' materials such as titanium alloys. When inserted into the reamed bone during hip or knee replacement surgery the implants...
Osteoprogenitor response to semi-ordered and random nanotopographies (2006)
Dalby, M.J., McCloy, D., Robertson, M., Agheli, H.D., Affrossman, S., Oreffo, R.O.
In bone tissue engineering, it is desirable to use materials to control the differentiation of mesenchymal stem cell populations in order to gain direct bone apposition to implant materials. It has...
Osteoprogenitor response to defined topographies with nanoscale depths (2006)
Dalby, M.J., McCloy, D., Robertson, M., Wilkinson, C.D., Oreffo, R.O.
In the development of the next generation of orthopaedic implants for load-bearing applications, an ability to influence osteoprogenitor population activity and function will be highly desirable....
Berry, C.C., Dalby, M.J., Oreffo, R.O., McCloy, D., Affrosman, S.
Until now, nanotopography has been considered in 2D construct designs. This has been due to fabrication limitations with traditional lithographic processes relying on the ability to focus radiation...
Krishnamoorthy, S, Pugin, R, Liley, M, Dalby, M J, Oreffo, R O C, Heinzelmann, H, ...
This report will focus on creation of nanostructured PDMS surfaces with tunable feature dimensions and periodicities, and the use of these surfaces to study the influence of nanotopography on...
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
The environment around a cell during in vitro culture is unlikely to mimic those in vivo. Preliminary experiments with nanotopography have shown that nanoscale features can strongly influence cell...
Tubes with controllable internal nanotopography (2004)
Gadegaard, N., Dalby, M.J., Riehle, M.O., Curtis, A.S.G., Affrossman, S.
No abstract available.
Dalby, M.J., Gadegaard, N., Riehle, M.O., Wilkinson, C.D.W., Curtis, A.S.G.
In order for cells to react to topography, they must be able to sense shape. When considering nano-topography, these shapes are much smaller than the cell, but still strong responses to...
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
In designing new biomaterials, specific chemical and topographical cues will be important in guiding cell response. Filopodia are actin-driven structures produced by cells and speculated to be...
Dalby, M.J., Berry, C.C., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
Control of the cells' nanoenvironment is likely to be important in the future of cell and tissue engineering. Microtopography has been shown to provide cues to cells that elicit a large range of cell...
Cells react to nanoscale order and symmetry in their surroundings (2004)
Curtis, A.S.G., Gadegaard, N., Dalby, M.J., Riehle, M.O., Wilkinson, C.D.W., Aitchison, G.
Mammalian cells react to microstructured surfaces, but there is little information on the reactions to nanostructured surfaces, and such as have been tested are poorly ordered or random in their...
Fibroblast response to a controlled nanoenvironment produced by colloidal lithography (2004)
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
It is thought that by understanding how cells respond to topography, that better tissue engineering may be achievable. An important consideration in the cellular environment is topography. The...
Berry, C.C., Charles, S., Wells, S., Dalby, M.J., Curtis, A.S.G.
Magnetic nanoparticles have been used for bio-medical purposes including drug delivery, cell destruction and as MRI contrast agents for several years. A more recent biological application has focused...
Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography (2004)
Dalby, M.J., Giannaras, D., Riehle, M.O., Gadegaard, N., Affrossman, S., Curtis, A.S.G.
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical...
Use of nanotopography to study mechanotransduction in fibroblasts – methods and perspectives (2004)
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
The environment around a cell during in vitro culture is unlikely to mimic those in vivo. Preliminary experiments with nanotopography have shown that nanoscale features can strongly influence cell...
Dalby, M.J., Riehle, M.O., Johnstone, H., Affrossman, S., Curtis, A.S.G.
Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of...
Dalby, M.J., Riehle, M.O., Johnstone, H.J.H., Affrossman, S., Curtis, A.S.G.
It is becoming clear that cells do not only respond to micrometric scale topography, but may also respond to topography at the nanometric scale. Nano-fabrication methods such as electron beam...
Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography (2003)
Dalby, M.J., Riehle, M.O., Yarwood, S.J., Wilkinson, C.D.W., Curtis, A.S.G.
Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new...
Cells react to nanoscale order and symmetry in their surroundings
Curtis, A.S.G., Gadegaard, N., Dalby, M.J., Riehle, M.O., Wilkinson, C.D.W., Aitchison, G.
Mammalian cells react to microstructured surfaces, but there is little information on the reactions to nanostructured surfaces, and such as have been tested are poorly ordered or random in their...
Use of nanotopography to study mechanotransduction in fibroblasts – methods and perspectives
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
The environment around a cell during in vitro culture is unlikely to mimic those in vivo. Preliminary experiments with nanotopography have shown that nanoscale features can strongly influence cell...
Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography
Dalby, M.J., Riehle, M.O., Yarwood, S.J., Wilkinson, C.D.W., Curtis, A.S.G.
Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new...
Attempted endocytosis of nano-environment produced by colloidal lithography by human fibroblasts
Dalby, M.J., Berry, C.C., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
Control of the cells' nanoenvironment is likely to be important in the future of cell and tissue engineering. Microtopography has been shown to provide cues to cells that elicit a large range of cell...
Dalby, M.J., Riehle, M.O., Johnstone, H., Affrossman, S., Curtis, A.S.G.
Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of...
Tubes with controllable internal nanotopography
Gadegaard, N., Dalby, M.J., Riehle, M.O., Curtis, A.S.G., Affrossman, S.
No abstract available.
Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size
Dalby, M.J., Gadegaard, N., Riehle, M.O., Wilkinson, C.D.W., Curtis, A.S.G.
In order for cells to react to topography, they must be able to sense shape. When considering nano-topography, these shapes are much smaller than the cell, but still strong responses to...
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
The environment around a cell during in vitro culture is unlikely to mimic those in vivo. Preliminary experiments with nanotopography have shown that nanoscale features can strongly influence cell...
Berry, C.C., Charles, S., Wells, S., Dalby, M.J., Curtis, A.S.G.
Magnetic nanoparticles have been used for bio-medical purposes including drug delivery, cell destruction and as MRI contrast agents for several years. A more recent biological application has focused...
Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
In designing new biomaterials, specific chemical and topographical cues will be important in guiding cell response. Filopodia are actin-driven structures produced by cells and speculated to be...
Fibroblast response to a controlled nanoenvironment produced by colloidal lithography
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
It is thought that by understanding how cells respond to topography, that better tissue engineering may be achievable. An important consideration in the cellular environment is topography. The...
Dalby, M.J., Riehle, M.O., Johnstone, H.J.H., Affrossman, S., Curtis, A.S.G.
It is becoming clear that cells do not only respond to micrometric scale topography, but may also respond to topography at the nanometric scale. Nano-fabrication methods such as electron beam...
Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography
Dalby, M.J., Giannaras, D., Riehle, M.O., Gadegaard, N., Affrossman, S., Curtis, A.S.G.
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical...
Cells react to nanoscale order and symmetry in their surroundings
Curtis, A.S.G., Gadegaard, N., Dalby, M.J., Riehle, M.O., Wilkinson, C.D.W., Aitchison, G.
Mammalian cells react to microstructured surfaces, but there is little information on the reactions to nanostructured surfaces, and such as have been tested are poorly ordered or random in their...
Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography
Dalby, M.J., Riehle, M.O., Yarwood, S.J., Wilkinson, C.D.W., Curtis, A.S.G.
Cellular response to scaffold materials is of great importance in cellular and tissue engineering, and it is perhaps the initial cell contact with the scaffold that determines development of new...
Use of nanotopography to study mechanotransduction in fibroblasts – methods and perspectives
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
The environment around a cell during in vitro culture is unlikely to mimic those in vivo. Preliminary experiments with nanotopography have shown that nanoscale features can strongly influence cell...
Berry, C.C., Charles, S., Wells, S., Dalby, M.J., Curtis, A.S.G.
Magnetic nanoparticles have been used for bio-medical purposes including drug delivery, cell destruction and as MRI contrast agents for several years. A more recent biological application has focused...
Attempted endocytosis of nano-environment produced by colloidal lithography by human fibroblasts
Dalby, M.J., Berry, C.C., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
Control of the cells' nanoenvironment is likely to be important in the future of cell and tissue engineering. Microtopography has been shown to provide cues to cells that elicit a large range of cell...
Dalby, M.J., Riehle, M.O., Johnstone, H., Affrossman, S., Curtis, A.S.G.
Having the ability to control cell behaviour would be of great advantage in tissue engineering. One method of gaining control over cell adhesion, proliferation, guidance and differentiation is use of...
Tubes with controllable internal nanotopography
Gadegaard, N., Dalby, M.J., Riehle, M.O., Curtis, A.S.G., Affrossman, S.
No abstract available.
Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size
Dalby, M.J., Gadegaard, N., Riehle, M.O., Wilkinson, C.D.W., Curtis, A.S.G.
In order for cells to react to topography, they must be able to sense shape. When considering nano-topography, these shapes are much smaller than the cell, but still strong responses to...
Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
In designing new biomaterials, specific chemical and topographical cues will be important in guiding cell response. Filopodia are actin-driven structures produced by cells and speculated to be...
Fibroblast response to a controlled nanoenvironment produced by colloidal lithography
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
It is thought that by understanding how cells respond to topography, that better tissue engineering may be achievable. An important consideration in the cellular environment is topography. The...
Dalby, M.J., Riehle, M.O., Johnstone, H.J.H., Affrossman, S., Curtis, A.S.G.
It is becoming clear that cells do not only respond to micrometric scale topography, but may also respond to topography at the nanometric scale. Nano-fabrication methods such as electron beam...
Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography
Dalby, M.J., Giannaras, D., Riehle, M.O., Gadegaard, N., Affrossman, S., Curtis, A.S.G.
It is well known that many cell types react strongly to micro-topography. It is rapidly becoming clear than cells will also react to nano-topography. Polymer demixing is a rapid and low-cost chemical...
Dalby, M.J., Riehle, M.O., Sutherland, D.S., Agheli, H., Curtis, A.S.G.
The environment around a cell during in vitro culture is unlikely to mimic those in vivo. Preliminary experiments with nanotopography have shown that nanoscale features can strongly influence cell...
The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography
Dalby, M.J., Gadegaard, N., Wilkinson, C.D.W.
It has been known for many years that cells will react to the shape of their microenvironment. It is more recently becoming clear that cells can alter their morphology, adhesions, and cytoskeleton in...
Dalby, M.J., Hart, A., Yarwood, S.J.
Abstract: A wide variety of different cell types have been shown to respond to nanofabricated growth surfaces via the process of contact guidance, however little is known about the intracellular...
Biggs, M.J.P., Richards, R.G., McFarlane, S., Wilkinson, C.D.W., Oreffo, R.O.C., Dalby, M.J.
The surface microtexture of an orthopaedic device can regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have...
Genomic expression of mesenchymal stem cells to altered nanoscale topographies
Dalby, M.J., Andar, A., Nag, A., Affrosman, S., Tare, R., McFarlane, S., ...
The understanding of cellular response to the shape of their environment would be of benefit in the development of artificial extracellular environments for potential use in the production of...