Enzyme stability and activity at high temperatures (2008)
Daniel, R. M., Danson, M. J., Hough, D. W., Lee, C. K., Peterson, M. E., Cowan, D. A.
The effect of temperature on enzyme activity: new insights and their implications (2008)
Daniel, R. M., Danson, M. J., Eisenthal, R., Lee, C. K., Peterson, M. E.
The two established thermal properties of enzymes are their activation energy and their thermal stability. Arising from careful measurements of the thermal behaviour of enzymes, a new model, the...
The 2-oxoacid dehydrogenase multienzyme complex of Haloferax volcanii (2008)
Al-Mailem, D. M., Hough, D. W., Danson, M. J.
Those aerobic archaea whose genomes have been sequenced possess four adjacent genes that, by sequence comparisons with bacteria and eukarya, appear to encode the component enzymes of a 2-oxoacid...
The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase (2008)
Potter, J. A., Kerou, M., Lamble, H. J., Bull, S. D., Hough, D. W., Danson, M. J., ...
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and...
The dependence of enzyme activity on temperature: determination and validation of parameters (2007)
Peterson, M. E., Daniel, R. M., Danson, M. J., Eisenthal, R.
Traditionally, the dependence of enzyme activity on temperature has been described by a model consisting of two processes: the catalytic reaction defined by Delta G(cat)(double dagger), and...
Eurythermalism and the temperature dependence of enzyme activity (2007)
Lee, C. K., Daniel, R. M., Shepherd, C., Saul, D., Cary, S. C., Danson, M. J., ...
The "Equilibrium Model" has provided new tools for describing and investigating enzyme thermal adaptation. It has been shown that the effect of temperature on enzyme activity is not only governed by...
Heath, C., Posner, M. G., Aass, H. C., Upadhyay, A., Scott, D. J., Hough, D. W., ...
The aerobic archaea possess four closely spaced, adjacent genes that encode proteins showing significant sequence identities with the bacterial and eukaryal components comprising the 2-oxoacid...
Catalytic efficiency and k(cat)/K-M: a useful comparator? (2007)
Eisenthal, R., Danson, M. J., Hough, D. W.
The ratio k(cat)/K-M - often referred to as the 'specificity constant' - is a useful index for comparing the relative rates of an enzyme acting on alternative, competing substrates. However, an...
New parameters controlling the effect of temperature on enzyme activity (2007)
Daniel, R. M., Danson, M. J., Eisenthal, R., Lee, C. K., Peterson, M. E.
Arising from careful measurements of the thermal behaviour of enzymes, a new model, the Equilibrium model, has been developed to explain more fully the effects of temperature on enzymes. The model...
Milburn, C. C., Lamble, H. J., Theodossis, A., Bull, S. D., Hough, D. W., Danson, M. J., ...
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose...
The thermal behaviour of enzyme activity: implications for biotechnology (2006)
Eisenthal, R., Peterson, M. E., Daniel, R. M., Danson, M. J.
The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent...
Milburn, C. C., Lamble, H. J., Theodossis, A., Bull, S. D., Hough, D. W., Danson, M. J., ...
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose...
Lamble, H. J., Theodossis, A., Milburn, C. C., Taylor, G. L., Bull, S. D., Hough, D. W., ...
The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes...
Engineering stereocontrol into an aldolase-catalysed reaction (2005)
Lamble, H. J., Danson, M. J., Hough, D. W., Bull, S. D.
A novel thermostable aldolase has been developed for synthetic application, and substrate engineering has been used to induce stereocontrol into aldol reactions of this naturally-promiscuous enzyme.
Lamble, H. J., Theodossis, A., Milburn, C. C., Taylor, G. L., Bull, S. D., Hough, D. W., ...
The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a 'promiscuous' non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes...
Engineering stereocontrol into an aldolase-catalysed reaction (2005)
Lamble, H. J., Danson, M. J., Hough, D. W., Bull, S. D.
A novel thermostable aldolase has been developed for synthetic application, and substrate engineering has been used to induce stereocontrol into aldol reactions of this naturally-promiscuous enzyme.
A new intrinsic thermal parameter for enzymes reveals true temperature optima (2004)
Peterson, M. E., Eisenthal, R, Danson, M. J., Spence, A, Daniel, R. M.
Theodossis, A., Walden, H., Westwick, E. J., Connaris, H., Lamble, H. J., Hough, D. W., ...
The hyperthermophilic Archaea Sulfolobus solfataricus grows optimally above 80degreesC and metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway. In this pathway...
A new intrinsic thermal parameter for enzymes reveals true temperature optima (2004)
Peterson, M. E., Eisenthal, R., Danson, M. J., Spence, A., Daniel, R. M.
Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a...
Lamble, F. J., Milburn, C. C., Taylor, G. L., Hough, D. W., Danson, M. J.
An investigation has been carried out into gluconate dehydratase from the hyperthermophilic Archaeon Sulfolobus solfataricus. The enzyme has been purified from cell extracts of the organism and found...
Heath, C., Jeffries, A. C., Hough, D. W., Danson, M. J.
Those aerobic archaea whose genomes have been sequenced possess a single 4-gene operon that, by sequence comparisons with Bacteria and Eukarya, appears to encode the three component enzymes of a...
Multienzyme complexes in the Archaea: predictions from genome sequences (2004)
Danson, M. J., Morgan, D. J., Jefferies, A. C., Hough, D. W., Dyall-Smith, M. L.
Crenell, S.J., Higgins, C., Nawaz, S., Yeoh, L., Hough, D.W., ...
A bacterial thermostable citrate synthase has been analyzed to investigate the structural basis of its thermostability, and to compare such features with those previously identified in archaeal...
Wery, N., Gerike, U., Sharman, A., Chaudhuri, J. B., Hough, D. W., Danson, M. J.
Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10degreesC and continuously fed with a...
Lamble, H. J., Heyer, N. I., Bull, S. D., Hough, D. W., Danson, M. J.
The hyperthermophilic Archaeon Sulfolobus solfataricus metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway. In this pathway glucose dehydrogenase and gluconate...
Lamble, H. J., Heyer, N. I., Bull, S. D., Hough, D. W., Danson, M. J.
The hyperthermophilic Archaeon Sulfolobus solfataricus metabolizes glucose by a non-phosphorylative variant of the Entner-Doudoroff pathway. In this pathway glucose dehydrogenase and gluconate...
Karlsson, E. N., Abou-Hachem, M., Holst, O., Danson, M. J., Hough, D. W.
Two separate citrate synthases from the extremely thermophilic bacterium Rhodothermus marinus have been identified and purified. One of the enzymes is a hexameric protein and is the first...
Bell, G. S., Russell, R. J. M., Connaris, H., Hough, D. W., Danson, M. J., Taylor, G. L.
The crystal structure of citrate synthase from the thermophilic Archaeon Sulfolobus solfataricus (optimum growth temperature = 85 degreesC) has been determined, extending the number of crystal...
Cold-active citrate synthase: mutagenesis of active-site residues (2001)
Gerike, U., Danson, M. J., Hough, D. W.
A comparison of the crystal structure of the dimeric enzyme citrate synthase from the psychrophilic Arthrobacter strain DS2-3R with that of the structurally homologous enzyme from the...
The temperature optima of enzymes: a new perspective on an old phenomenon (2001)
Daniel, R. M., Danson, M. J., Eisenthal, R.
Careful analysis of the dependence of enzyme activity on assay temperature has revealed that some enzymes might have real temperature optima in which the decrease in catalytic rate at temperatures...
Genome sequence of Halobacterium species NRC-1 (2000)
Ng, W. V., Kennedy, S. P., Mahairas, G. G., Berquist, B., Pan, M., Shukla, H. D., ...
We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into...
Kurz, L. C., Drysdale, G., Riley, M., Tomar, M. A., Chen, J., Russell, R. J. M., ...
The kinetics and mechanism of the citrate synthase from a moderate thermophile, Thermoplasma acidophilum (TpCS), are compared with those of the citrate synthase from a mesophile, pig heart (PCS). All...
Jolley, K. A., Maddocks, D. G., Gyles, S. L., Mullan, Z., Tang, S. L., Dyall-Smith, M. L., ...
All Archaea catalyse the conversion of pyruvate to acetyl-CoA via a simple pyruvate oxidoreductase. This is in contrast to the Eukarya and most aerobic bacteria, which use the pyruvate dehydrogenase...
Hendry, E. J., Buchanan, C. L., Russell, R. J. M., Hough, D. W., Reeve, C. D., Danson, M. J., ...
Crystals have been grown of 2-keto-3-deoxygluconate aldolase (KDG aldolase) from the hyperthermophilic archaeon Sulfolobus solfataricus that diffract to 2.2 Angstrom resolution. The enzyme catalyses...
A microbiological survey of Montserrat Island hydrothermal biotopes (2000)
Atkinson, T., Gairns, S., Cowan, D. A., Danson, M. J., Hough, D. W., Johnson, D. B., ...
In March 1996, a survey of hydrothermal sites on the island of Montserrat was carried out. Six sites (Galway's Soufriere, Gages Upper and Lower Soufrieres, Hot Water Pond, Hot River, and Tar River...
Arnott, M. A., Michael, R. A., Thompson, C. R., Hough, D. W., Danson, M. J.
Citrate synthases from Thermoplasma acidophilum (optimal growth at 55 degreesC) and Pyrococcus furiosus (100 degreesC) are homo-dimeric enzymes that show a high degree of structural homology with...
Jolley, K A, Rapaport, E, Hough, D W, Danson, M J, Woods, W G, Dyall-Smith, M L
The gene encoding dihydrolipoamide dehydrogenase from the halophilic archaeon, Haloferax volcanii, has been subcloned and overexpressed in the parent organism by using the halophilic archaeal rRNA...
Else, A J, Danson, M J, Weitzman, P D
A simple statistical approach was used to generate predictive models of the proteolysis of multisubunit enzymes in order to correlate the loss of enzyme activity with the loss of native subunit. The...
Detection of bacterial lipoic acid. A modified gas-chromatographic-mass-spectrometric procedure.
Pratt, K J, Carles, C, Carne, T J, Danson, M J, Stevenson, K J
The detection of bacterial lipoic acid by a modified g.c.-m.s. procedure is reported. Cells were hydrolysed in HCl to release protein-bound lipoic acid, which, after extraction into benzene, was...
Smith, L D, Budgen, N, Bungard, S J, Danson, M J, Hough, D W
Glucose dehydrogenase was purified to homogeneity from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme is a tetramer of polypeptide chain Mr 38,000 +/- 3000, it is...
Dihydrolipoamide dehydrogenase from Trypanosoma brucei. Characterization and cellular location.
Danson, M J, Conroy, K, McQuattie, A, Stevenson, K J
Dihydrolipoamide dehydrogenase has been discovered in the bloodstream form of the eukaryotic African parasite, Trypanosoma brucei. The enzyme catalysed the stoichiometric oxidation of...
Else, A J, Barnes, S J, Danson, M J, Weitzman, P D
We have demonstrated that citrate synthase may be assayed by a simple, discontinuous, spectrophotometric procedure based on the measurement of oxaloacetate utilization with...
Robinson, M S, Danson, M J, Weitzman, P D
Citrate synthase was purified to homogeneity from a Gram-positive bacterium (Bacillus megaterium) for the first time. The Mr of the native enzyme was determined to be 84 000 (S.E.M. +/- 5000). Sodium...
Dihydrolipoamide dehydrogenase from halophilic archaebacteria.
Danson, M J, Eisenthal, R, Hall, S, Kessell, S R, Williams, D L
Dihydrolipoamide dehydrogenase has been discovered in the halophilic archaebacteria for the first time. The enzyme from both classical and alkaliphilic halobacteria has been investigated. (1) The...
Stanley, C J, Packman, L C, Danson, M J, Henderson, C E, Perham, R N
A simple method was developed for assessing the intramolecular coupling of active sites in the lipoate acetyltransferase (E2) component of the pyruvate dehydrogenase multienzyme complexes from...
Danson, M J, Hale, G, Perham, R N
Two lipoic acid residues on each dihydrolipoamide acetyltransferase (E2) chain of the pyruvate dehydrogenase multienzyme complex of Escherichia coli were found to undergo oxidoreduction reactions...
The reaction of two maleimides, N-ethylmaleimide and bis-(N-maleimidomethyl) ether, with the pyruvate dehydrogenase multienzyme complex of Escherichia coli in the presence of the substrate, pyruvate,...
Danson, M J, Hooper, E A, Perham, R N
The intramolecular passage of substrate between the component enzymes of the pyruvate dehydrogenase multienzyme complex of Escherichia coli was examined. A series of partly reassembled complexes,...
Buchanan, C L, Connaris, H, Danson, M J, Reeve, C D, Hough, D W
Sulfolobus solfataricus is a hyperthermophilic archaeon growing optimally at 80-85 degrees C. It metabolizes glucose via a novel non-phosphorylated Entner-Doudoroff pathway, in which the reversible...
Jolley, K A, Rapaport, E, Hough, D W, Danson, M J, Woods, W G, Dyall-Smith, M L
The gene encoding dihydrolipoamide dehydrogenase from the halophilic archaeon, Haloferax volcanii, has been subcloned and overexpressed in the parent organism by using the halophilic archaeal rRNA...
Else, A J, Danson, M J, Weitzman, P D
A simple statistical approach was used to generate predictive models of the proteolysis of multisubunit enzymes in order to correlate the loss of enzyme activity with the loss of native subunit. The...
Detection of bacterial lipoic acid. A modified gas-chromatographic-mass-spectrometric procedure.
Pratt, K J, Carles, C, Carne, T J, Danson, M J, Stevenson, K J
The detection of bacterial lipoic acid by a modified g.c.-m.s. procedure is reported. Cells were hydrolysed in HCl to release protein-bound lipoic acid, which, after extraction into benzene, was...
Smith, L D, Budgen, N, Bungard, S J, Danson, M J, Hough, D W
Glucose dehydrogenase was purified to homogeneity from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme is a tetramer of polypeptide chain Mr 38,000 +/- 3000, it is...
Dihydrolipoamide dehydrogenase from Trypanosoma brucei. Characterization and cellular location.
Danson, M J, Conroy, K, McQuattie, A, Stevenson, K J
Dihydrolipoamide dehydrogenase has been discovered in the bloodstream form of the eukaryotic African parasite, Trypanosoma brucei. The enzyme catalysed the stoichiometric oxidation of...
Else, A J, Barnes, S J, Danson, M J, Weitzman, P D
We have demonstrated that citrate synthase may be assayed by a simple, discontinuous, spectrophotometric procedure based on the measurement of oxaloacetate utilization with...
Robinson, M S, Danson, M J, Weitzman, P D
Citrate synthase was purified to homogeneity from a Gram-positive bacterium (Bacillus megaterium) for the first time. The Mr of the native enzyme was determined to be 84 000 (S.E.M. +/- 5000). Sodium...
Dihydrolipoamide dehydrogenase from halophilic archaebacteria.
Danson, M J, Eisenthal, R, Hall, S, Kessell, S R, Williams, D L
Dihydrolipoamide dehydrogenase has been discovered in the halophilic archaebacteria for the first time. The enzyme from both classical and alkaliphilic halobacteria has been investigated. (1) The...
Stanley, C J, Packman, L C, Danson, M J, Henderson, C E, Perham, R N
A simple method was developed for assessing the intramolecular coupling of active sites in the lipoate acetyltransferase (E2) component of the pyruvate dehydrogenase multienzyme complexes from...
Danson, M J, Hale, G, Perham, R N
Two lipoic acid residues on each dihydrolipoamide acetyltransferase (E2) chain of the pyruvate dehydrogenase multienzyme complex of Escherichia coli were found to undergo oxidoreduction reactions...
The reaction of two maleimides, N-ethylmaleimide and bis-(N-maleimidomethyl) ether, with the pyruvate dehydrogenase multienzyme complex of Escherichia coli in the presence of the substrate, pyruvate,...
Danson, M J, Hooper, E A, Perham, R N
The intramolecular passage of substrate between the component enzymes of the pyruvate dehydrogenase multienzyme complex of Escherichia coli was examined. A series of partly reassembled complexes,...
Buchanan, C L, Connaris, H, Danson, M J, Reeve, C D, Hough, D W
Sulfolobus solfataricus is a hyperthermophilic archaeon growing optimally at 80-85 degrees C. It metabolizes glucose via a novel non-phosphorylated Entner-Doudoroff pathway, in which the reversible...