Mycobacterium lepraemurium infection of nude athymic (nu/nu) mice.
Nude athymic (nu/nu) mice on a BALB/c background and their heterozygous euthymic litter mates (nu/+) were infected with either 10(8) or 10(6) Mycobacterium lepraemurium organisms intravenously or in...
Suppression of adoptive antituberculosis immunity by normal recipient animals.
Adoptive immunity is poorly expressed in normal syngeneic mice. This phenomenon was studied by using experimental antituberculosis immunity as a model system representing pure cell-mediated immunity....
Use of serum antibody and lysozyme levels for diagnosis of leprosy and tuberculosis.
Active tuberculosis (TB) and leprosy are difficult to diagnose early because there are few organisms to detect and the specific immune response does not distinguish between active and inactive...
Mice immunized with 10(8) live Mycobacterium lepraemurium in the footpad showed increased resistance to infection with BCG or M. tuberculosis R1Rv. This resistance could be transferred adoptively...
Induction of cell-mediated immunity to Mycobacterium leprae in mice.
The immune response of mice to armadillo-derived, irradiation-killed Mycobacterium leprae (I-ML) was investigated. Following injection of 100 microgram of I-ML into the left hind footpads of mice, a...
The immune response of random-bred mice to infection with a relatively avirulent strain of Plasmodium yoelii was measured in terms of parasitemia, splenomegaly, immediate and delayed hypersensitivity...
Development of delayed-type hypersensitivity during Mycobacterium lepraemurium infection in mice.
Various preparations of Mycobacterium lepraemurium were used to elicit delayed-type hypersensitivity in the footpad of mice infected with this organism. With a sonicated preparation of the...
Suppression of immunity to Mycobacterium lepraemurium infection.
After injection of 10(8) live Mycobacterium lepraemurium (MLM) into the left hind footpad of mice, there is development of local swelling attributable to a granuloma of the cell-mediated immunity...
Induction and expression of immunity after BCG immunization.
The induction and expression of immunity to Mycobacterium tuberculosis after BCG immunization by intravenous, subcutaneous, and pulmonary routes has been investigated in mice. The speed with which...
Induction of cell-mediated immunity to Mycobacterium lepraemurium in susceptible mice.
Lefford, M J, Patel, P J, Poulter, L W, Mackaness, G B
A mouse strain (CB6) that is highly susceptible to Mycobacterium lepraemurium was infected with 10(8) bacilli into the hind footpad. These mice developed cell-mediated immunity to M. lepraemurium, as...
The relationship between the level of delayed-type hypersensitivity (DTH) and the progression of Mycobacterium lepraemurium infection was examined after inoculation of mice with 10(8) M. lepraemurium...
Specific and nonspecific resistance in mice immunized with irradiated Myobacterium leprae.
Following subcutaneous inoculation of irradiated Mycobacterium leprae (I-ML) into the left hind footpad of mice, there was increased resistance to Listeria monocytogenes, indicative of macrophage...
Listeria pneumonitis: induction of immunity after airborne infection with Listeria monocytogenes.
Lefford, M J, Amell, L, Warner, S
After implantation of approximately 10(3) Listeria monocytogenes organisms into the lungs, mice develop an acute pneumonitis with dissemination of infection to a mediastinal lymph node (MedLN),...
Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high...
Immune Response to Mycobacterium tuberculosis in Rats
Lefford, M. J., McGregor, D. D., Mackaness, G. B.
After intravenous injection into rats, both the attenuated strain R1Rv and the virulent strain H37Rv of Mycobacterium tuberculosis grow in the liver and spleen. However, the infected rats mount a...
Macrophage activation and resistance to pulmonary tuberculosis.
Mice were vaccinated with 300 micrograms of BCG cell walls (BCG-CW) in oil-in-water emulsion intravenously or with a high or low dose of living BCG by inhalation (BCG-HD or BCG-LD, respectively). The...
Effect of Mycobacterium bovis BCG vaccination upon Mycobacterium lepraemurium infection.
Lefford, M J, Morgan, R, Logie, P S
Mice were infected with 10(8) Mycobacterium lepraemurium in the footpad (unsuppressed mice), and some of these animals were concurrently given 10(9) heat-killed M. lepraemurium intravenously...
Mycobacterium lepraemurium infection of nude athymic (nu/nu) mice.
Nude athymic (nu/nu) mice on a BALB/c background and their heterozygous euthymic litter mates (nu/+) were infected with either 10(8) or 10(6) Mycobacterium lepraemurium organisms intravenously or in...
Suppression of adoptive antituberculosis immunity by normal recipient animals.
Adoptive immunity is poorly expressed in normal syngeneic mice. This phenomenon was studied by using experimental antituberculosis immunity as a model system representing pure cell-mediated immunity....
Use of serum antibody and lysozyme levels for diagnosis of leprosy and tuberculosis.
Active tuberculosis (TB) and leprosy are difficult to diagnose early because there are few organisms to detect and the specific immune response does not distinguish between active and inactive...
Mice immunized with 10(8) live Mycobacterium lepraemurium in the footpad showed increased resistance to infection with BCG or M. tuberculosis R1Rv. This resistance could be transferred adoptively...
Induction of cell-mediated immunity to Mycobacterium leprae in mice.
The immune response of mice to armadillo-derived, irradiation-killed Mycobacterium leprae (I-ML) was investigated. Following injection of 100 microgram of I-ML into the left hind footpads of mice, a...
The immune response of random-bred mice to infection with a relatively avirulent strain of Plasmodium yoelii was measured in terms of parasitemia, splenomegaly, immediate and delayed hypersensitivity...
Development of delayed-type hypersensitivity during Mycobacterium lepraemurium infection in mice.
Various preparations of Mycobacterium lepraemurium were used to elicit delayed-type hypersensitivity in the footpad of mice infected with this organism. With a sonicated preparation of the...
Suppression of immunity to Mycobacterium lepraemurium infection.
After injection of 10(8) live Mycobacterium lepraemurium (MLM) into the left hind footpad of mice, there is development of local swelling attributable to a granuloma of the cell-mediated immunity...
Induction and expression of immunity after BCG immunization.
The induction and expression of immunity to Mycobacterium tuberculosis after BCG immunization by intravenous, subcutaneous, and pulmonary routes has been investigated in mice. The speed with which...
Induction of cell-mediated immunity to Mycobacterium lepraemurium in susceptible mice.
Lefford, M J, Patel, P J, Poulter, L W, Mackaness, G B
A mouse strain (CB6) that is highly susceptible to Mycobacterium lepraemurium was infected with 10(8) bacilli into the hind footpad. These mice developed cell-mediated immunity to M. lepraemurium, as...
The relationship between the level of delayed-type hypersensitivity (DTH) and the progression of Mycobacterium lepraemurium infection was examined after inoculation of mice with 10(8) M. lepraemurium...
Specific and nonspecific resistance in mice immunized with irradiated Myobacterium leprae.
Following subcutaneous inoculation of irradiated Mycobacterium leprae (I-ML) into the left hind footpad of mice, there was increased resistance to Listeria monocytogenes, indicative of macrophage...
Listeria pneumonitis: induction of immunity after airborne infection with Listeria monocytogenes.
Lefford, M J, Amell, L, Warner, S
After implantation of approximately 10(3) Listeria monocytogenes organisms into the lungs, mice develop an acute pneumonitis with dissemination of infection to a mediastinal lymph node (MedLN),...
Random-bred mice were immunized with a nonliving antigen prepared from mixed-blood forms of Plasmodium berghei, strain NYU-2, in combination with Corynebacterium parvum and/or living BCG. A high...
Immune Response to Mycobacterium tuberculosis in Rats
Lefford, M. J., McGregor, D. D., Mackaness, G. B.
After intravenous injection into rats, both the attenuated strain R1Rv and the virulent strain H37Rv of Mycobacterium tuberculosis grow in the liver and spleen. However, the infected rats mount a...
Macrophage activation and resistance to pulmonary tuberculosis.
Mice were vaccinated with 300 micrograms of BCG cell walls (BCG-CW) in oil-in-water emulsion intravenously or with a high or low dose of living BCG by inhalation (BCG-HD or BCG-LD, respectively). The...
Effect of Mycobacterium bovis BCG vaccination upon Mycobacterium lepraemurium infection.
Lefford, M J, Morgan, R, Logie, P S
Mice were infected with 10(8) Mycobacterium lepraemurium in the footpad (unsuppressed mice), and some of these animals were concurrently given 10(9) heat-killed M. lepraemurium intravenously...
The effect of inoculum size on the immune response to BCG infection in mice
In mice infected with BCG the rate of development of resistance to L. monocytogenes was related, and the growth of BCG in the liver and spleen was inversely related, to the size of the infecting...
Properties of lymphocytes which confer adoptive immunity to tuberculosis in rats
Lefford, M. J., McGregor, D. D., Mackaness, G. B.
Following immunization of rats with BCG there was added to thoracic duct lymph a population of sensitized lymphocytes which conferred adoptive immunity to tuberculosis upon syngeneic recipients. At...
The lymphocyte mediators of delayed hypersensitivity: the early phase cells.
Inbred rats were immunized with living BCG and lymphocytes which mediate tuberculin DTH and anti-tuberculosis immunity were found 10 days later in the draining lymph nodes, thoracic duct, blood,...
Acute peritoneal exudates were raised in rats that had been immunized by BCG, in order to draw into the peritoneal cavity sensitized immunoblasts. The peritoneal exudate cells (PEC) were harvested 24...
Rats were immunized with living BCG and acute peritoneal exudates were induced on the ninth day of infection. The peritoneal exudate cells (PEC), that confer adoptive anti-tuberculosis immunity and...
THE HOST RESPONSE TO CALMETTE-GUÉRIN BACILLUS INFECTION IN MICE
Blanden, R. V., Lefford, M. J., Mackaness, G. B.
Heterologous organisms (L. monocytogenes and S. typhimurium) were used to study the rate of development, magnitude, and persistence of the antimicrobial resistance engendered in mice by vaccination...