Peredo, C, O'Reilly, L, Gray, K, Roth, M J
A series of 22 chimeric envelope (env) genes were generated between the ecotropic Moloney murine leukemia virus and the amphotropic 4070A isolate. The chimeric envelopes were expressed within the...
Coordinated disintegration reactions mediated by Moloney murine leukemia virus integrase.
Donzella, G A, Jonsson, C B, Roth, M J
The protein-DNA and protein-protein interactions important for function of the integrase (IN) protein of Moloney murine leukemia virus (M-MuLV) were investigated by using a coordinated-disintegration...
Jonsson, C B, Donzella, G A, Gaucan, E, Smith, C M, Roth, M J
Retroviral integrases perform two catalytic steps, 3' processing and strand transfer, that result in the stable insertion of the retroviral DNA into the host genome. Mutant M-MuLV integrases were...
Smith, J S, Gritsman, K, Roth, M J
Previous studies showed that an isolated human immunodeficiency virus type 1 (HIV-1) RNase H domain expressed as a fusion protein is highly active in Mn2+, but activity was dependent on a...
Mutational analysis of the envelope gene of Moloney murine leukemia virus.
The env gene products of Moloney murine leukemia virus are required for binding and entry of the virus into the target cell. Thirty-three linker insertion mutations were constructed throughout the...
Purification and characterization of an active human immunodeficiency virus type 1 RNase H domain.
We have expressed and purified from Escherichia coli a human immunodeficiency virus type 1 (HIV-1) RNase H domain consisting of amino acids 400 to 560 of reverse transcriptase with either an N- or...
Retroviral integrases mediate site-specific endonuclease and transesterification reactions in the absence of exogenous energy. The basis for the sequence specificity in these integrase-viral DNA...
Donzella, G A, Jonsson, C B, Roth, M J
The disintegration activity of Moloney murine leukemia virus (M-MuLV) integrase (IN) was investigated through structural and sequence modifications of a Y substrate that resembles an integration...
The integration protein (IN) is required for retrovirus DNA integration into the host DNA. The function of the C terminus of the Moloney murine leukemia virus IN protein was examined. The terminal 28...
The role of the N-linked glycosylation sites in the major envelope glycoprotein, SU (gp70), of Moloney murine leukemia virus has been examined. By using site-specific oligonucleotide-directed...
Roth, M J, Schwartzberg, P, Tanese, N, Goff, S P
The 3' terminus of the pol gene of Moloney murine leukemia virus encodes the integration (IN) protein, required for the establishment of the integrated provirus. A series of six linker insertion...
Analysis of long terminal repeat circle junctions of human immunodeficiency virus type 1.
Smith, J S, Kim, S Y, Roth, M J
Circle junctions of unintegrated human immunodeficiency virus type 1 strain IIIB were analyzed after polymerase chain reaction amplification. Among the 28 colonies sequenced, eight unique circle...
Tanese, N, Roth, M J, Goff, S P
Portions of the pol gene of Moloney murine leukemia virus (MuLV) were expressed as fusion proteins in Escherichia coli, and the purified proteins were used to elicit antibodies in Escherichia coli,...
Peredo, C, O'Reilly, L, Gray, K, Roth, M J
A series of 22 chimeric envelope (env) genes were generated between the ecotropic Moloney murine leukemia virus and the amphotropic 4070A isolate. The chimeric envelopes were expressed within the...
Coordinated disintegration reactions mediated by Moloney murine leukemia virus integrase.
Donzella, G A, Jonsson, C B, Roth, M J
The protein-DNA and protein-protein interactions important for function of the integrase (IN) protein of Moloney murine leukemia virus (M-MuLV) were investigated by using a coordinated-disintegration...
Jonsson, C B, Donzella, G A, Gaucan, E, Smith, C M, Roth, M J
Retroviral integrases perform two catalytic steps, 3' processing and strand transfer, that result in the stable insertion of the retroviral DNA into the host genome. Mutant M-MuLV integrases were...
Smith, J S, Gritsman, K, Roth, M J
Previous studies showed that an isolated human immunodeficiency virus type 1 (HIV-1) RNase H domain expressed as a fusion protein is highly active in Mn2+, but activity was dependent on a...
Mutational analysis of the envelope gene of Moloney murine leukemia virus.
The env gene products of Moloney murine leukemia virus are required for binding and entry of the virus into the target cell. Thirty-three linker insertion mutations were constructed throughout the...
Purification and characterization of an active human immunodeficiency virus type 1 RNase H domain.
We have expressed and purified from Escherichia coli a human immunodeficiency virus type 1 (HIV-1) RNase H domain consisting of amino acids 400 to 560 of reverse transcriptase with either an N- or...
Retroviral integrases mediate site-specific endonuclease and transesterification reactions in the absence of exogenous energy. The basis for the sequence specificity in these integrase-viral DNA...
Donzella, G A, Jonsson, C B, Roth, M J
The disintegration activity of Moloney murine leukemia virus (M-MuLV) integrase (IN) was investigated through structural and sequence modifications of a Y substrate that resembles an integration...
The integration protein (IN) is required for retrovirus DNA integration into the host DNA. The function of the C terminus of the Moloney murine leukemia virus IN protein was examined. The terminal 28...
The role of the N-linked glycosylation sites in the major envelope glycoprotein, SU (gp70), of Moloney murine leukemia virus has been examined. By using site-specific oligonucleotide-directed...
Roth, M J, Schwartzberg, P, Tanese, N, Goff, S P
The 3' terminus of the pol gene of Moloney murine leukemia virus encodes the integration (IN) protein, required for the establishment of the integrated provirus. A series of six linker insertion...
Analysis of long terminal repeat circle junctions of human immunodeficiency virus type 1.
Smith, J S, Kim, S Y, Roth, M J
Circle junctions of unintegrated human immunodeficiency virus type 1 strain IIIB were analyzed after polymerase chain reaction amplification. Among the 28 colonies sequenced, eight unique circle...
Tanese, N, Roth, M J, Goff, S P
Portions of the pol gene of Moloney murine leukemia virus (MuLV) were expressed as fusion proteins in Escherichia coli, and the purified proteins were used to elicit antibodies in Escherichia coli,...
Emmert-Buck, M. R., Roth, M. J., Zhuang, Z., Campo, E., Rozhin, J., Sloane, B. F., ...
Gelatinase A (MMP-2) and cathepsin B are proteinases which have been proposed to participate in human tumor invasion and metastasis. Precise quantitation of the activity of these enzymes in invading...
Wang, G-Q, Abnet, C C, Shen, Q, Lewin, K J, Sun, X-D, Roth, M J, ...
Background: Oesophageal squamous cell carcinoma (OSCC) has a very poor prognosis, which is largely due to late diagnosis. Successful early detection strategies will require identification of...
Risk factors for oesophageal squamous dysplasia in adult inhabitants of a high risk region of China
Wei, W-Q, Abnet, C C, Lu, N, Roth, M J, Wang, G-Q, Dye, B A, ...
Background: Oesophageal squamous cell carcinoma (OSCC) is a common cancer worldwide and has a very high mortality rate. Squamous dysplasia is the precursor lesion for OSCC and it can be seen during...