Scientific Commons: M. K. Gorny

Production of site-selected neutralizing human monoclonal antibodies against the third variable domain of the human immunodeficiency virus type 1 envelope glycoprotein.

Gorny, M K, Xu, J Y, Gianakakos, V, Karwowska, S, Williams, C, Sheppard, H W, ...

Cell lines secreting IgG1 human monoclonal antibodies (mAb) to the envelope glycoprotein, gp120, of human immunodeficiency virus (HIV) have been produced by transformation of peripheral blood cells...

Human monoclonal antibodies to the human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 enhance HIV-1 infection in vitro.

Robinson, W E, Kawamura, T, Gorny, M K, Lake, D, Xu, J Y, Matsumoto, Y, ...

Three of 16 human monoclonal antibodies (hu-mAbs) enhanced human immunodeficiency virus type 1 (HIV-1) infection of MT-2 target cells by means of a mechanism that is dependent on complement. Enhanced...

Serotyping of primary human immunodeficiency virus type 1 isolates from diverse geographic locations by flow cytometry.

Zolla-Pazner, S, O'Leary, J, Burda, S, Gorny, M K, Kim, M, Mascola, J, ...

The immunologic relatedness of the various human immunodeficiency virus type 1 (HIV-1) clades was determined with 13 human anti-HIV-1 monoclonal antibodies (MAbs) to six immunogenic regions of the...

Envelope glycoproteins from human immunodeficiency virus types 1 and 2 and simian immunodeficiency virus can use human CCR5 as a coreceptor for viral entry and make direct CD4-dependent interactions with this chemokine receptor.

Hill, C M, Deng, H, Unutmaz, D, Kewalramani, V N, Bastiani, L, Gorny, M K, ...

Several members of the chemokine receptor family have recently been identified as coreceptors, with CD4, for entry of human immunodeficiency virus type 1 (HIV-1) into target cells. In this report, we...

Synergistic neutralization of human immunodeficiency virus type 1 by combinations of human monoclonal antibodies.

Laal, S, Burda, S, Gorny, M K, Karwowska, S, Buchbinder, A, Zolla-Pazner, S

The ability of antibodies to the V3 region and the CD4-binding domain (CD4bd) of human immunodeficiency virus type 1 (HIV-1) to act in synergy to neutralize HIV has been demonstrated previously....

Neutralization of primary human immunodeficiency virus type 1 isolates by the broadly reactive anti-V3 monoclonal antibody, 447-52D.

Conley, A J, Gorny, M K, Kessler, J A, Boots, L J, Ossorio-Castro, M, Koenig, S, ...

Human monoclonal antibody 447-52D binds to the V3 determinant of the human immunodeficiency virus type 1 (HIV-1) gp120 external glycoprotein. Its binding requires the expression of the GPxR sequence...

Human anti-V2 monoclonal antibody that neutralizes primary but not laboratory isolates of human immunodeficiency virus type 1.

Gorny, M K, Moore, J P, Conley, A J, Karwowska, S, Sodroski, J, Williams, C, ...

A human immunoglobulin G1 lambda monoclonal antibody (MAb), 697-D, was developed that recognizes the V2 region of human immunodeficiency virus type 1 (HIV-1) gp120. Substitutions at amino acid...

Neutralization of diverse human immunodeficiency virus type 1 variants by an anti-V3 human monoclonal antibody.

Gorny, M K, Conley, A J, Karwowska, S, Buchbinder, A, Xu, J Y, Emini, E A, ...

The third variable region (V3) of the HIV-1 gp120 envelope glycoprotein is thought to induce potent neutralizing antibodies which are generally defined as type specific and reactive with individual...

Two immunodominant domains of gp41 bind antibodies which enhance human immunodeficiency virus type 1 infection in vitro.

Robinson, W E, Gorny, M K, Xu, J Y, Mitchell, W M, Zolla-Pazner, S

Four of eight human monoclonal antibodies (huMAbs) to gp41 were identified which could enhance human immunodeficiency virus type 1 (HIV-1) infection in vitro by complement-mediated antibody-dependent...

Epitope mapping of two immunodominant domains of gp41, the transmembrane protein of human immunodeficiency virus type 1, using ten human monoclonal antibodies.

Xu, J Y, Gorny, M K, Palker, T, Karwowska, S, Zolla-Pazner, S

Immunogenic regions of the gp41 transmembrane protein of human immunodeficiency virus type 1 (HIV-1) were previously mapped by examining polyclonal sera from HIV-infected patients and rodent...

Oligomeric structure of gp41, the transmembrane protein of human immunodeficiency virus type 1.

Pinter, A, Honnen, W J, Tilley, S A, Bona, C, Zaghouani, H, Gorny, M K, ...

We characterized the structural forms of the human immunodeficiency virus env-encoded proteins with a panel of monoclonal and polyclonal antibodies. Western blot (immunoblot) assays with antibodies...

Generation of human monoclonal antibodies to human immunodeficiency virus.

Gorny, M K, Gianakakos, V, Sharpe, S, Zolla-Pazner, S

Based on the finding that cells producing antibodies to human immunodeficiency virus (HIV) circulate in the peripheral blood of HIV-infected individuals, attempts were made to immortalize such B...

Human immunodeficiency virus-infected T cells and monocytes are killed by monoclonal human anti-gp41 antibodies coupled to ricin A chain.

Till, M A, Zolla-Pazner, S, Gorny, M K, Patton, J S, Uhr, J W, Vitetta, E S

Two human monoclonal antibodies specific for the envelope glycoprotein (gp), gp41, of the human immunodeficiency virus were conjugated to deglycosylated ricin A chain. These immunotoxins killed human...

Factors Determining the Breadth and Potency of Neutralization by V3-Specific Human Monoclonal Antibodies Derived from Subjects Infected with Clade A or Clade B Strains of Human Immunodeficiency Virus Type 1

Krachmarov, C. P., Honnen, W. J., Kayman, S. C., Gorny, M. K., Zolla-Pazner, S., Pinter, Abraham

The neutralizing activities of anti-V3 antibodies for HIV-1 isolates is affected both by sequence variation within V3 and by epitope masking by the V1/V2 domain. To analyze the relative contribution...