M. L. Hammarskjöld

A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.

Bray, M, Prasad, S, Dubay, J W, Hunter, E, Jeang, K T, Rekosh, D, ...

Replication of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev protein. This protein acts in concert with the cis-acting rev-responsive element present in intron-containing...

U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced.

Lu, X B, Heimer, J, Rekosh, D, Hammarskjöld, M L

rev-regulated expression of HIV-1 envelope proteins from a simian virus 40 late replacement vector was found to be dependent on the presence of a 5' splice site in the env mRNA in spite of the fact...

Characterization of deletion mutations in the capsid region of human immunodeficiency virus type 1 that affect particle formation and Gag-Pol precursor incorporation.

Srinivasakumar, N, Hammarskjöld, M L, Rekosh, D

The core of human immunodeficiency virus type 1 is derived from two precursor polyproteins, Pr55gag and Pr160gag-pol. The Gag precursor can assemble into immature virus-like particles when expressed...

The effect of viral regulatory protein expression on gene delivery by human immunodeficiency virus type 1 vectors produced in stable packaging cell lines.

Srinivasakumar, N, Chazal, N, Helga-Maria, C, Prasad, S, Hammarskjöld, M L, Rekosh, D

We describe the generation of stable human immunodeficiency virus type 1 (HIV-1)-packaging lines that constitutively express high levels of HIV-1 structural proteins in either a Rev-dependent or a...

A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA.

Ernst, R K, Bray, M, Rekosh, D, Hammarskjöld, M L

A common feature of gene expression in all retroviruses is that unspliced, intron-containing RNA is exported to the cytoplasm despite the fact that cellular RNAs which contain introns are usually...

Human immunodeficiency virus env expression becomes Rev-independent if the env region is not defined as an intron.

Hammarskjöld, M L, Li, H, Rekosh, D, Prasad, S

The human immunodeficiency virus (HIV) Rev protein functions to facilitate export of intron-containing HIV mRNA from the nucleus to the cytoplasm. We have previously shown that splice site...

Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles.

Mak, J, Jiang, M, Wainberg, M A, Hammarskjöld, M L, Rekosh, D, Kleiman, L

COS-7 cells transfected with human immunodeficiency virus type 1 (HIV-1) proviral DNA produce virus in which three tRNA species are most abundant in the viral tRNA population. These tRNAs have been...

Epstein-Barr virus latent membrane protein transactivates the human immunodeficiency virus type 1 long terminal repeat through induction of NF-kappa B activity.

Hammarskjöld, M L, Simurda, M C

The Epstein-Barr virus latent membrane protein (LMP) is an integral membrane protein that is expressed in cells latently infected with the virus. LMP is believed to play an important role in...

Requirements for incorporation of Pr160gag-pol from human immunodeficiency virus type 1 into virus-like particles.

Smith, A J, Srinivasakumar, N, Hammarskjöld, M L, Rekosh, D

The roles of the human immunodeficiency virus precursor polyproteins Pr55gag and Pr160gag-pol in viral core assembly were studied in CMT3-COS cells. To do this, the precursors were expressed...

Identification of a cis-acting element in human immunodeficiency virus type 2 (HIV-2) that is responsive to the HIV-1 rev and human T-cell leukemia virus types I and II rex proteins.

Lewis, N, Williams, J, Rekosh, D, Hammarskjöld, M L

A simian virus 40 late replacement vector encoding human immunodeficiency virus type 1 (HIV-1) gp120 (pGP120) was used to define a region within the HIV-2 genome that could work as a rev-responsive...

Human immunodeficiency virus type 1 Pr55gag and Pr160gag-pol expressed from a simian virus 40 late replacement vector are efficiently processed and assembled into viruslike particles.

Smith, A J, Cho, M I, Hammarskjöld, M L, Rekosh, D

Human immunodeficiency virus type 1 (HIV-1) gag and pol genes were expressed by using fragments of the BH10 clone of HIV inserted into a simian virus 40 late replacement vector. An initial construct...

Regulation of human immunodeficiency virus env expression by the rev gene product.

Hammarskjöld, M L, Heimer, J, Hammarskjöld, B, Sangwan, I, Albert, L, Rekosh, D

A single simian virus 40 late replacement vector which expresses both the rev and envelope (env) genes of human immunodeficiency virus was used to examine the mechanism underlying the dependence of...

Coexpression of human immunodeficiency virus envelope proteins and tat from a single simian virus 40 late replacement vector.

Rekosh, D, Nygren, A, Flodby, P, Hammarskjöld, M L, Wigzell, H

A Sal I-Xho I fragment containing the genes encoding tat, art, and the envelope proteins from the BH10 clone of human immunodeficiency virus (HIV) was inserted into a simian virus 40 (SV40)-based...

Mutations in the principal neutralization determinant of human immunodeficiency virus type 1 affect syncytium formation, virus infectivity, growth kinetics, and neutralization.

Grimaila, R J, Fuller, B A, Rennert, P D, Nelson, M B, Hammarskjöld, M L, Potts, B, ...

The principal neutralization determinant (PND) of human immunodeficiency virus type 1 envelope glycoprotein gp120 contains a conserved GPG sequence. The effects of a 29-amino-acid deletion of most of...

Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.

Winberg, G, Hammarskjöld, M L

A new method for isolating DNA from agarose gels is described. The method involves the simultaneous transfer of all DNA-fragments from an agarose slab gel onto DEAE-cellulose paper and the elution of...

The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.

Pasquinelli, A E, Ernst, R K, Lund, E, Grimm, C, Zapp, M L, Rekosh, D, ...

The constitutive transport elements (CTEs) of type D retroviruses are cis-acting elements that promote nuclear export of incompletely spliced mRNAs. Unlike the Rev response element (RRE) of human...

A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.

Bray, M, Prasad, S, Dubay, J W, Hunter, E, Jeang, K T, Rekosh, D, ...

Replication of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev protein. This protein acts in concert with the cis-acting rev-responsive element present in intron-containing...

U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced.

Lu, X B, Heimer, J, Rekosh, D, Hammarskjöld, M L

rev-regulated expression of HIV-1 envelope proteins from a simian virus 40 late replacement vector was found to be dependent on the presence of a 5' splice site in the env mRNA in spite of the fact...

Characterization of deletion mutations in the capsid region of human immunodeficiency virus type 1 that affect particle formation and Gag-Pol precursor incorporation.

Srinivasakumar, N, Hammarskjöld, M L, Rekosh, D

The core of human immunodeficiency virus type 1 is derived from two precursor polyproteins, Pr55gag and Pr160gag-pol. The Gag precursor can assemble into immature virus-like particles when expressed...

The effect of viral regulatory protein expression on gene delivery by human immunodeficiency virus type 1 vectors produced in stable packaging cell lines.

Srinivasakumar, N, Chazal, N, Helga-Maria, C, Prasad, S, Hammarskjöld, M L, Rekosh, D

We describe the generation of stable human immunodeficiency virus type 1 (HIV-1)-packaging lines that constitutively express high levels of HIV-1 structural proteins in either a Rev-dependent or a...

A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA.

Ernst, R K, Bray, M, Rekosh, D, Hammarskjöld, M L

A common feature of gene expression in all retroviruses is that unspliced, intron-containing RNA is exported to the cytoplasm despite the fact that cellular RNAs which contain introns are usually...

Human immunodeficiency virus env expression becomes Rev-independent if the env region is not defined as an intron.

Hammarskjöld, M L, Li, H, Rekosh, D, Prasad, S

The human immunodeficiency virus (HIV) Rev protein functions to facilitate export of intron-containing HIV mRNA from the nucleus to the cytoplasm. We have previously shown that splice site...

Role of Pr160gag-pol in mediating the selective incorporation of tRNA(Lys) into human immunodeficiency virus type 1 particles.

Mak, J, Jiang, M, Wainberg, M A, Hammarskjöld, M L, Rekosh, D, Kleiman, L

COS-7 cells transfected with human immunodeficiency virus type 1 (HIV-1) proviral DNA produce virus in which three tRNA species are most abundant in the viral tRNA population. These tRNAs have been...

Epstein-Barr virus latent membrane protein transactivates the human immunodeficiency virus type 1 long terminal repeat through induction of NF-kappa B activity.

Hammarskjöld, M L, Simurda, M C

The Epstein-Barr virus latent membrane protein (LMP) is an integral membrane protein that is expressed in cells latently infected with the virus. LMP is believed to play an important role in...

Requirements for incorporation of Pr160gag-pol from human immunodeficiency virus type 1 into virus-like particles.

Smith, A J, Srinivasakumar, N, Hammarskjöld, M L, Rekosh, D

The roles of the human immunodeficiency virus precursor polyproteins Pr55gag and Pr160gag-pol in viral core assembly were studied in CMT3-COS cells. To do this, the precursors were expressed...

Identification of a cis-acting element in human immunodeficiency virus type 2 (HIV-2) that is responsive to the HIV-1 rev and human T-cell leukemia virus types I and II rex proteins.

Lewis, N, Williams, J, Rekosh, D, Hammarskjöld, M L

A simian virus 40 late replacement vector encoding human immunodeficiency virus type 1 (HIV-1) gp120 (pGP120) was used to define a region within the HIV-2 genome that could work as a rev-responsive...

Human immunodeficiency virus type 1 Pr55gag and Pr160gag-pol expressed from a simian virus 40 late replacement vector are efficiently processed and assembled into viruslike particles.

Smith, A J, Cho, M I, Hammarskjöld, M L, Rekosh, D

Human immunodeficiency virus type 1 (HIV-1) gag and pol genes were expressed by using fragments of the BH10 clone of HIV inserted into a simian virus 40 late replacement vector. An initial construct...

Regulation of human immunodeficiency virus env expression by the rev gene product.

Hammarskjöld, M L, Heimer, J, Hammarskjöld, B, Sangwan, I, Albert, L, Rekosh, D

A single simian virus 40 late replacement vector which expresses both the rev and envelope (env) genes of human immunodeficiency virus was used to examine the mechanism underlying the dependence of...

Coexpression of human immunodeficiency virus envelope proteins and tat from a single simian virus 40 late replacement vector.

Rekosh, D, Nygren, A, Flodby, P, Hammarskjöld, M L, Wigzell, H

A Sal I-Xho I fragment containing the genes encoding tat, art, and the envelope proteins from the BH10 clone of human immunodeficiency virus (HIV) was inserted into a simian virus 40 (SV40)-based...

Mutations in the principal neutralization determinant of human immunodeficiency virus type 1 affect syncytium formation, virus infectivity, growth kinetics, and neutralization.

Grimaila, R J, Fuller, B A, Rennert, P D, Nelson, M B, Hammarskjöld, M L, Potts, B, ...

The principal neutralization determinant (PND) of human immunodeficiency virus type 1 envelope glycoprotein gp120 contains a conserved GPG sequence. The effects of a 29-amino-acid deletion of most of...

Isolation of DNA from agarose gels using DEAE-paper. Application to restriction site mapping of adenovirus type 16 DNA.

Winberg, G, Hammarskjöld, M L

A new method for isolating DNA from agarose gels is described. The method involves the simultaneous transfer of all DNA-fragments from an agarose slab gel onto DEAE-cellulose paper and the elution of...

The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.

Pasquinelli, A E, Ernst, R K, Lund, E, Grimm, C, Zapp, M L, Rekosh, D, ...

The constitutive transport elements (CTEs) of type D retroviruses are cis-acting elements that promote nuclear export of incompletely spliced mRNAs. Unlike the Rev response element (RRE) of human...