M. Leutenegger

Publication List Details

Period

2006 - 2008

Number

33

Co-Authors

Dual-color Total Internal Reflection Fluorescence Fluctuation Spectroscopy (2008)

Leutenegger, M., Blom, H., Widengren, J., Eggeling, C., Gösch, M., Lasser, T.

We developed a dual-color total internal reflection fluorescence (TIRF) system for single molecule imaging and fluorescence fluctuation spectroscopy (FFS). For a performance analysis, we measured a...

Dual-color Total Internal Reflection Fluorescence Fluctuation Spectroscopy (2008)

Leutenegger, M., Blom, H., Widengren, J., Eggeling, C., Gösch, M., Lasser, T.

We developed a dual-color total internal reflection fluorescence (TIRF) system for single molecule imaging and fluorescence fluctuation spectroscopy (FFS). For a performance analysis, we measured a...

Triplet imaging contrast by modulated excitation (2008)

Geissbühler, M., Spielmann, T., Vulliamy, R., Leutenegger, M., Märki, I., Sandén, T., ...

A novel imaging method employing the different kinetics of the energetic states of fluorescent markers is presented, where the contrast depends on the triplet kinetics. An attractive feature of...

Fast focus field calculations (2008)

Leutenegger, M., Geissbühler, M., Märki, I., Leitgeb, R. A., Lasser, T.

We present a method for fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye...

Imaging of G protein-coupled receptors in solid-supported planar membranes at the single molecule level (2008)

Märki, I., Leutenegger, M., Geissbühler, M., Robelek, R., Sinner, E.K., Lasser, T.

Odorant receptors are an excellent example of natural superiority in specifically binding specific, small and hydrophobic molecules. They are of particular interest in the development of a sensor...

Fast focus field calculations (2008)

Leutenegger, M., Geissbühler, M., Märki, I., Leitgeb, R. A., Lasser, T.

We present a method for fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye...

Imaging of G protein-coupled receptors in solid-supported planar membranes at the single molecule level (2008)

Märki, I., Leutenegger, M., Geissbühler, M., Robelek, R., Sinner, E.K., Lasser, T.

Odorant receptors are an excellent example of natural superiority in binding specific, small and hydrophobic molecules, and therefore are of particular interest in the development of a sensor...

In vitro Synthesis of GPCRs in a Solid Supported Planar Lipid Membrane System (2008)

Robelek, R., Leutenegger, M., Wiltschi, B., Lemker, E. S., Oesterhelt, D., Lasser, T., ...

Membrane protein research depends largely on the availability of functional protein in an instrumental set- up for application of biophysical investigations. We used an in vitro synthesis strategy in...

Fast focus field calculations (2008)

Leutenegger, M., Rao, R., Leitgeb, R. A., Lasser, T.

We present a fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye diffraction integral...

Single molecule detection at surfaces (2008)

Leutenegger, M., Hassler, K., Rigler, P., Bilenca, A., Lasser, T.

Fluorescence fluctuation spectroscopy based on an evanescent field excitation scheme leads to an alternative concept for single molecule detection with interesting experimental features. This concept...

Focused electron beam induced deposition of a periodic transparent nano-optic pattern (2006)

Perentes, A., Bachmann, A., Leutenegger, M., Utke, I., Sandu, C., Hoffmann, P.

In this paper we describe the fabrication of a two-dimensional photonic band gap structure by focused electron beam induced deposition. Structure geometry is computed in order to create a...

Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule (2006)

Hassler, K., Leutenegger, M., Rigler, P., Rao, R., Rigler, R., Gosch, M., ...

We designed a fluorescence correlation spectroscopy (FCS) system for measurements on surfaces. The system consists of an objective-type total internal reflection fluorescence (TIRF) microscopy setup,...

Multiple scattering in optical coherence tomography. I. Investigation and modeling (2006)

Karamata, B., Laubscher, M., Leutenegger, M., Bourquin, S., Lasser, T., Lambelet, P.

We present a new model of optical coherence tomography (OCT) taking into account multiple scattering. A theoretical analysis and experimental investigation reveals that in OCT, despite multiple...

Multiple scattering in optical coherence tomography. II. Experimental and theoretical investigation of cross talk in wide-field optical coherence tomography (2006)

Karamata, B., Leutenegger, M., Laubscher, M., Bourquin, S., Lasser, T., Lambelet, P.

We present a comprehensive study of multiple-scattering effects in wide-field optical coherence tomography (OCT) realized with spatially coherent illumination. Imaging a sample made of a cleaved...

Fabrication of arrays of sub-wavelength nano-apertures in an optically thick gold layer on glass slides for optical studies (2006)

Perentes, A., Utke, I., Dwir, B., Leutenegger, M., Lasser, T., Hoffmann, P., ...

We report on the fabrication of two-dimensional arrays of nano-optical apertures in gold layers by electron beam lithography (EBL) on a transparent glass substrate. 30 x 30 μ m(2) large arrays of...

Prism-based multicolor fluorescence correlation spectrometer (2006)

Hwang, L. C., Leutenegger, M., Gösch, M., Lasser, T., Rigler, P., Meier, W., ...

We report the design and application of a prism-based detection system for fluorescence (cross) correlation spectroscopy. The system utilizes a single laser wavelength for the simultaneous excitation...

Dual-color total internal reflection fluorescence cross- correlation spectroscopy (2006)

Leutenegger, M., Blom, H., Widengren, J., Eggeling, C., Gösch, M., Leitgeb, R.A., ...

We present the development and first application of a novel dual-color total internal reflection (TIR) fluorescence system for single-molecule coincidence analysis and fluorescence cross-correlation...

Confining the sampling volume for Fluorescence Correlation Spectroscopy using a sub-wavelength sized aperture (2006)

Leutenegger, M., Gösch, M., Perentes, A., Hoffmann, P., Martin, O. J. F., Lasser, T.

For the observation of single molecule dynamics with fluorescence fluctuation spectroscopy (FFS) very low fluorophore concentrations are necessary. For in vitro measurements, this requirement is easy...