Chronic cobalamin inactivation impairs folate polyglutamate synthesis in the rat.
Perry, J, Chanarin, I, Deacon, R, Lumb, M
Nitrous oxide, by inactivating cobalamin in vivo, produces a suitable animal model for cobalamin 'deficiency.' The synthesis of folate polyglutamate with tetrahydrofolate as substrate is severely...
Deacon, R, Chanarin, I, Lumb, M, Perry, J
The activity of the two folate dependent enzymes supplying carbon 2 and carbon 8 of the purine nucleus was assayed in the bone marrow of rats and man, as well as in rat livers. The activity of both...
Methylation of DNA in megaloblastic anaemia.
Perry, J, Chanarin, I, Deacon, R, Lumb, M
Methylation of cytosine residues in DNA samples, collected before and serially after cobalamin treatment from patients with cobalamin deficiency, was studied using restriction endonucleases Hpa II...
Regulation of 5-methyltetrahydrofolate synthesis.
Lumb, M, Bottiglieri, T, Deacon, R, Perry, J, Chanarin, I
After an intraperitoneal injection of 100 mumol of methionine to rats, there is rapid oxidation of the methyl group of hepatic 5-methyltetrahydrofolate to formate and CO2. Recovery of the...
Oxidation of 5-methyltetrahydrofolate in cobalamin-inactivated rats.
Lumb, M, Deacon, R, Perry, J, Chanarin, I
Rats were exposed to nitrous oxide, which inactivates cob(I)alamin (Cbl). As in air-breathing rats methionine administration led to the conversion of hepatic 5-methyltetrahydrofolate (MeH4 folate)...
In vivo oxidation of the methyl group of hepatic 5-methyltetrahydrofolate.
Lumb, M, Chanarin, I, Deacon, R, Perry, J
Methionine given parenterally to rats caused rapid disappearance of methyltetrahydrofolate from the liver and a corresponding rise in tetrahydrofolate and formyl-tetrahydrofolate concentrations. When...
Deacon, R, Perry, J, Lumb, M, Chanarin, I
N2O oxidizes and inactivates cob[I]alamin, and animals exposed in this way serve as models for cobalamin 'deficiency'. Such animals show a fall in activity of glycinamide ribotide transformylase and...
Folate polyglutamate synthetase activity in the cobalamin-inactivated rat.
Perry, J, Chanarin, I, Deacon, R, Lumb, M
Exposure to N2O inactivates cob[I]alamin and interferes with the activity of methionine synthetase, of which cob[I]alamin is a coenzyme. Less directly, it stops the formation of folate polyglutamate...
Lumb, M, Deacon, R, Perry, J, Chanarin, I, Minty, B, Halsey, M J, ...
Rats exposed to N20 show a decrease in liver folate to about 25% of the initial value after 10 days. There is a transient increase in the amount of 5-methylterrahydropteroylpolyglutamate in the first...
Chronic cobalamin inactivation impairs folate polyglutamate synthesis in the rat.
Perry, J, Chanarin, I, Deacon, R, Lumb, M
Nitrous oxide, by inactivating cobalamin in vivo, produces a suitable animal model for cobalamin 'deficiency.' The synthesis of folate polyglutamate with tetrahydrofolate as substrate is severely...
Deacon, R, Chanarin, I, Lumb, M, Perry, J
The activity of the two folate dependent enzymes supplying carbon 2 and carbon 8 of the purine nucleus was assayed in the bone marrow of rats and man, as well as in rat livers. The activity of both...
Methylation of DNA in megaloblastic anaemia.
Perry, J, Chanarin, I, Deacon, R, Lumb, M
Methylation of cytosine residues in DNA samples, collected before and serially after cobalamin treatment from patients with cobalamin deficiency, was studied using restriction endonucleases Hpa II...
Regulation of 5-methyltetrahydrofolate synthesis.
Lumb, M, Bottiglieri, T, Deacon, R, Perry, J, Chanarin, I
After an intraperitoneal injection of 100 mumol of methionine to rats, there is rapid oxidation of the methyl group of hepatic 5-methyltetrahydrofolate to formate and CO2. Recovery of the...
Oxidation of 5-methyltetrahydrofolate in cobalamin-inactivated rats.
Lumb, M, Deacon, R, Perry, J, Chanarin, I
Rats were exposed to nitrous oxide, which inactivates cob(I)alamin (Cbl). As in air-breathing rats methionine administration led to the conversion of hepatic 5-methyltetrahydrofolate (MeH4 folate)...
In vivo oxidation of the methyl group of hepatic 5-methyltetrahydrofolate.
Lumb, M, Chanarin, I, Deacon, R, Perry, J
Methionine given parenterally to rats caused rapid disappearance of methyltetrahydrofolate from the liver and a corresponding rise in tetrahydrofolate and formyl-tetrahydrofolate concentrations. When...
Deacon, R, Perry, J, Lumb, M, Chanarin, I
N2O oxidizes and inactivates cob[I]alamin, and animals exposed in this way serve as models for cobalamin 'deficiency'. Such animals show a fall in activity of glycinamide ribotide transformylase and...
Folate polyglutamate synthetase activity in the cobalamin-inactivated rat.
Perry, J, Chanarin, I, Deacon, R, Lumb, M
Exposure to N2O inactivates cob[I]alamin and interferes with the activity of methionine synthetase, of which cob[I]alamin is a coenzyme. Less directly, it stops the formation of folate polyglutamate...
Lumb, M, Deacon, R, Perry, J, Chanarin, I, Minty, B, Halsey, M J, ...
Rats exposed to N20 show a decrease in liver folate to about 25% of the initial value after 10 days. There is a transient increase in the amount of 5-methylterrahydropteroylpolyglutamate in the first...
Role of sulphasalazine in the aetiology of folate deficiency in ulcerative colitis.
Swinson, C M, Perry, J, Lumb, M, Levi, A J
Only two (2.5%) of 80 outpatients with histologically proven ulcerative colitis had folate deficiency associated with anaemia or macrocytosis. Mean folate absorption, measured using micrograms/kg...
Deacon, Rosemary, Perry, Janet, Chanarin, I., Lumb, M.
The deoxyuridine (dU) suppression test assesses the capacity of marrow cells or activated lymphocytes to convert exogenous dU into thymidine. In addition, the effect of the added cobalamin or folate...