Detection of all single-base mismatches in solution by chemiluminescence.
Nelson, N C, Hammond, P W, Matsuda, E, Goud, A A, Becker, M M
A rapid in-solution method for the detection of all 12 single-base mismatches is described. The technique is based on the hybridization protection assay (HPA) format that utilizes oligonucleotide...
Mazumder, A, Majlessi, M, Becker, M M
We describe a high throughput microtiter-based assay to measure binding of oligodeoxyribonucleotides to nucleic acid targets. The assay utilizes oligodeoxyribonucleotide probes labeled with a highly...
Advantages of 2'-O-methyl oligoribonucleotide probes for detecting RNA targets.
Majlessi, M, Nelson, N C, Becker, M M
We have compared various kinetic and melting properties of oligoribonucleotide probes containing 2'-O-methylnucleotides or 2'-deoxynucleotides with regard to their use in assays for the detection of...
Boursier, P, Hanus, F J, Papen, H, Becker, M M, Russell, S A, Evans, H J
We have investigated the effect of added selenite on autotrophic growth and the time course of hydrogen oxidation derepression in Bradyrhizobium japonicum 122DES cultured in a medium purified to...
Selective visualization of gene structure with ultraviolet light.
The ability of the ultraviolet (UV) "footprinting" technique to detect chromatin has been investigated in vitro. Two basic types of chromatin, a phased nucleosome and a phased nucleosome containing a...
Becker, M M, Lesser, D, Kurpiewski, M, Baranger, A, Jen-Jacobson, L
The "UV footprinting" technique has been used to detect contacts between EcoRI endonuclease and its recognition sequence at single nucleotide resolution. Comparison of the UV-footprinting results to...
Genomic footprinting in mammalian cells with ultraviolet light.
Becker, M M, Wang, Z, Grossmann, G, Becherer, K A
A simple and accurate genomic primer extension method has been developed to detect ultraviolet footprinting patterns of regulatory protein-DNA interactions in mammalian genomic DNA. The technique can...
Detection of all single-base mismatches in solution by chemiluminescence.
Nelson, N C, Hammond, P W, Matsuda, E, Goud, A A, Becker, M M
A rapid in-solution method for the detection of all 12 single-base mismatches is described. The technique is based on the hybridization protection assay (HPA) format that utilizes oligonucleotide...
Mazumder, A, Majlessi, M, Becker, M M
We describe a high throughput microtiter-based assay to measure binding of oligodeoxyribonucleotides to nucleic acid targets. The assay utilizes oligodeoxyribonucleotide probes labeled with a highly...
Advantages of 2'-O-methyl oligoribonucleotide probes for detecting RNA targets.
Majlessi, M, Nelson, N C, Becker, M M
We have compared various kinetic and melting properties of oligoribonucleotide probes containing 2'-O-methylnucleotides or 2'-deoxynucleotides with regard to their use in assays for the detection of...
Boursier, P, Hanus, F J, Papen, H, Becker, M M, Russell, S A, Evans, H J
We have investigated the effect of added selenite on autotrophic growth and the time course of hydrogen oxidation derepression in Bradyrhizobium japonicum 122DES cultured in a medium purified to...
Selective visualization of gene structure with ultraviolet light.
The ability of the ultraviolet (UV) "footprinting" technique to detect chromatin has been investigated in vitro. Two basic types of chromatin, a phased nucleosome and a phased nucleosome containing a...
Becker, M M, Lesser, D, Kurpiewski, M, Baranger, A, Jen-Jacobson, L
The "UV footprinting" technique has been used to detect contacts between EcoRI endonuclease and its recognition sequence at single nucleotide resolution. Comparison of the UV-footprinting results to...
Genomic footprinting in mammalian cells with ultraviolet light.
Becker, M M, Wang, Z, Grossmann, G, Becherer, K A
A simple and accurate genomic primer extension method has been developed to detect ultraviolet footprinting patterns of regulatory protein-DNA interactions in mammalian genomic DNA. The technique can...