Okano, M., Hara, H., Muramatsu, M., Doi, K., Uetake, S., Takasu, Y., ...
We have successfully implemented the first simultaneous magneto-optical trapping (MOT) of lithium ($^6$Li) and ytterbium ($^{174}$Yb) atoms, towards production of ultracold polar molecules of LiYb....
Kase, S, Saito, W, Yokoi, M, Yoshida, K, Furudate, N, Muramatsu, M, ...
Background/aim: The mechanisms of the cellular origin and cell proliferation in the idiopathic epiretinal membrane (ERM) are unsolved. The aim of this study was to examine the expression of cell...
Kase, S, Saito, W, Yokoi, M, Yoshida, K, Furudate, N, Muramatsu, M, ...
Background/aim: The mechanisms of the cellular origin and cell proliferation in the idiopathic epiretinal membrane (ERM) are unsolved. The aim of this study was to examine the expression of cell...
Kase, S, Saito, W, Yokoi, M, Yoshida, K, Furudate, N, Muramatsu, M, ...
Background/aim: The mechanisms of the cellular origin and cell proliferation in the idiopathic epiretinal membrane (ERM) are unsolved. The aim of this study was to examine the expression of cell...
Self-assembled multilayers of titania nanoparticles and nanosheets with polyelectrolytes (2003)
Wang, ZS, Sasaki, T, Muramatsu, M, Ebina, Y, Tanaka, T, Wang, LZ, ...
Self-assembled multilayer films of titania nanoparticles and composite films of nanoparticles/nanosheets have been fabricated on various substrates using appropriate polyelectrolytes as counterions....
Self-assembled multilayers of titania nanoparticles and nanosheets with polyelectrolytes (2003)
Wang, ZS, Sasaki, T, Muramatsu, M, Ebina, Y, Tanaka, T, Wang, LZ, ...
Self-assembled multilayer films of titania nanoparticles and composite films of nanoparticles/nanosheets have been fabricated on various substrates using appropriate polyelectrolytes as counterions....
Mizuno, Y, Carninci, P, Okazaki, Y, Tateno, M, Kawai, J, Amanuma, H, ...
Imai, T, Masui, T, Ichinose, M, Nakanishi, H, Yanai, T, Masegi, T, ...
Itoh, M, Carninci, P, Nagaoka, S, Sasaki, N, Okazaki, Y, Ohsumi, T, ...
A new VNTR polymorphism at locus D1S340 (1993)
Honma, M, Mitani, K, Mizusawa, H, Hayashi, M, Sofuni, T, Muramatsu, M, ...
A new VNTR-type RFLP probe (lambda TM-18) on chromosome 1 (D1S157) (1992)
Honma, M, Mitani, K, Mizusawa, H, Sofuni, T, Muramatsu, M, Kominami, R
A new VNTR-type RFLP probe (ChdTC-114) on chromosome 20p (D20S72) (1992)
Honma, M, Mitani, K, Mizusawa, H, Sofuni, T, Muramatsu, M, Kominami, R
A TaqI polymorphism at human homeo box gene locus on chromosome 12 (1988)
Kamino, K, Miki, T, Takai, S, Yoshida, MC, Takahashi, N, Muramatsu, M
Organization of ribosomal RNA gene repeats of the mouse (1981)
Kominami, R., Urano, Y., Mishima, Y., Muramatsu, M.
The organization of the ribosomal RNA (rRNA) genes of the mouse was determined by Southern blot hybridization using cloned rDNA fragments as probes, which could encompass the entire spacer region...
Okazaki, Y, Hirose, K, Hirotsune, S, Okuizumi, H, Sasaki, N, Ohsumi, T, ...
We have developed a technique for isolating DNA markers tightly linked to a target region that is based on RLGS, named RLGS spot-bombing (RLGS-SB). RLGS-SB allows us to scan the genome of higher...
Trans-activation of glutathione transferase P gene during chemical hepatocarcinogenesis of the rat.
Morimura, S, Suzuki, T, Hochi, S, Yuki, A, Nomura, K, Kitagawa, T, ...
Glutathione transferase P (GST-P; glutathione transferase, EC 2.5.1.18) is known to be specifically expressed at high levels in precancerous lesions and in hepatocellular carcinomas from a very early...
Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
Inoue, S, Orimo, A, Hosoi, T, Kondo, S, Toyoshima, H, Kondo, T, ...
Estrogen receptor (ER)-binding fragments were isolated from human genomic DNA by using a recombinant ER protein. Using one of these fragments as a probe, we have identified an estrogen-responsive...
The nuclear protooncogenes c-jun and c-fos as regulators of DNA replication.
Murakami, Y, Satake, M, Yamaguchi-Iwai, Y, Sakai, M, Muramatsu, M, Ito, Y
Polyomavirus (Py) DNA replication is regulated by its enhancer, which contains an AP1 site, c-Jun and c-Fos, the products of nuclear protooncogenes c-jun and c-fos, form the heterodimeric...
Yamaguchi-Iwai, Y, Satake, M, Murakami, Y, Sakai, M, Muramatsu, M, Ito, Y
The activated c-Ha-ras oncogene induced AP1-site DNA-binding activity in F9 cells. This induction appeared to be due, at least in part, to the induction of c-jun transcription. Both activated...
Itoh, M, Carninci, P, Nagaoka, S, Sasaki, N, Okazaki, Y, Ohsumi, T, ...
We developed a new simple high-throughput plasmid DNA extraction procedure, based on a modified alkaline lysis method, using only one 96-well microtiter glassfilter plate. In this method, cell...
Ogawa, S, Inoue, S, Watanabe, T, Orimo, A, Hosoi, T, Ouchi, Y, ...
We have identified and characterized a novel human estrogen receptor (ER) beta isoform, ERbetacx, which is truncated at the C-terminal region but has an extra 26 amino acids due to alternative...
Mizuno, Y, Carninci, P, Okazaki, Y, Tateno, M, Kawai, J, Amanuma, H, ...
We have developed a method for high-efficiency window separation of cDNA display by increasing the specificity of priming in reverse transcription. In the conventional method, two-base anchored...
Takahashi, T, Sawada, T, Ohmae, K, Terakado, N, Muramatsu, M, Seto, K, ...
The susceptibility of 258 isolates of Erysipelothrix rhusiopathiae from slaughtered pigs affected with chronic erysipelas in Japan to antimicrobial agents was determined. A total of 111 (43.0%)...
Detection by PCR of wild-type canine parvovirus which contaminates dog vaccines.
Senda, M, Parrish, C R, Harasawa, R, Gamoh, K, Muramatsu, M, Hirayama, N, ...
A method for detecting wild-type canine parvovirus (CPV) strains which contaminate vaccines for dogs has been developed by PCR. PCR primers which distinguish vaccine strains from the most common,...
Kuromitsu, J, Yamashita, H, Kataoka, H, Takahara, T, Muramatsu, M, Sekine, T, ...
To understand the effect of trisomic chromosome 21 on the cause of Down syndrome (DS), DNA methylation in the CpG island, which regulates the expression of adjacent genes, was investigated with the...
Takahashi, T, Sawada, T, Muramatsu, M, Tamura, Y, Fujisawa, T, Benno, Y, ...
Erysipelothrix rhusiopathiae was isolated from tonsils of 63 (10.5%) of 600 apparently healthy slaughter pigs in the Kanto area of Japan in February and July 1984. The isolation rate was...
Mechanism of plasma clotting by Erysipelothrix rhusiopathiae.
Takahashi, T, Takahashi, I, Tamura, Y, Sawada, T, Yoshida, T, Suzuki, S, ...
Experiments were performed to determine the mechanism by which Erysipelothrix rhusiopathiae clots plasma. Detection of plasma-clotting activity in four strains of E. rhusiopathiae was carried out by...
Muramatsu, M, Yan, J, Eto, K, Tomoda, T, Yamada, R, Arai, K
Receptor-type serine/threonine kinases (RSKs) have been organized into two distinct classes known as types I and II on the basis of sequence similarity. However, experiments have shown ligand...
Erythroid differentiation factor is encoded by the same mRNA as that of the inhibin beta A chain.
Murata, M, Eto, Y, Shibai, H, Sakai, M, Muramatsu, M
We have isolated a protein that exhibits a potent differentiation-inducing activity toward mouse Friend erythroleukemia (MEL) cells and human K-562 cells. The protein, designated erythroid...
Sakai, M, Okuda, A, Muramatsu, M
We have analyzed the cis-acting regulatory DNA elements of the placental rat glutathione S-alkyltransferase (GST-P) gene. Various regions of the 5' flanking sequence were fused with a bacterial...
Yamamoto, M, Igarashi, T, Muramatsu, M, Fukagawa, M, Motokura, T, Ogata, E
To examine the effects of serum calcium concentrations on PTH biosynthesis, rats were made hyper- (serum total calcium, approximately 3.5 mM) or hypocalcemic (approximately 1.25 mM) and steady-state...
Chiba, H, Muramatsu, M, Nomoto, A, Kato, H
A set of genes (SWI1, SWI2/SNF2, SWI3, SNF5 and SNF6) in Saccharomyces cerevisiae are required for transcription of a variety of yeast genes. It was recently reported that the mammalian...
Extracts obtained from mouse cells growth arrested at stationary phase or under serum starvation exhibit no specific rDNA transcription activity. Experiments with mixed transcriptionally active and...
Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome.
Sumita, K, Makino, Y, Katoh, K, Kishimoto, T, Muramatsu, M, Mikoshiba, K, ...
Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse.
Kominami, R, Mishima, Y, Urano, Y, Sakai, M, Muramatsu, M
A Eco RI 6.6 kb DNA fragment containing the 3'-end of 28S ribosomal RNA gene of the mouse was detected by Southern blot hybridization, and cloned in a lambda-phage vector. The site of transcription...
Iwamura, Y, Sakai, M, Muramatsu, M
Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive...
Nabeshima, Y, Fujii-Kuriyama, Y, Muramatsu, M, Ogata, K
We report here the molecular cloning and sequence analysis of DNAs complementary to mRNAs for myosin alkali light chain of chicken embryo and adult leg skeletal muscle. pSMA2-1 contained an 818...
Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA.
Suguoka, Y, Kano, T, Okuda, A, Sakai, M, Kitagawa, T, Muramatsu, M
A cDNA library prepared from poly(A)+ RNA of 2-acetylaminofluorene (AAF) induced rat hepatocellular carcinoma was screened by synthetic DNA probes deduced from a partial amino acid sequence of...
Mishima, Y, Financsek, I, Kominami, R, Muramatsu, M
Mouse and human cell extracts (S100) can support an accurate and efficient transcription initiation on homologous ribosomal RNA gene (rDNA) templates. The cell extracts were fractionated with the aid...
In vitro splicing of SV40 late mRNA in isolated nuclei from CV-1 cells.
Hamada, H, Igarashi, T, Muramatsu, M
An in vitro splicing system utilizing isolated nuclei of SV40 infected cells has been developed. Nuclei were isolated from CV-1 cells at a late stage of SV40 infection after a pulse-labeling with...
3'-terminal processing of ribosomal RNA precursors in mammalian cells.
Hamada, H, Kominami, R, Muramatsu, M
The 3'-terminal structures of ribosomal 28S RNA and its precursors from rat and mouse were analyzed by means of periodate oxidation followed by reduction with 3H-borohydride. 3'-terminal labeled...
Organization of ribosomal RNA gene repeats of the mouse.
Kominami, R, Urano, Y, Mishima, Y, Muramatsu, M
The organization of the ribosomal RNA (rRNA) genes of the mouse was determined by Southern blot hybridization using cloned rDNA fragments as probes, which could encompass the entire spacer region...
In vitro transcription of a cloned mouse ribosomal RNA gene.
Mishima, Y, Yamamoto, O, Kominami, R, Muramatsu, M
An in vitro transcription system which utilizes cloned mouse ribosomal RNA gene (rDNA) fragments and a mouse cell extract has been developed. RNA polymerases I is apparently responsible for this...
Urano, Y, Kominami, R, Mishima, Y, Muramatsu, M
Approximately one kilobase pairs surrounding and upstream the transcription initiation site of a cloned ribosomal DNA (rDNA) of the mouse were sequenced. The putative transcription initiation site...
Tamura, T, Sumita, K, Fujino, I, Aoyama, A, Horikoshi, M, Hoffmann, A, ...
A complementary DNA (cDNA) encoding a mouse TFIID (mIID) was isolated from mouse brain cDNA libraries. The 316 amino acid sequence deduced from cDNA sequences revealed the presence of an...
Isolation of estrogen receptor-binding sites in human genomic DNA.
Inoue, S, Kondo, S, Hashimoto, M, Kondo, T, Muramatsu, M
Total genomic DNA digested by restriction enzymes was mixed with the DNA-binding domain of the estrogen receptor (ER-DBD) that was expressed in Escherichia coli and the fragments that bound to it...
Imagawa, M, Miyamoto, A, Shirakawa, M, Hamada, H, Muramatsu, M
POU-specific and POU-homeo domains of Oct3 were produced in Echerichia coli for characterization of DNA binding to the octamer sequence. POU domain protein including A, B and H domains could bind to...
Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF.
Hisatake, K, Nishimura, T, Maeda, Y, Hanada, K, Song, C Z, Muramatsu, M
The gene and protein structure of the mouse UBF (mUBF), a transcription factor for mouse ribosomal RNA gene, have been determined by cDNA and genomic clones. The unique mUBF gene consists of 21 exons...
Silencer binding proteins function on multiple cis-elements in the glutathione transferase P gene.
Imagawa, M, Osada, S, Okuda, A, Muramatsu, M
The glutathione transferase P (GST-P) gene is specifically expressed during chemical hepatocarcinogenesis of the rat, whereas mRNA of this gene is virtually undetectable in normal liver. We have...
The dyad palindromic glutathione transferase P enhancer binds multiple factors including AP1.
Diccianni, M B, Imagawa, M, Muramatsu, M
Glutathione Transferase P (GST-P) gene expression is dominantly regulated by an upstream enhancer (GPEI) consisting of a dyad of palindromically oriented imperfect TPA...
The regulation of the murine Hox-2.5 gene expression during cell differentiation.
Kondo, T, Takahashi, N, Muramatsu, M
The mouse Hox-2.5 gene containing a Drosophila Antennapedia-type homeobox sequence is expressed in a spatially and temporally restricted manner during embryogenesis. We found that the mouse embryonal...
A TaqI polymorphism at human homeo box gene locus on chromosome 12.
Kamino, K, Miki, T, Takai, S, Yoshida, M C, Takahashi, N, Muramatsu, M
Transcription termination and RNA processing in the 3'-end spacer of mouse ribosomal RNA genes.
Miwa, T, Kominami, R, Yoshikura, H, Sudo, K, Muramatsu, M
The 3' termini of ribosomal RNA precursors from mouse FM3A cultured cells are mapped to eight sites within 625 bp downstream from the 3' terminus of 28 S rRNA. Three additional sites are mapped in...
Molecular cloning and characterization of rat estrogen receptor cDNA.
Koike, S, Sakai, M, Muramatsu, M
A cDNA clone of rat uterus estrogen receptor (ER) has been isolated and sequenced. This clone contains a complete open reading frame encoding 600 amino acid residues which is 5 and 11 amino acids...
Kishimoto, T, Nagamine, M, Sasaki, T, Takakusa, N, Miwa, T, Kominami, R, ...
Point mutations are introduced into a mouse rDNA fragment containing the promoter region by a sodium bisulfite method and the mutants are tested for the ability of accurate transcription initiation...
Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.
The 5'-termini of nucleolar 45S, 32S and 28S RNA's were analyzed by means of thin layer chromatography and Dowex-1 colum chromatography. 45S RNA did not bear a triphosphate at the 5'-terminus, but...
Yamamoto, O, Takakusa, N, Mishima, Y, Kominami, R, Muramatsu, M
Sequences required for a faithful and efficient transcription of a cloned mouse ribosomal RNA gene (rDNA) are determined by testing a series of deletion mutants in an in vitro transcription system...
Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver.
Kawajiri, K, Gotoh, O, Sogawa, K, Tagashira, Y, Muramatsu, M, Fujii-Kuriyama, Y
We determined the coding nucleotide sequence of the mRNA for a 3-methylcholanthrene-inducible cytochrome P-450, P-450d, of rat liver by sequence analysis of cloned cDNAs. The predicted amino acid...
Fujii-Kuriyama, Y, Mizukami, Y, Kawajiri, K, Sogawa, K, Muramatsu, M
We determined the coding nucleotide sequence of phenobarbital-inducible cytochrome P-450 mRNA of the rat by analysis of three cloned cDNAs and by primer extension methods. The deduced amino acid...
Financsek, I, Mizumoto, K, Mishima, Y, Muramatsu, M
The transcription initiation site of the human ribosomal RNA gene (rDNA) was located by using the single-strand specific nuclease protection method and by determining the first nucleotide of the in...
Molecular cloning of human interferon cDNA.
Taniguchi, T, Fujii-Kuriyama, Y, Muramatsu, M
A hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S. & Sudo, T. (1979)...
Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.
Safrany, G, Tanaka, N, Kishimoto, T, Ishikawa, Y, Kato, H, Kominami, R, ...
Mammalian ribosomal DNA (rDNA) transcription has a certain species specificity such that, both in vivo and in vitro, human rDNA cannot be transcribed by mouse machinery and vice versa. This is due to...
Muramatsu, M, Kaibuchi, K, Arai, K
We constructed mutant protein kinase C (PKC) cDNAs which expressed PKC activity in vivo in the absence of phorbol ester activation. A hybrid PKC gene, PKAC, was constructed by substituting the coding...
Nagamine, M, Kishimoto, T, Aono, J, Kato, H, Kominami, R, Muramatsu, M
We compared the ability of various deletion and substitution mutants of the mouse rRNA gene promoter to bind essential factors required for accurate transcription initiation by RNA polymerase I....
Formation of the transcription initiation complex on mammalian rDNA.
Kato, H, Nagamine, M, Kominami, R, Muramatsu, M
Steps for the formation of transcription initiation complex on the human rRNA gene (rDNA) in vitro were analyzed with partially purified transcription factors and RNA polymerase I. The reaction...
Gene structure of a phenobarbital-inducible cytochrome P-450 in rat liver.
Mizukami, Y, Sogawa, K, Suwa, Y, Muramatsu, M, Fujii-Kuriyama, Y
The gene structure of a phenobarbital-inducible rat liver cytochrome P-50 was elucidated by sequence analysis of two isolated genomic clones. The total length of the gene was approximately 14...
Shimazaki, T, Okazawa, H, Fujii, H, Ikeda, M, Tamai, K, McKay, R D, ...
The Oct-3 gene is expressed in highly undifferentiated cells and is implicated in mammalian early embryogenesis. We have generated a series of hybrid cells between pluripotent embryonal carcinoma...
Hanada, K, Song, C Z, Yamamoto, K, Yano, K, Maeda, Y, Yamaguchi, K, ...
Mouse RNA polymerase I (Pol I) has, besides its 11 bona fide subunits, three polymerase associated factors, termed PAF53, 51 and 49 with respect to the size of each molecule. In order to analyze the...
Okazawa, H, Okamoto, K, Ishino, F, Ishino-Kaneko, T, Takeda, S, Toyoda, Y, ...
Oct3 is an embryonic octamer-binding transcription factor, whose expression is rapidly repressed by retinoic acid (RA). In this report, we have determined the transcriptional control region of the...
Multimerization of the mouse TATA-binding protein (TBP) driven by its C-terminal conserved domain.
Kato, K, Makino, Y, Kishimoto, T, Yamauchi, J, Kato, S, Muramatsu, M, ...
The conformational states of the mouse TATA-binding protein (TBP) in solution were studied. A histidine tag and a factor Xa recognition site-carrying mouse TBP was expressed in E. coli, highly...
Maeda, Y, Hisatake, K, Kondo, T, Hanada, K, Song, C Z, Nishimura, T, ...
RNA polymerase I requires at least two nucleolar transcription factors, UBF and SL-1, for ribosomal RNA gene (rDNA) transcription. UBF requires SL-1 for the formation of a stable initiation complex...
Nomenclature for human glutathione transferases.
Mannervik, B, Awasthi, Y C, Board, P G, Hayes, J D, Di Ilio, C, Ketterer, B, ...
Black, S M, Beggs, J D, Hayes, J D, Bartoszek, A, Muramatsu, M, Sakai, M, ...
Adaptation and resistance to chemicals in the environment is a critical part of the evolutionary process. As a result, a wide variety of defence systems that protect cells against chemical insult...
Okuda, A, Fukushima, A, Nishimoto, M, Orimo, A, Yamagishi, T, Nabeshima, Y, ...
We have obtained a novel transcriptional cofactor, termed undifferentiated embryonic cell transcription factor 1 (UTF1), from F9 embryonic carcinoma (EC) cells. This protein is expressed in EC and...
Okuda, A, Imagawa, M, Sakai, M, Muramatsu, M
We have recently identified an enhancer, termed GPEI, in the 5'-flanking region of the rat glutathione transferase P gene, that is composed of two imperfect TPA (phorbol 12-O-tetradecanoate...
A Genetic Linkage Map of the Mouse Using Restriction Landmark Genomic Scanning (Rlgs)
Hayashizaki, Y., Hirotsune, S., Okazaki, Y., Shibata, H., Akasako, A., Muramatsu, M., ...
We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled...
Okazaki, Y, Hirose, K, Hirotsune, S, Okuizumi, H, Sasaki, N, Ohsumi, T, ...
We have developed a technique for isolating DNA markers tightly linked to a target region that is based on RLGS, named RLGS spot-bombing (RLGS-SB). RLGS-SB allows us to scan the genome of higher...
Trans-activation of glutathione transferase P gene during chemical hepatocarcinogenesis of the rat.
Morimura, S, Suzuki, T, Hochi, S, Yuki, A, Nomura, K, Kitagawa, T, ...
Glutathione transferase P (GST-P; glutathione transferase, EC 2.5.1.18) is known to be specifically expressed at high levels in precancerous lesions and in hepatocellular carcinomas from a very early...
Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
Inoue, S, Orimo, A, Hosoi, T, Kondo, S, Toyoshima, H, Kondo, T, ...
Estrogen receptor (ER)-binding fragments were isolated from human genomic DNA by using a recombinant ER protein. Using one of these fragments as a probe, we have identified an estrogen-responsive...
The nuclear protooncogenes c-jun and c-fos as regulators of DNA replication.
Murakami, Y, Satake, M, Yamaguchi-Iwai, Y, Sakai, M, Muramatsu, M, Ito, Y
Polyomavirus (Py) DNA replication is regulated by its enhancer, which contains an AP1 site, c-Jun and c-Fos, the products of nuclear protooncogenes c-jun and c-fos, form the heterodimeric...
Yamaguchi-Iwai, Y, Satake, M, Murakami, Y, Sakai, M, Muramatsu, M, Ito, Y
The activated c-Ha-ras oncogene induced AP1-site DNA-binding activity in F9 cells. This induction appeared to be due, at least in part, to the induction of c-jun transcription. Both activated...
Itoh, M, Carninci, P, Nagaoka, S, Sasaki, N, Okazaki, Y, Ohsumi, T, ...
We developed a new simple high-throughput plasmid DNA extraction procedure, based on a modified alkaline lysis method, using only one 96-well microtiter glassfilter plate. In this method, cell...
Ogawa, S, Inoue, S, Watanabe, T, Orimo, A, Hosoi, T, Ouchi, Y, ...
We have identified and characterized a novel human estrogen receptor (ER) beta isoform, ERbetacx, which is truncated at the C-terminal region but has an extra 26 amino acids due to alternative...
Mizuno, Y, Carninci, P, Okazaki, Y, Tateno, M, Kawai, J, Amanuma, H, ...
We have developed a method for high-efficiency window separation of cDNA display by increasing the specificity of priming in reverse transcription. In the conventional method, two-base anchored...
Takahashi, T, Sawada, T, Ohmae, K, Terakado, N, Muramatsu, M, Seto, K, ...
The susceptibility of 258 isolates of Erysipelothrix rhusiopathiae from slaughtered pigs affected with chronic erysipelas in Japan to antimicrobial agents was determined. A total of 111 (43.0%)...
Detection by PCR of wild-type canine parvovirus which contaminates dog vaccines.
Senda, M, Parrish, C R, Harasawa, R, Gamoh, K, Muramatsu, M, Hirayama, N, ...
A method for detecting wild-type canine parvovirus (CPV) strains which contaminate vaccines for dogs has been developed by PCR. PCR primers which distinguish vaccine strains from the most common,...
Kuromitsu, J, Yamashita, H, Kataoka, H, Takahara, T, Muramatsu, M, Sekine, T, ...
To understand the effect of trisomic chromosome 21 on the cause of Down syndrome (DS), DNA methylation in the CpG island, which regulates the expression of adjacent genes, was investigated with the...
Takahashi, T, Sawada, T, Muramatsu, M, Tamura, Y, Fujisawa, T, Benno, Y, ...
Erysipelothrix rhusiopathiae was isolated from tonsils of 63 (10.5%) of 600 apparently healthy slaughter pigs in the Kanto area of Japan in February and July 1984. The isolation rate was...
Mechanism of plasma clotting by Erysipelothrix rhusiopathiae.
Takahashi, T, Takahashi, I, Tamura, Y, Sawada, T, Yoshida, T, Suzuki, S, ...
Experiments were performed to determine the mechanism by which Erysipelothrix rhusiopathiae clots plasma. Detection of plasma-clotting activity in four strains of E. rhusiopathiae was carried out by...
Muramatsu, M, Yan, J, Eto, K, Tomoda, T, Yamada, R, Arai, K
Receptor-type serine/threonine kinases (RSKs) have been organized into two distinct classes known as types I and II on the basis of sequence similarity. However, experiments have shown ligand...
Erythroid differentiation factor is encoded by the same mRNA as that of the inhibin beta A chain.
Murata, M, Eto, Y, Shibai, H, Sakai, M, Muramatsu, M
We have isolated a protein that exhibits a potent differentiation-inducing activity toward mouse Friend erythroleukemia (MEL) cells and human K-562 cells. The protein, designated erythroid...
Sakai, M, Okuda, A, Muramatsu, M
We have analyzed the cis-acting regulatory DNA elements of the placental rat glutathione S-alkyltransferase (GST-P) gene. Various regions of the 5' flanking sequence were fused with a bacterial...
Yamamoto, M, Igarashi, T, Muramatsu, M, Fukagawa, M, Motokura, T, Ogata, E
To examine the effects of serum calcium concentrations on PTH biosynthesis, rats were made hyper- (serum total calcium, approximately 3.5 mM) or hypocalcemic (approximately 1.25 mM) and steady-state...
Chiba, H, Muramatsu, M, Nomoto, A, Kato, H
A set of genes (SWI1, SWI2/SNF2, SWI3, SNF5 and SNF6) in Saccharomyces cerevisiae are required for transcription of a variety of yeast genes. It was recently reported that the mammalian...
Extracts obtained from mouse cells growth arrested at stationary phase or under serum starvation exhibit no specific rDNA transcription activity. Experiments with mixed transcriptionally active and...
Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome.
Sumita, K, Makino, Y, Katoh, K, Kishimoto, T, Muramatsu, M, Mikoshiba, K, ...
Cloning and determination of the transcription termination site of ribosomal RNA gene of the mouse.
Kominami, R, Mishima, Y, Urano, Y, Sakai, M, Muramatsu, M
A Eco RI 6.6 kb DNA fragment containing the 3'-end of 28S ribosomal RNA gene of the mouse was detected by Southern blot hybridization, and cloned in a lambda-phage vector. The site of transcription...
Iwamura, Y, Sakai, M, Muramatsu, M
Three clones of non-repetitive sequences and six clones containing repetitive sequences were obtained from micronuclear DNA of Tetrahymena thermophila. All the non-repetitive and three repetitive...
Nabeshima, Y, Fujii-Kuriyama, Y, Muramatsu, M, Ogata, K
We report here the molecular cloning and sequence analysis of DNAs complementary to mRNAs for myosin alkali light chain of chicken embryo and adult leg skeletal muscle. pSMA2-1 contained an 818...
Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA.
Suguoka, Y, Kano, T, Okuda, A, Sakai, M, Kitagawa, T, Muramatsu, M
A cDNA library prepared from poly(A)+ RNA of 2-acetylaminofluorene (AAF) induced rat hepatocellular carcinoma was screened by synthetic DNA probes deduced from a partial amino acid sequence of...
Mishima, Y, Financsek, I, Kominami, R, Muramatsu, M
Mouse and human cell extracts (S100) can support an accurate and efficient transcription initiation on homologous ribosomal RNA gene (rDNA) templates. The cell extracts were fractionated with the aid...
In vitro splicing of SV40 late mRNA in isolated nuclei from CV-1 cells.
Hamada, H, Igarashi, T, Muramatsu, M
An in vitro splicing system utilizing isolated nuclei of SV40 infected cells has been developed. Nuclei were isolated from CV-1 cells at a late stage of SV40 infection after a pulse-labeling with...
3'-terminal processing of ribosomal RNA precursors in mammalian cells.
Hamada, H, Kominami, R, Muramatsu, M
The 3'-terminal structures of ribosomal 28S RNA and its precursors from rat and mouse were analyzed by means of periodate oxidation followed by reduction with 3H-borohydride. 3'-terminal labeled...
Organization of ribosomal RNA gene repeats of the mouse.
Kominami, R, Urano, Y, Mishima, Y, Muramatsu, M
The organization of the ribosomal RNA (rRNA) genes of the mouse was determined by Southern blot hybridization using cloned rDNA fragments as probes, which could encompass the entire spacer region...
In vitro transcription of a cloned mouse ribosomal RNA gene.
Mishima, Y, Yamamoto, O, Kominami, R, Muramatsu, M
An in vitro transcription system which utilizes cloned mouse ribosomal RNA gene (rDNA) fragments and a mouse cell extract has been developed. RNA polymerases I is apparently responsible for this...
Urano, Y, Kominami, R, Mishima, Y, Muramatsu, M
Approximately one kilobase pairs surrounding and upstream the transcription initiation site of a cloned ribosomal DNA (rDNA) of the mouse were sequenced. The putative transcription initiation site...
Tamura, T, Sumita, K, Fujino, I, Aoyama, A, Horikoshi, M, Hoffmann, A, ...
A complementary DNA (cDNA) encoding a mouse TFIID (mIID) was isolated from mouse brain cDNA libraries. The 316 amino acid sequence deduced from cDNA sequences revealed the presence of an...
Isolation of estrogen receptor-binding sites in human genomic DNA.
Inoue, S, Kondo, S, Hashimoto, M, Kondo, T, Muramatsu, M
Total genomic DNA digested by restriction enzymes was mixed with the DNA-binding domain of the estrogen receptor (ER-DBD) that was expressed in Escherichia coli and the fragments that bound to it...
Imagawa, M, Miyamoto, A, Shirakawa, M, Hamada, H, Muramatsu, M
POU-specific and POU-homeo domains of Oct3 were produced in Echerichia coli for characterization of DNA binding to the octamer sequence. POU domain protein including A, B and H domains could bind to...
Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF.
Hisatake, K, Nishimura, T, Maeda, Y, Hanada, K, Song, C Z, Muramatsu, M
The gene and protein structure of the mouse UBF (mUBF), a transcription factor for mouse ribosomal RNA gene, have been determined by cDNA and genomic clones. The unique mUBF gene consists of 21 exons...
Silencer binding proteins function on multiple cis-elements in the glutathione transferase P gene.
Imagawa, M, Osada, S, Okuda, A, Muramatsu, M
The glutathione transferase P (GST-P) gene is specifically expressed during chemical hepatocarcinogenesis of the rat, whereas mRNA of this gene is virtually undetectable in normal liver. We have...
The dyad palindromic glutathione transferase P enhancer binds multiple factors including AP1.
Diccianni, M B, Imagawa, M, Muramatsu, M
Glutathione Transferase P (GST-P) gene expression is dominantly regulated by an upstream enhancer (GPEI) consisting of a dyad of palindromically oriented imperfect TPA...
The regulation of the murine Hox-2.5 gene expression during cell differentiation.
Kondo, T, Takahashi, N, Muramatsu, M
The mouse Hox-2.5 gene containing a Drosophila Antennapedia-type homeobox sequence is expressed in a spatially and temporally restricted manner during embryogenesis. We found that the mouse embryonal...
A TaqI polymorphism at human homeo box gene locus on chromosome 12.
Kamino, K, Miki, T, Takai, S, Yoshida, M C, Takahashi, N, Muramatsu, M
Transcription termination and RNA processing in the 3'-end spacer of mouse ribosomal RNA genes.
Miwa, T, Kominami, R, Yoshikura, H, Sudo, K, Muramatsu, M
The 3' termini of ribosomal RNA precursors from mouse FM3A cultured cells are mapped to eight sites within 625 bp downstream from the 3' terminus of 28 S rRNA. Three additional sites are mapped in...
Molecular cloning and characterization of rat estrogen receptor cDNA.
Koike, S, Sakai, M, Muramatsu, M
A cDNA clone of rat uterus estrogen receptor (ER) has been isolated and sequenced. This clone contains a complete open reading frame encoding 600 amino acid residues which is 5 and 11 amino acids...
Kishimoto, T, Nagamine, M, Sasaki, T, Takakusa, N, Miwa, T, Kominami, R, ...
Point mutations are introduced into a mouse rDNA fragment containing the promoter region by a sodium bisulfite method and the mutants are tested for the ability of accurate transcription initiation...
Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.
The 5'-termini of nucleolar 45S, 32S and 28S RNA's were analyzed by means of thin layer chromatography and Dowex-1 colum chromatography. 45S RNA did not bear a triphosphate at the 5'-terminus, but...
Yamamoto, O, Takakusa, N, Mishima, Y, Kominami, R, Muramatsu, M
Sequences required for a faithful and efficient transcription of a cloned mouse ribosomal RNA gene (rDNA) are determined by testing a series of deletion mutants in an in vitro transcription system...
Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver.
Kawajiri, K, Gotoh, O, Sogawa, K, Tagashira, Y, Muramatsu, M, Fujii-Kuriyama, Y
We determined the coding nucleotide sequence of the mRNA for a 3-methylcholanthrene-inducible cytochrome P-450, P-450d, of rat liver by sequence analysis of cloned cDNAs. The predicted amino acid...
Fujii-Kuriyama, Y, Mizukami, Y, Kawajiri, K, Sogawa, K, Muramatsu, M
We determined the coding nucleotide sequence of phenobarbital-inducible cytochrome P-450 mRNA of the rat by analysis of three cloned cDNAs and by primer extension methods. The deduced amino acid...
Financsek, I, Mizumoto, K, Mishima, Y, Muramatsu, M
The transcription initiation site of the human ribosomal RNA gene (rDNA) was located by using the single-strand specific nuclease protection method and by determining the first nucleotide of the in...
Molecular cloning of human interferon cDNA.
Taniguchi, T, Fujii-Kuriyama, Y, Muramatsu, M
A hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S. & Sudo, T. (1979)...
Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.
Safrany, G, Tanaka, N, Kishimoto, T, Ishikawa, Y, Kato, H, Kominami, R, ...
Mammalian ribosomal DNA (rDNA) transcription has a certain species specificity such that, both in vivo and in vitro, human rDNA cannot be transcribed by mouse machinery and vice versa. This is due to...
Muramatsu, M, Kaibuchi, K, Arai, K
We constructed mutant protein kinase C (PKC) cDNAs which expressed PKC activity in vivo in the absence of phorbol ester activation. A hybrid PKC gene, PKAC, was constructed by substituting the coding...
Nagamine, M, Kishimoto, T, Aono, J, Kato, H, Kominami, R, Muramatsu, M
We compared the ability of various deletion and substitution mutants of the mouse rRNA gene promoter to bind essential factors required for accurate transcription initiation by RNA polymerase I....
Formation of the transcription initiation complex on mammalian rDNA.
Kato, H, Nagamine, M, Kominami, R, Muramatsu, M
Steps for the formation of transcription initiation complex on the human rRNA gene (rDNA) in vitro were analyzed with partially purified transcription factors and RNA polymerase I. The reaction...
Gene structure of a phenobarbital-inducible cytochrome P-450 in rat liver.
Mizukami, Y, Sogawa, K, Suwa, Y, Muramatsu, M, Fujii-Kuriyama, Y
The gene structure of a phenobarbital-inducible rat liver cytochrome P-50 was elucidated by sequence analysis of two isolated genomic clones. The total length of the gene was approximately 14...
Shimazaki, T, Okazawa, H, Fujii, H, Ikeda, M, Tamai, K, McKay, R D, ...
The Oct-3 gene is expressed in highly undifferentiated cells and is implicated in mammalian early embryogenesis. We have generated a series of hybrid cells between pluripotent embryonal carcinoma...
Hanada, K, Song, C Z, Yamamoto, K, Yano, K, Maeda, Y, Yamaguchi, K, ...
Mouse RNA polymerase I (Pol I) has, besides its 11 bona fide subunits, three polymerase associated factors, termed PAF53, 51 and 49 with respect to the size of each molecule. In order to analyze the...
Okazawa, H, Okamoto, K, Ishino, F, Ishino-Kaneko, T, Takeda, S, Toyoda, Y, ...
Oct3 is an embryonic octamer-binding transcription factor, whose expression is rapidly repressed by retinoic acid (RA). In this report, we have determined the transcriptional control region of the...
Multimerization of the mouse TATA-binding protein (TBP) driven by its C-terminal conserved domain.
Kato, K, Makino, Y, Kishimoto, T, Yamauchi, J, Kato, S, Muramatsu, M, ...
The conformational states of the mouse TATA-binding protein (TBP) in solution were studied. A histidine tag and a factor Xa recognition site-carrying mouse TBP was expressed in E. coli, highly...
Okuda, A, Imagawa, M, Sakai, M, Muramatsu, M
We have recently identified an enhancer, termed GPEI, in the 5'-flanking region of the rat glutathione transferase P gene, that is composed of two imperfect TPA (phorbol 12-O-tetradecanoate...
Maeda, Y, Hisatake, K, Kondo, T, Hanada, K, Song, C Z, Nishimura, T, ...
RNA polymerase I requires at least two nucleolar transcription factors, UBF and SL-1, for ribosomal RNA gene (rDNA) transcription. UBF requires SL-1 for the formation of a stable initiation complex...
Nomenclature for human glutathione transferases.
Mannervik, B, Awasthi, Y C, Board, P G, Hayes, J D, Di Ilio, C, Ketterer, B, ...
Black, S M, Beggs, J D, Hayes, J D, Bartoszek, A, Muramatsu, M, Sakai, M, ...
Adaptation and resistance to chemicals in the environment is a critical part of the evolutionary process. As a result, a wide variety of defence systems that protect cells against chemical insult...
Okuda, A, Fukushima, A, Nishimoto, M, Orimo, A, Yamagishi, T, Nabeshima, Y, ...
We have obtained a novel transcriptional cofactor, termed undifferentiated embryonic cell transcription factor 1 (UTF1), from F9 embryonic carcinoma (EC) cells. This protein is expressed in EC and...
A Genetic Linkage Map of the Mouse Using Restriction Landmark Genomic Scanning (Rlgs)
Hayashizaki, Y., Hirotsune, S., Okazaki, Y., Shibata, H., Akasako, A., Muramatsu, M., ...
We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled...
Suzuki, H, Toyoda, S, Muramatsu, M, Shimizu, T, Kojima, T, Taki, W
Objective: To determine the incidence and clinical characteristics of spontaneous haemorrhage into metastatic brain tumours after radiosurgery.
The Interaction between Steroid Hormones and Lipid Monolayers on Water
Gershfeld, N. L., Muramatsu, M.
The interaction of progesterone, testosterone, androsterone, and etiocholanolone with insoluble lipid films (cholesterol and saturated hydrocarbons containing either alcohol, ester, acetamide,...
Matsuura, S., Morimoto, T., Tashiro, Y., Higashinakagawa, T., Muramatsu, M.
Sucrose density gradient analyses of pH 5.5 and pH 7.4 extracts from rat liver nucleoli revealed the presence of two broad peaks of approximately 60S and 80S, and 60S and 80–100S, respectively....
Yokoi, M, Yamagishi, S-i, Takeuchi, M, Ohgami, K, Okamoto, T, Saito, W, ...
Background/aims: Advanced glycation end product (AGE) induces vascular endothelial growth factor (VEGF) expression in cell culture and animal models, being considered to be involved in development of...
Expression of glutamine synthetase and cell proliferation in human idiopathic epiretinal membrane
Kase, S, Saito, W, Yokoi, M, Yoshida, K, Furudate, N, Muramatsu, M, ...
Suzuki, Y., Aoyagi, Y., Muramatsu, M., Sekine, C., Isemura, M., Ichida, F.
Monoclonal antibodies 18H4 and 19F12 against alpha-foetoprotein (AFP) were examined by enzyme immunoassay for binding to two forms of AFP that were separated on the basis of the reactivity with...
EBAG9/RCAS1 in human breast carcinoma: a possible factor in endocrine–immune interactions
Suzuki, T, Inoue, S, Kawabata, W, Akahira, J, Moriya, T, Tsuchiya, F, ...
EBAG9 has been recently identified as an oestrogen responsive gene in MCF-7 human breast carcinoma cells. EBAG9 is identical to RCAS1, a cancer cell surface antigen possibly involved in immune...