The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signals.
Tinland, B, Koukolíková-Nicola, Z, Hall, M N, Hohn, B
Agrobacterium tumefaciens causes neoplastic growth in plants by transferring a piece of DNA, called T-DNA, into the nucleus of the plant cell. The virulence protein VirD2 of A. tumefaciens is tightly...
A yeast cyclophilin gene essential for lactate metabolism at high temperature.
Davis, E S, Becker, A, Heitman, J, Hall, M N, Brennan, M B
The cyclophilins are a family of ubiquitous eukaryotic proteins first identified by high affinity for cyclosporin A (CsA). The immunosuppressant and cytotoxic effects of CsA are thought to result...
Heitman, J, Movva, N R, Hiestand, P C, Hall, M N
FK 506 and cyclosporin A are potent immunosuppressive compounds that inhibit T-cell activation by interfering with signal transduction. In vitro, FK 506 binds and inhibits the activity of FK...
Homeodomain of yeast repressor alpha 2 contains a nuclear localization signal.
Hall, M N, Craik, C, Hiraoka, Y
The yeast repressor alpha 2 is shown, by analysis of deletion-bearing alpha 2-beta-galactosidase hybrid proteins, to have two structurally distinct nuclear localization signals. The cellular location...
Benson, S A, Hall, M N, Rasmussen, B A
The lamB701-708 signal sequence mutation reduces expression of LamB, an outer membrane protein of Escherichia coli. To investigate the possibility that synthesis and export of LamB are coupled, as...
Taylor, R K, Hall, M N, Enquist, L, Silhavy, T J
We report here on the cloning of a gene located within the ompB locus of E. coli K-12. This gene, designated ompR, resides on a 10.9-kilobase EcoRI fragment that we cloned, using a lambda vector and...
Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b.
Eleven independent insertion mutations were isolated that prevented expression of major outer membrane protein 1b. Seven of the mutations were Mucts insertions located at ombP. These ompB::Mucts...
TOR controls translation initiation and early G1 progression in yeast.
Barbet, N C, Schneider, U, Helliwell, S B, Stansfield, I, Tuite, M F, Hall, M N
Saccharomyces cerevisiae cells treated with the immunosuppressant rapamycin or depleted for the targets of rapamycin TOR1 and TOR2 arrest growth in the early G1 phase of the cell cycle. Loss of TOR...
Helliwell, S B, Wagner, P, Kunz, J, Deuter-Reinhard, M, Henriquez, R, Hall, M N
The Saccharomyces cerevisiae genes TOR1 and TOR2 were originally identified by mutations that confer resistance to the immunosuppressant rapamycin. TOR2 was previously shown to encode an essential...
The immunosuppressant FK506 inhibits amino acid import in Saccharomyces cerevisiae.
Heitman, J, Koller, A, Kunz, J, Henriquez, R, Schmidt, A, Movva, N R, ...
The immunosuppressants cyclosporin A, FK506, and rapamycin inhibit growth of unicellular eukaryotic microorganisms and also block activation of T lymphocytes from multicellular eukaryotes. In vitro,...
PIK1, an essential phosphatidylinositol 4-kinase associated with the yeast nucleus.
Garcia-Bustos, J F, Marini, F, Stevenson, I, Frei, C, Hall, M N
Transmission of mitogenic and developmental signals to intracellular targets is often mediated by inositol derivatives. Here we present the cloning and characterization of a gene from Saccharomyces...
Rapamycin blocks the phosphorylation of 4E-BP1 and inhibits cap-dependent initiation of translation.
Beretta, L, Gingras, A C, Svitkin, Y V, Hall, M N, Sonenberg, N
The immunosuppressant drug rapamycin blocks progression of the cell cycle at the G1 phase in mammalian cells and yeast. Here we show that rapamycin inhibits cap-dependent, but not cap-independent,...
Evidence for a coupling of synthesis and export of an outer membrane protein in Escherichia coli.
Hall, M N, Gabay, J, Schwartz, M
We describe a lesion, lamB701-708, affecting the hydrophilic portion of the lambda receptor signal sequence. The C to A transversion of the sixth codon of the signal sequence changes a positively...
Cell wall integrity modulates RHO1 activity via the exchange factor ROM2.
Bickle, M, Delley, P A, Schmidt, A, Hall, M N
The essential phosphatidylinositol kinase homologue TOR2 of Saccharomyces cerevisiae controls the actin cytoskeleton by activating a GTPase switch consisting of RHO1 (GTPase), ROM2 (GEF) and SAC7...
Schmidt, A, Beck, T, Koller, A, Kunz, J, Hall, M N
The Saccharomyces cerevisiae targets of rapamycin, TOR1 and TOR2, signal activation of cell growth in response to nutrient availability. Loss of TOR or rapamycin treatment causes yeast cells to...
Danaie, P, Altmann, M, Hall, M N, Trachsel, H, Helliwell, S B
The essential cap-binding protein (eIF4E) of Saccharomyces cerevisiae is encoded by the CDC33 (wild-type) gene, originally isolated as a mutant, cdc33-1, which arrests growth in the G1 phase of the...
Schmidt, A, Hall, M N, Koller, A
The macrocyclic lactone FK506 exerts immunosuppressive effects on T lymphocytes by interfering with signal transduction leading to T-cell activation and also inhibits the growth of eukaryotic...
Shaffer, H J, Hall, M N, Vander Bilt, J
OBJECTIVES: This study developed prevalence estimates of gambling-related disorders in the United States and Canada, identified differences in prevalence among population segments, and identified...
Koller, A., Heitman, J., Hall, M. N.
In meiosis I, homologous chromosomes pair, recombine and segregate to opposite poles. These events and subsequent meiosis II ensure that each of the four meiotic products has one complete set of...
TOR2 is part of two related signaling pathways coordinating cell growth in Saccharomyces cerevisiae.
Helliwell, S B, Howald, I, Barbet, N, Hall, M N
The Saccharomyces cerevisiae genes TOR1 and TOR2 encode phosphatidylinositol kinase homologs. TOR2 has two essential functions. One function overlaps with TOR1 and mediates protein synthesis and cell...
The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signals.
Tinland, B, Koukolíková-Nicola, Z, Hall, M N, Hohn, B
Agrobacterium tumefaciens causes neoplastic growth in plants by transferring a piece of DNA, called T-DNA, into the nucleus of the plant cell. The virulence protein VirD2 of A. tumefaciens is tightly...
A yeast cyclophilin gene essential for lactate metabolism at high temperature.
Davis, E S, Becker, A, Heitman, J, Hall, M N, Brennan, M B
The cyclophilins are a family of ubiquitous eukaryotic proteins first identified by high affinity for cyclosporin A (CsA). The immunosuppressant and cytotoxic effects of CsA are thought to result...
Heitman, J, Movva, N R, Hiestand, P C, Hall, M N
FK 506 and cyclosporin A are potent immunosuppressive compounds that inhibit T-cell activation by interfering with signal transduction. In vitro, FK 506 binds and inhibits the activity of FK...
Homeodomain of yeast repressor alpha 2 contains a nuclear localization signal.
Hall, M N, Craik, C, Hiraoka, Y
The yeast repressor alpha 2 is shown, by analysis of deletion-bearing alpha 2-beta-galactosidase hybrid proteins, to have two structurally distinct nuclear localization signals. The cellular location...
Benson, S A, Hall, M N, Rasmussen, B A
The lamB701-708 signal sequence mutation reduces expression of LamB, an outer membrane protein of Escherichia coli. To investigate the possibility that synthesis and export of LamB are coupled, as...
Taylor, R K, Hall, M N, Enquist, L, Silhavy, T J
We report here on the cloning of a gene located within the ompB locus of E. coli K-12. This gene, designated ompR, resides on a 10.9-kilobase EcoRI fragment that we cloned, using a lambda vector and...
Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b.
Eleven independent insertion mutations were isolated that prevented expression of major outer membrane protein 1b. Seven of the mutations were Mucts insertions located at ombP. These ompB::Mucts...
TOR controls translation initiation and early G1 progression in yeast.
Barbet, N C, Schneider, U, Helliwell, S B, Stansfield, I, Tuite, M F, Hall, M N
Saccharomyces cerevisiae cells treated with the immunosuppressant rapamycin or depleted for the targets of rapamycin TOR1 and TOR2 arrest growth in the early G1 phase of the cell cycle. Loss of TOR...
Helliwell, S B, Wagner, P, Kunz, J, Deuter-Reinhard, M, Henriquez, R, Hall, M N
The Saccharomyces cerevisiae genes TOR1 and TOR2 were originally identified by mutations that confer resistance to the immunosuppressant rapamycin. TOR2 was previously shown to encode an essential...
Schmidt, A, Hall, M N, Koller, A
The macrocyclic lactone FK506 exerts immunosuppressive effects on T lymphocytes by interfering with signal transduction leading to T-cell activation and also inhibits the growth of eukaryotic...
The immunosuppressant FK506 inhibits amino acid import in Saccharomyces cerevisiae.
Heitman, J, Koller, A, Kunz, J, Henriquez, R, Schmidt, A, Movva, N R, ...
The immunosuppressants cyclosporin A, FK506, and rapamycin inhibit growth of unicellular eukaryotic microorganisms and also block activation of T lymphocytes from multicellular eukaryotes. In vitro,...
PIK1, an essential phosphatidylinositol 4-kinase associated with the yeast nucleus.
Garcia-Bustos, J F, Marini, F, Stevenson, I, Frei, C, Hall, M N
Transmission of mitogenic and developmental signals to intracellular targets is often mediated by inositol derivatives. Here we present the cloning and characterization of a gene from Saccharomyces...
Rapamycin blocks the phosphorylation of 4E-BP1 and inhibits cap-dependent initiation of translation.
Beretta, L, Gingras, A C, Svitkin, Y V, Hall, M N, Sonenberg, N
The immunosuppressant drug rapamycin blocks progression of the cell cycle at the G1 phase in mammalian cells and yeast. Here we show that rapamycin inhibits cap-dependent, but not cap-independent,...
Evidence for a coupling of synthesis and export of an outer membrane protein in Escherichia coli.
Hall, M N, Gabay, J, Schwartz, M
We describe a lesion, lamB701-708, affecting the hydrophilic portion of the lambda receptor signal sequence. The C to A transversion of the sixth codon of the signal sequence changes a positively...
Cell wall integrity modulates RHO1 activity via the exchange factor ROM2.
Bickle, M, Delley, P A, Schmidt, A, Hall, M N
The essential phosphatidylinositol kinase homologue TOR2 of Saccharomyces cerevisiae controls the actin cytoskeleton by activating a GTPase switch consisting of RHO1 (GTPase), ROM2 (GEF) and SAC7...
Schmidt, A, Beck, T, Koller, A, Kunz, J, Hall, M N
The Saccharomyces cerevisiae targets of rapamycin, TOR1 and TOR2, signal activation of cell growth in response to nutrient availability. Loss of TOR or rapamycin treatment causes yeast cells to...
Koller, A., Heitman, J., Hall, M. N.
In meiosis I, homologous chromosomes pair, recombine and segregate to opposite poles. These events and subsequent meiosis II ensure that each of the four meiotic products has one complete set of...
Danaie, P, Altmann, M, Hall, M N, Trachsel, H, Helliwell, S B
The essential cap-binding protein (eIF4E) of Saccharomyces cerevisiae is encoded by the CDC33 (wild-type) gene, originally isolated as a mutant, cdc33-1, which arrests growth in the G1 phase of the...
TOR2 is part of two related signaling pathways coordinating cell growth in Saccharomyces cerevisiae.
Helliwell, S B, Howald, I, Barbet, N, Hall, M N
The Saccharomyces cerevisiae genes TOR1 and TOR2 encode phosphatidylinositol kinase homologs. TOR2 has two essential functions. One function overlaps with TOR1 and mediates protein synthesis and cell...
Shaffer, H J, Hall, M N, Vander Bilt, J
OBJECTIVES: This study developed prevalence estimates of gambling-related disorders in the United States and Canada, identified differences in prevalence among population segments, and identified...