A STABLE AND EFFICIENT METHOD FOR TREATING SURFACE TENSION IN INCOMPRESSIBLE TWO-PHASE FLOW ∗ (2009)
This paper is dedicated to Diane Ohlhaber. Abstract. A new approach based on volume preserving motion by mean curvature for treating surface tension in two-phase flows is introduced. Many numerical...
An Improved Sharp Interface Method for Viscoelastic and Viscous Two-Phase Flows (2009)
J Sci Comput, P. A. Stewart, N. Lay, M. Sussman, M. Ohta
Abstract We introduce a robust method for computing viscous and viscoelastic two-phase bubble and drop motions. Our method utilizes a coupled level-set and volume-of-fluid technique for updating and...
Augsburger, L., Reymond, P., Fonck, E., Kulcsar, Z., Farhat, M., Ohta, M., ...
With intracranial aneurysms disease bringing a weakened arterial wall segment to initiate, grow and potentially rupture an aneurysm, current understanding of vessel wall biology perceives the disease...
M. Sussman, M. Ohta, Mark Sussman
stable and efficient method for treating surface
Exposure of tri-n-butyl tin chloride (TBTCl) as a stressor to Euglena gracilis Z causes rapid alteration of cell morphology followed by deflagellation. The present study was undertaken to reveal the...
Exposure of tri-n-butyl tin chloride (TBTCl) as a stressor to Euglena gracilis Z causes rapid alteration of cell morphology followed by deflagellation. The present study was undertaken to reveal the...
6th Tours Symposium on Nuclear Physics (2006)
Arnould, M, Lewitowicz, M, Emling, H, Akimune, H, Ohta, M, Utsunomiya, H, ...
Aritomo, Y., Ohta, M., Hanappe, F.
The fusion-fission process accompanied by neutron emission is studied in the superheavy-mass region on the basis of the fluctuation-dissipation model combined with a statistical model. The...
Ohta, M., Materna, T., Hanappe, F., Dorvaux, O., Stuttgé, L.
We analyzed the experimental data of the pre-scission neutron multiplicity in connection with fission fragments in the reaction $^{58}$Ni+$^{208}$Pb at the incident energy corresponding to the...
Origin of the drastic decrease of fusion probability in superheavy mass region (2005)
The fusion-fission process in the superheavy mass region is studied systematically by solving the time evolution of nuclear shape in three-dimensional deformation space using the Langevin equation....
5th Tours Symposium on Nuclear Physics (2005)
Akimune, H, Arnould, M, Lewitowicz, M, Münzenberg, G, Ohta, M, Utsunomiya, H, ...
4th Tours Symposium on Nuclear Physics (2005)
Akimune, H, Arnould, M, Lewitowicz, M, Oganessian, Yu T, Ohta, M, Utsunomiya, H, ...
Shizuma, T., Utsunomiya, H., Mohr, P., Hayakawa, T., Goko, S., Makinaga, A., ...
Cross sections of the $^{186}$W, $^{187}$Re, $^{188}$Os($\gamma,n$) reactions were measured using quasi-monochromatic photon beams from laser Compton scattering (LCS) with average energies from 7.3...
Trajectory analysis for fusion path in superheavy-mass region (2005)
We propose an effective method for the precise investigation of the fusion-fission mechanism in the superheavy-mass region, using the fluctuation-dissipation model. The trajectory calculation with...
Analysis of fusion-fission dynamics by pre-scission neutron emission in $^{58}$Ni+$^{208}$Pb (2005)
Aritomo, Y., Ohta, M., Materna, T., Hanappe, F., Dorvaux, O., Stuttge, L.
We analyzed the experimental data of the pre-scission neutron multiplicity in connection with fission fragments in the reaction $^{58}$Ni+$^{208}$Pb at the incident energy corresponding to the...
Two-stage laparoscopic resection of colon cancer and metastatic liver tumour (2005)
Iwashita, Y, Sasaki, A, Matsumoto, T, Shibata, K, Inomata, M, Ohta, M, ...
Ohta, M., Materna, T., Hanappe, F., Dorvaux, O., Stuttgé, L.
We analyzed the experimental data of the pre-scission neutron multiplicity in connection with fission fragments in the reaction $^{58}$Ni+$^{208}$Pb at the incident energy corresponding to the...
Ohta, M., Materna, T., Hanappe, F., Dorvaux, O., Stuttgé, L.
We analyzed the experimental data of the pre-scission neutron multiplicity in connection with fission fragments in the reaction $^{58}$Ni+$^{208}$Pb at the incident energy corresponding to the...
Nakagawa, M., Tanaka, F., Tsubota, N., Ohta, M., Takao, M., Wada, H., ...
Purpose: To examine the efficacy of UFT, an oral 5-fluorouracil derivative agent, as post-operative adjuvant therapy for pathologic (p-) stage I non-small-cell lung cancer (NSCLC), because a previous...
Dynamics of fusion-fission process in superheavy mass region (2004)
Aritomo, Y., Ohta, M., Materna, T., Hanappe, F., Stuttgé, L.
Tracking dissipation in capture reactions (2004)
Materna, T., Bouchat, V., Kinnard, V., Hanappe, F., Dorvaux, O., Schmitt, C., ...
Tracking dissipation in capture reactions (2004)
Materna, T., Bouchat, V., Kinnard, V., Hanappe, F., Dorvaux, O., Schmitt, C., ...
Dynamics of fusion-fission process with neutron evaporation in superheavy mass region (2004)
Aritomo, Y., Ohta, M., Materna, T., Hanappe, F., Stuttge, L.
Tracking dissipation in capture reactions (2004)
Materna, T., Bouchat, V., Kinnard, V., Hanappe, F., Dorvaux, O., Schmitt, C., ...
Dynamical calculation for fusion-fission process in superheavy mass region (2004)
Aritomo, Y., Ohta, M., Materna, T., Hanappe, F., Stuttge, L.
Dynamics of fusion-fission process in superheavy mass region (2004)
Aritomo, Y., Ohta, M., Materna, T., Hanappe, F., Stuttgé, L.
Tracking dissipation in capture reactions (2004)
Materna, T., Bouchat, V., Kinnard, V., Hanappe, F., Dorvaux, O., Schmitt, C., ...
Tracking dissipation in capture reactions (2004)
Materna, T., Bouchat, V., Kinnard, V., Hanappe, F., Dorvaux, O., Schmitt, C., ...
Dynamics of fusion-fission process with neutron evaporation in superheavy mass region (2004)
Aritomo, Y., Ohta, M., Materna, T., Hanappe, F., Stuttge, L.
Tracking dissipation in capture reactions (2004)
Materna, T., Bouchat, V., Kinnard, V., Hanappe, F., Dorvaux, O., Schmitt, C., ...
Dynamical calculation for fusion-fission process in superheavy mass region (2004)
Aritomo, Y., Ohta, M., Materna, T., Hanappe, F., Stuttge, L.
Ohta, M, Hyon, SH, Kang, YB, Murakami, S, Kohjiya, S, Oka, M, ...
Susceptibility of Calcium-Deficient Hydroxapatite Collagen Composite to Irradiation (2001)
Ohta, M., Yasuda, M., Okamura, H.
The ESR spectrum of calcium-deficient hydroxyapatite (DAp) has the peaks assigned to the hyperfine structure of dangling H+ (hyperfine coupling constant = 50.8 mT, I = 1/2) due to the HPO2-4 ion...
Surface reaction of magnesium in Hank's solutions (2000)
Kuwahara, H, Al-Abdullat, Y, Ohta, M, Tsutsumi, S, Ikeuchi, K, Mazaki, N, ...
Theory of Nuclear Reactions Leading to Superheavy Elements (1999)
Abe, Y., Okazaki, K., Aritomo, Y., Wada, T., Ohta, M.
Dynamical reaction theory is presented for synthesis of superheavy elements. Characteristic features of formation and surviving are discussed, which combinedly determine final residue cross sections...
Theoretical predictions of residues cross sections of superheavy elements (1999)
Abe, Y., Okazaki, K., Aritomo, Y., Tokuda, T., Wada, T., Ohta, M.
Dynamical reaction theories are reviewed for synthesis of superheavy elements. Characteristic features of formation and surviving are discussed with reference to possible incident channels....
A case of multiple endocrine neoplasia type 2B (1997)
Ohta, M, Tokuda, Y, Suzuki, Y, Kuge, S, Okumura, A, Kubota, M, ...
A case of angiosarcoma of the breast (1997)
Ohta, M, Tokuda, Y, Kuge, S, Okumura, A, Tanaka, M, Kubota, M, ...
Use of metronidazole gel to control malodor in advanced and recurrent breast cancer (1996)
Kuge, S, Tokuda, Y, Ohta, M, Okumura, A, Kubota, M, Ninomiya, S, ...
Hakim, A., Fahli, A., Ohta, M., Guillaume, G., Coffin, J.P., Fintz, P., ...
Hakim, A., Fahli, A., Ohta, M., Guillaume, G., Coffin, J.P., Fintz, P., ...
Hakim, A., Fahli, A., Ohta, M., Guillaume, G., Coffin, J.P., Fintz, P., ...
Hakim, A., Fahli, A., Ohta, M., Guillaume, G., Coffin, J.P., Fintz, P., ...
Prompt and delayed light particle emission in heavy ion reactions (1988)
Coffin, J.P., Ohta, M., Malki, A., Fintz, P., Gonin, M., Guillaume, G., ...
Prompt and delayed light particle emission in heavy ion reactions (1988)
Coffin, J.P., Ohta, M., Malki, A., Fintz, P., Gonin, M., Guillaume, G., ...
FHIT gene alterations in head and neck squamous cell carcinomas.
Virgilio, L, Shuster, M, Gollin, S M, Veronese, M L, Ohta, M, Huebner, K, ...
To determine whether the FHIT gene at 3p14.2 is altered in head and neck squamous cell carcinomas (HNSCC), we examined 26 HNSCC cell lines for deletions within the FHIT locus by Southern analysis,...
Morimoto, S., Harada, K., Takahashi-Yanaga, F., Minakami, R., Ohta, M., ...
A deletion mutation ΔK210 in cardiac troponin T (cTnT) was recently found to cause familial dilated cardiomyopathy (DCM). To explore the effect of this mutation on cardiac muscle contraction under...
Ito, H, Arakawa, Y, Ohsuka, S, Wacharotayankun, R, Kato, N, Ohta, M
The distribution of strains producing metallo-beta-lactamase among 105 strains of Serratia marcescens was investigated. All of these strains were isolated in seven general hospitals located in Aichi...
A novel integron-like element carrying the metallo-beta-lactamase gene blaIMP.
Arakawa, Y, Murakami, M, Suzuki, K, Ito, H, Wacharotayankun, R, Ohsuka, S, ...
A plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant Serratia marcescens strain, AK9373. The metallo-beta-lactamase gene was identical to the blaIMP, and it was...
Ohsuka, S, Arakawa, Y, Horii, T, Ito, H, Ohta, M
The effects of acidic conditions on activities of seven beta-lactamases--TEM-1 (class A), KOXY (class A), IMP-1 (class B), AmpC (class C), MOX-1 (class C), OXA-5 (class D), and PSE-2 (class D)--and...
Senda, K, Arakawa, Y, Nakashima, K, Ito, H, Ichiyama, S, Shimokata, K, ...
A total of 3,700 Pseudomonas aeruginosa isolates were collected from 17 general hospitals in Japan from 1992 to 1994. Of these isolates, 132 carbapenem-resistant strains were subjected to DNA...
Kimura, K, Arakawa, Y, Ohsuka, S, Ito, H, Suzuki, K, Kurokawa, H, ...
Nine Klebsiella oxytoca strains which demonstrated resistance to the combination of sulbactam and cefoperazone were isolated from geographically separate hospitals in Japan in 1995. Among them, K....
Yagi, T, Kurokawa, H, Senda, K, Ichiyama, S, Ito, H, Ohsuka, S, ...
Escherichia coli HKY56, which demonstrated resistance to various beta-lactams except carbapenems, was isolated from the throat swab of an inpatient in 1994. Conjugal transfer of cephem resistance...
Arakawa, Y, Ohta, M, Kido, N, Mori, M, Ito, H, Komatsu, T, ...
The chromosomally encoded beta-lactamase gene of Klebsiella oxytoca E23004, a strain resistant to cefoperazone and aztreonam, was cloned and expressed in Escherichia coli HB101. The molecular mass...
Arakawa, Y, Wacharotayankun, R, Nagatsuka, T, Ito, H, Kato, N, Ohta, M
The genomic organization of the chromosomal cps region that is responsible for capsular polysaccharide synthesis in Klebsiella pneumoniae Chedid (O1:K2) was investigated. Deletion analyses and...
Possible mechanisms underlying the slow lactose fermentation phenotype in Shigella spp.
Ito, H, Kido, N, Arakawa, Y, Ohta, M, Sugiyama, T, Kato, N
A Southern hybridization analysis revealed that the region homologous to Escherichia coli lacZ was present on the chromosomal DNAs of beta-galactosidase-positive Shigella strains, such as Shigella...
Horii, T, Arakawa, Y, Ohta, M, Ichiyama, S, Wacharotayankun, R, Kato, N
Klebsiella pneumoniae NU2936 was isolated from a patient and was found to produce a plasmid-encoded beta-lactamase (MOX-1) which conferred resistance to broad spectrum beta-lactams, including...
Ohta, M, Ito, H, Masuda, K, Tanaka, S, Arakawa, Y, Wacharotayankun, R, ...
Tachyplesins I and II and polyphemusins I and II, cationic peptides isolated from the hemocytes of horseshoe crabs, show bactericidal activities with similar efficiencies for both gram-negative and...
Involvement of rcsB in Klebsiella K2 capsule synthesis in Escherichia coli K-12.
Wacharotayankun, R, Arakawa, Y, Ohta, M, Hasegawa, T, Mori, M, Horii, T, ...
Escherichia coli K-12 harboring a part of the structural genes for the Klebsiella K2 capsular polysaccharide (cpsK*) expresses a large amount of K2 capsular polysaccharide as a thick capsule in the...
Sugiyama, T, Kido, N, Komatsu, T, Ohta, M, Kato, N
To investigate the effect of chromosomal mutation on the synthesis of rfe-dependent Escherichia coli O9 lipopolysaccharide (LPS), the cloned E. coli O9 rfb gene was introduced into Salmonella...
A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with...
Crystallization of R-form lipopolysaccharides from Salmonella minnesota and Escherichia coli.
Kato, N, Ohta, M, Kido, N, Ito, H, Naito, S, Hasegawa, T, ...
Salmonella minnesota Re and Ra lipopolysaccharides (LPSs) and Escherichia coli K-12 LPS formed three-dimensional crystals, either hexagonal plates (preferential growth along the a axis) or solid...
Kido, N, Ohta, M, Iida, K, Hasegawa, T, Ito, H, Arakawa, Y, ...
The rfb gene, involved in the synthesis of the O-specific polysaccharide (a mannose homopolymer) of Escherichia coli O9 lipopolysaccharide (LPS), was cloned in E. coli K-12 strains. The O9-specific...
Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Mizuno, T, ...
The introduction of a newly cloned Enterobacter cloacae chromosomal gene romA, into Escherichia coli and E. cloacae resulted in enhancement of resistance to quinolones, beta-lactams, chloramphenicol,...
Formation of a hexagonal lattice structure by an R-form lipopolysaccharide of Klebsiella sp.
Kato, N, Ohta, M, Kido, N, Ito, H, Naito, S, Kuno, T
We extracted an R-form lipopolysaccharide (LPS) by the phenol-water method from Klebsiella sp. strain LEN-111 (O3-:KI-) and followed the changes in ultrastructure of the LPS during the extraction...
Senda, K, Arakawa, Y, Ichiyama, S, Nakashima, K, Ito, H, Ohsuka, S, ...
We applied PCR to the rapid detection of the metallo-beta-lactamase gene, blaIMP, in clinically isolated gram-negative rods. A total of 54 high-level ceftazidime-resistant strains (MICs, > 128...
Kawabata, A, Ichiyama, S, Iinuma, Y, Hasegawa, Y, Ohta, M, Shimokata, K
A rapid and simple method for detecting exfoliative toxin serotypes A and B from clinical isolates has been developed as a test kit (EXT-RPLA; Denka Seiken Co. Ltd., Niigata, Japan). This method is...
Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Kato, N
Introduction of the romA gene cloned from Enterobacter cloacae into Escherichia coli K-12 resulted in almost complete inhibition of OmpF expression and a concomitant increase in resistance to...
Ohta, M, Nyunoya, H, Tanaka, H, Okamoto, T, Akagi, T, Shimotohno, K
The X gene products of the human T-cell leukemia viruses type I and II are thought to be involved not only in viral replication but also in mediating the expression of certain cellular genes. These X...
Arakawa, Y, Ohta, M, Wacharotayankun, R, Mori, M, Kido, N, Ito, H, ...
The genes determining the biosynthesis of type 2 (K2) capsular polysaccharide [3----beta Glc1,4----beta Man(1,3----beta GlcUA) 1,4----alpha Glc1----] of Klebsiella pneumoniae Chedid (O1:K2), which is...
Ichinose, Y, Hara, N, Ohta, M, Aso, H, Chikama, H, Kawasaki, M, ...
Superoxide anion (O2-) generation by human blood neutrophils induced by phorbol myristate acetate, formyl-methionyl-leucyl-phenylalanine, and monoclonal antibody YI51 was measured 24 h after...
Ichinose, Y, Yagawa, K, Kaku, M, Hara, N, Ohta, M
The effect of phorbol myristate acetate (PMA) on the antibody-dependent cellular cytotoxicity (ADCC) of human polymorphonuclear leukocytes (PMNs) against human erythroleukemic K562 cells was studied...
Mizuta, K, Ohta, M, Mori, M, Hasegawa, T, Nakashima, I, Kato, N
Eighty-two Klebsiella O1 strains belonging to various K types were tested for virulence for mice by intraperitoneal inoculation. They comprised 49 strains newly isolated from various clinical...
Ichiyama, S, Ohta, M, Shimokata, K, Kato, N, Takeuchi, J
In this study, we have compared genomic DNA fingerprintings among isolates of methicillin-resistant Staphylococcus aureus (MRSA) by using pulsed-field gel electrophoresis (PFGE). Chromosomal...
Wacharotayankun, R, Arakawa, Y, Ohta, M, Tanaka, K, Akashi, T, Mori, M, ...
We determined the complete nucleotide sequence of a 2.1-kb HindIII-EcoRI fragment that was cloned from a resident large plasmid of Klebsiella pneumoniae Chedid, a highly virulent and mucoviscous...
Osano, E, Arakawa, Y, Wacharotayankun, R, Ohta, M, Horii, T, Ito, H, ...
A clinical isolate of Serratia marcescens (TN9106) produced a metallo beta-lactamase (IMP-1) which conferred resistance to imipenem and broad-spectrum beta-lactams. The blaIMP gene providing imipenem...
Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi.
Ohashi, N, Tamura, A, Ohta, M, Hayashi, K
A type-specific antigen (54- to 56-kilodalton polypeptide) in the envelope of Rickettsia tsutsugamushi was purified from each of three prototype strains (Gilliam, Karp, and Kato) by a combination of...
Ichinose, Y, Hara, N, Ohta, M, Motohiro, A, Kuda, T, Aso, H, ...
We used a monoclonal antibody, YI 51, raised against human polymorphonuclear leukocytes (PMN) to induce superoxide anion (O2-) generation in cells. Although YI 51 alone played only a small part in...
Microinjected pBR322 stimulates cellular DNA synthesis in Swiss 3T3 cells.
Hyland, J K, Hirschhorn, R R, Avignolo, C, Mercer, W E, Ohta, M, Galanti, N, ...
When pBR322 is manually microinjected into the nuclei of quiescent Swiss 3T3 cells it stimulates the incorporation of [3H]thymidine into DNA. The evidence clearly shows that this increased...
Kitagawa, S, Ohta, M, Nojiri, H, Kakinuma, K, Saito, M, Takaku, F, ...
The alterations of stimulus-induced membrane potential changes, superoxide (O2-)-producing capacity and phagocytic activity during differentiation of human granulocytes were investigated in the human...
Kimura, K, Shiota, M, Mochizuki, K, Ohta, M, Sugano, T
Zymosan (non-boiled) induced glycogenolysis biphasically, with no lag time, in the perfused rat liver. After the zymosan was boiled, it could be separated into two fractions, both of which stimulated...
C-terminal topology of gastric H+,K(+)-ATPase.
Asano, S, Arakawa, S, Hirasawa, M, Sakai, H, Ohta, M, Ohta, K, ...
An antibody was prepared against a peptide corresponding to residues 1024-1034 (the putative C-terminus) of the alpha-subunit of hog gastric H+,K(+)-ATPase. The antibody bound to a 95 kDa band of...
Congenital absence of the portal vein.
Nakasaki, H, Tanaka, Y, Ohta, M, Kanemoto, T, Mitomi, T, Iwata, Y, ...
A 14-year-old girl presented at the hospital after discovering an abdominal tumor. CT scan and ultrasonography indicated a hepatic tumor and also revealed the absence of the portal vein. The patient...
Fujimoto, S, Shrestha, R D, Kokubun, M, Kobayashi, K, Kiuchi, S, Konno, C, ...
To evaluate the clinical efficacy of intraperitoneal hyperthermic perfusion (IPHP) for far-advanced gastric cancer, particularly with peritoneal seeding, we investigated the survival times of 59...
Fujimoto, S, Shrestha, R D, Kokubun, M, Ohta, M, Takahashi, M, Kobayashi, K, ...
Fifteen patients with far-advanced gastric cancer were given surgical treatment followed by intraperitoneal hyperthermic perfusion (IPHP) with mitomycin C (MMC) and misonidazole (MIS), a...
FHIT gene alterations in head and neck squamous cell carcinomas.
Virgilio, L, Shuster, M, Gollin, S M, Veronese, M L, Ohta, M, Huebner, K, ...
To determine whether the FHIT gene at 3p14.2 is altered in head and neck squamous cell carcinomas (HNSCC), we examined 26 HNSCC cell lines for deletions within the FHIT locus by Southern analysis,...
Morimoto, S., Harada, K., Takahashi-Yanaga, F., Minakami, R., Ohta, M., ...
A deletion mutation ΔK210 in cardiac troponin T (cTnT) was recently found to cause familial dilated cardiomyopathy (DCM). To explore the effect of this mutation on cardiac muscle contraction under...
Ito, H, Arakawa, Y, Ohsuka, S, Wacharotayankun, R, Kato, N, Ohta, M
The distribution of strains producing metallo-beta-lactamase among 105 strains of Serratia marcescens was investigated. All of these strains were isolated in seven general hospitals located in Aichi...
A novel integron-like element carrying the metallo-beta-lactamase gene blaIMP.
Arakawa, Y, Murakami, M, Suzuki, K, Ito, H, Wacharotayankun, R, Ohsuka, S, ...
A plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant Serratia marcescens strain, AK9373. The metallo-beta-lactamase gene was identical to the blaIMP, and it was...
Ohsuka, S, Arakawa, Y, Horii, T, Ito, H, Ohta, M
The effects of acidic conditions on activities of seven beta-lactamases--TEM-1 (class A), KOXY (class A), IMP-1 (class B), AmpC (class C), MOX-1 (class C), OXA-5 (class D), and PSE-2 (class D)--and...
Senda, K, Arakawa, Y, Nakashima, K, Ito, H, Ichiyama, S, Shimokata, K, ...
A total of 3,700 Pseudomonas aeruginosa isolates were collected from 17 general hospitals in Japan from 1992 to 1994. Of these isolates, 132 carbapenem-resistant strains were subjected to DNA...
Kimura, K, Arakawa, Y, Ohsuka, S, Ito, H, Suzuki, K, Kurokawa, H, ...
Nine Klebsiella oxytoca strains which demonstrated resistance to the combination of sulbactam and cefoperazone were isolated from geographically separate hospitals in Japan in 1995. Among them, K....
Yagi, T, Kurokawa, H, Senda, K, Ichiyama, S, Ito, H, Ohsuka, S, ...
Escherichia coli HKY56, which demonstrated resistance to various beta-lactams except carbapenems, was isolated from the throat swab of an inpatient in 1994. Conjugal transfer of cephem resistance...
Arakawa, Y, Ohta, M, Kido, N, Mori, M, Ito, H, Komatsu, T, ...
The chromosomally encoded beta-lactamase gene of Klebsiella oxytoca E23004, a strain resistant to cefoperazone and aztreonam, was cloned and expressed in Escherichia coli HB101. The molecular mass...
Arakawa, Y, Wacharotayankun, R, Nagatsuka, T, Ito, H, Kato, N, Ohta, M
The genomic organization of the chromosomal cps region that is responsible for capsular polysaccharide synthesis in Klebsiella pneumoniae Chedid (O1:K2) was investigated. Deletion analyses and...
Possible mechanisms underlying the slow lactose fermentation phenotype in Shigella spp.
Ito, H, Kido, N, Arakawa, Y, Ohta, M, Sugiyama, T, Kato, N
A Southern hybridization analysis revealed that the region homologous to Escherichia coli lacZ was present on the chromosomal DNAs of beta-galactosidase-positive Shigella strains, such as Shigella...
Horii, T, Arakawa, Y, Ohta, M, Ichiyama, S, Wacharotayankun, R, Kato, N
Klebsiella pneumoniae NU2936 was isolated from a patient and was found to produce a plasmid-encoded beta-lactamase (MOX-1) which conferred resistance to broad spectrum beta-lactams, including...
Ohta, M, Ito, H, Masuda, K, Tanaka, S, Arakawa, Y, Wacharotayankun, R, ...
Tachyplesins I and II and polyphemusins I and II, cationic peptides isolated from the hemocytes of horseshoe crabs, show bactericidal activities with similar efficiencies for both gram-negative and...
Involvement of rcsB in Klebsiella K2 capsule synthesis in Escherichia coli K-12.
Wacharotayankun, R, Arakawa, Y, Ohta, M, Hasegawa, T, Mori, M, Horii, T, ...
Escherichia coli K-12 harboring a part of the structural genes for the Klebsiella K2 capsular polysaccharide (cpsK*) expresses a large amount of K2 capsular polysaccharide as a thick capsule in the...
Sugiyama, T, Kido, N, Komatsu, T, Ohta, M, Kato, N
To investigate the effect of chromosomal mutation on the synthesis of rfe-dependent Escherichia coli O9 lipopolysaccharide (LPS), the cloned E. coli O9 rfb gene was introduced into Salmonella...
A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with...
Crystallization of R-form lipopolysaccharides from Salmonella minnesota and Escherichia coli.
Kato, N, Ohta, M, Kido, N, Ito, H, Naito, S, Hasegawa, T, ...
Salmonella minnesota Re and Ra lipopolysaccharides (LPSs) and Escherichia coli K-12 LPS formed three-dimensional crystals, either hexagonal plates (preferential growth along the a axis) or solid...
Kido, N, Ohta, M, Iida, K, Hasegawa, T, Ito, H, Arakawa, Y, ...
The rfb gene, involved in the synthesis of the O-specific polysaccharide (a mannose homopolymer) of Escherichia coli O9 lipopolysaccharide (LPS), was cloned in E. coli K-12 strains. The O9-specific...
Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Mizuno, T, ...
The introduction of a newly cloned Enterobacter cloacae chromosomal gene romA, into Escherichia coli and E. cloacae resulted in enhancement of resistance to quinolones, beta-lactams, chloramphenicol,...
Formation of a hexagonal lattice structure by an R-form lipopolysaccharide of Klebsiella sp.
Kato, N, Ohta, M, Kido, N, Ito, H, Naito, S, Kuno, T
We extracted an R-form lipopolysaccharide (LPS) by the phenol-water method from Klebsiella sp. strain LEN-111 (O3-:KI-) and followed the changes in ultrastructure of the LPS during the extraction...
Senda, K, Arakawa, Y, Ichiyama, S, Nakashima, K, Ito, H, Ohsuka, S, ...
We applied PCR to the rapid detection of the metallo-beta-lactamase gene, blaIMP, in clinically isolated gram-negative rods. A total of 54 high-level ceftazidime-resistant strains (MICs, > 128...
Kawabata, A, Ichiyama, S, Iinuma, Y, Hasegawa, Y, Ohta, M, Shimokata, K
A rapid and simple method for detecting exfoliative toxin serotypes A and B from clinical isolates has been developed as a test kit (EXT-RPLA; Denka Seiken Co. Ltd., Niigata, Japan). This method is...
Komatsu, T, Ohta, M, Kido, N, Arakawa, Y, Ito, H, Kato, N
Introduction of the romA gene cloned from Enterobacter cloacae into Escherichia coli K-12 resulted in almost complete inhibition of OmpF expression and a concomitant increase in resistance to...
Ohta, M, Nyunoya, H, Tanaka, H, Okamoto, T, Akagi, T, Shimotohno, K
The X gene products of the human T-cell leukemia viruses type I and II are thought to be involved not only in viral replication but also in mediating the expression of certain cellular genes. These X...
Arakawa, Y, Ohta, M, Wacharotayankun, R, Mori, M, Kido, N, Ito, H, ...
The genes determining the biosynthesis of type 2 (K2) capsular polysaccharide [3----beta Glc1,4----beta Man(1,3----beta GlcUA) 1,4----alpha Glc1----] of Klebsiella pneumoniae Chedid (O1:K2), which is...
Ichinose, Y, Hara, N, Ohta, M, Aso, H, Chikama, H, Kawasaki, M, ...
Superoxide anion (O2-) generation by human blood neutrophils induced by phorbol myristate acetate, formyl-methionyl-leucyl-phenylalanine, and monoclonal antibody YI51 was measured 24 h after...
Ichinose, Y, Yagawa, K, Kaku, M, Hara, N, Ohta, M
The effect of phorbol myristate acetate (PMA) on the antibody-dependent cellular cytotoxicity (ADCC) of human polymorphonuclear leukocytes (PMNs) against human erythroleukemic K562 cells was studied...
Mizuta, K, Ohta, M, Mori, M, Hasegawa, T, Nakashima, I, Kato, N
Eighty-two Klebsiella O1 strains belonging to various K types were tested for virulence for mice by intraperitoneal inoculation. They comprised 49 strains newly isolated from various clinical...
Ichiyama, S, Ohta, M, Shimokata, K, Kato, N, Takeuchi, J
In this study, we have compared genomic DNA fingerprintings among isolates of methicillin-resistant Staphylococcus aureus (MRSA) by using pulsed-field gel electrophoresis (PFGE). Chromosomal...
Wacharotayankun, R, Arakawa, Y, Ohta, M, Tanaka, K, Akashi, T, Mori, M, ...
We determined the complete nucleotide sequence of a 2.1-kb HindIII-EcoRI fragment that was cloned from a resident large plasmid of Klebsiella pneumoniae Chedid, a highly virulent and mucoviscous...
Osano, E, Arakawa, Y, Wacharotayankun, R, Ohta, M, Horii, T, Ito, H, ...
A clinical isolate of Serratia marcescens (TN9106) produced a metallo beta-lactamase (IMP-1) which conferred resistance to imipenem and broad-spectrum beta-lactams. The blaIMP gene providing imipenem...
Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi.
Ohashi, N, Tamura, A, Ohta, M, Hayashi, K
A type-specific antigen (54- to 56-kilodalton polypeptide) in the envelope of Rickettsia tsutsugamushi was purified from each of three prototype strains (Gilliam, Karp, and Kato) by a combination of...
Ichinose, Y, Hara, N, Ohta, M, Motohiro, A, Kuda, T, Aso, H, ...
We used a monoclonal antibody, YI 51, raised against human polymorphonuclear leukocytes (PMN) to induce superoxide anion (O2-) generation in cells. Although YI 51 alone played only a small part in...
Microinjected pBR322 stimulates cellular DNA synthesis in Swiss 3T3 cells.
Hyland, J K, Hirschhorn, R R, Avignolo, C, Mercer, W E, Ohta, M, Galanti, N, ...
When pBR322 is manually microinjected into the nuclei of quiescent Swiss 3T3 cells it stimulates the incorporation of [3H]thymidine into DNA. The evidence clearly shows that this increased...
Kitagawa, S, Ohta, M, Nojiri, H, Kakinuma, K, Saito, M, Takaku, F, ...
The alterations of stimulus-induced membrane potential changes, superoxide (O2-)-producing capacity and phagocytic activity during differentiation of human granulocytes were investigated in the human...
Kimura, K, Shiota, M, Mochizuki, K, Ohta, M, Sugano, T
Zymosan (non-boiled) induced glycogenolysis biphasically, with no lag time, in the perfused rat liver. After the zymosan was boiled, it could be separated into two fractions, both of which stimulated...
C-terminal topology of gastric H+,K(+)-ATPase.
Asano, S, Arakawa, S, Hirasawa, M, Sakai, H, Ohta, M, Ohta, K, ...
An antibody was prepared against a peptide corresponding to residues 1024-1034 (the putative C-terminus) of the alpha-subunit of hog gastric H+,K(+)-ATPase. The antibody bound to a 95 kDa band of...
Congenital absence of the portal vein.
Nakasaki, H, Tanaka, Y, Ohta, M, Kanemoto, T, Mitomi, T, Iwata, Y, ...
A 14-year-old girl presented at the hospital after discovering an abdominal tumor. CT scan and ultrasonography indicated a hepatic tumor and also revealed the absence of the portal vein. The patient...
Fujimoto, S, Shrestha, R D, Kokubun, M, Kobayashi, K, Kiuchi, S, Konno, C, ...
To evaluate the clinical efficacy of intraperitoneal hyperthermic perfusion (IPHP) for far-advanced gastric cancer, particularly with peritoneal seeding, we investigated the survival times of 59...
Fujimoto, S, Shrestha, R D, Kokubun, M, Ohta, M, Takahashi, M, Kobayashi, K, ...
Fifteen patients with far-advanced gastric cancer were given surgical treatment followed by intraperitoneal hyperthermic perfusion (IPHP) with mitomycin C (MMC) and misonidazole (MIS), a...
Nakashima, I, Nagase, F, Yokochi, T, Kojima, T, Ohta, M, Kato, N
Using the capsular polysaccharide of Klebsiella pneumoniae (CPS-K) as a polyclonal B-cell activator (PBA) and sheep red blood cells (SRBC) as a T cell-dependent antigen, we compared the ability of...
Contribution of the mannan O side-chains to the adjuvant action of lipopolysaccharides.
Ohta, M, Kido, N, Hasegawa, T, Ito, H, Fujii, Y, Arakawa, Y, ...
Klebsiella 03 lipopolysaccharide (LPS) and Escherichia coli 09 LPS were previously shown to have potent adjuvant activities in augmenting antibody response and delayed-type hypersensitivity to...
Kato, N, Kido, N, Ohta, M, Naito, S
Previously, we found that Klebsiella O3 lipopolysaccharide (KO3 LPS) exhibits very strong adjuvant activity in augmenting the delayed-type hypersensitivity and antibody responses to protein antigens,...
Adjuvant actions of linear mannan-possessing lipopolysaccharide (LPS) in LPS-resistant C3H/HeJ mice.
Ohta, M, Kido, N, Nakashima, I, Kato, N
Immunopotentiation has been demonstrated when Klebsiella O3 lipopolysaccharide (KO3 LPS), which possesses a linear mannan as the O-specific side chain, was injected subcutaneously into endotoxin...
Ohta, M., Marceau, N., French, S. W.
The changes in cytokeratin intermedial filaments (IFs) after cytochalasin B (CB) infusion of rat liver in vivo were studied by light and electron microscopy, immunofluorescent staining (IMF), and...
Kiuchi, K, Nejima, J, Takano, T, Ohta, M, Hashimoto, H, BAXTER, G
OBJECTIVE—To assess whether the concentrations of serum advanced glycation end products (AGE) in diabetic patients with obstructive coronary artery disease differ from those in type 2 diabetic...
We established six human T cell lines derived from rheumatoid arthritis synovial fluid (RASF). Phenotypically, T cell receptor (TCR) gamma delta T cells occupied the majority of these lines and most...
Blockade of the reticulospinal inhibitory pathway by anaesthetic agents
1. Adult cats were decerebrated at the intercollicular level. The effect of the anaesthetic agents, pentobarbitone, paraldehyde, tribromethanol, chloralose and procaine on the reticulospinal...
Blockade of the reticulospinal inhibitory pathway by nitrous oxide and tetrodotoxin
1. The effects of nitrous oxide and tetrodotoxin were investigated on the reticulospinal inhibitory pathway in the acutely decerebrated cat.
Meta-analysis of α synuclein/ NACP polymorphism in Parkinson's disease in Japan
Mizuta, I, Nishimura, M, Mizuta, E, Yamasaki, S, Ohta, M, Kuno, S
Fujimoto, S., Kobayashi, K., Takahashi, M., Konno, C., Kokubun, M., Ohta, M., ...
We examined the effects of misonidazole (MISO) and recombinant human tumour necrosis factor (rh-TNF) on tumour blood flow in mice given hyperthermic treatments. MISO (500 mg kg-1) or rh-TNF (6 x...
Fujimoto, S., Ohta, M., Shrestha, R. D., Kokubun, M., Miyoshi, T., Mori, T., ...
Hyperthermia for human gastric cancer xenotransplanted into the hindlegs of nude mice was performed to determine whether misonidazole (MISO) or metronidazole (MTR), derivatives of nitroimidazole,...
Clinical significance of the reduced expression of G protein gamma 7 (GNG7) in oesophageal cancer
Ohta, M, Mimori, K, Fukuyoshi, Y, Kita, Y, Motoyama, K, Yamashita, K, ...
We previously cloned human G protein gamma 7 (GNG7) and demonstrated that it was downregulated in gastrointestinal cancer. The significance of GNG7 expression in oesophageal cancer is unknown. TaqMan...
Tokuda, Y, Watanabe, T, Omuro, Y, Ando, M, Katsumata, N, Okumura, A, ...
We conducted a phase I pharmacokinetic dose escalation study of a recombinant humanized anti-p185HER2 monoclonal antibody (MKC-454) in 18 patients with metastatic breast cancer refractory to...