Subcellulosome preparation with high cellulase activity from Clostridium thermocellum.
Kobayashi, T, Romaniec, M P, Fauth, U, Demain, A L
We have prepared a much simpler cellulase preparation than that of cellulosomes from the extracellular broth of Clostridium thermocellum. This "subcellulosome" preparation from C. thermocellum was...
Purification and characterization of a new endoglucanase from Clostridium thermocellum.
Romaniec, M P, Fauth, U, Kobayashi, T, Huskisson, N S, Barker, P J, Demain, A L
An endoglucanase (1,4-beta-D-glucan glucanohydrolase, EC 3.2.1.4) from the thermophilic anaerobe Clostridium thermocellum was purified to apparent homogeneity without the use of denaturants. No...
Thermostable chaperonin from Clostridium thermocellum.
Cross, S J, Ciruela, A, Poomputsa, K, Romaniec, M P, Freedman, R B
Homologues of the chaperonins Cpn60 and Cpn10 have been purified from the Gram-positive cellulolytic thermophile Clostridium thermocellum. The Cpn60 protein was purified by ATP-affinity...
Purification and characterization of endoglucanase Ss from Clostridium thermocellum.
Fauth, U, Romaniec, M P, Kobayashi, T, Demain, A L
The extracellular cellulolytic enzymes of the thermophilic anaerobe Clostridium thermocellum occur as a protein complex or aggregate known as the cellulosome. By using a combination of ion-exchange,...
Subcellulosome preparation with high cellulase activity from Clostridium thermocellum.
Kobayashi, T, Romaniec, M P, Fauth, U, Demain, A L
We have prepared a much simpler cellulase preparation than that of cellulosomes from the extracellular broth of Clostridium thermocellum. This "subcellulosome" preparation from C. thermocellum was...
Purification and characterization of a new endoglucanase from Clostridium thermocellum.
Romaniec, M P, Fauth, U, Kobayashi, T, Huskisson, N S, Barker, P J, Demain, A L
An endoglucanase (1,4-beta-D-glucan glucanohydrolase, EC 3.2.1.4) from the thermophilic anaerobe Clostridium thermocellum was purified to apparent homogeneity without the use of denaturants. No...
Purification and characterization of endoglucanase Ss from Clostridium thermocellum.
Fauth, U, Romaniec, M P, Kobayashi, T, Demain, A L
The extracellular cellulolytic enzymes of the thermophilic anaerobe Clostridium thermocellum occur as a protein complex or aggregate known as the cellulosome. By using a combination of ion-exchange,...
Thermostable chaperonin from Clostridium thermocellum.
Cross, S J, Ciruela, A, Poomputsa, K, Romaniec, M P, Freedman, R B
Homologues of the chaperonins Cpn60 and Cpn10 have been purified from the Gram-positive cellulolytic thermophile Clostridium thermocellum. The Cpn60 protein was purified by ATP-affinity...