M. Sundaralingam

Publication List Details

Period

1998 - 1998

Number

63

Co-Authors

A NUCLEAR QUADRUPOLE RESONANCE AND X-RAY STUDY OF THE CRYSTAL STRUCTURE OF 2,5DICHLOROANILINE, (1998)

Sakurai,T., Sundaralingam,M., Jeffrey,G. A.

The crystal structure of 2,5-dichloroaniline, C6H3Cl2NH2, was determined by the combined application of nuclear quadrupole resonance and X-ray diffraction. The molecule is planar, except for the...

A novel end-to-end binding of two netropsins to the DNA decamers d(CCCCCIIIII)2, d(CCCBr5CCIIIII)2and d(CBr5CCCCIIIII)2.

Chen, X, Mitra, S N, Rao, S T, Sekar, K, Sundaralingam, M

Netropsin is bound to the DNA decamer d(CCCCCIIIII)2, the C-4 bromo derivative d(CCCBr5CCIIIII)2and the C-2 bromo derivative d(CBr5CCCCIIIII)2in a novel 2:1 mode. Complexes of the native decamer and...

Crystal structure of an RNA octamer duplex r(CCCIUGGG)2 incorporating tandem I.U wobbles.

Pan, B, Mitra, S N, Sun, L, Hart, D, Sundaralingam, M

The crystal structure of the RNA octamer duplex r(CCCIUGGG)2has been elucidated at 2.5 A resolution. The crystals belong to the space group P21and have unit cell constants a = 33.44 A, b = 43.41 A, c...

Crystal structure of an RNA duplex r(G GCGC CC)2 with non-adjacent G*U base pairs.

Shi, K, Wahl, M, Sundaralingam, M

The crystal structure of a self-complementary RNA duplex r(GGGCGCUCC)2with non-adjacent G*U and U*G wobble pairs separated by four Watson-Crick base pairs has been determined to 2.5 A resolution....

STEREOCHEMISTRY OF NUCLEIC ACIDS AND THEIR CONSTITUENTS, XI. THE CONFORMATION OF THE ANTIBIOTIC PUROMYCIN DIHYDROCHLORIDE PENTAHYDRATE*†

Sundaralingam, M., Arora, S. K.

The elucidation of puromycin structure represents the first case where both a nucleic acid and a protein fragment are found together in a crystal. The molecule displays an elongated conformation with...

Biological Membrane Structure, II. A Detailed Model for the Retinal Rod Outer Segment Membrane

Vanderkooi, G., Sundaralingam, M.

A model, based on the available chemical, electron microscopic, and x-ray diffraction data, has been developed for the structure of the retinal rod outer segment membranes. The model consists of a...

X-ray structure of cytidine-5'-O-dimethylphosphate. Novel stacking between the ribosyl O(2') hydroxyl oxygen atom and the base.

Brennan, R G, Kondo, N S, Sundaralingam, M

The anionic oxygen atoms of the phosphodiester backbone of RNA and DNA are particularly susceptible to esterification by many mutagenic and carcinogenic alkylating agents. To better understand the...

Similar binding of the carcinostatic drugs cis-[Pt(NH3)2Cl2] and [Ru(NH3)5Cl] Cl2 to tRNAphe and a comparison with the binding of the inactive trans-[Pt(NH3)2Cl2] complex - reluctance in binding to Watson-Crick base pairs within double helix.

Rubin, J R, Sabat, M, Sundaralingam, M

A comparative study of the binding of square planar cis- and trans-[Pt(NH3)2Cl2] complexes and the octahedral [Ru(NH3)5(H2O)]3+ complex to tRNAphe from yeast was carried out by X-ray crystallography....

Crystal structure of the highly distorted chimeric decamer r(C)d(CGGCGCCG)r(G).spermine complex--spermine binding to phosphate only and minor groove tertiary base-pairing.

Ban, C, Ramakrishnan, B, Sundaralingam, M

The crystal structure of the self-complementary chimeric decamer duplex r(C)d(CGGCGCCG)r(G), with RNA base pairs at both termini, has been solved at 1.9 A resolution by the molecular replacement...

Crystal and molecular structure of the alternating dodecamer d(GCGTACGTACGC) in the A-DNA form: comparison with the isomorphous non-alternating dodecamer d(CCGTACGTACGG).

Bingman, C, Jain, S, Zon, G, Sundaralingam, M

The crystal structure of the alternating dodecamer d(GCGTACGTACGC) (5'-GC) has been determined to a resolution of 2.55A using oscillation film data. The crystals belong to space group P6(1) 22, a = b...

Zwitterionic character of nucleotides: possible significance in the evolution of nucleic acids.

Sundaralingam, M, Prusiner, P

X-ray crystallography has shown that the free acids of adenosine 5'- and 3'-monophosphates and of cytidine 5'- and 3'-monophosphates exist as zwiterions in the solid state with protonation of the...

Stacking of Crick Wobble pair and Watson-Crick pair: stability rules of G-U pairs at ends of helical stems in tRNAs and the relation to codon-anticodon Wobble interaction.

Mizuno, H, Sundaralingam, M

The occurrence of the noncomplementary G-U base pair at the end of a helix is found to be governed by stacking interactions. As a rule, a G-U pair with G on the 5'-side of a Watson-Crick base pair...

Analysis of the possible helical structures of nucleic acids and polynucleotides. Application of (n-h) plots.

Yathindra, N, Sundaralingam, M

The two helical parameters n and h where n is the number of nucleotide residues per turn and h is the height per nucleotide residue have been evaluated for single stranded helical polynucleotide...

Atomic coordinates and molecular conformation of yeast phenylalanyl tRNA. An independent investigation.

Stout, C D, Mizuno, H, Rubin, J, Brennan, T, Rao, S T, Sundaralingam, M

The atomic coordinates of yeast tRNA(Phe) in the monoclinic crystal form have been determined by an independent analysis from a model built into a 3 A MIR map. The overall molecular structure is...

Mechanisms of chain folding in nucleic acids. The (omega, omega) plot and its correlation to the nucleotide geometry in yeast tRNAPhe1.

Sundaralingam, M, Mizuno, H, Stout, C D, Rao, S T, Liedman, M, Yathindra, N

The (omega', omega) polot depicting the internucleotide P-O bond rotation angles in yeast phenylalanyl transfer RNA has established the interdependence of the phosphodiesters and the nucleotide...

Structure of transfer RNA molecules containing the long variable loop

Brennan, T., Sundaralingam, M.

A structure is proposed for the type II tRNA molecules containing the long variable loop and the tertiary base interactions here are compared with type I tRNAs having the short variable loop. The...

X-ray-structure of a cytidylyl-3',5'-adenosine-proflavine complex: a self-paired parallel-chain double helical dimer with an intercalated acridine dye.

Westhof, E, Sundaralingam, M

The non-self-complementary dinucleoside monophosphate cytidylyl-3',5'-adenosine (CpA) forms a base-paired parallel-chain dimer with an intercalated proflavine. The dimer complex possesses a...

Stabilization of the long central helix of troponin C by intrahelical salt bridges between charged amino acid side chains.

Sundaralingam, M, Drendel, W, Greaser, M

The unusual dumbbell shape of troponin C is due to the presence of a long alpha-helix of nine turns that connects the amino- and carboxyl-terminal calcium-binding domains. The center of the long...

Structures of the Visual Chromophores and Related Pigments: A Conformational Basis of Visual Excitation

Sundaralingam, M., Beddell, C.

A detailed conformational analysis of the known crystal structures of vitamin A, carotenoid, and xanthenoid derivatives has been made. These studies suggest that the visual chromophore 11-cis retinal...

Nonintercalative binding of ethidium bromide to nucleic acids: crystal structure of an ethidium--tRNA molecular complex.

Liebman, M, Rubin, J, Sundaralingam, M

X-ray diffraction studies at 4.5-A resolution on crystals of a complex of ethidium bromide and yeast phenylalanine tRNA reveal a nonintercalative model of binding of the ethidium within the tertiary...

Backbone Conformations in Secondary and Tertiary Structural Units of Nucleic Acids. Constraint in the Phosphodiester Conformation*

Yathindra, N., Sundaralingam, M.

The possible backbone phosphodiester conformations in a dinucleoside monophosphate and a dinucleoside triphosphate have been investigated by semiempirical energy calculations. Conformational energies...

Crystallographic analysis of the three-dimensional structure of baboon alpha-lactalbumin at low resolution. Homology with lysozyme.

Smith, S G, Lewis, M, Aschaffenburg, R, Fenna, R E, Wilson, I A, Sundaralingam, M, ...

The crystal structure of baboon alpha-lactalbumin has been determined at 6 A and at 4.5 A (0.6 nm and 0.45 nm) resolution by the method of isomorphous replacement. The principal derivative was...

Crystal structure of d(GCGCGCG) with 5'-overhang G residues.

Pan, B, Ban, C, Wahl, M C, Sundaralingam, M

The crystal structure of the DNA heptamer d(GCGCGCG) has been solved at 1.65 A resolution by the molecular replacement method and refined to an R-value of 0.184 for 3598 reflections. The heptamer...

Crystal structure of the B-DNA hexamer d(CTCGAG): model for an A-to-B transition.

Wahl, M C, Rao, S T, Sundaralingam, M

The crystal structure of the B-DNA hexamer d(CTCGAG) has been solved at 1.9 A resolution by iterative single isomorphous replacement, using the brominated derivative d(CG5BrCGAG), and refined to an...

Crystal structure of the self-complementary 5'-purine start decamer d(GCGCGCGCGC) in the Z-DNA conformation. I.

Ban, C, Ramakrishnan, B, Sundaralingam, M

Alternating self-complementary oligonucleotides starting with a 5'-pyrimidine usually form left-handed Z-DNA; however, with a 5'-purine start sequence they form the right-handed A-DNA. Here we report...

Crystal structure of the self-complementary 5'-purine start decamer d(GCACGCGTGC) in the A-DNA conformation. II.

Ban, C, Sundaralingam, M

The crystal structure of the alternating 5'-purine start decamer d(GCGCGCGCGC) was found to be in the left-handed Z-DNA conformation. Inasmuch as the A.T base pair is known to resist Z-DNA formation,...

Crystal structure of the A-DNA decamer d(CCIGGCCm5CGG) at 1.6 A showing the unexpected wobble I.m5C base pair.

Ramakrishnan, B, Sundaralingam, M

The crystal structure of the self-complementary decamer d(CCIGGCCm5CGG), where I and m5C replace A and T, respectively, in the Watson-Crick B-DNA decamer d(CCAGGCCTGG) is in the A-DNA conformation....

Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge.

Xiong, Y, Sundaralingam, M

The crystal structure of r(GCCACCCUG).r(CAGGGUCGGC), helix II of the Xenopus laevis 5S rRNA with a cytosine bulge (underlined), has been determined in two forms at 2.2 A (Form I, space group...

Crystal structures of two forms of a 14-mer RNA/DNA chimer duplex with double UU bulges: a novel intramolecular U*(A x U) base triple.

Deng, J, Xiong, Y, Sudarsanakumar, C, Shi, K, Sundaralingam, M

The RNA/DNA 14-mer, (gguauuucgguaCc)2 with consecutive uridine bulges (underlined) on each strand has been determined in two crystal forms, spermine bound (Sp-form) and spermine free (Sp-free). The...

A novel end-to-end binding of two netropsins to the DNA decamers d(CCCCCIIIII)2, d(CCCBr5CCIIIII)2and d(CBr5CCCCIIIII)2.

Chen, X, Mitra, S N, Rao, S T, Sekar, K, Sundaralingam, M

Netropsin is bound to the DNA decamer d(CCCCCIIIII)2, the C-4 bromo derivative d(CCCBr5CCIIIII)2and the C-2 bromo derivative d(CBr5CCCCIIIII)2in a novel 2:1 mode. Complexes of the native decamer and...

Crystal structure of an RNA octamer duplex r(CCCIUGGG)2 incorporating tandem I.U wobbles.

Pan, B, Mitra, S N, Sun, L, Hart, D, Sundaralingam, M

The crystal structure of the RNA octamer duplex r(CCCIUGGG)2has been elucidated at 2.5 A resolution. The crystals belong to the space group P21and have unit cell constants a = 33.44 A, b = 43.41 A, c...

Crystal structure of an RNA duplex r(G GCGC CC)2 with non-adjacent G*U base pairs.

Shi, K, Wahl, M, Sundaralingam, M

The crystal structure of a self-complementary RNA duplex r(GGGCGCUCC)2with non-adjacent G*U and U*G wobble pairs separated by four Watson-Crick base pairs has been determined to 2.5 A resolution....

STEREOCHEMISTRY OF NUCLEIC ACIDS AND THEIR CONSTITUENTS, XI. THE CONFORMATION OF THE ANTIBIOTIC PUROMYCIN DIHYDROCHLORIDE PENTAHYDRATE*†

Sundaralingam, M., Arora, S. K.

The elucidation of puromycin structure represents the first case where both a nucleic acid and a protein fragment are found together in a crystal. The molecule displays an elongated conformation with...

Biological Membrane Structure, II. A Detailed Model for the Retinal Rod Outer Segment Membrane

Vanderkooi, G., Sundaralingam, M.

A model, based on the available chemical, electron microscopic, and x-ray diffraction data, has been developed for the structure of the retinal rod outer segment membranes. The model consists of a...

X-ray structure of cytidine-5'-O-dimethylphosphate. Novel stacking between the ribosyl O(2') hydroxyl oxygen atom and the base.

Brennan, R G, Kondo, N S, Sundaralingam, M

The anionic oxygen atoms of the phosphodiester backbone of RNA and DNA are particularly susceptible to esterification by many mutagenic and carcinogenic alkylating agents. To better understand the...

Similar binding of the carcinostatic drugs cis-[Pt(NH3)2Cl2] and [Ru(NH3)5Cl] Cl2 to tRNAphe and a comparison with the binding of the inactive trans-[Pt(NH3)2Cl2] complex - reluctance in binding to Watson-Crick base pairs within double helix.

Rubin, J R, Sabat, M, Sundaralingam, M

A comparative study of the binding of square planar cis- and trans-[Pt(NH3)2Cl2] complexes and the octahedral [Ru(NH3)5(H2O)]3+ complex to tRNAphe from yeast was carried out by X-ray crystallography....

Crystal structure of the highly distorted chimeric decamer r(C)d(CGGCGCCG)r(G).spermine complex--spermine binding to phosphate only and minor groove tertiary base-pairing.

Ban, C, Ramakrishnan, B, Sundaralingam, M

The crystal structure of the self-complementary chimeric decamer duplex r(C)d(CGGCGCCG)r(G), with RNA base pairs at both termini, has been solved at 1.9 A resolution by the molecular replacement...

Crystal and molecular structure of the alternating dodecamer d(GCGTACGTACGC) in the A-DNA form: comparison with the isomorphous non-alternating dodecamer d(CCGTACGTACGG).

Bingman, C, Jain, S, Zon, G, Sundaralingam, M

The crystal structure of the alternating dodecamer d(GCGTACGTACGC) (5'-GC) has been determined to a resolution of 2.55A using oscillation film data. The crystals belong to space group P6(1) 22, a = b...

Zwitterionic character of nucleotides: possible significance in the evolution of nucleic acids.

Sundaralingam, M, Prusiner, P

X-ray crystallography has shown that the free acids of adenosine 5'- and 3'-monophosphates and of cytidine 5'- and 3'-monophosphates exist as zwiterions in the solid state with protonation of the...

Stacking of Crick Wobble pair and Watson-Crick pair: stability rules of G-U pairs at ends of helical stems in tRNAs and the relation to codon-anticodon Wobble interaction.

Mizuno, H, Sundaralingam, M

The occurrence of the noncomplementary G-U base pair at the end of a helix is found to be governed by stacking interactions. As a rule, a G-U pair with G on the 5'-side of a Watson-Crick base pair...

Analysis of the possible helical structures of nucleic acids and polynucleotides. Application of (n-h) plots.

Yathindra, N, Sundaralingam, M

The two helical parameters n and h where n is the number of nucleotide residues per turn and h is the height per nucleotide residue have been evaluated for single stranded helical polynucleotide...

Atomic coordinates and molecular conformation of yeast phenylalanyl tRNA. An independent investigation.

Stout, C D, Mizuno, H, Rubin, J, Brennan, T, Rao, S T, Sundaralingam, M

The atomic coordinates of yeast tRNA(Phe) in the monoclinic crystal form have been determined by an independent analysis from a model built into a 3 A MIR map. The overall molecular structure is...

Mechanisms of chain folding in nucleic acids. The (omega, omega) plot and its correlation to the nucleotide geometry in yeast tRNAPhe1.

Sundaralingam, M, Mizuno, H, Stout, C D, Rao, S T, Liedman, M, Yathindra, N

The (omega', omega) polot depicting the internucleotide P-O bond rotation angles in yeast phenylalanyl transfer RNA has established the interdependence of the phosphodiesters and the nucleotide...

Structure of transfer RNA molecules containing the long variable loop

Brennan, T., Sundaralingam, M.

A structure is proposed for the type II tRNA molecules containing the long variable loop and the tertiary base interactions here are compared with type I tRNAs having the short variable loop. The...

X-ray-structure of a cytidylyl-3',5'-adenosine-proflavine complex: a self-paired parallel-chain double helical dimer with an intercalated acridine dye.

Westhof, E, Sundaralingam, M

The non-self-complementary dinucleoside monophosphate cytidylyl-3',5'-adenosine (CpA) forms a base-paired parallel-chain dimer with an intercalated proflavine. The dimer complex possesses a...

Stabilization of the long central helix of troponin C by intrahelical salt bridges between charged amino acid side chains.

Sundaralingam, M, Drendel, W, Greaser, M

The unusual dumbbell shape of troponin C is due to the presence of a long alpha-helix of nine turns that connects the amino- and carboxyl-terminal calcium-binding domains. The center of the long...

Structures of the Visual Chromophores and Related Pigments: A Conformational Basis of Visual Excitation

Sundaralingam, M., Beddell, C.

A detailed conformational analysis of the known crystal structures of vitamin A, carotenoid, and xanthenoid derivatives has been made. These studies suggest that the visual chromophore 11-cis retinal...

Nonintercalative binding of ethidium bromide to nucleic acids: crystal structure of an ethidium--tRNA molecular complex.

Liebman, M, Rubin, J, Sundaralingam, M

X-ray diffraction studies at 4.5-A resolution on crystals of a complex of ethidium bromide and yeast phenylalanine tRNA reveal a nonintercalative model of binding of the ethidium within the tertiary...

Backbone Conformations in Secondary and Tertiary Structural Units of Nucleic Acids. Constraint in the Phosphodiester Conformation*

Yathindra, N., Sundaralingam, M.

The possible backbone phosphodiester conformations in a dinucleoside monophosphate and a dinucleoside triphosphate have been investigated by semiempirical energy calculations. Conformational energies...

Crystallographic analysis of the three-dimensional structure of baboon alpha-lactalbumin at low resolution. Homology with lysozyme.

Smith, S G, Lewis, M, Aschaffenburg, R, Fenna, R E, Wilson, I A, Sundaralingam, M, ...

The crystal structure of baboon alpha-lactalbumin has been determined at 6 A and at 4.5 A (0.6 nm and 0.45 nm) resolution by the method of isomorphous replacement. The principal derivative was...

Crystal structure of d(GCGCGCG) with 5'-overhang G residues.

Pan, B, Ban, C, Wahl, M C, Sundaralingam, M

The crystal structure of the DNA heptamer d(GCGCGCG) has been solved at 1.65 A resolution by the molecular replacement method and refined to an R-value of 0.184 for 3598 reflections. The heptamer...

Crystal structure of the B-DNA hexamer d(CTCGAG): model for an A-to-B transition.

Wahl, M C, Rao, S T, Sundaralingam, M

The crystal structure of the B-DNA hexamer d(CTCGAG) has been solved at 1.9 A resolution by iterative single isomorphous replacement, using the brominated derivative d(CG5BrCGAG), and refined to an...

Crystal structure of the self-complementary 5'-purine start decamer d(GCGCGCGCGC) in the Z-DNA conformation. I.

Ban, C, Ramakrishnan, B, Sundaralingam, M

Alternating self-complementary oligonucleotides starting with a 5'-pyrimidine usually form left-handed Z-DNA; however, with a 5'-purine start sequence they form the right-handed A-DNA. Here we report...

Crystal structure of the self-complementary 5'-purine start decamer d(GCACGCGTGC) in the A-DNA conformation. II.

Ban, C, Sundaralingam, M

The crystal structure of the alternating 5'-purine start decamer d(GCGCGCGCGC) was found to be in the left-handed Z-DNA conformation. Inasmuch as the A.T base pair is known to resist Z-DNA formation,...

Crystal structure of the A-DNA decamer d(CCIGGCCm5CGG) at 1.6 A showing the unexpected wobble I.m5C base pair.

Ramakrishnan, B, Sundaralingam, M

The crystal structure of the self-complementary decamer d(CCIGGCCm5CGG), where I and m5C replace A and T, respectively, in the Watson-Crick B-DNA decamer d(CCAGGCCTGG) is in the A-DNA conformation....

Two crystal forms of helix II of Xenopus laevis 5S rRNA with a cytosine bulge.

Xiong, Y, Sundaralingam, M

The crystal structure of r(GCCACCCUG).r(CAGGGUCGGC), helix II of the Xenopus laevis 5S rRNA with a cytosine bulge (underlined), has been determined in two forms at 2.2 A (Form I, space group...

Crystal structures of two forms of a 14-mer RNA/DNA chimer duplex with double UU bulges: a novel intramolecular U*(A x U) base triple.

Deng, J, Xiong, Y, Sudarsanakumar, C, Shi, K, Sundaralingam, M

The RNA/DNA 14-mer, (gguauuucgguaCc)2 with consecutive uridine bulges (underlined) on each strand has been determined in two crystal forms, spermine bound (Sp-form) and spermine free (Sp-free). The...

Crystal structure of the Y52F/Y73F double mutant of phospholipase A2: increased hydrophobic interactions of the phenyl groups compensate for the disrupted hydrogen bonds of the tyrosines.

Sekharudu, C., Ramakrishnan, B., Huang, B., Jiang, R. T., Dupureur, C. M., Tsai, M. D., ...

The enzyme phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 ester bond of membrane phospholipids. The highly conserved Tyr residues 52 and 73 in the enzyme form hydrogen bonds to the...

A model for the calmodulin-peptide complex based on the troponin C crystal packing and its similarity to the NMR structure of the calmodulin-myosin light chain kinase peptide complex.

Sekharudu, C. Y., Sundaralingam, M.

In the crystal structure of troponin C, the holo C-domain is bound in a head-to-tail fashion to the A-helix of the apo N-domain of a symmetry-related molecule. Using this interaction, we have...

Structure of Paramecium tetraurelia calmodulin at 1.8 A resolution.

Rao, S. T., Wu, S., Satyshur, K. A., Ling, K. Y., Kung, C., Sundaralingam, M.

The crystal structure of calmodulin (CaM; M(r) 16,700, 148 residues) from the ciliated protozoan Paramecium tetraurelia (PCaM) has been determined and refined using 1.8 A resolution area detector...

Structure and function of the catalytic site mutant Asp 99 Asn of phospholipase A2: absence of the conserved structural water.

Kumar, A., Sekharudu, C., Ramakrishnan, B., Dupureur, C. M., Zhu, H., Tsai, M. D., ...

To probe the role of the Asp-99 ... His-48 pair in phospholipase A2 (PLA2) catalysis, the X-ray structure and kinetic characterization of the mutant Asp-99-->Asn-99 (D99N) of bovine pancreatic PLA2...