Shirasawa, Shin-ichi, Yoshimi, Masayuki, Kamochi, Hideaki, Muneta, Takeshi, Kimura, James H., Höök, Magnus, ...
We have developed a simple and unique strategy for identifying cell surface and extracellular matrix molecules produced by chondrocytes. Our strategy comprises two methods, retrovirus-based signal...
Schwarz-Linek, Ulrich, Werner, Jörn M., Pickford, Andrew R., Gurusiddappa, Sivashankarappa, Kim, Jung Hwa, Pilka, Ewa S., ...
Staphylococcus aureus and Streptococcus pyogenes, two important human pathogens, target host fibronectin (Fn) in their adhesion to and invasion of host cells1, 2. Fibronectin-binding proteins...
Hoke, David E, Carson, Daniel D, Höök, Magnus
Abstract Despite extensive progress in determining structures within heparin and heparan sulfate (Hp/HS) and the discovery of numerous proteinaceous binding partners for Hp/HS so far; the only...
Liu, Shouchun, Zhou, Fanyu, Höök, Magnus, Carson, Daniel D.
We have previously identified and characterized a heparin-binding cell surface protein (heparin/heparan sulfate-interacting protein, or HIP) present on epithelial and endothelial cells. A synthetic...
Characterization of the Collagen-Binding S-Layer Protein CbsA of Lactobacillus crispatus
Sillanpää, Jouko, Martínez, Beatriz, Antikainen, Jenni, Toba, Takahiro, Kalkkinen, Nisse, Tankka, Sanna, ...
The cbsA gene of Lactobacillus crispatus strain JCM 5810, encoding a protein that mediates adhesiveness to collagens, was characterized and expressed in Escherichia coli. The cbsA open reading frame...
Inhibition of Staphylococcus aureus Adherence to Collagen under Dynamic Conditions
Mohamed, Nehal, Teeters, Mark A., Patti, Joseph M., Höök, Magnus, Ross, Julia M.
Staphylococcus aureus is the most common etiological agent of bacterial arthritis and acute osteomyelitis and has been shown to bind to type II collagen under static and dynamic conditions. We have...
The Collagen-Binding Adhesin Is a Virulence Factor in Staphylococcus aureus Keratitis
Rhem, Marcus N., Lech, Elizabeth M., Patti, Joseph M., McDevitt, Damien, Höök, Magnus, Jones, Dan B., ...
A collagen-binding strain of Staphylococcus aureus produced suppurative inflammation in a rabbit model of soft contact lens-associated bacterial keratitis more often than its...
Mapping the Ligand-Binding Region of Borrelia burgdorferi Fibronectin-Binding Protein BBK32
Probert, William S., Kim, Jung Hwa, Höök, Magnus, Johnson, Barbara J. B.
The cellular attachment and entry of pathogenic microorganisms can be facilitated by the expression of microbial adhesins that bind fibronectin. We have previously described a Borrelia burgdorferi...
Nallapareddy, Sreedhar R., Qin, Xiang, Weinstock, George M., Höök, Magnus, Murray, Barbara E.
Adhesin-mediated binding to extracellular matrix (ECM) proteins is thought to be a crucial step in the pathogenic process of many bacterial infections. We have previously reported conditional...
Hanson, Mark S., Cassatt, David R., Guo, Betty P., Patel, Nita K., McCarthy, Michael P., Dorward, David W., ...
Borrelia burgdorferi, the spirochete that causes Lyme disease, binds decorin, a collagen-associated extracellular matrix proteoglycan found in the skin (the site of entry for the spirochete) and in...
Casolini, Fabrizia, Visai, Livia, Joh, Danny, Conaldi, Pier Giulio, Toniolo, Antonio, Höök, Magnus, ...
We have analyzed antibody reactivity to a fibronectin-binding microbial surface component that recognizes adhesive matrix molecules (MSCRAMM) in blood plasma collected from patients with...
Deivanayagam, Champion C.S., Wann, Elisabeth R., Chen, Wei, Carson, Mike, Rajashankar, Kanagalaghatta R., Höök, Magnus, ...
We report here the crystal structure of the minimal ligand-binding segment of the Staphylococcus aureus MSCRAMM, clumping factor A. This fibrinogen-binding segment contains two similarly folded...
Simpson, Kathryn H., Bowden, Gabriela, Höök, Magnus, Anvari, Bahman
The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The...
Lee, Lawrence Y., Miyamoto, Yuko J., McIntyre, Bradley W., Höök, Magnus, McCrea, Kirk W., McDevitt, Damien, ...
Staphylococcus aureus (SA) is an opportunistic pathogen that affects a variety of organ systems and is responsible for many diseases worldwide. SA express an MHC class II analog protein (Map), which...
Hoke, David E, Carson, Daniel D, Höök, Magnus
Despite extensive progress in determining structures within heparin and heparan sulfate (Hp/HS) and the discovery of numerous proteinaceous binding partners for Hp/HS so far; the only detailed...
Resistance to Lyme disease in decorin-deficient mice
Brown, Eric L., Wooten, R. Mark, Johnson, Barbara J.B., Iozzo, Renato V., Smith, Amanda, Dolan, Marc C., ...
Microbial adhesion to the host tissue represents an early, critical step in the pathogenesis of most infectious diseases. Borrelia burgdorferi, the causative agent of Lyme disease (LD), expresses two...
Interaction of lipoprotein lipase with native and modified heparin-like polysaccharides
Bengtsson, Gunilla, Olivecrona, Thomas, Höök, Magnus, Riesenfeld, Johan, Lindahl, Ulf
1. Lipoprotein lipase (EC 3.1.1.34), which was previously shown to bind to immobilized heparin, was now found to bind also to heparan sulphate and dermatan sulphate and to some extent to chondroitin...
Structure and metabolism of rat liver heparan sulphate
Oldberg, Åke, Höök, Magnus, Öbrink, Björn, Pertoft, Håkan, Rubin, Kristofer
Rat liver cells grown in primary cultures in the presence of [35S]sulphate synthesize a labelled heparan sulphate-like glycosaminoglycan. The characterization of the polysaccharide as heparan...
Distribution of sulphate and iduronic acid residues in heparin and heparan sulphate
Höök, Magnus, Lindahl, Ulf, Iverius, Per-Henrik
1. A method was developed for determination of the uronic acid composition of heparin-like glycosaminoglycans. Polymers or oligosaccharides are degraded to monosaccharides by a combination of acid...
Steiglitz, Barry M., Kreider, Jaclynn M., Frankenburg, Elizabeth P., Pappano, William N., Hoffman, Guy G., Meganck, Jeffrey A., ...
Procollagen C proteinases (pCPs) cleave type I to III procollagen C propeptides as a necessary step in assembling the major fibrous components of vertebrate extracellular matrix. The protein PCOLCE1...
Endocarditis and biofilm-associated pili of Enterococcus faecalis
Nallapareddy, Sreedhar R., Singh, Kavindra V., Sillanpää, Jouko, Garsin, Danielle A., Höök, Magnus, Erlandsen, Stanley L., ...
Increasing multidrug resistance in Enterococcus faecalis, a nosocomial opportunist and common cause of bacterial endocarditis, emphasizes the need for alternative therapeutic approaches such as...
A ‘Collagen Hug' Model for Staphylococcus aureus CNA binding to collagen
Zong, Yinong, Xu, Yi, Liang, Xiaowen, Keene, Douglas R, Höök, Agneta, Gurusiddappa, Shivasankarappa, ...
The structural basis for the association of eukaryotic and prokaryotic protein receptors and their triple-helical collagen ligand remains poorly understood. Here, we present the crystal structures of...
Liu, Shouchun, Zhou, Fanyu, Höök, Magnus, Carson, Daniel D.
We have previously identified and characterized a heparin-binding cell surface protein (heparin/heparan sulfate-interacting protein, or HIP) present on epithelial and endothelial cells. A synthetic...
Characterization of the Collagen-Binding S-Layer Protein CbsA of Lactobacillus crispatus
Sillanpää, Jouko, Martínez, Beatriz, Antikainen, Jenni, Toba, Takahiro, Kalkkinen, Nisse, Tankka, Sanna, ...
The cbsA gene of Lactobacillus crispatus strain JCM 5810, encoding a protein that mediates adhesiveness to collagens, was characterized and expressed in Escherichia coli. The cbsA open reading frame...
Inhibition of Staphylococcus aureus Adherence to Collagen under Dynamic Conditions
Mohamed, Nehal, Teeters, Mark A., Patti, Joseph M., Höök, Magnus, Ross, Julia M.
Staphylococcus aureus is the most common etiological agent of bacterial arthritis and acute osteomyelitis and has been shown to bind to type II collagen under static and dynamic conditions. We have...
The Collagen-Binding Adhesin Is a Virulence Factor in Staphylococcus aureus Keratitis
Rhem, Marcus N., Lech, Elizabeth M., Patti, Joseph M., McDevitt, Damien, Höök, Magnus, Jones, Dan B., ...
A collagen-binding strain of Staphylococcus aureus produced suppurative inflammation in a rabbit model of soft contact lens-associated bacterial keratitis more often than its...
Mapping the Ligand-Binding Region of Borrelia burgdorferi Fibronectin-Binding Protein BBK32
Probert, William S., Kim, Jung Hwa, Höök, Magnus, Johnson, Barbara J. B.
The cellular attachment and entry of pathogenic microorganisms can be facilitated by the expression of microbial adhesins that bind fibronectin. We have previously described a Borrelia burgdorferi...
Nallapareddy, Sreedhar R., Qin, Xiang, Weinstock, George M., Höök, Magnus, Murray, Barbara E.
Adhesin-mediated binding to extracellular matrix (ECM) proteins is thought to be a crucial step in the pathogenic process of many bacterial infections. We have previously reported conditional...
Hanson, Mark S., Cassatt, David R., Guo, Betty P., Patel, Nita K., McCarthy, Michael P., Dorward, David W., ...
Borrelia burgdorferi, the spirochete that causes Lyme disease, binds decorin, a collagen-associated extracellular matrix proteoglycan found in the skin (the site of entry for the spirochete) and in...
Casolini, Fabrizia, Visai, Livia, Joh, Danny, Conaldi, Pier Giulio, Toniolo, Antonio, Höök, Magnus, ...
We have analyzed antibody reactivity to a fibronectin-binding microbial surface component that recognizes adhesive matrix molecules (MSCRAMM) in blood plasma collected from patients with...
Deivanayagam, Champion C.S., Wann, Elisabeth R., Chen, Wei, Carson, Mike, Rajashankar, Kanagalaghatta R., Höök, Magnus, ...
We report here the crystal structure of the minimal ligand-binding segment of the Staphylococcus aureus MSCRAMM, clumping factor A. This fibrinogen-binding segment contains two similarly folded...
Simpson, Kathryn H., Bowden, Gabriela, Höök, Magnus, Anvari, Bahman
The force required to rupture bonds between individual Staphylococcus aureus MSCRAMMs and surfaces coated with extracellular matrix molecules has been quantified by using optical tweezers. The...
Lee, Lawrence Y., Miyamoto, Yuko J., McIntyre, Bradley W., Höök, Magnus, McCrea, Kirk W., McDevitt, Damien, ...
Staphylococcus aureus (SA) is an opportunistic pathogen that affects a variety of organ systems and is responsible for many diseases worldwide. SA express an MHC class II analog protein (Map), which...
Hoke, David E, Carson, Daniel D, Höök, Magnus
Despite extensive progress in determining structures within heparin and heparan sulfate (Hp/HS) and the discovery of numerous proteinaceous binding partners for Hp/HS so far; the only detailed...
Resistance to Lyme disease in decorin-deficient mice
Brown, Eric L., Wooten, R. Mark, Johnson, Barbara J.B., Iozzo, Renato V., Smith, Amanda, Dolan, Marc C., ...
Microbial adhesion to the host tissue represents an early, critical step in the pathogenesis of most infectious diseases. Borrelia burgdorferi, the causative agent of Lyme disease (LD), expresses two...
Interaction of lipoprotein lipase with native and modified heparin-like polysaccharides
Bengtsson, Gunilla, Olivecrona, Thomas, Höök, Magnus, Riesenfeld, Johan, Lindahl, Ulf
1. Lipoprotein lipase (EC 3.1.1.34), which was previously shown to bind to immobilized heparin, was now found to bind also to heparan sulphate and dermatan sulphate and to some extent to chondroitin...
Structure and metabolism of rat liver heparan sulphate
Oldberg, Åke, Höök, Magnus, Öbrink, Björn, Pertoft, Håkan, Rubin, Kristofer
Rat liver cells grown in primary cultures in the presence of [35S]sulphate synthesize a labelled heparan sulphate-like glycosaminoglycan. The characterization of the polysaccharide as heparan...
Distribution of sulphate and iduronic acid residues in heparin and heparan sulphate
Höök, Magnus, Lindahl, Ulf, Iverius, Per-Henrik
1. A method was developed for determination of the uronic acid composition of heparin-like glycosaminoglycans. Polymers or oligosaccharides are degraded to monosaccharides by a combination of acid...
Steiglitz, Barry M., Kreider, Jaclynn M., Frankenburg, Elizabeth P., Pappano, William N., Hoffman, Guy G., Meganck, Jeffrey A., ...
Procollagen C proteinases (pCPs) cleave type I to III procollagen C propeptides as a necessary step in assembling the major fibrous components of vertebrate extracellular matrix. The protein PCOLCE1...
Endocarditis and biofilm-associated pili of Enterococcus faecalis
Nallapareddy, Sreedhar R., Singh, Kavindra V., Sillanpää, Jouko, Garsin, Danielle A., Höök, Magnus, Erlandsen, Stanley L., ...
Increasing multidrug resistance in Enterococcus faecalis, a nosocomial opportunist and common cause of bacterial endocarditis, emphasizes the need for alternative therapeutic approaches such as...
A ‘Collagen Hug' model for Staphylococcus aureus CNA binding to collagen
Zong, Yinong, Xu, Yi, Liang, Xiaowen, Keene, Douglas R, Höök, Agneta, Gurusiddappa, Shivasankarappa, ...
Correction to: The EMBO Journal (2005) 24, 4224–4236. doi:10.1038/sj.emboj.7600888
Nallapareddy, Sreedhar R., Sillanpää, Jouko, Ganesh, Vannakambadi K., Höök, Magnus, Murray, Barbara E.
Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and...
Bix, Gregory, Fu, Jian, Gonzalez, Eva M., Macro, Laura, Barker, Amy, Campbell, Shelly, ...
Endorepellin, the COOH-terminal domain of the heparan sulfate proteoglycan perlecan, inhibits several aspects of angiogenesis. We provide evidence for a novel biological axis that links a soluble...
Ganesh, Vannakambadi K., Rivera, Jose J., Smeds, Emanuel, Ko, Ya-Ping, Bowden, M. Gabriela, Wann, Elisabeth R., ...
The fibrinogen (Fg) binding MSCRAMM Clumping factor A (ClfA) from Staphylococcus aureus interacts with the C-terminal region of the fibrinogen (Fg) γ-chain. ClfA is the major virulence factor...
Bingham, Richard J., Rudiño-Piñera, Enrique, Meenan, Nicola A. G., Schwarz-Linek, Ulrich, Turkenburg, Johan P., Höök, Magnus, ...
Staphylococcus aureus can adhere to and invade endothelial cells by binding to the human protein fibronectin (Fn). FnBPA and FnBPB, cell wall-attached proteins from S. aureus, have multiple,...
A Novel Fibronectin Binding Motif in MSCRAMMs Targets F3 Modules
Prabhakaran, Sabitha, Liang, Xiaowen, Skare, Jonathan T., Potts, Jennifer R., Höök, Magnus
Borrelia burgdorferi Lacking DbpBA Exhibits an Early Survival Defect during Experimental Infection▿
Weening, Eric H., Parveen, Nikhat, Trzeciakowski, Jerome P., Leong, John M., Höök, Magnus, Skare, Jonathan T.
Several Borrelia burgdorferi genes induced under mammalian host conditions have been purported to be important in Lyme disease pathogenesis based on their binding to host structures. These genes...