Yamada, Shuhei, Onishi, Masako, Fujinawa, Reiko, Tadokoro, Yuko, Okabayashi, Koji, Asashima, Makoto, ...
Xenopus laevis is an excellent animal for analyzing early vertebrate development. Various effects of glycosaminoglycans (GAGs) on growth factor-related cellular events during embryogenesis have been...
Global Expression of Simulated Microgravity-Responsive Genes in Xenopus Liver Cells (2008)
Masayuki Ikuzawa, Makoto Asashima
The random positioning machine (RPM) is a method used to generate a simulated-microgravity environment at approximately 0 g. Using an RPM, we analyzed the global gene expression of A8 cells derived...
Yasuko Onuma, Akira Watanabe, Hiroyuki Aburatani, Makoto Asashima, Malcolm Whitman
Here we report a novel small protein that is highly conserved across vertebrates. The protein, which we have named TRIQK, has no homology to any previously reported proteins or functional domains,...
Ohnuma, Kiyoshi, Yomo, Tetsuya, Asashima, Makoto, Kaneko, Kunihiko
Abstract Background Single-cell level studies are being used increasingly to measure cell properties not directly observable in a cell population. High-performance data acquisition systems for such...
Wada, Masahito, Miyazawa, Hiroshi, Wang, Rui-Sheng, Mizun, Takeshi, Sato, Akira, Asashima, Makoto, ...
DNA polymerase ϵ is essential for cell viability and chromosomal DNA replication in budding yeast. In addition, DNA polymerase ϵ may be involved in DNA repair and cell-cycle checkpoint...
ISOLEUCINE PREVENTS RAT SALIVARY GLAND EPITHELIAL CELLS FROM APOPTOSIS IN SERUM-FREE CULTURE (2000)
MIHO FURUE, TETSUJI OKAMOTO, SHINRI KOSHIKA, MAKOTO ASASHIMA
Takashi Ariizumi, Shinji Komazaki, Makoto Asashima
The inductive interactions between activin-induced and non-induced cells were investigated in newt animal cap explants. A wide range of concentrations of activin A (0.1–100 ng/ml) induced...
Takashi Ariizumi, Shinji Komazaki, Makoto Asashima
The inductive interactions between activin-induced and non-induced cells were investigated in newt animal cap explants. A wide range of concentrations of activin A (0.1–100 ng/ml) induced...
Naomi Moriya, Chika Yokota, Takashi Ariizumi, Makoto Asashima
We have demonstrated that the tissue differentiation patterns along the dorsoventral and anteroposterior axes can be controlled by a combination of activin A, concanavalin A (Con A), and retinoic...
Takashi Ariizumi, Kazuhiro Takano, Hiromasa Ninomiya, Makoto Asashima
The differentiation patterns of animal cap explants from the Japanese salamanders Hynobius lichenatus and Hynobius nigrescens were examined after exposure to various concentrations of activin A. A...
Naomi Moriya, Chika Yokota, Takashi Ariizumi, Makoto Asashima
We have demonstrated that the tissue differentiation patterns along the dorsoventral and anteroposterior axes can be controlled by a combination of activin A, concanavalin A (Con A), and retinoic...
Takashi Ariizumi, Kazuhiro Takano, Hiromasa Ninomiya, Makoto Asashima
The differentiation patterns of animal cap explants from the Japanese salamanders Hynobius lichenatus and Hynobius nigrescens were examined after exposure to various concentrations of activin A. A...
Regulation of the Xmyf-5 and XmyoD Expression Pattern during Early Xenopus Development (1998)
Shuji Takahashi, Eisaku Esumi, Yo-ichi Nabeshima, Makoto Asashima
In beginning of muscle development, determination is induced in the mesoderm, and then differentiation occurs with accumulation of muscle structural proteins. Mesoderm cells differentiate to many...
Midkine Counteracts the Activin Signal in Mesoderm Induction and Promotes Neural Formation (1998)
Yokota, Chika, Takahashi, Shuji, Eisaki, Akira, Asashima, Makoto, Akhter, Shamima, Muramatsu, Takashi, ...
Midkine (MK) is a heparin-binding growth factor that has been implicated in neural survival and differentiation, fibrinolysis, and carcinogenesis. It is expressed in the nervous system during early...
Control of the Embryonic Body Plan by Activin during Amphibian Development (1995)
Takashi Ariizumi, Makoto Asashima
Embryonic induction plays an important role in establishing the fundamental body plan during early amphibian development. The factors mediating this embryonic induction have, however, only recently...
Expression of BMP-2,4 Genes during Early Development in Xenopus (1995)
Toshiyuki Yamagishi, Shin-ichiro Nishimatsu, Shintaro Nomura, Makoto Asashima, Kazuo Murakami, Naoto Ueno
The transforming growth factor-β (TGF-β) family has been implicated in the regulation of cell differentiation during early development. Bone morphogenetic proteins (BMPs) are thought to play...
Restricted Expression of Xenopus Midkine Gene during Early Development (1995)
Sekiguchi, Kaneo, Yokota, Chika, Asashima, Makoto, Kaname, Tadashi, Fan, Qi-Wen, Muramatsu, Takashi, ...
Midkine (MK) is a heparin-binding growth factor and forms a novel protein family together with another member, pleiotrophin (PTN)/heparin-binding growth-associated molecule (HB-GAM). A cDNA clone...
Kato, Ryoichi, Kamada, Hiroshi, Asashima, Makoto
Hairy roots of Daucus carota and Atropa belladonna, which were induced by inoculation with Agrobacterium rhizogenes that harbored the Ri plasmid, were cultured on Murashige and Skoog's solid medium...
In vitro organogenesis using amphibian pluripotential cells
Asashima, Makoto, Okabayashi, K.
In the embryonic development of vertebrate, sequential cleavage is followed by the gastrulation as the first dynamic event of morphogenesis. Mesodermal induction is the most important event for...
Inhibition of the Wnt Signaling Pathway by Idax, a Novel Dvl-Binding Protein
Hino, Shin-ichiro, Kishida, Shosei, Michiue, Tatsuo, Fukui, Akimasa, Sakamoto, Ikuo, Takada, Shinji, ...
In attempting to clarify the roles of Dvl in the Wnt signaling pathway, we identified a novel protein which binds to the PDZ domain of Dvl and named it Idax (for inhibition of the Dvl and Axin...
Yamamoto, Hideki, Kishida, Shosei, Uochi, Takaaki, Ikeda, Satoshi, Koyama, Shinya, Asashima, Makoto, ...
Using a yeast two-hybrid method, we identified a novel protein which interacts with glycogen synthase kinase 3β (GSK-3β). This protein had 44% amino acid identity with Axin, a negative regulator of...
Onuma, Yasuko, Takahashi, Shuji, Asashima, Makoto, Kurata, Shoichiro, Gehring, Walter J.
Loss of Pax 6 function leads to an eyeless phenotype in both mammals and insects, and ectopic expression of both the Drosophila and the mouse gene leads to the induction of ectopic eyes in...
Kadoya, Takayuki, Yamamoto, Hideki, Suzuki, Toshiaki, Yukita, Akira, Fukui, Akimasa, Michiue, Tatsuo, ...
Axam has been identified as a novel Axin-binding protein that inhibits the Wnt signaling pathway. We studied the molecular mechanism by which Axam stimulates the downregulation of β-catenin. The...
Activin A induces craniofacial cartilage from undifferentiated Xenopus ectoderm in vitro
Furue, Miho, Myoishi, Yasufumi, Fukui, Yasuto, Ariizumi, Takashi, Okamoto, Tetsuji, Asashima, Makoto
Activin A has potent mesoderm-inducing activity in amphibian embryos and induces various mesodermal tissues in vitro from the isolated presumptive ectoderm. By using a sandwich culture method...
Zinc Finger Protein Sall2 Is Not Essential for Embryonic and Kidney Development
Sato, Akira, Matsumoto, Yuko, Koide, Urara, Kataoka, Yuki, Yoshida, Nobuaki, Yokota, Takashi, ...
SALL/Sall is a mammalian homolog of the Drosophila region-specific homeotic gene spalt (sal), and heterozygous mutations in SALL1 in humans lead to Townes-Brocks syndrome. We earlier reported that...
Inhibition of the Wnt Signaling Pathway by Idax, a Novel Dvl-Binding Protein
Hino, Shin-ichiro, Kishida, Shosei, Michiue, Tatsuo, Fukui, Akimasa, Sakamoto, Ikuo, Takada, Shinji, ...
In attempting to clarify the roles of Dvl in the Wnt signaling pathway, we identified a novel protein which binds to the PDZ domain of Dvl and named it Idax (for inhibition of the Dvl and Axin...
Yamamoto, Hideki, Kishida, Shosei, Uochi, Takaaki, Ikeda, Satoshi, Koyama, Shinya, Asashima, Makoto, ...
Using a yeast two-hybrid method, we identified a novel protein which interacts with glycogen synthase kinase 3β (GSK-3β). This protein had 44% amino acid identity with Axin, a negative regulator of...
Onuma, Yasuko, Takahashi, Shuji, Asashima, Makoto, Kurata, Shoichiro, Gehring, Walter J.
Loss of Pax 6 function leads to an eyeless phenotype in both mammals and insects, and ectopic expression of both the Drosophila and the mouse gene leads to the induction of ectopic eyes in...
Kadoya, Takayuki, Yamamoto, Hideki, Suzuki, Toshiaki, Yukita, Akira, Fukui, Akimasa, Michiue, Tatsuo, ...
Axam has been identified as a novel Axin-binding protein that inhibits the Wnt signaling pathway. We studied the molecular mechanism by which Axam stimulates the downregulation of β-catenin. The...
Activin A induces craniofacial cartilage from undifferentiated Xenopus ectoderm in vitro
Furue, Miho, Myoishi, Yasufumi, Fukui, Yasuto, Ariizumi, Takashi, Okamoto, Tetsuji, Asashima, Makoto
Activin A has potent mesoderm-inducing activity in amphibian embryos and induces various mesodermal tissues in vitro from the isolated presumptive ectoderm. By using a sandwich culture method...
Zinc Finger Protein Sall2 Is Not Essential for Embryonic and Kidney Development
Sato, Akira, Matsumoto, Yuko, Koide, Urara, Kataoka, Yuki, Yoshida, Nobuaki, Yokota, Takashi, ...
SALL/Sall is a mammalian homolog of the Drosophila region-specific homeotic gene spalt (sal), and heterozygous mutations in SALL1 in humans lead to Townes-Brocks syndrome. We earlier reported that...
In vitro organogenesis using amphibian pluripotential cells
Asashima, Makoto, Okabayashi, K.
In the embryonic development of vertebrate, sequential cleavage is followed by the gastrulation as the first dynamic event of morphogenesis. Mesodermal induction is the most important event for...
Danno, Hiroki, Michiue, Tatsuo, Hitachi, Keisuke, Yukita, Akira, Ishiura, Shoichi, Asashima, Makoto
The neural-related genes Sox2, Pax6, Otx2, and Rax have been associated with severe ocular malformations such as anophthalmia and microphthalmia, but it remains unclear as to how these genes are...
IGFBP-4 is an inhibitor of canonical Wnt signalling required for cardiogenesis
Zhu, Weidong, Shiojima, Ichiro, 塩島, 一朗, Ito, Yuzuru, Li, Zhi, Ikeda, Hiroyuki, ...
Insulin-like growth factor binding proteins (IGFBPs) bind to and modulate the actionsof insulin-like growth factors (IGFs). Although some of the actions of IGFBPs havebeen reported to be independent...