A Mechanochemical Model for Mycoplasma Motility (2009)
Jing Chen, John Neu, Makoto Miyata, George Oster
The Mycoplasmas are a class of wall-less bacteria that are amongst the smallest free-living organisms. They move on surfaces using a novel, substrate-dependent gliding motility. The cell is driven by...
TAKIGUCHI, Hiromi, TSUBATA, Azusa, MIYATA, Makoto, ODAKE, Tamao, HOTTA, Hiroki, UMEMURA, Tomonari, ...
A flow injection analysis system was built with a liquid core waveguide spectrophotometric detector using an 80 cm Teflon AF-1600 capillary tube...
Miyata, Makoto, Sano, Ken-lchi, Okada, Ryosuke, Fukumura, Takashi
The homolog of the dnaA gene, which has been reported to be present in the vicinity of the initiation site of replication in the genome of Mycoplasma capricolum (M.Miyata, L.Wang, and T.Fukumura, J....
Miyata, Makoto, Arata, Toshiaki, Inoue, Akio
The bindings of S-1 and the two heads of HMM with pyrene-labeled F-actin were studied using the change in light-scattering intensity or that in the fluorescence intensity of the pyrenyl group. At low...
Miyata, Makoto, Arata, Toshiaki, Inoue, Akio
The species and amounts of intermediates formed by myosin in myofibrils during the ATPase reaction under relaxed conditions were examined. The amount of total nucleotides (ADP+ATP) bound to...
Reaction of Two Heads of Gizzard Myosin with ATP (1988)
Miyata, Makoto, Arata, Toshiaki, Inoue, Akio
The reaction intermediates formed by the two heads of smooth muscle myosin were studied. The amount of myosin-phosphate-ADP complex, MADPp, formed was measured from the P1-burst size over a wide...
Miyata, Makoto, Yasui, Mitsukuni, Arata, Toshiaki, Inoue, Akio
The rates of the elementary steps of the actomyosin ATPase reaction were measured using the myosin subfragment-1 of porcine left ventricular muscle. The results could be explained only by the...
Seto, Shintaro, Layh-Schmitt, Gerlinde, Kenri, Tsuyoshi, Miyata, Makoto
A method was developed for protein localization in Mycoplasma pneumoniae by immunofluorescence microscopy. The P1 adhesin protein was revealed to be located at least at one cell pole in all adhesive...
Partitioning, Movement, and Positioning of Nucleoids in Mycoplasma capricolum
Seto, Shintaro, Miyata, Makoto
The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4′,6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had...
Cell Reproduction and Morphological Changes in Mycoplasma capricolum
Seto, Shintaro, Miyata, Makoto
The cell reproduction of Mycoplasma capricolum was studied. The velocity of DNA replication fork progression was about 6 kb/min, which is 10 times slower than that of Escherichia coli. The time...
Force and Velocity of Mycoplasma mobile Gliding†
Miyata, Makoto, Ryu, William S., Berg, Howard C.
The effects of temperature and force on the gliding speed of Mycoplasma mobile were examined. Gliding speed increased linearly as a function of temperature from 0.46 μm/s at 11.5°C to 4.0 μm/s at...
Seto, Shintaro, Miyata, Makoto
Cytadherence proteins of Mycoplasma pneumoniae are localized at the attachment organelle, which is involved in adhesion, gliding motility, and cell division. The localization of these proteins in...
Uenoyama, Atsuko, Kusumoto, Akiko, Miyata, Makoto
Several mycoplasma species are known to glide in the direction of the membrane protrusion (head-like structure), but the mechanism underlying this movement is entirely unknown. To identify proteins...
Miyata, Makoto, Petersen, Jennifer D.
Mycoplasma mobile is a flask-shaped bacteria that binds to a substrate and glides towards its tapered end, the so-called “head-like protrusion,” by an unknown mechanism. To search for cellular...
Energetics of Gliding Motility in Mycoplasma mobile
Jaffe, Jacob D., Miyata, Makoto, Berg, Howard C.
Mycoplasma mobile glides on surfaces at up to 7 μm/s by an unknown mechanism. We studied the energetics that power gliding by using a novel, growth medium-free system. We found that cells could...
Kenri, Tsuyoshi, Seto, Shintaro, Horino, Atsuko, Sasaki, Yuko, Sasaki, Tsuguo, Miyata, Makoto
Mycoplasma pneumoniae lacks a cell wall but has internal cytoskeleton-like structures that are assumed to support the attachment organelle and asymmetric cell shape of this bacterium. To explore the...
Seto, Shintaro, Kenri, Tsuyoshi, Tomiyama, Tetsuo, Miyata, Makoto
To examine the participation of P1 adhesin in gliding of Mycoplasma pneumoniae, we examined the effects of an anti-P1 monoclonal antibody on individual gliding mycoplasmas. The antibody reduced the...
Seto, Shintaro, Uenoyama, Atsuko, Miyata, Makoto
Several mycoplasma species are known to glide on solid surfaces such as glass in the direction of the membrane protrusion, but the mechanism underlying this movement is unknown. To identify a novel...
Gliding ghosts of Mycoplasma mobile
Uenoyama, Atsuko, Miyata, Makoto
Several species of mycoplasmas glide on solid surfaces, in the direction of their membrane protrusion at a cell pole, by an unknown mechanism. Our recent studies on the fastest species, Mycoplasma...
Uenoyama, Atsuko, Miyata, Makoto
Mycoplasma mobile glides on a glass surface in the direction of its tapered end by an unknown mechanism. Two large proteins, Gli349 and Gli521, were recently reported to be involved in glass binding...
Adan-Kubo, Jun, Uenoyama, Atsuko, Arata, Toshiaki, Miyata, Makoto
Several species of mycoplasmas rely on an unknown mechanism to glide across solid surfaces in the direction of a membrane protrusion at the cell pole. Our recent studies on the fastest species,...
Seto, Shintaro, Layh-Schmitt, Gerlinde, Kenri, Tsuyoshi, Miyata, Makoto
A method was developed for protein localization in Mycoplasma pneumoniae by immunofluorescence microscopy. The P1 adhesin protein was revealed to be located at least at one cell pole in all adhesive...
Partitioning, Movement, and Positioning of Nucleoids in Mycoplasma capricolum
Seto, Shintaro, Miyata, Makoto
The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4′,6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had...
Cell Reproduction and Morphological Changes in Mycoplasma capricolum
Seto, Shintaro, Miyata, Makoto
The cell reproduction of Mycoplasma capricolum was studied. The velocity of DNA replication fork progression was about 6 kb/min, which is 10 times slower than that of Escherichia coli. The time...
Force and Velocity of Mycoplasma mobile Gliding†
Miyata, Makoto, Ryu, William S., Berg, Howard C.
The effects of temperature and force on the gliding speed of Mycoplasma mobile were examined. Gliding speed increased linearly as a function of temperature from 0.46 μm/s at 11.5°C to 4.0 μm/s at...
Seto, Shintaro, Miyata, Makoto
Cytadherence proteins of Mycoplasma pneumoniae are localized at the attachment organelle, which is involved in adhesion, gliding motility, and cell division. The localization of these proteins in...
Uenoyama, Atsuko, Kusumoto, Akiko, Miyata, Makoto
Several mycoplasma species are known to glide in the direction of the membrane protrusion (head-like structure), but the mechanism underlying this movement is entirely unknown. To identify proteins...
Miyata, Makoto, Petersen, Jennifer D.
Mycoplasma mobile is a flask-shaped bacteria that binds to a substrate and glides towards its tapered end, the so-called “head-like protrusion,” by an unknown mechanism. To search for cellular...
Energetics of Gliding Motility in Mycoplasma mobile
Jaffe, Jacob D., Miyata, Makoto, Berg, Howard C.
Mycoplasma mobile glides on surfaces at up to 7 μm/s by an unknown mechanism. We studied the energetics that power gliding by using a novel, growth medium-free system. We found that cells could...
Kenri, Tsuyoshi, Seto, Shintaro, Horino, Atsuko, Sasaki, Yuko, Sasaki, Tsuguo, Miyata, Makoto
Mycoplasma pneumoniae lacks a cell wall but has internal cytoskeleton-like structures that are assumed to support the attachment organelle and asymmetric cell shape of this bacterium. To explore the...
Seto, Shintaro, Kenri, Tsuyoshi, Tomiyama, Tetsuo, Miyata, Makoto
To examine the participation of P1 adhesin in gliding of Mycoplasma pneumoniae, we examined the effects of an anti-P1 monoclonal antibody on individual gliding mycoplasmas. The antibody reduced the...
Seto, Shintaro, Uenoyama, Atsuko, Miyata, Makoto
Several mycoplasma species are known to glide on solid surfaces such as glass in the direction of the membrane protrusion, but the mechanism underlying this movement is unknown. To identify a novel...
Gliding ghosts of Mycoplasma mobile
Uenoyama, Atsuko, Miyata, Makoto
Several species of mycoplasmas glide on solid surfaces, in the direction of their membrane protrusion at a cell pole, by an unknown mechanism. Our recent studies on the fastest species, Mycoplasma...
Uenoyama, Atsuko, Miyata, Makoto
Mycoplasma mobile glides on a glass surface in the direction of its tapered end by an unknown mechanism. Two large proteins, Gli349 and Gli521, were recently reported to be involved in glass binding...
Adan-Kubo, Jun, Uenoyama, Atsuko, Arata, Toshiaki, Miyata, Makoto
Several species of mycoplasmas rely on an unknown mechanism to glide across solid surfaces in the direction of a membrane protrusion at the cell pole. Our recent studies on the fastest species,...
Nagai, Ryoichiro, Miyata, Makoto
Mycoplasma mobile relies on an unknown mechanism to glide across solid surfaces including glass, animal cells, and plastics. To identify the direct binding target, we examined the factors that affect...
A microrotary motor powered by bacteria
Hiratsuka, Yuichi, Miyata, Makoto, Tada, Tetsuya, Uyeda, Taro Q. P.
Biological molecular motors have a number of unique advantages over artificial motors, including efficient conversion of chemical energy into mechanical work and the potential for self-assembly into...
A protein with a molecular mass of 42 kDa (P42) from Mycoplasma mobile, one of several mycoplasmas that exhibit gliding motility, was shown to be a novel NTPase (nucleoside triphosphatase). Although...
Cytoskeletal “jellyfish” structure of Mycoplasma mobile
Nakane, Daisuke, Miyata, Makoto
Mycoplasma mobile, a parasitic bacterium lacking a peptidoglycan layer, glides on solid surfaces in the direction of a membrane protrusion at a cell pole by a unique mechanism. Recently, we proposed...
Uenoyama, Atsuko, Seto, Shintaro, Nakane, Daisuke, Miyata, Makoto
Mycoplasma mobile glides on solid surfaces by use of a unique mechanism that involves two large proteins, Gli349 and Gli521. Here we isolated and analyzed two antibodies and three mutants that...
Nakane, Daisuke, Miyata, Makoto
Several mycoplasma species feature a membrane protrusion at a cell pole, and unknown mechanisms provide gliding motility in the direction of the pole defined by the protrusion. Mycoplasma...