K28, a unique double-stranded RNA killer virus of Saccharomyces cerevisiae (1990)
Schmitt, Manfred J., Tipper, Donald J.
The double-stranded RNA (dsRNA) viruses of Saccharomyces cerevisiae consist of 4.5-kilobase-pair (kb) L species and 1.7- to 2.1-kb M species, both found in cytoplasmic viruslike particles (VLPs). The...
Eiden-Plach, Antje, Zagorc, Tatjana, Heintel, Tanja, Carius, Yvonne, Breinig, Frank, Schmitt, Manfred J.
Besides its importance as model organism in eukaryotic cell biology, yeast species have also developed into an attractive host for the expression, processing, and secretion of recombinant proteins....
Dual targeting of yeast catalase A to peroxisomes and mitochondria.
Petrova, Ventsislava Y, Drescher, Diane, Kujumdzieva, Anna V, Schmitt, Manfred J
Yeast catalase A (Cta1p) contains two peroxisomal targeting signals (SSNSKF) localized at its C-terminus and within the N-terminal third of the protein, which both can target foreign proteins to...
Breinig, Frank, Sendzik, Tanja, Eisfeld, Katrin, Schmitt, Manfred J.
Toxin-secreting “killer” yeasts were initially identified >40 years ago in Saccharomyces cerevisiae strains infected with a double-stranded RNA “killer” virus. Despite extensive research...
Eiden-Plach, Antje, Zagorc, Tatjana, Heintel, Tanja, Carius, Yvonne, Breinig, Frank, Schmitt, Manfred J.
Besides its importance as model organism in eukaryotic cell biology, yeast species have also developed into an attractive host for the expression, processing, and secretion of recombinant proteins....
Dual targeting of yeast catalase A to peroxisomes and mitochondria.
Petrova, Ventsislava Y, Drescher, Diane, Kujumdzieva, Anna V, Schmitt, Manfred J
Yeast catalase A (Cta1p) contains two peroxisomal targeting signals (SSNSKF) localized at its C-terminus and within the N-terminal third of the protein, which both can target foreign proteins to...
Breinig, Frank, Sendzik, Tanja, Eisfeld, Katrin, Schmitt, Manfred J.
Toxin-secreting “killer” yeasts were initially identified >40 years ago in Saccharomyces cerevisiae strains infected with a double-stranded RNA “killer” virus. Despite extensive research...
Breinig, Frank, Diehl, Björn, Rau, Sabrina, Zimmer, Christian, Schwab, Helmut, Schmitt, Manfred J.
Yeast cell surface display is a powerful tool for expression and immobilization of biocatalytically active proteins on a unicellular eukaryote. Here bacterial carboxylesterase EstA from Burkholderia...
Powilleit, Frank, Breinig, Tanja, Schmitt, Manfred J.
A novel expression system based on engineered variants of the yeast (Saccharomyces cerevisiae) dsRNA virus L-A was developed allowing the in vivo assembly of chimeric virus-like particles (VLPs) as a...
Heiligenstein, Susanne, Eisfeld, Katrin, Sendzik, Tanja, Jimenéz-Becker, Natalia, Breinig, Frank, Schmitt, Manfred J
K28 is a viral A/B toxin that traverses eukaryotic cells by endocytosis and retrograde transport through the secretory pathway. Here we show that toxin retrotranslocation from the endoplasmic...
Viral killer toxins induce caspase-mediated apoptosis in yeast
Reiter, Jochen, Herker, Eva, Madeo, Frank, Schmitt, Manfred J.
In yeast, apoptotic cell death can be triggered by various factors such as H2O2, cell aging, or acetic acid. Yeast caspase (Yca1p) and cellular reactive oxygen species (ROS) are key regulators of...