Manolis Kellis

The consensus coding sequence (CCDS) project: Identifying a common protein-coding gene set for the human and mouse genomes (2009)

Pruitt, Kim D., Harrow, Jennifer, Harte, Rachel A., Wallin, Craig, Diekhans, Mark, Maglott, Donna R., ...

Effective use of the human and mouse genomes requires reliable identification of genes and their products. Although multiple public resources provide annotation, different methods are used that can...

HST.480: BIOINFORMATICS AND PROTEOMICS: AN ENGINEERING-BASED PROBLEM SOLVING APPROACH (2008)

Marco Ramoni, Manolis Kellis

Interdisciplinary and hands-on approach the topics of bioinformatics and proteomics. Lectures and labs cover sequence analysis, microarray expression analysis, Bayesian methods, control theory,...

Special review for the Encyclopedia of Genomics, Proteomics, and Bioinformatics. (2008)

Manolis Kellis, John Wiley, (in Press

Three years after the initial sequencing of the human genome, the actual number of functional human genes remains uncertain. Several expression-based analyses still argue for a hundred thousand...

A Classification Approach to Comparative Gene Finding in Mammals (2008)

Michael Lin, Manolis Kellis

Evolutionary conservation is a powerful signal that can be used to identify protein-coding genes within related genomes. Promising early approaches ([1], [3], [4], [5]) considered conservation...

The evolutionary dynamics of the Saccharomyces cerevisiae protein interaction network after duplication (2008)

Presser, Aviva, Elowitz, Michael B., Kellis, Manolis, Kishony, Roy

Gene duplication is an important mechanism in the evolution of protein interaction networks. Duplications are followed by the gain and loss of interactions, rewiring the network at some unknown rate....

Genome analysis of the platypus reveals unique signatures of evolution (2008)

Warren, Wesley C., Hillier, LaDeana W., Marshall Graves, Jennifer A., Birney, Ewan, Ponting, Chris P., Grützner, Frank, ...

We present a draft genome sequence of the platypus, Ornithorhynchus anatinus. This monotreme exhibits a fascinating combination of reptilian and mammalian characters. For example, platypuses have a...

A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands (2008)

Stark, Alexander, Bushati, Natascha, Jan, Calvin H., Kheradpour, Pouya, Hodges, Emily, Brennecke, Julius, ...

MicroRNAs (miRNAs) are ∼22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression....

Conservation of small RNA pathways in platypus (2008)

Murchison, Elizabeth P., Kheradpour, Pouya, Sachidanandam, Ravi, Smith, Carly, Hodges, Emily, Xuan, Zhenyu, ...

Small RNA pathways play evolutionarily conserved roles in gene regulation and defense from parasitic nucleic acids. The character and expression patterns of small RNAs show conservation throughout...

Network motif discovery using subgraph enumeration and symmetry-breaking (2007)

Joshua A. Grochow, Manolis Kellis

Abstract. The study of biological networks and network motifs can yield significant new insights into systems biology. Previous methods of discovering network motifs – network-centric subgraph...

Revisiting the protein-coding gene catalog of Drosophila melanogaster using 12 fly genomes (2007)

Lin, Michael F., Carlson, Joseph W., Crosby, Madeline A., Matthews, Beverley B., Yu, Charles, Park, Soo, ...

The availability of sequenced genomes from 12 Drosophila species has enabled the use of comparative genomics for the systematic discovery of functional elements conserved within this genus. We have...

Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs (2007)

Ruby, J. Graham, Stark, Alexander, Johnston, Wendy K., Kellis, Manolis, Bartel, David P., Lai, Eric C.

MicroRNA (miRNA) genes give rise to small regulatory RNAs in a wide variety of organisms. We used computational methods to predict miRNAs conserved among Drosophila species and large-scale sequencing...

Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes (2007)

Stark, Alexander, Kheradpour, Pouya, Parts, Leopold, Brennecke, Julius, Hodges, Emily, Hannon, Gregory J., ...

MicroRNAs (miRNAs) are short regulatory RNAs that inhibit target genes by complementary binding in 3′ untranslated regions (3′ UTRs). They are one of the most abundant classes of regulators,...

Reliable prediction of regulator targets using 12 Drosophila genomes (2007)

Kheradpour, Pouya, Stark, Alexander, Roy, Sushmita, Kellis, Manolis

Gene expression is regulated pre- and post-transcriptionally via cis-regulatory DNA and RNA motifs. Identification of individual functional instances of such motifs in genome sequences is a major...

Accurate gene-tree reconstruction by learning gene- and species-specific substitution rates across multiple complete genomes (2007)

Rasmussen, Matthew D., Kellis, Manolis

Comparative genomics provides a general methodology for discovering functional DNA elements and understanding their evolution. The availability of many related genomes enables more powerful analyses,...

Whole-genome ChIP-chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo (2007)

Zeitlinger, Julia, Zinzen, Robert P., Stark, Alexander, Kellis, Manolis, Zhang, Hailan, Young, Richard A., ...

Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined...

Networks (2006)

Joshua A. Grochow, Manolis Kellis, Joshua A. Grochow

The study of protein interactions from the networks point of view has yielded new insights into systems biology [Bar03, MA03, RSM + 02, WS98]. In particular, “network motifs ” become apparent as...

Large-scale discovery and validation of functional elements in the human genome (2005)

Bernstein, Bradley E, Kellis, Manolis

A report on the genomics workshop 'Identification of Functional Elements in Mammalian Genomes', Cold Spring Harbor, New York, 11-13 November 2004.

The changing face of genomics (2004)

Kellis, Manolis

A report on the 5th annual Advances in Genome Biology and Technology (AGBT) and Automation in DNA Mapping and Sequencing (AMS) meeting, Marco Island, USA, 4-7 February 2004.

Introduction to Bioinformatics (2004)

Neil C. Jones, Pavel A. Pevzner, Manolis Kellis

Comparative genomics reveals unusually long motifs in mammalian genomes Vol. 22 no. 14 2006, pages e236–e242 doi:10.1093/bioinformatics/btl265

ES: Methods in comparative genomics: genome correspondence, gene identification and regulatory motif discovery (2004)

Manolis Kellis, Nick Patterson, Bruce Birren, Bonnie Berger, Eric S. L

In Kellis et al. (2003), we reported the genome sequences of S. paradoxus, S. mikatae and S. bayanus and compared these three yeast species to their close relative, S. cerevisiae. Genome-wide...

Position specific variation in the rate of evolution in transcription factor binding sites (2003)

Moses, Alan M., Chiang, Derek Y., Kellis, Manolis, Lander, Eric S., Eisen, Michael B.

Background: The binding sites of sequence specific transcription factors are an important and relatively well-understood class of functional non-coding DNAs. Although a wide variety of experimental...

Position specific variation in the rate of evolution in transcription factor binding sites (2003)

Moses, Alan M, Chiang, Derek Y, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

Abstract Background The binding sites of sequence specific transcription factors are an important and relatively well-understood class of functional non-coding DNAs. Although a wide variety of...

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts (2003)

Chiang, Derek Y, Moses, Alan M, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

Abstract Background Transcriptional regulation in eukaryotes often involves multiple transcription factors binding to the same transcription control region, and to understand the regulatory content...

BMC Evolutionary Biology BioMed Central (2003)

Alan M Moses, Derek Y Chiang, Manolis Kellis, Eric S L, Michael B Eisen, Eric S Lander, ...

Research article Position specific variation in the rate of evolution in transcription factor binding sites

2003 Chiang et Volume al. 4, Issue 7, Article R43 Open Access (2003)

Derek Y Chiang, Alan M Moses, Manolis Kellis, Eric S L, Michael B Eisen

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts

BMC Evolutionary Biology BioMed Central (2003)

Alan M Moses, Derek Y Chiang, Manolis Kellis, Eric S L, Michael B Eisen, Eric S Lander, ...

Research article Position specific variation in the rate of evolution in transcription factor binding sites

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts

Chiang, Derek Y, Moses, Alan M, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

The determination of conserved sequences has identified individual transcription factor binding sites. The incorporation of both joint conservation and spacing constraints of sequence pairs predicts...

The changing face of genomics

Kellis, Manolis

A report on the 5th annual Advances in Genome Biology and Technology (AGBT) and Automation in DNA Mapping and Sequencing (AMS) meeting, Marco Island, USA, 4-7 February 2004.

Large-scale discovery and validation of functional elements in the human genome

Bernstein, Bradley E, Kellis, Manolis

A report on the genomics workshop 'Identification of Functional Elements in Mammalian Genomes', Cold Spring Harbor, New York, 11-13 November 2004.

Phylogenetically and spatially conserved word pairs associated with gene-expression changes in yeasts

Chiang, Derek Y, Moses, Alan M, Kellis, Manolis, Lander, Eric S, Eisen, Michael B

The determination of conserved sequences has identified individual transcription factor binding sites. The incorporation of both joint conservation and spacing constraints of sequence pairs predicts...

The changing face of genomics

Kellis, Manolis

A report on the 5th annual Advances in Genome Biology and Technology (AGBT) and Automation in DNA Mapping and Sequencing (AMS) meeting, Marco Island, USA, 4-7 February 2004.

Large-scale discovery and validation of functional elements in the human genome

Bernstein, Bradley E, Kellis, Manolis

A report on the genomics workshop 'Identification of Functional Elements in Mammalian Genomes', Cold Spring Harbor, New York, 11-13 November 2004.

Whole-genome ChIP–chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo

Zeitlinger, Julia, Zinzen, Robert P., Stark, Alexander, Kellis, Manolis, Zhang, Hailan, Young, Richard A., ...

Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined...

Systematic discovery of regulatory motifs in conserved regions of the human genome, including thousands of CTCF insulator sites

Xie, Xiaohui, Mikkelsen, Tarjei S., Gnirke, Andreas, Lindblad-Toh, Kerstin, Kellis, Manolis, Lander, Eric S.

Conserved noncoding elements (CNEs) constitute the majority of sequences under purifying selection in the human genome, yet their function remains largely unknown. Experimental evidence suggests that...

Performance and Scalability of Discriminative Metrics for Comparative Gene Identification in 12 Drosophila Genomes

Lin, Michael F., Deoras, Ameya N., Rasmussen, Matthew D., Kellis, Manolis

Comparative genomics of multiple related species is a powerful methodology for the discovery of functional genomic elements, and its power should increase with the number of species compared. Here,...

Distinguishing protein-coding and noncoding genes in the human genome

Clamp, Michele, Fry, Ben, Kamal, Mike, Xie, Xiaohui, Cuff, James, Lin, Michael F., ...

Although the Human Genome Project was completed 4 years ago, the catalog of human protein-coding genes remains a matter of controversy. Current catalogs list a total of ≈24,500 putative...

Revisiting the protein-coding gene catalog of Drosophila melanogaster using 12 fly genomes

Lin, Michael F., Carlson, Joseph W., Crosby, Madeline A., Matthews, Beverley B., Yu, Charles, Park, Soo, ...

The availability of sequenced genomes from 12 Drosophila species has enabled the use of comparative genomics for the systematic discovery of functional elements conserved within this genus. We have...

Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs

Ruby, J. Graham, Stark, Alexander, Johnston, Wendy K., Kellis, Manolis, Bartel, David P., Lai, Eric C.

MicroRNA (miRNA) genes give rise to small regulatory RNAs in a wide variety of organisms. We used computational methods to predict miRNAs conserved among Drosophila species and large-scale sequencing...

Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes

Stark, Alexander, Kheradpour, Pouya, Parts, Leopold, Brennecke, Julius, Hodges, Emily, Hannon, Gregory J., ...

MicroRNAs (miRNAs) are short regulatory RNAs that inhibit target genes by complementary binding in 3′ untranslated regions (3′ UTRs). They are one of the most abundant classes of regulators,...

Reliable prediction of regulator targets using 12 Drosophila genomes

Kheradpour, Pouya, Stark, Alexander, Roy, Sushmita, Kellis, Manolis

Gene expression is regulated pre- and post-transcriptionally via cis-regulatory DNA and RNA motifs. Identification of individual functional instances of such motifs in genome sequences is a major...

Accurate gene-tree reconstruction by learning gene- and species-specific substitution rates across multiple complete genomes

Rasmussen, Matthew D., Kellis, Manolis

Comparative genomics provides a general methodology for discovering functional DNA elements and understanding their evolution. The availability of many related genomes enables more powerful analyses,...

A single Hox locus in Drosophila produces functional microRNAs from opposite DNA strands

Stark, Alexander, Bushati, Natascha, Jan, Calvin H., Kheradpour, Pouya, Hodges, Emily, Brennecke, Julius, ...

MicroRNAs (miRNAs) are ∼22-nucleotide RNAs that are processed from characteristic precursor hairpins and pair to sites in messages of protein-coding genes to direct post-transcriptional repression....

The evolutionary dynamics of the Saccharomyces cerevisiae protein interaction network after duplication

Presser, Aviva, Elowitz, Michael B., Kellis, Manolis, Kishony, Roy

Gene duplication is an important mechanism in the evolution of protein interaction networks. Duplications are followed by the gain and loss of interactions, rewiring the network at some unknown rate....

Conservation of small RNA pathways in platypus

Murchison, Elizabeth P., Kheradpour, Pouya, Sachidanandam, Ravi, Smith, Carly, Hodges, Emily, Xuan, Zhenyu, ...

Small RNA pathways play evolutionarily conserved roles in gene regulation and defense from parasitic nucleic acids. The character and expression patterns of small RNAs show conservation throughout...