The Search for a Biological Energy Transducer (1989)
Muscle can be thought of as an engine—an assembly of devices capable of performing useful work at the expense of degrading fuel. Among the devices the most distinctive is the transducer, wherein...
An Attempt to Detect Phosphorylated Myosin by ADP-ATP Exchange (1971)
IKEZAWA, Hiroh, IKEZAWA, Kimiko, TONOMURA, Yuji, MORALES, Manuel F.
1. For the purpose of detection of the intermediate E—OP in the reaction of myosin-ATPase [EC 3.6.1.3], the possibility of back-incorporation of 14C from labelled ADP into ATP was examined. The...
Includes bibliographies.
On the tryptophan residue of smooth muscle myosin that responds to binding of nucleotide
Onishi, Hirofumi, Konishi, Kaoru, Fujiwara, Keigi, Hayakawa, Kazuyoshi, Tanokura, Masaru, Martinez, Hugo M., ...
Initially, we asked which (of 10) smooth muscle myosin head residues responds to MgADP or MgATP binding with enhanced fluorescence emission (Trp-441 and Trp-512 were leading candidates)? To decide,...
Onishi, Hirofumi, Kojima, Shin-ichiro, Katoh, Kazuo, Fujiwara, Keigi, Martinez, Hugo M., Morales, Manuel F.
For analyzing the mechanism of energy transduction in the “motor” protein, myosin, it is opportune both to model the structural change in the hydrolytic transition, ATP (myosin-bound) + H2O →...
Consequences of placing an intramolecular crosslink in myosin S1
Konno, Kunihiko, Ue, Kathleen, Khoroshev, Mikhail, Martinez, Hugo, Ray, Bruce, Morales, Manuel F.
This paper describes the placement of a crosslinking agent (dibromobimane) between two thiols (Cys-522 and Cys-707) of a fragment, “S1,” of the motor protein, myosin. It turns out that fastening...
Onishi, Hirofumi, Ohki, Takashi, Mochizuki, Naoki, Morales, Manuel F.
On the basis of the crystallographic snapshots of Rayment and his collaborators [Fisher, A. J., Smith, C. A., Thoden, J. B., Smith, R., Sutoh, K., Holden, H. M., & Rayment, I. (1995) Biochemistry 34,...
Creatine and the Control of Myosin Synthesis in Differentiating Skeletal Muscle
Ingwall, Joanne S., Morales, Manuel F., Stockdale, Frank E.
These experiments provide evidence that creatine, an end product of contraction unique to muscle, is involved in the control of muscle-protein synthesis. Skeletal muscle cells formed both in vitro...
Individual States in the Cycle of Muscle Contraction
Remedios, Cristobal G. Dos, Yount, Ralph G., Morales, Manuel F.
By using appropriate analogs of ATP, isometrically-held glycerol-extracted psoas fibers from rabbits are forced successively into states corresponding to molecular species in the contractile cycle....
Tonomura, Yuji, Morales, Manuel F.
The ATPase (EC 3.6.1.3) of sarcoplasmic reticulum vesicles was reacted to various extents with thiol-directed spin labels. By suspension of the preparation in appropriate solutions, the enzyme could...
Toward understanding actin activation of myosin ATPase: The role of myosin surface loops
Onishi, Hirofumi, Mikhailenko, Sergey V., Morales, Manuel F.
To understand the complicated interplay when a traveling myosin head reaches interaction distance with two actins in a filament we looked to three myosin loops that early on exert their influences...
On the tryptophan residue of smooth muscle myosin that responds to binding of nucleotide
Onishi, Hirofumi, Konishi, Kaoru, Fujiwara, Keigi, Hayakawa, Kazuyoshi, Tanokura, Masaru, Martinez, Hugo M., ...
Initially, we asked which (of 10) smooth muscle myosin head residues responds to MgADP or MgATP binding with enhanced fluorescence emission (Trp-441 and Trp-512 were leading candidates)? To decide,...
Onishi, Hirofumi, Kojima, Shin-ichiro, Katoh, Kazuo, Fujiwara, Keigi, Martinez, Hugo M., Morales, Manuel F.
For analyzing the mechanism of energy transduction in the “motor” protein, myosin, it is opportune both to model the structural change in the hydrolytic transition, ATP (myosin-bound) + H2O →...
Consequences of placing an intramolecular crosslink in myosin S1
Konno, Kunihiko, Ue, Kathleen, Khoroshev, Mikhail, Martinez, Hugo, Ray, Bruce, Morales, Manuel F.
This paper describes the placement of a crosslinking agent (dibromobimane) between two thiols (Cys-522 and Cys-707) of a fragment, “S1,” of the motor protein, myosin. It turns out that fastening...
Onishi, Hirofumi, Ohki, Takashi, Mochizuki, Naoki, Morales, Manuel F.
On the basis of the crystallographic snapshots of Rayment and his collaborators [Fisher, A. J., Smith, C. A., Thoden, J. B., Smith, R., Sutoh, K., Holden, H. M., & Rayment, I. (1995) Biochemistry 34,...
Creatine and the Control of Myosin Synthesis in Differentiating Skeletal Muscle
Ingwall, Joanne S., Morales, Manuel F., Stockdale, Frank E.
These experiments provide evidence that creatine, an end product of contraction unique to muscle, is involved in the control of muscle-protein synthesis. Skeletal muscle cells formed both in vitro...
Individual States in the Cycle of Muscle Contraction
Remedios, Cristobal G. Dos, Yount, Ralph G., Morales, Manuel F.
By using appropriate analogs of ATP, isometrically-held glycerol-extracted psoas fibers from rabbits are forced successively into states corresponding to molecular species in the contractile cycle....
Tonomura, Yuji, Morales, Manuel F.
The ATPase (EC 3.6.1.3) of sarcoplasmic reticulum vesicles was reacted to various extents with thiol-directed spin labels. By suspension of the preparation in appropriate solutions, the enzyme could...
Toward understanding actin activation of myosin ATPase: The role of myosin surface loops
Onishi, Hirofumi, Mikhailenko, Sergey V., Morales, Manuel F.
To understand the complicated interplay when a traveling myosin head reaches interaction distance with two actins in a filament we looked to three myosin loops that early on exert their influences...
A closer look at energy transduction in muscle
Onishi, Hirofumi, Morales, Manuel F.
Muscular force is the sum of unitary force interactions generated as filaments of myosins move forcibly along parallel filaments of actins, understanding that the free energy required comes from...
SOME THERMOANALYTIC STUDIES OF ORGAN AND WHOLE ANIMAL RESPIRATION
Data on the respiratory rates of frog liver, kidney, and striated muscle were obtained at various temperatures by the Warburg method. Fundamental differences in the curves of QOO2 vs. T exist among...
A PHYSICAL THEORY OF ACID ANION DISPLACEMENT AND RECOVERY FOLLOWING EXERCISE
Morales, Manuel F., Shock, Nathan W.
A simple theoretical model has been presented whose behavior duplicates the variation in bicarbonate ion concentration in the blood following exercise. Methods for the evaluation of the constants of...
SPECIFICITY OF CREATINE IN THE CONTROL OF MUSCLE PROTEIN SYNTHESIS
Ingwall, Joanne S., Weiner, Cynthia D., Morales, Manuel F., Davis, Elaine, Stockdale, Frank E.
This study provides additional evidence that creatine, an end product of contraction unique to muscle, is involved in the control of muscle protein synthesis. Creatine is shown to stimulate...