Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly (2006)
Nolwenn Jouvenet, Cameron Bess, Marc C. Johnson, Cesar A. Virgen, Sanford M. Simon, ...
Recently proposed models that have gained wide acceptance posit that HIV-1 virion morphogenesis is initiated by targeting the major structural protein (Gag) to late endosomal membranes. Thereafter,...
Typescript (photocopy).
Johnson, Marc C., Simon, Benjamin E., Kim, Carol H., Leong, Jo-Ann C.
Snakehead rhabdovirus (SHRV) affects warm water fish in Southeast Asia and belongs to the genus Novirhabdovirus by virtue of its nonvirion gene (NV). Because SHRV grows best at temperatures between...
Kim, Carol H., Johnson, Marc C., Drennan, John D., Simon, Benjamin E., Thomann, Estela, Leong, Jo-Ann C.
Protective immunity by vaccination with plasmid DNA encoding a viral glycoprotein (G) has long been assumed to result from the induction of a specific immune response. We report here that the initial...
PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells
Johnson, Marc C., Scobie, Heather M., Vogt, Volker M.
While baculovirus expression of Gag proteins from numerous retroviruses has led reliably to production of virus-like particles (VLPs), we observed that expression of Rous sarcoma virus Gag failed to...
Nucleic Acid-Independent Retrovirus Assembly Can Be Driven by Dimerization
Johnson, Marc C., Scobie, Heather M., Ma, Yu May, Vogt*, Volker M.
The Gag protein of retroviruses alone can polymerize into regular virus-like particles (VLPs) both in vitro and in vivo. In most circumstances the capsid (CA) and nucleocapsid (NC) domains of Gag as...
Alonso, Marta, Kim, Carol H., Johnson, Marc C., Pressley, Meagan, Leong, Jo-Ann
Snakehead rhabdovirus (SHRV) affects warm-water fish in Southeast Asia and belongs to the genus Novirhabdovirus by virtue of its “nonvirion” (NV) gene. To examine the function of the NV gene, we...
Johnson, Marc C., Spidel, Jared L., Ako-Adjei, Danso, Wills, John W., Vogt, Volker M.
Retroviral late domains (L domains) are short amino acid sequences in the Gag protein that facilitate the process of budding. L domains act by recruiting the ESCRT complexes, which normally function...
Ako-Adjei, Danso, Johnson, Marc C., Vogt, Volker M.
The retroviral structural protein, Gag, is capable of independently assembling into virus-like particles (VLPs) in living cells and in vitro. Immature VLPs of human immunodeficiency virus type 1...
Visualization of retrovirus budding with correlated light and electron microscopy
Larson, Daniel R., Johnson, Marc C., Webb, Watt W., Vogt, Volker M.
We have used correlated scanning EM (SEM) and multiphoton fluorescence microscopy to visualize budding of virus-like particles (VLPs) of Rous sarcoma virus (RSV) and HIV type 1 (HIV-1). When the Gag...
Johnson, Marc C., Simon, Benjamin E., Kim, Carol H., Leong, Jo-Ann C.
Snakehead rhabdovirus (SHRV) affects warm water fish in Southeast Asia and belongs to the genus Novirhabdovirus by virtue of its nonvirion gene (NV). Because SHRV grows best at temperatures between...
Kim, Carol H., Johnson, Marc C., Drennan, John D., Simon, Benjamin E., Thomann, Estela, Leong, Jo-Ann C.
Protective immunity by vaccination with plasmid DNA encoding a viral glycoprotein (G) has long been assumed to result from the induction of a specific immune response. We report here that the initial...
PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells
Johnson, Marc C., Scobie, Heather M., Vogt, Volker M.
While baculovirus expression of Gag proteins from numerous retroviruses has led reliably to production of virus-like particles (VLPs), we observed that expression of Rous sarcoma virus Gag failed to...
Nucleic Acid-Independent Retrovirus Assembly Can Be Driven by Dimerization
Johnson, Marc C., Scobie, Heather M., Ma, Yu May, Vogt*, Volker M.
The Gag protein of retroviruses alone can polymerize into regular virus-like particles (VLPs) both in vitro and in vivo. In most circumstances the capsid (CA) and nucleocapsid (NC) domains of Gag as...
Alonso, Marta, Kim, Carol H., Johnson, Marc C., Pressley, Meagan, Leong, Jo-Ann
Snakehead rhabdovirus (SHRV) affects warm-water fish in Southeast Asia and belongs to the genus Novirhabdovirus by virtue of its “nonvirion” (NV) gene. To examine the function of the NV gene, we...
Johnson, Marc C., Spidel, Jared L., Ako-Adjei, Danso, Wills, John W., Vogt, Volker M.
Retroviral late domains (L domains) are short amino acid sequences in the Gag protein that facilitate the process of budding. L domains act by recruiting the ESCRT complexes, which normally function...
Ako-Adjei, Danso, Johnson, Marc C., Vogt, Volker M.
The retroviral structural protein, Gag, is capable of independently assembling into virus-like particles (VLPs) in living cells and in vitro. Immature VLPs of human immunodeficiency virus type 1...
Visualization of retrovirus budding with correlated light and electron microscopy
Larson, Daniel R., Johnson, Marc C., Webb, Watt W., Vogt, Volker M.
We have used correlated scanning EM (SEM) and multiphoton fluorescence microscopy to visualize budding of virus-like particles (VLPs) of Rous sarcoma virus (RSV) and HIV type 1 (HIV-1). When the Gag...
Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly
Jouvenet, Nolwenn, Neil, Stuart J. D, Bess, Cameron, Johnson, Marc C, Virgen, Cesar A, Simon, Sanford M, ...
Recently proposed models that have gained wide acceptance posit that HIV-1 virion morphogenesis is initiated by targeting the major structural protein (Gag) to late endosomal membranes. Thereafter,...
Bouamr, Fadila, Houck-Loomis, Brian R., De Los Santos, Martha, Casaday, Rebecca J., Johnson, Marc C., Goff, Stephen P.
The human immunodeficiency virus type 1 (HIV-1) Gag protein recruits Tsg101 to facilitate HIV-1 particle budding and release. In uninfected cells, the Hrs protein recruits the ESCRT-I complex to the...
Ubiquitin-dependent virus particle budding without viral protein ubiquitination
Zhadina, Maria, McClure, Myra O., Johnson, Marc C., Bieniasz, Paul D.
An essential step in the release of an extracellular enveloped virus particle is a budding event that ultimately separates virion and host cell membranes. For many enveloped viruses, membrane fission...
Keller, Paul W., Johnson, Marc C., Vogt, Volker M.
All orthoretroviruses encode a single structural protein, Gag, which is necessary and sufficient for the assembly and budding of enveloped virus-like particles from the cell. The Gag proteins of Rous...
Foreign Glycoproteins Can Be Actively Recruited to Virus Assembly Sites during Pseudotyping▿
Jorgenson, Rebecca L., Vogt, Volker M., Johnson, Marc C.
Retroviruses like human immunodeficiency virus type 1 (HIV-1), as well as many other enveloped viruses, can efficiently produce infectious virus in the absence of their own surface glycoprotein if a...