mwr Xer site-specific recombination is hypersensitive to DNA supercoiling (2009)
Trigueros, Sonia, Tran, Tung, Sorto, Nohemy, Newmark, Jason, Colloms, Sean D., Sherratt, David J., ...
The multiresistance plasmid pJHCMW1, first identified in a Klebsiella pneumoniae strain isolated from a neonate with meningitis, includes a Xer recombination site, mwr, with unique characteristics....
Functional characterization of Tn1331 gene cassettes (2008)
Ramirez, Maria S., Parenteau, T. Richard, Centron, Daniela, Tolmasky, Marcelo E.
Objectives The transposon Tn1331 possesses a region including three antibiotic resistance genes with the structure aac(6′)-Ib-attC-aadA1-attI1*-blaOXA-9-attC, which potentially includes four gene...
Chavideh, Ramona, Sholly, Steven, Panaite, Doina, Tolmasky, Marcelo E.
Substitutions at position F171 of 6′-N-acetyltransferase type Ib cause variable loss of aminoglycoside resistance, indicating that this residue plays an important role in the structure and/or...
Systematic Analysis of a Conserved Region of the Aminoglycoside 6′-N-Acetyltransferase Type Ib
Shmara, Ali, Weinsetel, Natalia, Dery, Ken J., Chavideh, Ramona, Tolmasky, Marcelo E.
Alanine-scanning mutagenesis was applied to the aminoglycoside 6′-N-acetyltransferase type Ib conserved motif B, and the effects of the substitutions were analyzed by measuring the MICs of...
Complete Nucleotide Sequence of Klebsiella pneumoniae Multiresistance Plasmid pJHCMW1
Sarno, Renee, McGillivary, Glen, Sherratt, David J., Actis, Luis A., Tolmasky, Marcelo E.
The multiresistance plasmid pJHCMW1, harbored by a clinical Klebsiella pneumoniae strain isolated from a neonate with meningitis, was sequenced. A circular sequence of 11,354 bp was generated, of...
Pham, Huong, Dery, Ken J., Sherratt, David J., Tolmasky, Marcelo E.
Xer-mediated dimer resolution at the mwr site of plasmid pJHCMW1 is osmoregulated in Escherichia coli. Whereas under low-salt conditions, the site-specific recombination reaction is efficient, under...
Dery, Ken J., Søballe, Britta, Witherspoon, Mavee S. L., Bui, Duyen, Koch, Robert, Sherratt, David J., ...
The multiresistance transposon Tn1331, which mediates resistance to several aminoglycosides and β-lactams, includes the aac(6′)-Ib, aadA1, blaOXA-9, and blaTEM-1 genes. The nucleotide sequence of...
Di Lorenzo, Manuela, Stork, Michiel, Tolmasky, Marcelo E., Actis, Luis A., Farrell, David, Welch, Timothy J., ...
The virulence plasmid pJM1 enables the fish pathogen Vibrio anguillarum, a gram-negative polarly flagellated comma-shaped rod bacterium, to cause a highly fatal hemorrhagic septicemic disease in...
Sarno, Renee, Ha, Hongphuc, Weinsetel, Natalia, Tolmasky, Marcelo E.
Amikacin has been very useful in the treatment of infections caused by multiresistant bacteria because it is refractory to the actions of most modifying enzymes. However, the spread of...
O'Rourke, Eyleen J., Pinto, A. Viviana, Petroni, E. Alejandro, Tolmasky, Marcelo E., Ielpi, Luis
Helicobacter pylori is a gram-negative bacterium that colonizes the human stomach, causes gastritis, and is associated with ulcers and gastric cancer. H. pylori is naturally competent for...
Transfer of Oligosaccharide to Protein from a Lipid Intermediate in Plants
Staneloni, Roberto J., Tolmasky, Marcelo E., Petriella, Claudio, Leloir, Luis F.
A lipid-bound oligosaccharide was isolated from pea (Pisum sativum) cotyledons incubated with [14C]mannose. The oligosaccharide moiety appeared to be identical with the one obtained from rat liver,...
Di Lorenzo, Manuela, Poppelaars, Sophie, Stork, Michiel, Nagasawa, Maho, Tolmasky, Marcelo E., Crosa, Jorge H.
Anguibactin, a siderophore produced by Vibrio anguillarum, is synthesized via a nonribosomal peptide synthetase (NRPS) mechanism. We have identified a gene from the V. anguillarum plasmid pJM1 that...
Whole-Genome Analyses of Speciation Events in Pathogenic Brucellae†
Chain, Patrick S. G., Comerci, Diego J., Tolmasky, Marcelo E., Larimer, Frank W., Malfatti, Stephanie A., Vergez, Lisa M., ...
Despite their high DNA identity and a proposal to group classical Brucella species as biovars of Brucella melitensis, the commonly recognized Brucella species can be distinguished by distinct...
Pourreza, Atousa, Witherspoon, Mavee, Fox, Jessica, Newmark, Jason, Bui, Duyen, Tolmasky, Marcelo E.
Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6′-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of...
Bui, Duyen, Ramiscal, Judianne, Trigueros, Sonia, Newmark, Jason S., Do, Albert, Sherratt, David J., ...
Xer-mediated dimer resolution at the mwr site of the multiresistance plasmid pJHCMW1 is osmoregulated in Escherichia coli containing either the Escherichia coli Xer recombination machinery or Xer...
Martín, Fernando A., Petroni, Alejandro, Faccone, Diego, Galas, Marcelo, Tolmasky, Marcelo E., ...
A ca. 150-kbp Vibrio cholerae O1 biotype El Tor plasmid includes blaCTX-M-2 and a variant of aac(6′)-Ib within InV117, an orf513-bearing class 1 integron. InV117 is linked to a tnp1696 module in...
Chavideh, Ramona, Sholly, Steven, Panaite, Doina, Tolmasky, Marcelo E.
Substitutions at position F171 of 6′-N-acetyltransferase type Ib cause variable loss of aminoglycoside resistance, indicating that this residue plays an important role in the structure and/or...
Systematic Analysis of a Conserved Region of the Aminoglycoside 6′-N-Acetyltransferase Type Ib
Shmara, Ali, Weinsetel, Natalia, Dery, Ken J., Chavideh, Ramona, Tolmasky, Marcelo E.
Alanine-scanning mutagenesis was applied to the aminoglycoside 6′-N-acetyltransferase type Ib conserved motif B, and the effects of the substitutions were analyzed by measuring the MICs of...
Complete Nucleotide Sequence of Klebsiella pneumoniae Multiresistance Plasmid pJHCMW1
Sarno, Renee, McGillivary, Glen, Sherratt, David J., Actis, Luis A., Tolmasky, Marcelo E.
The multiresistance plasmid pJHCMW1, harbored by a clinical Klebsiella pneumoniae strain isolated from a neonate with meningitis, was sequenced. A circular sequence of 11,354 bp was generated, of...
Pham, Huong, Dery, Ken J., Sherratt, David J., Tolmasky, Marcelo E.
Xer-mediated dimer resolution at the mwr site of plasmid pJHCMW1 is osmoregulated in Escherichia coli. Whereas under low-salt conditions, the site-specific recombination reaction is efficient, under...
Dery, Ken J., Søballe, Britta, Witherspoon, Mavee S. L., Bui, Duyen, Koch, Robert, Sherratt, David J., ...
The multiresistance transposon Tn1331, which mediates resistance to several aminoglycosides and β-lactams, includes the aac(6′)-Ib, aadA1, blaOXA-9, and blaTEM-1 genes. The nucleotide sequence of...
Di Lorenzo, Manuela, Stork, Michiel, Tolmasky, Marcelo E., Actis, Luis A., Farrell, David, Welch, Timothy J., ...
The virulence plasmid pJM1 enables the fish pathogen Vibrio anguillarum, a gram-negative polarly flagellated comma-shaped rod bacterium, to cause a highly fatal hemorrhagic septicemic disease in...
Sarno, Renee, Ha, Hongphuc, Weinsetel, Natalia, Tolmasky, Marcelo E.
Amikacin has been very useful in the treatment of infections caused by multiresistant bacteria because it is refractory to the actions of most modifying enzymes. However, the spread of...
O'Rourke, Eyleen J., Pinto, A. Viviana, Petroni, E. Alejandro, Tolmasky, Marcelo E., Ielpi, Luis
Helicobacter pylori is a gram-negative bacterium that colonizes the human stomach, causes gastritis, and is associated with ulcers and gastric cancer. H. pylori is naturally competent for...
Transfer of Oligosaccharide to Protein from a Lipid Intermediate in Plants
Staneloni, Roberto J., Tolmasky, Marcelo E., Petriella, Claudio, Leloir, Luis F.
A lipid-bound oligosaccharide was isolated from pea (Pisum sativum) cotyledons incubated with [14C]mannose. The oligosaccharide moiety appeared to be identical with the one obtained from rat liver,...
Di Lorenzo, Manuela, Poppelaars, Sophie, Stork, Michiel, Nagasawa, Maho, Tolmasky, Marcelo E., Crosa, Jorge H.
Anguibactin, a siderophore produced by Vibrio anguillarum, is synthesized via a nonribosomal peptide synthetase (NRPS) mechanism. We have identified a gene from the V. anguillarum plasmid pJM1 that...
Pourreza, Atousa, Witherspoon, Mavee, Fox, Jessica, Newmark, Jason, Bui, Duyen, Tolmasky, Marcelo E.
Alanine scanning of motif A in the pJHCMW1-encoded aminoglycoside 6′-N-acetyltransferase type Ib identified amino acids important for the ability of the enzyme to confer wild-type levels of...
Whole-Genome Analyses of Speciation Events in Pathogenic Brucellae†
Chain, Patrick S. G., Comerci, Diego J., Tolmasky, Marcelo E., Larimer, Frank W., Malfatti, Stephanie A., Vergez, Lisa M., ...
Despite their high DNA identity and a proposal to group classical Brucella species as biovars of Brucella melitensis, the commonly recognized Brucella species can be distinguished by distinct...
Bui, Duyen, Ramiscal, Judianne, Trigueros, Sonia, Newmark, Jason S., Do, Albert, Sherratt, David J., ...
Xer-mediated dimer resolution at the mwr site of the multiresistance plasmid pJHCMW1 is osmoregulated in Escherichia coli containing either the Escherichia coli Xer recombination machinery or Xer...
Martín, Fernando A., Petroni, Alejandro, Faccone, Diego, Galas, Marcelo, Tolmasky, Marcelo E., ...
A ca. 150-kbp Vibrio cholerae O1 biotype El Tor plasmid includes blaCTX-M-2 and a variant of aac(6′)-Ib within InV117, an orf513-bearing class 1 integron. InV117 is linked to a tnp1696 module in...
Possoz, Christophe, Newmark, Jason, Sorto, Nohemy, Sherratt, David J., Tolmasky, Marcelo E.
Aminoglycosides bind to the 16S rRNA at the tRNA acceptor site (A site) and disturb protein synthesis by inducing codon misreading. We investigated Escherichia coli cell elongation and division, as...
External Guide Sequences Targeting the aac(6′)-Ib mRNA Induce Inhibition of Amikacin Resistance▿
Ha, Hongphuc, Sarno, Renee, Don, Michelle, Zorreguieta, Angeles, Tolmasky, Marcelo E.
The dissemination of AAC(6′)-I-type acetyltransferases have rendered amikacin and other aminoglycosides all but useless in some parts of the world. Antisense technologies could be an alternative to...
Quiroga, María Paula, Andres, Patricia, Petroni, Alejandro, Guerriero, Leonor, Vargas, Liliana Jordá, ...
Transferable quinolone resistance has not previously been reported in Argentina. Here we describe three complex class 1 integrons harboring the novel allele qnrB10 in a unique region downstream of...
mwr Xer site-specific recombination is hypersensitive to DNA supercoiling
Trigueros, Sonia, Tran, Tung, Sorto, Nohemy, Newmark, Jason, Colloms, Sean D., Sherratt, David J., ...
The multiresistance plasmid pJHCMW1, first identified in a Klebsiella pneumoniae strain isolated from a neonate with meningitis, includes a Xer recombination site, mwr, with unique characteristics....