Marisa S. Otegui

The Secretory System of Arabidopsis (2008)

Diane C. Bassham, Federica Brandizzi, Marisa S. Otegui, Anton A. Sanderfoot

Over the past few years, a vast amount of research has illuminated the workings of the secretory system of eukaryotic cells. The bulk of this work has been focused on the yeast Saccharomyces...

Three-Dimensional Analysis of Syncytial-Type Cell Plates during Endosperm Cellularization Visualized by High Resolution Electron Tomography W⃞

Otegui, Marisa S., Mastronarde, David N., Kang, Byung-Ho, Bednarek, Sebastian Y., Staehelin, L. Andrew

The three-dimensional architecture of syncytial-type cell plates in the endosperm of Arabidopsis has been analyzed at ∼6-nm resolution by means of dual-axis high-voltage electron tomography of...

Developing Seeds of Arabidopsis Store Different Minerals in Two Types of Vacuoles and in the Endoplasmic Reticulum

Otegui, Marisa S., Capp, Roberta, Staehelin, L. Andrew

Mineral-accumulating compartments in developing seeds of Arabidopsis were studied using high-pressure-frozen/freeze-substituted samples. Developing seeds store minerals in three locations: in the...

Three-Dimensional Analysis of Syncytial-Type Cell Plates during Endosperm Cellularization Visualized by High Resolution Electron Tomography W⃞

Otegui, Marisa S., Mastronarde, David N., Kang, Byung-Ho, Bednarek, Sebastian Y., Staehelin, L. Andrew

The three-dimensional architecture of syncytial-type cell plates in the endosperm of Arabidopsis has been analyzed at ∼6-nm resolution by means of dual-axis high-voltage electron tomography of...

Developing Seeds of Arabidopsis Store Different Minerals in Two Types of Vacuoles and in the Endoplasmic Reticulum

Otegui, Marisa S., Capp, Roberta, Staehelin, L. Andrew

Mineral-accumulating compartments in developing seeds of Arabidopsis were studied using high-pressure-frozen/freeze-substituted samples. Developing seeds store minerals in three locations: in the...

The Proteolytic Processing of Seed Storage Proteins in Arabidopsis Embryo Cells Starts in the Multivesicular Bodies[W]

Otegui, Marisa S., Herder, Rachel, Schulze, Jan, Jung, Rudolf, Staehelin, L. Andrew

We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor–Like Protein1 (VSR-1/ATELP1) receptor during...

A role for the RabA4b effector protein PI-4Kβ1 in polarized expansion of root hair cells in Arabidopsis thaliana

Preuss, Mary L., Schmitz, Aaron J., Thole, Julie M., Bonner, Heather K.S., Otegui, Marisa S., Nielsen, Erik

The RabA4b GTPase labels a novel, trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing Arabidopsis thaliana root hair cells. GTP bound RabA4b...

The Arabidopsis AAA ATPase SKD1 Is Involved in Multivesicular Endosome Function and Interacts with Its Positive Regulator LYST-INTERACTING PROTEIN5[W]

Haas, Thomas J., Sliwinski, Marek K., Martínez, Dana E., Preuss, Mary, Ebine, Kazuo, Ueda, Takashi, ...

In yeast and mammals, the AAA ATPase Vps4p/SKD1 (for Vacuolar protein sorting 4/SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) is required for the endosomal sorting of secretory and endocytic cargo. We...

INCREASED SIZE EXCLUSION LIMIT2 Encodes a Putative DEVH Box RNA Helicase Involved in Plasmodesmata Function during Arabidopsis Embryogenesis[W]

Kobayashi, Ken, Otegui, Marisa S., Krishnakumar, Sujatha, Mindrinos, Michael, Zambryski, Patricia

Here, we characterize the Arabidopsis thaliana embryo-defective mutant increased size exclusion limit2 (ise2). In contrast with wild-type embryos, ise2 mutants continue to traffic 10-kD fluorescent...

The Maize Floury1 Gene Encodes a Novel Endoplasmic Reticulum Protein Involved in Zein Protein Body Formation[W]

Holding, David R., Otegui, Marisa S., Li, Bailin, Meeley, Robert B., Dam, Thao, Hunter, Brenda G., ...

The maize (Zea mays) floury1 (fl1) mutant was first reported almost 100 years ago, but its molecular identity has remained unknown. We report the cloning of Fl1, which encodes a novel zein protein...

The Medicago truncatula DMI1 Protein Modulates Cytosolic Calcium Signaling1[W][OA]

Peiter, Edgar, Sun, Jongho, Heckmann, Anne B., Venkateshwaran, Muthusubramanian, Riely, Brendan K., Otegui, Marisa S., ...

In addition to establishing symbiotic relationships with arbuscular mycorrhizal fungi, legumes also enter into a nitrogen-fixing symbiosis with rhizobial bacteria that results in the formation of...

Subcellular Localization and Functional Domain Studies of DEFECTIVE KERNEL1 in Maize and Arabidopsis Suggest a Model for Aleurone Cell Fate Specification Involving CRINKLY4 and SUPERNUMERARY ALEURONE LAYER1[W]

Tian, Qing, Olsen, Lene, Sun, Beimeng, Lid, Stein Erik, Brown, Roy C., Lemmon, Betty E., ...

DEFECTIVE KERNEL1 (DEK1), which consists of a membrane-spanning region (DEK1-MEM) and a calpain-like Cys proteinase region (DEK1-CALP), is essential for aleurone cell formation at the surface of...

The Ubiquitin-Specific Protease Subfamily UBP3/UBP4 Is Essential for Pollen Development and Transmission in Arabidopsis1[W][OA]

Doelling, Jed H., Phillips, Allison R., Soyler-Ogretim, Gulsum, Wise, Jasen, Chandler, Jennifer, Callis, Judy, ...

Deubiquitinating enzymes are essential to the ubiquitin (Ub)/26S proteasome system where they release Ub monomers from the primary translation products of poly-Ub and Ub extension genes, recycle Ubs...

A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana[W][OA]

Ebine, Kazuo, Okatani, Yusuke, Uemura, Tomohiro, Goh, Tatsuaki, Shoda, Keiko, Niihama, Mitsuru, ...

The SNARE complex is a key regulator of vesicular traffic, executing membrane fusion between transport vesicles or organelles and target membranes. A functional SNARE complex consists of four...

A Role for the TOC Complex in Arabidopsis Root Gravitropism1[W][OA]

Stanga, John P., Boonsirichai, Kanokporn, Sedbrook, John C., Otegui, Marisa S., Masson, Patrick H.

Arabidopsis (Arabidopsis thaliana) roots perceive gravity and reorient their growth accordingly. Starch-dense amyloplasts within the columella cells of the root cap are important for gravitropism,...