7 Protein–Ligand Interaction Probed (2009)
Time-resolved Crystallography, Marius Schmidt, Hyotcherl Ihee, Reinhard Pahl, Vukica Srajer
Time-resolved (TR) crystallography is a unique method for determining the structures of intermediates in biomolecular reactions. The technique reached its mature stage with the development of the...
Protein kinetics: Structures of intermediates and reaction mechanism from time-resolved x-ray data
Schmidt, Marius, Pahl, Reinhard, Srajer, Vukica, Anderson, Spencer, Ren, Zhong, Ihee, Hyotcherl, ...
We determine the number of authentic reaction intermediates in the later stages of the photocycle of photoactive yellow protein at room temperature, their atomic structures, and a consistent set of...
Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds
Ihee, Hyotcherl, Rajagopal, Sudarshan, Šrajer, Vukica, Pahl, Reinhard, Anderson, Spencer, Schmidt, Marius, ...
Determining 3D intermediate structures during the biological action of proteins in real time under ambient conditions is essential for understanding how proteins function. Here we use time-resolved...
Schmidt, Marius, Nienhaus, Karin, Pahl, Reinhard, Krasselt, Angela, Anderson, Spencer, Parak, Fritz, ...
By using time-resolved x-ray crystallography at room temperature, structural relaxations and ligand migration were examined in myoglobin (Mb) mutant L29W from nanoseconds to seconds after...
Schmidt, Marius, Rajagopal, Sudarshan, Ren, Zhong, Moffat, Keith
Singular value decomposition (SVD) is a technique commonly used in the analysis of spectroscopic data that both acts as a noise filter and reduces the dimensionality of subsequent least-squares fits....
Protein kinetics: Structures of intermediates and reaction mechanism from time-resolved x-ray data
Schmidt, Marius, Pahl, Reinhard, Srajer, Vukica, Anderson, Spencer, Ren, Zhong, Ihee, Hyotcherl, ...
We determine the number of authentic reaction intermediates in the later stages of the photocycle of photoactive yellow protein at room temperature, their atomic structures, and a consistent set of...
Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds
Ihee, Hyotcherl, Rajagopal, Sudarshan, Šrajer, Vukica, Pahl, Reinhard, Anderson, Spencer, Schmidt, Marius, ...
Determining 3D intermediate structures during the biological action of proteins in real time under ambient conditions is essential for understanding how proteins function. Here we use time-resolved...
Schmidt, Marius, Nienhaus, Karin, Pahl, Reinhard, Krasselt, Angela, Anderson, Spencer, Parak, Fritz, ...
By using time-resolved x-ray crystallography at room temperature, structural relaxations and ligand migration were examined in myoglobin (Mb) mutant L29W from nanoseconds to seconds after...
Schmidt, Marius, Rajagopal, Sudarshan, Ren, Zhong, Moffat, Keith
Singular value decomposition (SVD) is a technique commonly used in the analysis of spectroscopic data that both acts as a noise filter and reduces the dimensionality of subsequent least-squares fits....
A Physical Picture of Protein Dynamics and Conformational Changes
Parak, Fritz G., Achterhold, Klaus, Croci, Simonetta, Schmidt, Marius
A physical model is reviewed which explains different aspects of protein dynamics consistently. At low temperatures, the molecules are frozen in conformational substates. Their average energy is...