Mark Hargreaves

Brief intense interval exercise activates AMPK and p38 MAPK signaling and increases the expression of PGC-1α in human skeletal muscle (2009)

Gibala, Martin J., McGee, Sean L, Garnham, Andrew P., Howlett, Kirsten F., Snow, Rodney J., Hargreaves, Mark

From a cell signaling perspective, short-duration intense muscular work is typically associated with resistance training and linked to pathways that stimulate growth. However, brief...

Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle (2007)

Howlett, Kirsten, Sakamoto, Kei, Garnham, Andrew, Cameron-Smith, David, Hargreaves, Mark

A single bout of aerobic exercise can enhance insulin action, but whether a similar effect occurs after resistance exercise is unknown. Hyperinsulinemic-euglycemic clamps were performed on eight male...

Reduced plasma free fatty acid availability during exercise: effect on gene expression (2007)

Tunstall, Rebecca J., McAinch, Andrew J., Hargreaves, Mark, Loon, Luc J. C., Cameron-Smith, David

Endurance exercise transiently increases the mRNA of key regulatory proteins involved in skeletal muscle metabolism. During prolonged exercise and subsequent recovery, circulating plasma fatty acid...

Reduced plasma free fatty acid availability during exercise: effect on gene expression (2007)

Tunstall, Rebecca J., McAinch, Andrew J., Hargreaves, Mark, Loon, Luc J. C., Cameron-Smith, David

Endurance exercise transiently increases the mRNA of key regulatory proteins involved in skeletal muscle metabolism. During prolonged exercise and subsequent recovery, circulating plasma fatty acid...

Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle (2007)

Howlett, Kirsten, Sakamoto, Kei, Garnham, Andrew, Cameron-Smith, David, Hargreaves, Mark

A single bout of aerobic exercise can enhance insulin action, but whether a similar effect occurs after resistance exercise is unknown. Hyperinsulinemic-euglycemic clamps were performed on eight male...

Insulin-stimulated insulin recetor substrate-2-associated phosphatidylinositol 3-kinase activity is enhanced in human skeletal muscle after exercise. (2006)

Howlett, Kirsten, Sakamoto, Kei, Yu, Haiyan, Goodyear, Laurie J., Hargreaves, Mark

Exercise increases skeletal muscle insulin action but the underlying mechanisms mediating this are equivocal. In mouse skeletal muscle, prior exercise enhances insulin-stimulated insulin receptor...

Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity (2006)

Kraniou, Giorgos N., Cameron-Smith, David, Hargreaves, Mark

To examine the influence of exercise intensity on the increases in vastus lateralis GLUT4 mRNA and protein after exercise, six untrained men exercised for 60 min at 39 ± 3% peak oxygen...

Exercise does not alter subcellular localization, but increases phosphorylation of insulin-signaling proteins in human skeletal muscle (2006)

Wilson, Chris, Hargreaves, Mark, Howlett, Kirsten

The subcellular localization of insulin signaling proteins is altered by various stimuli such as insulin, insulin-like growth factor I, and oxidative stress and is thought to be an important...

Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training. (2006)

Wadley, Glenn D., Konstantopoulos, Nicky, Macaulay, Lance, Howlett, Kirsten, Garnham, Andrew, Hargreaves, Mark, ...

The purpose of the present study was to determine in human skeletal muscle whether a single exercise bout and 7 days of consecutive endurance (cycling) training 1) increased insulin-stimulated Akt...

Insulin-stimulated insulin recetor substrate-2-associated phosphatidylinositol 3-kinase activity is enhanced in human skeletal muscle after exercise. (2006)

Howlett, Kirsten, Sakamoto, Kei, Yu, Haiyan, Goodyear, Laurie J., Hargreaves, Mark

Exercise increases skeletal muscle insulin action but the underlying mechanisms mediating this are equivocal. In mouse skeletal muscle, prior exercise enhances insulin-stimulated insulin receptor...

Exercise does not alter subcellular localization, but increases phosphorylation of insulin-signaling proteins in human skeletal muscle (2006)

Wilson, Chris, Hargreaves, Mark, Howlett, Kirsten

The subcellular localization of insulin signaling proteins is altered by various stimuli such as insulin, insulin-like growth factor I, and oxidative stress and is thought to be an important...

Acute exercise and GLUT4 expression in human skeletal muscle: influence of exercise intensity (2006)

Kraniou, Giorgos N., Cameron-Smith, David, Hargreaves, Mark

To examine the influence of exercise intensity on the increases in vastus lateralis GLUT4 mRNA and protein after exercise, six untrained men exercised for 60 min at 39 ± 3% peak oxygen...

Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training. (2006)

Wadley, Glenn D., Konstantopoulos, Nicky, Macaulay, Lance, Howlett, Kirsten, Garnham, Andrew, Hargreaves, Mark, ...

The purpose of the present study was to determine in human skeletal muscle whether a single exercise bout and 7 days of consecutive endurance (cycling) training 1) increased insulin-stimulated Akt...

Carbohydrate ingestion does not alter skeletal muscle AMPK signaling during exercise in humans (2006)

Lee-Young, Robert S., Palmer, Matthew J., Linden, Kelly C., LePlastrier, Kieran, Canny, Benedict J., Hargreaves, Mark, ...

There is evidence that increasing carbohydrate (CHO) availability during exercise by raising preexercise muscle glycogen levels attenuates the activation of AMPK{alpha}2 during exercise in humans....

Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle (2004)

Kraniou, Giorgos, Cameron-Smith, David, Hargreaves, Mark

Six untrained, male subjects (23 ± 1 years old, 84 ± 5 kg, VO2peak= 3.7 ± 0.8 l min–1) exercised for 60 min at 75 ± 1%VO2peak on 7 consecutive days.  Muscle...

Pyruvate dehydrogenase activation and kinase expression in human skeletal muscle during fasting (2004)

Spriet, Lawrence L., Tunstall, Rebecca J., Watt, Matthew J., Mehan, Kate A., Hargreaves, Mark, Cameron-Smith, David

Fasting forces adaptive changes in whole body and skeletal muscle metabolism that increase fat oxidation and decrease the oxidation of carbohydrate. We tested the hypothesis that 40 h of fasting...

Creatine supplementation increases glycogen storage but not GLUT-4 expression in human skeletal muscle (2004)

Murphy, Robyn, Oosterlaar, Audrey M., Cameron-Smith, David, Hargreaves, Mark, Wagenmakers, Anton J. M., ...

It has been speculated that creatine supplementation affects muscle glucose metabolism in humans by increasing muscle glycogen storage and up-regulating GLUT-4 protein expression. In the present...

Exercise and myocyte enhancer factor 2 regulation in human skeletal muscle (2004)

McGee, Sean, Hargreaves, Mark

Overexpression of GLUT4 in skeletal muscle enhances whole-body insulin action. Exercise increases GLUT4 gene and protein expression, and a binding site for the myocyte enhancer factor 2 (MEF-2) is...

Altering dietary nutrient intake that reduces glycogen content leads to phosphorylation of nuclear p38 MAP kinase in human skeletal muscle: association with IL-6 gene transcription during contraction (2004)

Chan, M.H. Stanley, McGee, Sean, Watt, Matthew J., Hargreaves, Mark, Febbraio, Mark A.

To determine the effect of glycogen availability and contraction on intracellular signaling and IL-6 gene transcription, eight males performed 60 min of exercise on two occasions: either with prior...

Exercise and myocyte enhancer factor 2 regulation in human skeletal muscle (2004)

McGee, Sean, Hargreaves, Mark

Overexpression of GLUT4 in skeletal muscle enhances whole-body insulin action. Exercise increases GLUT4 gene and protein expression, and a binding site for the myocyte enhancer factor 2 (MEF-2) is...

Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle (2004)

Kraniou, Giorgos, Cameron-Smith, David, Hargreaves, Mark

Six untrained, male subjects (23 ± 1 years old, 84 ± 5 kg, VO2peak= 3.7 ± 0.8 l min–1) exercised for 60 min at 75 ± 1%VO2peak on 7 consecutive days.  Muscle...

Creatine supplementation increases glycogen storage but not GLUT-4 expression in human skeletal muscle (2004)

Murphy, Robyn, Oosterlaar, Audrey M., Cameron-Smith, David, Hargreaves, Mark, Wagenmakers, Anton J. M., ...

It has been speculated that creatine supplementation affects muscle glucose metabolism in humans by increasing muscle glycogen storage and up-regulating GLUT-4 protein expression. In the present...

Pyruvate dehydrogenase activation and kinase expression in human skeletal muscle during fasting (2004)

Spriet, Lawrence L., Tunstall, Rebecca J., Watt, Matthew J., Mehan, Kate A., Hargreaves, Mark, Cameron-Smith, David

Fasting forces adaptive changes in whole body and skeletal muscle metabolism that increase fat oxidation and decrease the oxidation of carbohydrate. We tested the hypothesis that 40 h of fasting...

Altering dietary nutrient intake that reduces glycogen content leads to phosphorylation of nuclear p38 MAP kinase in human skeletal muscle: association with IL-6 gene transcription during contraction (2004)

Chan, M.H. Stanley, McGee, Sean, Watt, Matthew J., Hargreaves, Mark, Febbraio, Mark A.

To determine the effect of glycogen availability and contraction on intracellular signaling and IL-6 gene transcription, eight males performed 60 min of exercise on two occasions: either with prior...

Short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle (2003)

Cameron-Smith, David, Burke, Louise M., Angus, Damien J., Tunstall, Rebecca J ., Cox, Gregory R., Bonen, Arend, ...

Background: Dietary fatty acids may be important in regulating gene expression. However, little is known about the effect of changes in dietary fatty acids on gene regulation in human skeletal...

Effect of alcohol intake on muscle glycogen storage after prolonged exercise (2003)

Burke, Louise, Collier, Greg, Broad, Elizabeth, Davis, Peter, Martin, David, Sanigorski, Andrew, ...

We studied the effects of alcohol intake on postexercise muscle glycogen restoration with samples from vastus lateralis being collected immediately after glycogen-depleting cycling and after a set...

Effect of creatine ingestion on glucose tolerance and insulin sensitivity in men (2003)

Newman, Jackie, Hargreaves, Mark, Garnham, Andrew, Snow, Rodney

Purpose: This study investigated whether acute (5 d) and/or short-term (28 d) creatine (Cr) ingestion altered glucose tolerance or insulin action in healthy, untrained men (aged 26.9 ± 5.7 yr;...

Human skeletal muscle creatine transporter mRNA and protein expression in healthy, young males and females (2003)

Murphy, Robyn M., Tunstall, Rebecca J., Mehan, Kate A., Cameron-Smith, David, McKenna, Michael J., Spriet, Lawrence L., ...

The present study investigated whether there were any differences between males and females in respect to creatine transporter (CreaT) gene expression and/or total creatine (TCr) content in human...

Exercise increases nuclear AMPK α2 in human skeletal muscle (2003)

McGee, Sean, Howlett, Kirsten, Starkie, Rebecca L., Cameron-Smith, David, Kemp, Bruce E., Hargreaves, Mark

An acute bout of exercise increases skeletal muscle glucose uptake, improves glucose homeostasis and insulin sensitivity, and enhances muscle oxidative capacity. Recent studies have shown an...

Exercise increases nuclear AMPK α2 in human skeletal muscle (2003)

McGee, Sean, Howlett, Kirsten, Starkie, Rebecca L., Cameron-Smith, David, Kemp, Bruce E., Hargreaves, Mark

An acute bout of exercise increases skeletal muscle glucose uptake, improves glucose homeostasis and insulin sensitivity, and enhances muscle oxidative capacity. Recent studies have shown an...

Regulation of glucose kinetics during intense exercise in humans: effects of α- and β-adrenergic blockade (2003)

Howlett, Kirsten, Watt, Matthew J., Hargreaves, Mark, Febbraio, Mark A.

This study examined the effect of combined α- and β-adrenergic blockade on glucose kinetics during intense exercise. Six endurance-trained men exercised for 20 minutes at approximately 78%...

Effect of alcohol intake on muscle glycogen storage after prolonged exercise (2003)

Burke, Louise, Collier, Greg, Broad, Elizabeth, Davis, Peter, Martin, David, Sanigorski, Andrew, ...

We studied the effects of alcohol intake on postexercise muscle glycogen restoration with samples from vastus lateralis being collected immediately after glycogen-depleting cycling and after a set...

A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle (2003)

Cameron-Smith, David, Burke, Louise M., Angus, Damien J., Tunstall, Rebecca J ., Cox, Gregory R., Bonen, Arend, ...

Background: Dietary fatty acids may be important in regulating gene expression. However, little is known about the effect of changes in dietary fatty acids on gene regulation in human skeletal...

Effect of creatine ingestion on glucose tolerance and insulin sensitivity in men (2003)

Newman, Jackie, Hargreaves, Mark, Garnham, Andrew, Snow, Rodney

Purpose: This study investigated whether acute (5 d) and/or short-term (28 d) creatine (Cr) ingestion altered glucose tolerance or insulin action in healthy, untrained men (aged 26.9 ± 5.7 yr;...

Human skeletal muscle creatine transporter mRNA and protein expression in healthy, young males and females (2003)

Murphy, Robyn M., Tunstall, Rebecca J., Mehan, Kate A., Cameron-Smith, David, McKenna, Michael J., Spriet, Lawrence L., ...

The present study investigated whether there were any differences between males and females in respect to creatine transporter (CreaT) gene expression and/or total creatine (TCr) content in human...

Custom-built insulin resistance gene chip (2002)

Walder, Ken, Segal, David, Chehab, Sam, Augert, Guy, Cameron-Smith, David, Hargreaves, Mark, ...

Objectives/Aim—Microarray (gene chip) technology offers a powerful new tool for analyzing the expression of large numbers of genes in many experimental samples. The aim of this study was to...

Fasting activates the gene expression of UCP3 independent of genes necessary for lipid transport and oxidation in skeletal muscle (2002)

Tunstall, Rebecca, Mehan, Kate, Hargreaves, Mark, Spriet, Lawrence, Cameron-Smith, David

Fasting triggers a complex array of adaptive metabolic and hormonal responses including an augmentation in the capacity for mitochondrial fatty acid (FA) oxidation in skeletal muscle. This study...

Exercise, diet and skeletal muscle gene expression (2002)

Hargreaves, Mark, Cameron-Smith, David

Skeletal muscle, as a consequence of its mass and great capacity for altered metabolism, has a major impact on whole-body metabolic homeostasis and is capable of remarkable adaptation in response to...

Exercise training increases lipid metabolism gene expression in human skeletal muscle (2002)

Tunstall, Rebecca J., Mehan, Kate A., Wadley, Glenn D., Collier, Greg, Bonen, Arend, Hargreaves, Mark, ...

The effects of a single bout of exercise and exercise training on the expression of genes necessary for the transport and beta -oxidation of fatty acids (FA), together with the gene expression of...

Fasting activates the gene expression of UCP3 independent of genes necessary for lipid transport and oxidation in skeletal muscle (2002)

Tunstall, Rebecca, Mehan, Kate, Hargreaves, Mark, Spriet, Lawrence, Cameron-Smith, David

Fasting triggers a complex array of adaptive metabolic and hormonal responses including an augmentation in the capacity for mitochondrial fatty acid (FA) oxidation in skeletal muscle. This study...

A custom-built insulin resistance gene chip (2002)

Walder, Ken, Segal, David, Chehab, Sam, Augert, Guy, Cameron-Smith, David, Hargreaves, Mark, ...

Objectives/Aim—Microarray (gene chip) technology offers a powerful new tool for analyzing the expression of large numbers of genes in many experimental samples. The aim of this study was to...

Exercise, diet and skeletal muscle gene expression (2002)

Hargreaves, Mark, Cameron-Smith, David

Skeletal muscle, as a consequence of its mass and great capacity for altered metabolism, has a major impact on whole-body metabolic homeostasis and is capable of remarkable adaptation in response to...

Exercise training increases lipid metabolism gene expression in human skeletal muscle (2002)

Tunstall, Rebecca J., Mehan, Kate A., Wadley, Glenn D., Collier, Greg, Bonen, Arend, Hargreaves, Mark, ...

The effects of a single bout of exercise and exercise training on the expression of genes necessary for the transport and beta -oxidation of fatty acids (FA), together with the gene expression of...

Amino acids and endurance exercise (2001)

Hargreaves, Mark, Snow, Rodney

Provides information on a study which examined the role of amino acids during endurance exercise and the implications for sports nutrition and performance. Description of amino acid utilization...

Adrenaline increases skeletal muscle glycogenolysis, pyruvate dehydrogenase activation and carbohydrate oxidation during moderate exercise in humans (2001)

Watt, Matthew J., Howlett, Kirsten, Febbraio, Mark A., Spriet, Lawrence L., Hargreaves, Mark

# 1. To evaluate the role of adrenaline in regulating carbohydrate metabolism during moderate exercise, 10 moderately trained men completed two 20 min exercise bouts at 58 ± 2 % peak pulmonary...

Differential effects of exercise on insulin-signaling gene expression in human skeletal muscle. (2001)

Wadley, Glenn, Tunstall, Rebecca, Sanigorski, Andrew, Collier, Gregory, Hargreaves, Mark, Cameron-Smith, David

Skeletal muscle insulin sensitivity is enhanced after acute exercise and short-term endurance training. We investigated the impact of exercise on the gene expression of key insulin-signaling proteins...

Effect of prior exercise on glucose metabolism in trained men. (2001)

Rose, Adam, Howlett, Kirsten, King, Douglas, Hargreaves, Mark

Several studies have demonstrated that oral glucose tolerance is impaired in the immediate postexercise period. A double-tracer technique was used to examine glucose kinetics during a 2-h oral...

Carbohydrate metabolism during exercise in females: effect of reduced fat availability (2001)

Howlett, Kirsten, Spriet, Lawrence, Hargreaves, Mark

This study examined the effect of reduced plasma free fatty acid (FFA) availability on carbohydrate metabolism during exercise. Six untrained women cycled for 60 minutes at approximately 58% of...

Amino acids and endurance exercise (2001)

Hargreaves, Mark, Snow, Rodney

Provides information on a study which examined the role of amino acids during endurance exercise and the implications for sports nutrition and performance. Description of amino acid utilization...

Effect of prior exercise on glucose metabolism in trained men. (2001)

Rose, Adam, Howlett, Kirsten, King, Douglas, Hargreaves, Mark

Several studies have demonstrated that oral glucose tolerance is impaired in the immediate postexercise period. A double-tracer technique was used to examine glucose kinetics during a 2-h oral...

Adrenaline increases skeletal muscle glycogenolysis, pyruvate dehydrogenase activation and carbohydrate oxidation during moderate exercise in humans (2001)

Watt, Matthew J., Howlett, Kirsten, Febbraio, Mark A., Spriet, Lawrence L., Hargreaves, Mark

# 1. To evaluate the role of adrenaline in regulating carbohydrate metabolism during moderate exercise, 10 moderately trained men completed two 20 min exercise bouts at 58 ± 2 % peak pulmonary...

Carbohydrate metabolism during exercise in females: effect of reduced fat availability (2001)

Howlett, Kirsten, Spriet, Lawrence, Hargreaves, Mark

This study examined the effect of reduced plasma free fatty acid (FFA) availability on carbohydrate metabolism during exercise. Six untrained women cycled for 60 minutes at approximately 58% of...

Differential effects of exercise on insulin-signaling gene expression in human skeletal muscle. (2001)

Wadley, Glenn, Tunstall, Rebecca, Sanigorski, Andrew, Collier, Gregory, Hargreaves, Mark, Cameron-Smith, David

Skeletal muscle insulin sensitivity is enhanced after acute exercise and short-term endurance training. We investigated the impact of exercise on the gene expression of key insulin-signaling proteins...

Effect of exercise on protein kinase C activity and localization in human skeletal muscle

Rose, Adam J, Michell, Belinda J, Kemp, Bruce E, Hargreaves, Mark

To investigate the effect of exercise on protein kinase C (PKC) activity and localization in human skeletal muscle, eight healthy men performed cycle ergometer exercise for 40 min at 76 ± 1% the...

Effect of exercise on protein kinase C activity and localization in human skeletal muscle

Rose, Adam J, Michell, Belinda J, Kemp, Bruce E, Hargreaves, Mark

To investigate the effect of exercise on protein kinase C (PKC) activity and localization in human skeletal muscle, eight healthy men performed cycle ergometer exercise for 40 min at 76 ± 1% the...

Adrenaline increases skeletal muscle glycogenolysis, pyruvate dehydrogenase activation and carbohydrate oxidation during moderate exercise in humans

Watt, Matthew J, Howlett, Kirsten F, Febbraio, Mark A, Spriet, Lawrence L, Hargreaves, Mark

To evaluate the role of adrenaline in regulating carbohydrate metabolism during moderate exercise, 10 moderately trained men completed two 20 min exercise bouts at 58 ± 2 % peak pulmonary oxygen...

Carbohydrate ingestion reduces skeletal muscle acetylcarnitine availability but has no effect on substrate phosphorylation at the onset of exercise in man

Watt, Matthew J, Heigenhauser, G J F, Stellingwerff, Trent, Hargreaves, Mark, Spriet, Lawrence L

This study investigated the effect of reduced acetylcarnitine availability on oxidative metabolism during the transition from rest to steady-state exercise. Eight male subjects completed two...

Exercise increases Ca2+–calmodulin-dependent protein kinase II activity in human skeletal muscle

Rose, Adam J, Hargreaves, Mark

There is evidence in rodents that Ca2+-calmodulin-dependent protein kinase II (CaMKII) activity is higher in contracting skeletal muscle, and this kinase may regulate skeletal muscle function and...