Derudder, Emmanuel, Cadera, Emily J., Val, J. Christoph, Wang, Jing, Fox, Casey J., Zha, Shan, ...
By genetically ablating I kappa B kinase (IKK)-mediated activation of the transcription factor NF-kappa B in the B cell lineage and by analyzing a mouse mutant in which immunoglobulin...
The effects of c-Abl mutation on developing B cell differentiation and survival (2009)
Brightbill, Hans, Schlissel, Mark S.
c-Abl is a widely expressed Src family protein tyrosine kinase that is activated by chromosomal translocation in certain human leukemias. While shown in various experimental systems to regulate cell...
RAG2's non-core domain contributes to the ordered regulation of V(D)J recombination (2008)
Curry, John D., Schlissel, Mark S.
Variable (diversity) joining [V(D)J] recombination of immune gene loci proceeds in an ordered manner with D to J portions recombining first and then an upstream V joins that recombinant. We present...
Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells (2005)
Lina E. Tze, Brian R. Schram, Kong-Peng Lam, Kristin A. Hogquist, Keli L. Hippen, Jiabin Liu, ...
Gene rearrangement is a hallmark of B cell maturation. By interrupting basal cell signaling through the rearranged IgM receptor, immature B cells "back-differentiate" to an earlier stage in their...
Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells (2005)
Lina E. Tze, Brian R. Schram, Kong-Peng Lam, Kristin A. Hogquist, Keli L. Hippen, Jiabin Liu, ...
In developing B lymphocytes, a successful V(D)J heavy chain (HC) immunoglobulin (Ig) rearrangement establishes HC allelic exclusion and signals pro-B cells to advance in development to the pre-B...
Borghesi, Lisa A., Hsu, Lih-Yun, Miller, Juli P., Anderson, Michael, Herzenberg, Leonard A., Herzenberg, Leonore A., ...
Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during...
Muljo, Stefan A., Schlissel, Mark S.
The pre‐BCR consists of Ig µ protein, the product of a heavy chain gene assembled by V(D)J recombination in pro‐B cells, the surrogate light chains Vpre‐B and λ5, and the signaling...
Dillon, Stacey R., Schlissel, Mark S.
Jak‐3 is a non‐receptor tyrosine kinase that plays an important role in coordinating signals received through a wide range of cytokine receptors, including the IL‐7 receptor...
Target DNA chromatinization modulates nicking by L1 endonuclease (2001)
Cost, Gregory J., Golding, Amit, Schlissel, Mark S., Boeke, Jef D.
L1 elements are human transposons which replicate via an RNA intermediate. At least 15% of the human genome is composed of L1 sequence. An important initial step in the transposition reaction...
Hempel, William M., Stanhope-Baker, Patricia, Mathieu, Noëlle, Huang, Fang, Schlissel, Mark S., Ferrier, Pierre
Schlissel, Mark S., Corcoran, Lynn M., Baltimore, David
Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene...
Target DNA chromatinization modulates nicking by L1 endonuclease
Cost, Gregory J., Golding, Amit, Schlissel, Mark S., Boeke, Jef D.
L1 elements are human transposons which replicate via an RNA intermediate. At least 15% of the human genome is composed of L1 sequence. An important initial step in the transposition reaction is...
Distinct Factors Regulate the Murine RAG-2 Promoter in B- and T-Cell Lines
Lauring, Josh, Schlissel, Mark S.
The recombination activating genes RAG-1 and RAG-2 are expressed in a lymphoid-cell-specific and developmentally regulated fashion. To understand the transcriptional basis for this regulation, we...
Pro-B-Cell-Specific Transcription and Proapoptotic Function of Protein Kinase Cη
Morrow, Theresa A., Muljo, Stefan A., Zhang, Jun, Hardwick, J. Marie, Schlissel, Mark S.
Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes,...
Wang, Qian-Fei, Lauring, Josh, Schlissel, Mark S.
The RAG-2 gene encodes a component of the V(D)J recombinase which is essential for the assembly of antigen receptor genes in B and T lymphocytes. Previously, we reported that the transcription factor...
Structure of Nonhairpin Coding-End DNA Breaks in Cells Undergoing V(D)J Recombination
The V(D)J recombinase recognizes a pair of immunoglobulin or T-cell receptor gene segments flanked by recombination signal sequences and introduces double-strand breaks, generating two signal ends...
Hempel, William M., Stanhope-Baker, Patricia, Mathieu, Noëlle, Huang, Fang, Schlissel, Mark S., Ferrier, Pierre
Deletion of the TCRβ transcriptional enhancer (Eβ) results in nearly complete inhibition of V(D)J recombination at the TCRβ locus and a block in αβ T cell development. This result, along with...
Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells
Tze, Lina E, Schram, Brian R, Lam, Kong-Peng, Hogquist, Kristin A, Hippen, Keli L, Liu, Jiabin, ...
In developing B lymphocytes, a successful V(D)J heavy chain (HC) immunoglobulin (Ig) rearrangement establishes HC allelic exclusion and signals pro-B cells to advance in development to the pre-B...
Lazorchak, Adam S., Schlissel, Mark S., Zhuang, Yuan
The immunoglobulin kappa light chain (Igκ) locus is regulated in a lineage- and stage-specific manner during B-cell development. The highly restricted timing of V to J gene recombination at the...
Target DNA chromatinization modulates nicking by L1 endonuclease
Cost, Gregory J., Golding, Amit, Schlissel, Mark S., Boeke, Jef D.
L1 elements are human transposons which replicate via an RNA intermediate. At least 15% of the human genome is composed of L1 sequence. An important initial step in the transposition reaction is...
Distinct Factors Regulate the Murine RAG-2 Promoter in B- and T-Cell Lines
Lauring, Josh, Schlissel, Mark S.
The recombination activating genes RAG-1 and RAG-2 are expressed in a lymphoid-cell-specific and developmentally regulated fashion. To understand the transcriptional basis for this regulation, we...
Pro-B-Cell-Specific Transcription and Proapoptotic Function of Protein Kinase Cη
Morrow, Theresa A., Muljo, Stefan A., Zhang, Jun, Hardwick, J. Marie, Schlissel, Mark S.
Using a subtractive cloning scheme on cDNA prepared from primary pro-B and pre-B cells, we identified several genes whose products regulate apoptosis. We further characterized one of these genes,...
Wang, Qian-Fei, Lauring, Josh, Schlissel, Mark S.
The RAG-2 gene encodes a component of the V(D)J recombinase which is essential for the assembly of antigen receptor genes in B and T lymphocytes. Previously, we reported that the transcription factor...
Structure of Nonhairpin Coding-End DNA Breaks in Cells Undergoing V(D)J Recombination
The V(D)J recombinase recognizes a pair of immunoglobulin or T-cell receptor gene segments flanked by recombination signal sequences and introduces double-strand breaks, generating two signal ends...
Hempel, William M., Stanhope-Baker, Patricia, Mathieu, Noëlle, Huang, Fang, Schlissel, Mark S., Ferrier, Pierre
Deletion of the TCRβ transcriptional enhancer (Eβ) results in nearly complete inhibition of V(D)J recombination at the TCRβ locus and a block in αβ T cell development. This result, along with...
Basal Immunoglobulin Signaling Actively Maintains Developmental Stage in Immature B Cells
Tze, Lina E, Schram, Brian R, Lam, Kong-Peng, Hogquist, Kristin A, Hippen, Keli L, Liu, Jiabin, ...
In developing B lymphocytes, a successful V(D)J heavy chain (HC) immunoglobulin (Ig) rearrangement establishes HC allelic exclusion and signals pro-B cells to advance in development to the pre-B...
Lazorchak, Adam S., Schlissel, Mark S., Zhuang, Yuan
The immunoglobulin kappa light chain (Igκ) locus is regulated in a lineage- and stage-specific manner during B-cell development. The highly restricted timing of V to J gene recombination at the...
Pedraza-Alva, Gustavo, Koulnis, Miroslav, Charland, Colette, Thornton, Tina, Clements, James L, Schlissel, Mark S, ...
Delay of cell cycle progression in response to double-strand DNA breaks (DSBs) is critical to allow time for DNA repair and prevent cellular transformation. Here, we show that the p38...
Borghesi, Lisa, Hsu, Lih-Yun, Miller, Juli P., Anderson, Michael, Herzenberg, Leonard, Herzenberg, Leonore, ...
Expression of V(D)J recombinase activity in developing lymphocytes is absolutely required for initiation of V(D)J recombination at antigen receptor loci. However, little is known about when during...
Pax5 Activates Immunoglobulin Heavy Chain V to DJ Rearrangement in Transgenic Thymocytes
Hsu, Lih-Yun, Liang, Hong-Erh, Johnson, Kristen, Kang, Chulho, Schlissel, Mark S.
Mice deficient for the B cell–restricted transcription factor Pax5 show a defect in the VH to DJH rearrangement step of immunoglobulin heavy chain gene assembly even though the expression of the...
Chromosomal reinsertion of broken RSS ends during T cell development
Curry, John D., Schulz, Danae, Guidos, Cynthia J., Danska, Jayne S., Nutter, Lauryl, Nussenzweig, Andre, ...
The V(D)J recombinase catalyzes DNA transposition and translocation both in vitro and in vivo. Because lymphoid malignancies contain chromosomal translocations involving antigen receptor and...
Schlissel, Mark S., Durum, Scott D., Muegge, Kathrin
Defects in the interleukin (IL)-7 signal transduction pathway lead to severe immunodeficiency in humans and in mice. In IL-7 receptor–deficient (IL-7R−/−) mice, lymphoid precursors show a...
Constantinescu, Andrei, Schlissel, Mark S.
The process of V(D)J recombination is crucial for regulating the development of B cells and for determining their eventual antigen specificity. Here we assess the developmental regulation of the...
Bates, Jamie Geier, Cado, Dragana, Nolla, Hector, Schlissel, Mark S.
Complete IgHC gene rearrangement occurs only in B cells in a stage-specific and ordered manner. We used gene targeting to reposition a distal VH gene segment to a region just 5′ of the DH gene...
RAG2's non-core domain contributes to the ordered regulation of V(D)J recombination
Curry, John D., Schlissel, Mark S.
Variable (diversity) joining [V(D)J] recombination of immune gene loci proceeds in an ordered manner with D to J portions recombining first and then an upstream V joins that recombinant. We present...
Amin, Rupesh H., Cado, Dragana, Nolla, Hector, Huang, Dan, Shinton, Susan A., Zhou, Yan, ...
Allelic exclusion of Ig gene expression is necessary to limit the number of functional receptors to one per B cell. The mechanism underlying allelic exclusion is unknown. Because germline...