Role of the anion nitrite in ischemia-reperfusion cytoprotection and therapeutics (2007)
Dezfulian, Cameron, Raat, Nicolaas, Shiva, Sruti, Gladwin, Mark T.
The anion nitrite (NO2−) constitutes a biochemical reservoir for nitric oxide (NO). Nitrite reduction to NO may be catalyzed by hemoglobin, myoglobin or other metal-containing enzymes and occurs at...
Gladwin, Mark T., Shelhamer, James H., Schechter, Alan N., Pease-Fye, Margaret E., Waclawiw, Myron A., Panza, Julio A., ...
To determine the relative contributions of endothelial-derived nitric oxide (NO) vs. intravascular nitrogen oxide species in the regulation of human blood flow, we simultaneously measured forearm...
Gladwin, Mark T., Ognibene, Frederick P., Pannell, Lewis K., Nichols, James S., Pease-Fye, Margaret E., Shelhamer, James H., ...
To quantify the reactions of nitric oxide (NO) with hemoglobin under physiological conditions and to test models of NO transport on hemoglobin, we have developed an assay to measure NO–hemoglobin...
Oxygen radical inhibition of nitric oxide-dependent vascular function in sickle cell disease
Aslan, Mutay, Ryan, Thomas M., Adler, Brian, Townes, Tim M., Parks, Dale A., Thompson, J. Anthony, ...
Plasma xanthine oxidase (XO) activity was defined as a source of enhanced vascular superoxide (O\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts}...
Cokic, Vladan P., Smith, Reginald D., Beleslin-Cokic, Bojana B., Njoroge, Joyce M., Miller, Jeffery L., Gladwin, Mark T., ...
Hydroxyurea treatment of patients with sickle-cell disease increases fetal hemoglobin (HbF), which reduces hemoglobin S polymerization and clinical complications. Despite its use in the treatment of...
Cannon, Richard O., Schechter, Alan N., Panza, Julio A., Ognibene, Frederick P., Pease-Fye, Margaret E., Waclawiw, Myron A., ...
Nitric oxide (NO) may be stabilized by binding to hemoglobin, by nitrosating thiol-containing plasma molecules, or by conversion to nitrite, all reactions potentially preserving its bioactivity in...
Xu, Xiuli, Cho, Man, Spencer, Netanya Y., Patel, Neil, Huang, Zhi, Shields, Howard, ...
Nitric oxide has been proposed to be transported by hemoglobin as a third respiratory gas and to elicit vasodilation by an oxygen-linked (allosteric) mechanism. For hemoglobin to transport nitric...
Haldane, hot dogs, halitosis, and hypoxic vasodilation: the emerging biology of the nitrite anion
While it has long been known that the reduction of nitrite to nitric oxide (NO) forms iron-nitrosyl-myoglobin and is the basis of meat curing, a greater biological activity of the nitrite anion has...
Gladwin, Mark T., Schechter, Alan N., Shelhamer, James H., Pannell, Lewis K., Conway, Deirdre A., Hrinczenko, Borys W., ...
Nitric oxide (NO) inhalation has been reported to increase the oxygen affinity of sickle cell erythrocytes. Also, proposed allosteric mechanisms for hemoglobin, based on S-nitrosation of β-chain...
Biological activity of nitric oxide in the plasmatic compartment
Wang, Xunde, Tanus-Santos, Jose E., Reiter, Christopher D., Dejam, Andre, Shiva, Sruti, Smith, Reginald D., ...
There exist reaction products of nitric oxide (NO) with blood that conserve its bioactivity and transduce an endocrine vasomotor function under certain conditions. Although S-nitrosated albumin has...
Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver
Duranski, Mark R., Greer, James J.M., Dejam, Andre, Jaganmohan, Sathya, Hogg, Neil, Langston, William, ...
Nitrite represents a circulating and tissue storage form of NO whose bioactivation is mediated by the enzymatic action of xanthine oxidoreductase, nonenzymatic disproportionation, and reduction by...
Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
Huang, Zhi, Shiva, Sruti, Kim-Shapiro, Daniel B., Patel, Rakesh P., Ringwood, Lorna A., Irby, Cynthia E., ...
Hypoxic vasodilation is a fundamental, highly conserved physiological response that requires oxygen and/or pH sensing coupled to vasodilation. While this process was first characterized more than 80...
Minneci, Peter C., Deans, Katherine J., Zhi, Huang, Yuen, Peter S.T., Star, Robert A., Banks, Steven M., ...
During intravascular hemolysis in human disease, vasomotor tone and organ perfusion may be impaired by the increased reactivity of cell-free plasma hemoglobin (Hb) with NO. We experimentally produced...
In this issue of the JCI, Stasch and colleagues suggest that a novel drug, BAY 58-2667, potently activates a pool of oxidized and heme-free soluble guanylyl cyclase (sGC; see the related article...
Gladwin, Mark T., Shelhamer, James H., Schechter, Alan N., Pease-Fye, Margaret E., Waclawiw, Myron A., Panza, Julio A., ...
To determine the relative contributions of endothelial-derived nitric oxide (NO) vs. intravascular nitrogen oxide species in the regulation of human blood flow, we simultaneously measured forearm...
Gladwin, Mark T., Ognibene, Frederick P., Pannell, Lewis K., Nichols, James S., Pease-Fye, Margaret E., Shelhamer, James H., ...
To quantify the reactions of nitric oxide (NO) with hemoglobin under physiological conditions and to test models of NO transport on hemoglobin, we have developed an assay to measure NO–hemoglobin...
Oxygen radical inhibition of nitric oxide-dependent vascular function in sickle cell disease
Aslan, Mutay, Ryan, Thomas M., Adler, Brian, Townes, Tim M., Parks, Dale A., Thompson, J. Anthony, ...
Plasma xanthine oxidase (XO) activity was defined as a source of enhanced vascular superoxide (O\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts}...
Cokic, Vladan P., Smith, Reginald D., Beleslin-Cokic, Bojana B., Njoroge, Joyce M., Miller, Jeffery L., Gladwin, Mark T., ...
Hydroxyurea treatment of patients with sickle-cell disease increases fetal hemoglobin (HbF), which reduces hemoglobin S polymerization and clinical complications. Despite its use in the treatment of...
Cannon, Richard O., Schechter, Alan N., Panza, Julio A., Ognibene, Frederick P., Pease-Fye, Margaret E., Waclawiw, Myron A., ...
Nitric oxide (NO) may be stabilized by binding to hemoglobin, by nitrosating thiol-containing plasma molecules, or by conversion to nitrite, all reactions potentially preserving its bioactivity in...
Xu, Xiuli, Cho, Man, Spencer, Netanya Y., Patel, Neil, Huang, Zhi, Shields, Howard, ...
Nitric oxide has been proposed to be transported by hemoglobin as a third respiratory gas and to elicit vasodilation by an oxygen-linked (allosteric) mechanism. For hemoglobin to transport nitric...
Haldane, hot dogs, halitosis, and hypoxic vasodilation: the emerging biology of the nitrite anion
While it has long been known that the reduction of nitrite to nitric oxide (NO) forms iron-nitrosyl-myoglobin and is the basis of meat curing, a greater biological activity of the nitrite anion has...
Gladwin, Mark T., Schechter, Alan N., Shelhamer, James H., Pannell, Lewis K., Conway, Deirdre A., Hrinczenko, Borys W., ...
Nitric oxide (NO) inhalation has been reported to increase the oxygen affinity of sickle cell erythrocytes. Also, proposed allosteric mechanisms for hemoglobin, based on S-nitrosation of β-chain...
Biological activity of nitric oxide in the plasmatic compartment
Wang, Xunde, Tanus-Santos, Jose E., Reiter, Christopher D., Dejam, Andre, Shiva, Sruti, Smith, Reginald D., ...
There exist reaction products of nitric oxide (NO) with blood that conserve its bioactivity and transduce an endocrine vasomotor function under certain conditions. Although S-nitrosated albumin has...
Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver
Duranski, Mark R., Greer, James J.M., Dejam, Andre, Jaganmohan, Sathya, Hogg, Neil, Langston, William, ...
Nitrite represents a circulating and tissue storage form of NO whose bioactivation is mediated by the enzymatic action of xanthine oxidoreductase, nonenzymatic disproportionation, and reduction by...
Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
Huang, Zhi, Shiva, Sruti, Kim-Shapiro, Daniel B., Patel, Rakesh P., Ringwood, Lorna A., Irby, Cynthia E., ...
Hypoxic vasodilation is a fundamental, highly conserved physiological response that requires oxygen and/or pH sensing coupled to vasodilation. While this process was first characterized more than 80...
Minneci, Peter C., Deans, Katherine J., Zhi, Huang, Yuen, Peter S.T., Star, Robert A., Banks, Steven M., ...
During intravascular hemolysis in human disease, vasomotor tone and organ perfusion may be impaired by the increased reactivity of cell-free plasma hemoglobin (Hb) with NO. We experimentally produced...
In this issue of the JCI, Stasch and colleagues suggest that a novel drug, BAY 58-2667, potently activates a pool of oxidized and heme-free soluble guanylyl cyclase (sGC; see the related article...
Erythrocytes are the major intravascular storage sites of nitrite in human blood
Dejam, André, Hunter, Christian J., Pelletier, Mildred M., Hsu, Lewis L., Machado, Roberto F., Shiva, Sruti, ...
Plasma levels of nitrite ions have been used as an index of nitric oxide synthase (NOS) activity in vivo. Recent data suggest that nitrite is a potential intravascular repository for nitric oxide...
Hypoxia, red blood cells, and nitrite regulate NO-dependent hypoxic vasodilation
Crawford, Jack H., Isbell, T. Scott, Huang, Zhi, Shiva, Sruti, Chacko, Balu K., Schechter, Alan N., ...
Local vasodilation in response to hypoxia is a fundamental physiologic response ensuring oxygen delivery to tissues under metabolic stress. Recent studies identify a role for the red blood cell...
Kato, Gregory J., McGowan, Vicki, Machado, Roberto F., Little, Jane A., Taylor, James, Morris, Claudia R., ...
Pulmonary hypertension is prevalent in adult patients with sickle cell disease and is strongly associated with early mortality and markers of hemolysis, in particular, serum lactate dehydrogenase...
Shiva, Sruti, Sack, Michael N., Greer, James J., Duranski, Mark, Ringwood, Lorna A., Burwell, Lindsay, ...
Nitrite (NO2−) is an intrinsic signaling molecule that is reduced to NO during ischemia and limits apoptosis and cytotoxicity at reperfusion in the mammalian heart, liver, and brain. Although the...
Hsu, Lewis L., Champion, Hunter C., Campbell-Lee, Sally A., Bivalacqua, Trinity J., Manci, Elizabeth A., Diwan, Bhalchandra A., ...
Pulmonary hypertension is a highly prevalent complication of sickle cell disease and is a strong risk factor for early mortality. However, the pathophysiologic mechanisms leading to pulmonary...
Taylor, James G., Nolan, Vikki G., Mendelsohn, Laurel, Kato, Gregory J., Gladwin, Mark T., Steinberg, Martin H.
Anthi, Anastasia, Machado, Roberto F., Jison, Maria L., Taveira-DaSilva, Angelo M., Rubin, Lewis J., Hunter, Lori, ...
Rationale: Although pulmonary hypertension (PH) is a common complication of sickle cell disease (SCD) associated with high mortality, there exist few data characterizing hemodynamics and...
Villagra, José, Shiva, Sruti, Hunter, Lori A., Machado, Roberto F., Gladwin, Mark T., Kato, Gregory J.
Increased platelet activation is recognized in patients with sickle cell disease (SCD), but its pathogenesis and clinical relevance remain uncertain. Pulmonary arterial hypertension (PAH), an...
A network model to predict the risk of death in sickle cell disease
Sebastiani, Paola, Nolan, Vikki G., Baldwin, Clinton T., Abad-Grau, Maria M., Wang, Ling, Adewoye, Adeboye H., ...
Modeling the complexity of sickle cell disease pathophysiology and severity is difficult. Using data from 3380 patients accounting for all common genotypes of sickle cell disease, Bayesian network...
Hendgen-Cotta, Ulrike B., Merx, Marc W., Shiva, Sruti, Schmitz, Joel, Becher, Stefanie, Klare, Johann P., ...
The nitrite anion is reduced to nitric oxide (NO•) as oxygen tension decreases. Whereas this pathway modulates hypoxic NO• signaling and mitochondrial respiration and limits myocardial infarction...
Minniti, Caterina P., Sable, Craig, Campbell, Andrew, Rana, Sohail, Ensing, Gregory, Dham, Niti, ...
An elevated echocardiography-determined tricuspid regurgitant jet velocity predicts high systolic pulmonary artery pressure and early mortality in adults with sickle cell disease. The study provides...
Raghavachari, Nalini, Xu, Xiuli, Munson, Peter J., Gladwin, Mark T.
Global transcriptome analysis of whole blood RNA using microarrays has been proven to be challenging due to the high abundance of globin transcripts that constitute 70% of whole blood mRNA. This is a...
Minneci, Peter C., Deans, Katherine J., Shiva, Sruti, Zhi, Huang, Banks, Steven M., Kern, Steven, ...
Hemoglobin (Hb) potently inactivates the nitric oxide (NO) radical via a dioxygenation reaction forming nitrate (NO3−). This inactivation produces endothelial dysfunction during hemolytic...
The functional nitrite reductase activity of the heme-globins
Gladwin, Mark T., Kim-Shapiro, Daniel B.
Hemoglobin and myoglobin are among the most extensively studied proteins, and nitrite is one of the most studied small molecules. Recently, multiple physiologic studies have surprisingly revealed...
Nitrite Reductase Activity of Cytochrome c*
Basu, Swati, Azarova, Natalia A., Font, Michael D., King, S. Bruce, Hogg, Neil, Gladwin, Mark T., ...
Small increases in physiological nitrite concentrations have now been shown to mediate a number of biological responses, including hypoxic vasodilation, cytoprotection after ischemia/reperfusion, and...