-Catenin is a Nek2 substrate involved in centrosome separation (2008)
Bahmanyar, Shirin, Kaplan, Daniel D., DeLuca, Jennifer G., Giddings, Thomas H., OToole, Eileen T., Winey, Mark, ...
β-Catenin plays important roles in cell adhesion and gene transcription, and has been shown recently to be essential for the establishment of a bipolar mitotic spindle. Here we show that β-catenin...
BELIAKOVA-BETHELL, NADEJDA, BECKHAM, CARLA, GIDDINGS, THOMAS H., WINEY, MARK, PARKER, ROY, SANDMEYER, SUZANNE
Retroviruses and retrotransposons assemble intracellular immature core particles around a RNA genome, and nascent particles collect in association with membranes or as intracellular clusters. How and...
Nels C. Elde, Garry Morgan, Mark Winey, Linda Sperling, Aaron P. Turkewitz
Ciliates, although single-celled organisms, contain numerous subcellular structures and pathways usually associated with metazoans. How this cell biological complexity relates to the evolution of...
Nels C Elde, Garry Morgan, Mark Winey, Linda Sperling, Aaron Turkewitz
Ciliates, though single-celled organisms, contain numerous subcellular structures and pathways usually associated with metazoans. How this cell biological complexity relates to the evolution of...
Mark Winey, Defne Yarar, Thomas H. Giddings, David N. Mastronarde
number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of...
Straight, Paul D., Giddings, Thomas H., Winey, Mark
Sporulation in yeast requires that a modified form of chromosome segregation be coupled to the development of a specialized cell type, a process akin to gametogenesis. Mps1p is a dual-specificity...
MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy
Mob1p is an essential Saccharomyces cerevisiae protein, identified from a two-hybrid screen, that binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint...
New Alleles of the Yeast MPS1 Gene Reveal Multiple Requirements in Spindle Pole Body Duplication
In Saccharomyces cerevisiae, the Mps1p protein kinase is critical for both spindle pole body (SPB) duplication and the mitotic spindle assembly checkpoint. The mps1–1 mutation causes failure early...
O’Toole, Eileen T., Winey, Mark, McIntosh, J. Richard
The spindle pole body (SPB) is the major microtubule-organizing center of budding yeast and is the functional equivalent of the centrosome in higher eukaryotic cells. We used fast-frozen,...
Jones, Michele H., Bachant, Jeffrey B., Castillo, Andrea R., Giddings, Thomas H., Winey, Mark
We have identified a mutant allele of the DAM1 gene in a screen for mutations that are lethal in combination with the mps1-1 mutation. MPS1 encodes an essential protein kinase that is required for...
McBratney, Susan, Monterrosa, Antonio, Byers, Breck, Mann, Carl, Winey, Mark
The MPS2 (monopolar spindle two) gene is one of several genes required for the proper execution of spindle pole body (SPB) duplication in the budding yeast Saccharomyces cerevisiae (Winey et al.,...
Winey, Mark, Yarar, Defne, Giddings, Thomas H., Mastronarde, David N.
The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of...
Cnm67p Is a Spacer Protein of the Saccharomyces cerevisiae Spindle Pole Body Outer Plaque
Schaerer, Florian, Morgan, Garry, Winey, Mark, Philippsen, Peter
In Saccharomyces cerevisiae, the spindle pole body (SPB) is the functional homolog of the mammalian centrosome, responsible for the organization of the tubulin cytoskeleton. Cytoplasmic (astral)...
Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle
Jones, Michele H., He, Xiangwei, Giddings, Thomas H., Winey, Mark
During mitosis, replicated chromosomes are separated to daughter cells by the microtubule-based mitotic spindle. Chromosomes attach to the mitotic spindle through specialized DNA/protein structures...
Saccharomyces cerevisiae Mob1p Is Required for Cytokinesis and Mitotic Exit
Luca, Francis C., Mody, Manali, Kurischko, Cornelia, Roof, David M., Giddings, Thomas H., Winey, Mark
The Saccharomyces cerevisiae mitotic exit network (MEN) is a conserved set of genes that mediate the transition from mitosis to G1 by regulating mitotic cyclin degradation and the inactivation of...
DBF2 Protein Kinase Binds to and Acts through the Cell Cycle-Regulated MOB1 Protein†
Komarnitsky, Svetlana I., Chiang, Yueh-Chin, Luca, Francis C., Chen, Junji, Toyn, Jeremy H., Winey, Mark, ...
The DBF2 gene of the budding yeast Saccharomyces cerevisiae encodes a cell cycle-regulated protein kinase that plays an important role in the telophase/G1 transition. As a component of the...
Burns, C. Geoffrey, Ohi, Ryoma, Mehta, Sapna, O’Toole, Eileen T., Winey, Mark, Clark, Tyson A., ...
Genetic and biochemical studies of Schizosaccharomyces pombe and Saccharomyces cerevisiae have identified gene products that play essential functions in both pre-mRNA splicing and cell cycle control....
Piecemeal Microautophagy of Nucleus in Saccharomyces cerevisiae
Roberts, Paul, Moshitch-Moshkovitz, Sharon, Kvam, Erik, O'Toole, Eileen, Winey, Mark, Goldfarb, David S.
Nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae are formed through specific interactions between Vac8p on the vacuole membrane and Nvj1p in the nuclear envelope. Herein, we report that NV...
Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression
Fisk, Harold A., Mattison, Christopher P., Winey, Mark
The mitotic spindle is essential for the maintenance of genetic stability, and in budding yeast its assembly and function depend on the Mps1 protein kinase. Mps1p is required for centrosome...
Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae
Park, Chong J., Song, Sukgil, Giddings, Thomas H., Ro, Hyeon-Su, Sakchaisri, Krisada, Park, Jung-Eun, ...
The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we...
Lau, Corine K., Giddings, Thomas H., Winey, Mark
Both the spindle pole body (SPB) and the nuclear pore complex (NPC) are essential organelles embedded in the nuclear envelope throughout the life cycle of the budding yeast Saccharomyces cerevisiae....
Yoder, Tennessee J., McElwain, Mark A., Francis, Susan E., Bagley, Joy, Muller, Eric G.D., Pak, Brian, ...
The spindle pole body (SPB) is the microtubule organizing center in Saccharomyces cerevisiae. An essential task of the SPB is to ensure assembly of the bipolar spindle, which requires a proper...
Stemm-Wolf, Alexander J., Morgan, Garry, Giddings, Thomas H., White, Erin A., Marchione, Robb, McDonald, Heather B., ...
Centrins, small calcium binding EF-hand proteins, function in the duplication of a variety of microtubule organizing centers. These include centrioles in humans, basal bodies in green algae, and...
Elde, Nels C, Morgan, Garry, Winey, Mark, Sperling, Linda, Turkewitz, Aaron P
Ciliates, although single-celled organisms, contain numerous subcellular structures and pathways usually associated with metazoans. How this cell biological complexity relates to the evolution of...
Three-dimensional Ultrastructure of Saccharomyces cerevisiae Meiotic SpindlesD⃞V⃞
Winey, Mark, Morgan, Garry P., Straight, Paul D., Giddings, Thomas H., Mastronarde, David N.
Meiotic chromosome segregation leads to the production of haploid germ cells. During meiosis I (MI), the paired homologous chromosomes are separated. Meiosis II (MII) segregation leads to the...
Araki, Yasuhiro, Lau, Corine K., Maekawa, Hiromi, Jaspersen, Sue L., Giddings, Thomas H., Schiebel, Elmar, ...
The spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However,...
Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components
BELIAKOVA-BETHELL, NADEJDA, BECKHAM, CARLA, GIDDINGS, THOMAS H., WINEY, MARK, PARKER, ROY, SANDMEYER, SUZANNE
Retroviruses and retrotransposons assemble intracellular immature core particles around a RNA genome, and nascent particles collect in association with membranes or as intracellular clusters. How and...
Mutation of either the yeast MPS2 or the NDC1 gene leads to identical spindle pole body (SPB) duplication defects: The newly formed SPB is improperly inserted into the nuclear envelope (NE),...
Straight, Paul D., Giddings, Thomas H., Winey, Mark
Sporulation in yeast requires that a modified form of chromosome segregation be coupled to the development of a specialized cell type, a process akin to gametogenesis. Mps1p is a dual-specificity...
MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy
Mob1p is an essential Saccharomyces cerevisiae protein, identified from a two-hybrid screen, that binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint...
New Alleles of the Yeast MPS1 Gene Reveal Multiple Requirements in Spindle Pole Body Duplication
In Saccharomyces cerevisiae, the Mps1p protein kinase is critical for both spindle pole body (SPB) duplication and the mitotic spindle assembly checkpoint. The mps1–1 mutation causes failure early...
O’Toole, Eileen T., Winey, Mark, McIntosh, J. Richard
The spindle pole body (SPB) is the major microtubule-organizing center of budding yeast and is the functional equivalent of the centrosome in higher eukaryotic cells. We used fast-frozen,...
Jones, Michele H., Bachant, Jeffrey B., Castillo, Andrea R., Giddings, Thomas H., Winey, Mark
We have identified a mutant allele of the DAM1 gene in a screen for mutations that are lethal in combination with the mps1-1 mutation. MPS1 encodes an essential protein kinase that is required for...
McBratney, Susan, Monterrosa, Antonio, Byers, Breck, Mann, Carl, Winey, Mark
The MPS2 (monopolar spindle two) gene is one of several genes required for the proper execution of spindle pole body (SPB) duplication in the budding yeast Saccharomyces cerevisiae (Winey et al.,...
Winey, Mark, Yarar, Defne, Giddings, Thomas H., Mastronarde, David N.
The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of...
Cnm67p Is a Spacer Protein of the Saccharomyces cerevisiae Spindle Pole Body Outer Plaque
Schaerer, Florian, Morgan, Garry, Winey, Mark, Philippsen, Peter
In Saccharomyces cerevisiae, the spindle pole body (SPB) is the functional homolog of the mammalian centrosome, responsible for the organization of the tubulin cytoskeleton. Cytoplasmic (astral)...
Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle
Jones, Michele H., He, Xiangwei, Giddings, Thomas H., Winey, Mark
During mitosis, replicated chromosomes are separated to daughter cells by the microtubule-based mitotic spindle. Chromosomes attach to the mitotic spindle through specialized DNA/protein structures...
Saccharomyces cerevisiae Mob1p Is Required for Cytokinesis and Mitotic Exit
Luca, Francis C., Mody, Manali, Kurischko, Cornelia, Roof, David M., Giddings, Thomas H., Winey, Mark
The Saccharomyces cerevisiae mitotic exit network (MEN) is a conserved set of genes that mediate the transition from mitosis to G1 by regulating mitotic cyclin degradation and the inactivation of...
DBF2 Protein Kinase Binds to and Acts through the Cell Cycle-Regulated MOB1 Protein†
Komarnitsky, Svetlana I., Chiang, Yueh-Chin, Luca, Francis C., Chen, Junji, Toyn, Jeremy H., Winey, Mark, ...
The DBF2 gene of the budding yeast Saccharomyces cerevisiae encodes a cell cycle-regulated protein kinase that plays an important role in the telophase/G1 transition. As a component of the...
Burns, C. Geoffrey, Ohi, Ryoma, Mehta, Sapna, O’Toole, Eileen T., Winey, Mark, Clark, Tyson A., ...
Genetic and biochemical studies of Schizosaccharomyces pombe and Saccharomyces cerevisiae have identified gene products that play essential functions in both pre-mRNA splicing and cell cycle control....
Piecemeal Microautophagy of Nucleus in Saccharomyces cerevisiae
Roberts, Paul, Moshitch-Moshkovitz, Sharon, Kvam, Erik, O'Toole, Eileen, Winey, Mark, Goldfarb, David S.
Nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae are formed through specific interactions between Vac8p on the vacuole membrane and Nvj1p in the nuclear envelope. Herein, we report that NV...
Human Mps1 protein kinase is required for centrosome duplication and normal mitotic progression
Fisk, Harold A., Mattison, Christopher P., Winey, Mark
The mitotic spindle is essential for the maintenance of genetic stability, and in budding yeast its assembly and function depend on the Mps1 protein kinase. Mps1p is required for centrosome...
Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae
Park, Chong J., Song, Sukgil, Giddings, Thomas H., Ro, Hyeon-Su, Sakchaisri, Krisada, Park, Jung-Eun, ...
The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we...
Lau, Corine K., Giddings, Thomas H., Winey, Mark
Both the spindle pole body (SPB) and the nuclear pore complex (NPC) are essential organelles embedded in the nuclear envelope throughout the life cycle of the budding yeast Saccharomyces cerevisiae....
Yoder, Tennessee J., McElwain, Mark A., Francis, Susan E., Bagley, Joy, Muller, Eric G.D., Pak, Brian, ...
The spindle pole body (SPB) is the microtubule organizing center in Saccharomyces cerevisiae. An essential task of the SPB is to ensure assembly of the bipolar spindle, which requires a proper...
Three-dimensional Ultrastructure of Saccharomyces cerevisiae Meiotic SpindlesD⃞V⃞
Winey, Mark, Morgan, Garry P., Straight, Paul D., Giddings, Thomas H., Mastronarde, David N.
Meiotic chromosome segregation leads to the production of haploid germ cells. During meiosis I (MI), the paired homologous chromosomes are separated. Meiosis II (MII) segregation leads to the...
Stemm-Wolf, Alexander J., Morgan, Garry, Giddings, Thomas H., White, Erin A., Marchione, Robb, McDonald, Heather B., ...
Centrins, small calcium binding EF-hand proteins, function in the duplication of a variety of microtubule organizing centers. These include centrioles in humans, basal bodies in green algae, and...
Elde, Nels C, Morgan, Garry, Winey, Mark, Sperling, Linda, Turkewitz, Aaron P
Ciliates, although single-celled organisms, contain numerous subcellular structures and pathways usually associated with metazoans. How this cell biological complexity relates to the evolution of...
Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components
BELIAKOVA-BETHELL, NADEJDA, BECKHAM, CARLA, GIDDINGS, THOMAS H., WINEY, MARK, PARKER, ROY, SANDMEYER, SUZANNE
Retroviruses and retrotransposons assemble intracellular immature core particles around a RNA genome, and nascent particles collect in association with membranes or as intracellular clusters. How and...
Araki, Yasuhiro, Lau, Corine K., Maekawa, Hiromi, Jaspersen, Sue L., Giddings, Thomas H., Schiebel, Elmar, ...
The spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However,...
Mutation of either the yeast MPS2 or the NDC1 gene leads to identical spindle pole body (SPB) duplication defects: The newly formed SPB is improperly inserted into the nuclear envelope (NE),...
Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins
Crasta, Karen, Huang, Phillips, Morgan, Garry, Winey, Mark, Surana, Uttam
In yeast, separation of duplicated spindle pole bodies (SPBs) (centrosomes in higher eukaryotes) is an indispensable step in the assembly of mitotic spindle and is triggered by severing of the bridge...
Jaspersen, Sue L., Martin, Adriana E., Glazko, Galina, Giddings, Thomas H., Morgan, Garry, Mushegian, Arcady, ...
The spindle pole body (SPB) is the sole site of microtubule nucleation in Saccharomyces cerevisiae; yet, details of its assembly are poorly understood. Integral membrane proteins including Mps2...
Kasbek, Christopher, Yang, Ching-Hui, Yusof, Adlina Mohd, Chapman, Heather M., Winey, Mark, Fisk, Harold A.
Supernumerary centrosomes promote the assembly of abnormal mitotic spindles in many human tumors. In human cells, overexpression of the cyclin-dependent kinase (Cdk)2 partner cyclin A during a...
New Tetrahymena basal body protein components identify basal body domain structure
Kilburn, Chandra L., Pearson, Chad G., Romijn, Edwin P., Meehl, Janet B., Giddings, Thomas H., Culver, Brady P., ...
Basal bodies organize the nine doublet microtubules found in cilia. Cilia are required for a variety of cellular functions, including motility and sensing stimuli. Understanding this biochemically...
New Tetrahymena basal body protein components identify basal body domain structure
Kilburn, Chandra L., Pearson, Chad G., Romijn, Edwin P., Meehl, Janet B., Giddings, Thomas H., Culver, Brady P., ...
Schutz, Amy R., Giddings, Thomas H., Steiner, Estelle, Winey, Mark
The MPS1 gene from Saccharomyces cerevisiae encodes an essential protein kinase required for spindle pole body (SPB) duplication and for the mitotic spindle assembly checkpoint. Cells with the mps1-1...
Castillo, Andrea R., Meehl, Janet B., Morgan, Garry, Schutz-Geschwender, Amy, Winey, Mark
Saccharomyces cerevisiae MPS1 encodes an essential protein kinase that has roles in spindle pole body (SPB) duplication and the spindle checkpoint. Previously characterized MPS1 mutants fail in both...
Jaspersen, Sue L., Giddings, Thomas H., Winey, Mark
Accurate duplication of the Saccharomyces cerevisiae spindle pole body (SPB) is required for formation of a bipolar mitotic spindle. We identified mutants in SPB assembly by screening a...
Chial, Heidi J., Rout, Michael P., Giddings, Thomas H., Winey, Mark
We report a novel connection between nuclear pore complexes (NPCs) and spindle pole bodies (SPBs) revealed by our studies of the Saccharomyces cerevisiae NDC1 gene. Although both NPCs and SPBs are...
β-Catenin is a Nek2 substrate involved in centrosome separation
Bahmanyar, Shirin, Kaplan, Daniel D., DeLuca, Jennifer G., Giddings, Thomas H., O’Toole, Eileen T., Winey, Mark, ...
β-Catenin plays important roles in cell adhesion and gene transcription, and has been shown recently to be essential for the establishment of a bipolar mitotic spindle. Here we show that β-catenin...
Gardner, Melissa K., Haase, Julian, Mythreye, Karthikeyan, Molk, Jeffrey N., Anderson, MaryBeth, Joglekar, Ajit P., ...
In budding yeast, the mitotic spindle is comprised of 32 kinetochore microtubules (kMTs) and ∼8 interpolar MTs (ipMTs). Upon anaphase onset, kMTs shorten to the pole, whereas ipMTs increase in...
Basal Body Components Exhibit Differential Protein Dynamics during Nascent Basal Body Assembly
Pearson, Chad G., Giddings, Thomas H., Winey, Mark
Basal bodies organize cilia that are responsible for both mechanical beating and sensation. Nascent basal body assembly follows a series of well characterized morphological events; however, the...
The Two SAS-6 Homologs in Tetrahymena thermophila Have Distinct Functions in Basal Body Assembly
Culver, Brady P., Meehl, Janet B., Giddings, Thomas H., Winey, Mark
Cilia and flagella are structurally and functionally conserved organelles present in basal as well as higher eukaryotes. The assembly of cilia requires a microtubule based scaffold called a basal...