Martin Wiedmann

Publication List Details

Period

1990 - 2008

Number

78

Co-Authors

Short-term genome evolution of Listeria monocytogenesin a non-controlled environment (2008)

Orsi, Renato H, Borowsky, Mark L, Lauer, Peter, Young, Sarah K, Nusbaum, Chad, Galagan, James E, ...

Abstract Background While increasing data on bacterial evolution in controlled environments are available, our understanding of bacterial genome evolution in natural environments is limited. We thus...

Lineage specific recombination rates and microevolution in Listeria monocytogenes (2008)

Den Bakker, Henk C, Didelot, Xavier, Fortes, Esther D, Nightingale, Kendra K, Wiedmann, Martin

Abstract Background The bacterium Listeria monocytogenes is a saprotroph as well as an opportunistic human foodborne pathogen, which has previously been shown to consist of at least two widespread...

Lineage specific recombination rates and microevolution in Listeria monocytogenes (2008)

Den Bakker, Henk C., Didelot, Xavier, Fortes, Esther D, Nightingale, Kendra K, Wiedmann, Martin

Background: The bacterium Listeria monocytogenes is a saprotroph as well as an opportunistic human foodborne pathogen, which has previously been shown to consist of at least two widespread lineages...

Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeria monocytogenes (2008)

Orsi, Renato H, Sun, Qi, Wiedmann, Martin

Abstract Background The genus Listeria includes two closely related pathogenic and non-pathogenic species, L. monocytogenes and L. innocua . L. monocytogenes is an opportunistic human foodborne and...

Short-term genome evolution of Listeria monocytogenes in a non-controlled environment (2008)

Orsi, Renato H., Borowsky, Mark L., Lauer, Peter, Young, Sarah K., Nusbaum, Chad, Galagan, James E., ...

Background: While increasing data on bacterial evolution in controlled environments are available, our understanding of bacterial genome evolution in natural environments is limited. We thus...

BMC Evolutionary Biology (2007)

Renato H Orsi, Qi Sun, Martin Wiedmann

Research article Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeria monocytogenes

Ribotype diversity of Listeria monocytogenes isolates from two salmon processing plants in Norway (2006)

Klæboe, Halvdan, Rosef, Olav, Fortes, Esther, Wiedmann, Martin

The purpose of this study was to use automated ribotyping procedure to track Listeria monocytogenes transmission in the cold smoked fish production chain and to characterize L. monocytogenes subtypes...

Ceftiofur-Resistant Salmonella Strains Isolated from Dairy Farms Represent Multiple Widely Distributed Subtypes That Evolved by Independent Horizontal Gene Transfer (2005)

Alcaine, Samuel D, Sukhnanand, Sharinne S, Warnick, Lorin D, Su, Wan-lin, McGann, Patrick, McDonough, Patrick, ...

Salmonella enterica is a gram-negative, rod shaped bacillus, which inhabits the intestines of mammals, reptiles and birds. In the United States, Salmonella is one of the leading causes of food-borne...

Ceftiofur-Resistant Salmonella Strains Isolated from Dairy Farms Represent Multiple Widely Distributed Subtypes That Evolved by Independent Horizontal Gene Transfer (2005)

Alcaine, Samuel D, Sukhnanand, Sharinne S, Warnick, Lorin D, Su, Wan-lin, McGann, Patrick, McDonough, Patrick, ...

Salmonella enterica is a gram-negative, rod shaped bacillus, which inhabits the intestines of mammals, reptiles and birds. In the United States, Salmonella is one of the leading causes of food-borne...

The mtDNA T8993G (NARP) mutation results in an impairment of oxidative phosphorylation that can be improved by antioxidants (2004)

Mattiazzi, Marina, Vijayvergiya, Chetan, Gajewski, Carl D., DeVivo, Darryl C., Lenaz, Giorgio, Wiedmann, Martin, ...

A T8993G point mutation in the mtDNA results in a Leu156Arg substitution in the MTATP6 subunit of the mitochondrial F1F0-ATPase. The T8993G mutation causes impaired oxidative phosphorylation (OXPHOS)...

The mtDNA T8993G (NARP) mutation results in an impairment of oxidative phosphorylation that can be improved by antioxidants (2004)

Mattiazzi, Marina, Vijayvergiya, Chetan, Gajewski, Carl D., DeVivo, Darryl C., Lenaz, Giorgio, Wiedmann, Martin, ...

A T8993G point mutation in the mtDNA results in a Leu156Arg substitution in the MTATP6 subunit of the mitochondrial F1F0-ATPase. The T8993G mutation causes impaired oxidative phosphorylation (OXPHOS)...

The mtDNA T8993G (NARP) mutation results in an impairment of oxidative phosphorylation that can be improved by antioxidants (2004)

Mattiazzi, Marina, Vijayvergiya, Chetan, Gajewski, Carl D., DeVivo, Darryl C., Lenaz, Giorgio, Wiedmann, Martin, ...

A T8993G point mutation in the mtDNA results in a Leu156Arg substitution in the MTATP6 subunit of the mitochondrial F1F0-ATPase. The T8993G mutation causes impaired oxidative phosphorylation (OXPHOS)...

Mapping of the protein import machinery in the mitochondrial outer membrane by crosslinking of translocation intermediates (1992)

Söllner, Thomas, Rassow, Joachim, Wiedmann, Martin, Schlossmann, Jens, Keil, Petra, Neupert, Walter, ...

Mitochondria contain a complex machinery for the import of nuclear-encoded proteins. Receptor proteins exposed on the outer membrane surface are required for the specific binding of precursor...

The Identification of Proteins in the Proximity of Signal-Anchor Sequences during Their Targeting to and Insertion into the Membrane of the ER (1991)

High, Stephen, Görlich, Dirk, Wiedmann, Martin, Rapoport, Tom A., Dobberstein, Bernhard

Using a photocross-linking approach we have investigated the cytosolic and membrane components involved in the targeting and insertion of signalanchor proteins into the membrane of the ER. The...

The Signal Sequence Receptor Has a Second Subunit and IsPart of a Translocation Complex in the Endoplasmic Reticulum as Probed by Bifunctional Reagents (1990)

Görlich, Dirk, Prehn, Siegfried, Hartmann, Enno, Herz, Joachim, Otto, Albrecht, Kraft, Regine, ...

Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V....

Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase

Ryazanov, Alexey G., Ward, Michael D., Mendola, Charmaine E., Pavur, Karen S., Dorovkov, Maxim V., Wiedmann, Martin, ...

The several hundred members of the eukaryotic protein kinase superfamily characterized to date share a similar catalytic domain structure, consisting of 12 conserved subdomains. Here we report the...

A general mechanism for regulation of access to the translocon: Competition for a membrane attachment site on ribosomes

Möller, Ines, Jung, Martin, Beatrix, Birgitta, Levy, Robert, Kreibich, Gert, Zimmermann, Richard, ...

For proteins to enter the secretory pathway, the membrane attachment site (M-site) on ribosomes must bind cotranslationally to the Sec61 complex present in the endoplasmic reticulum membrane. The...

Automated Ribotyping Using Different Enzymes To Improve Discrimination of Listeria monocytogenes Isolates, with a Particular Focus on Serotype 4b Strains

De Cesare, Alessandra, Bruce, James L., Dambaugh, Timothy R., Guerzoni, Maria E., Wiedmann, Martin

To develop improved automated subtyping approaches for Listeria monocytogenes, we characterized the discriminatory power of different restriction enzymes for ribotyping. When 15 different restriction...

Molecular Studies on the Ecology of Listeria monocytogenes in the Smoked Fish Processing Industry

Norton, Dawn M., McCamey, Meghan A., Gall, Kenneth L., Scarlett, Janet M., Boor, Kathryn J., Wiedmann, Martin

We have applied molecular approaches, including PCR-based detection strategies and DNA fingerprinting methods, to study the ecology of Listeria monocytogenes in food processing environments. A total...

Characterization and Pathogenic Potential of Listeria monocytogenes Isolates from the Smoked Fish Industry

Norton, Dawn M., Scarlett, Janet M., Horton, Kelly, Sue, David, Thimothe, Joanne, Boor, Kathryn J., ...

This study was designed to evaluate the hypothesis that some of the Listeria monocytogenes subtypes associated with foods, specifically smoked fish, may have an attenuated ability to cause human...

Molecular and Phenotypic Characterization of Pseudomonas spp. Isolated from Milk

Wiedmann, Martin, Weilmeier, Denise, Dineen, Sean S., Ralyea, Robert, Boor, Kathryn J.

Putative Pseudomonas spp. isolated predominantly from raw and processed milk were characterized by automated ribotyping and by biochemical reactions. Isolates were biochemically profiled using the...

General Stress Transcription Factor ςB and Its Role in Acid Tolerance and Virulence of Listeria monocytogenes

Wiedmann, Martin, Arvik, Torey J., Hurley, Richard J., Boor, Kathryn J.

The gene encoding the general stress transcription factor ςB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification....

The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain

Hundley, Heather, Eisenman, Helene, Walter, William, Evans, Tara, Hotokezaka, Yuka, Wiedmann, Martin, ...

Two proteins of the Hsp70 class (Ssb and Ssz1) and one of the J-type class (Zuo1) of molecular chaperones reside on the yeast ribosome, with Ssz1 forming a stable heterodimer with Zuo1. We designed...

Sigma B Contributes to PrfA-Mediated Virulence in Listeria monocytogenes

Nadon, Celine A., Bowen, Barbara M., Wiedmann, Martin, Boor, Kathryn J.

Transcription of the Listeria monocytogenes positive regulatory factor A protein (PrfA) is initiated from either of two promoters immediately upstream of prfA (prfAp1 and prfAp2) or from the upstream...

Rational Design of DNA Sequence-Based Strategies for Subtyping Listeria monocytogenes

Cai, Steven, Kabuki, Dirce Yorika, Kuaye, Arnaldo Yoshiteru, Cargioli, Theresa Gina, Chung, Michael S., Nielsen, Rasmus, ...

The ability to differentiate bacteria beyond the species level is essential for identifying and tracking infectious disease outbreaks and to improve our knowledge of the population genetics,...

Role of σB in Regulating the Compatible Solute Uptake Systems of Listeria monocytogenes: Osmotic Induction of opuC Is σB Dependent

Fraser, Katy R., Sue, David, Wiedmann, Martin, Boor, Kathryn, O'Byrne, Conor P.

The regulation of the compatible solute transport systems in Listeria monocytogenes by the stress-inducible sigma factor σB was investigated. Using wild-type strain 10403S and an otherwise isogenic...

Genome Diversification in Phylogenetic Lineages I and II of Listeria monocytogenes: Identification of Segments Unique to Lineage II Populations†

Zhang, Chaomei, Zhang, Min, Ju, Jingliang, Nietfeldt, Joseph, Wise, John, Terry, Philip M., ...

Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all...

Listeria monocytogenes σB Regulates Stress Response and Virulence Functions

Kazmierczak, Mark J., Mithoe, Sharon C., Boor, Kathryn J., Wiedmann, Martin

While the stress-responsive alternative sigma factor σB has been identified in different species of Bacillus, Listeria, and Staphylococcus, the σB regulon has been extensively characterized only in...

Multilocus Sequence Typing of Listeria monocytogenes by Use of Hypervariable Genes Reveals Clonal and Recombination Histories of Three Lineages

Meinersmann, Richard J., Phillips, Robert W., Wiedmann, Martin, Berrang, Mark E.

In an attempt to develop a method to discriminate among isolates of Listeria monocytogenes, the sequences of all of the annotated genes from the fully sequenced strain L. monocytogenes EGD-e...

Evaluation of “Helicobacter heilmannii” Subtypes in the Gastric Mucosas of Cats and Dogs

Priestnall, Simon L., Wiinberg, Bo, Spohr, Anette, Neuhaus, Britta, Kuffer, Manuela, Wiedmann, Martin, ...

Infection with candidatus “Helicobacter heilmannii” is associated with gastritis and mucosa-associated lymphoid tissue lymphoma in people. Infection with “H. heilmannii” type 1 predominates...

Listeria monocytogenes Isolates from Foods and Humans Form Distinct but Overlapping Populations

Gray, Michael J., Zadoks, Ruth N., Fortes, Esther D., Dogan, Belgin, Cai, Steven, Chen, Yuhuan, ...

A total of 502 Listeria monocytogenes isolates from food and 492 from humans were subtyped by EcoRI ribotyping and PCR-restriction fragment length polymorphism analysis of the virulence gene hly....

Protein translocation across wheat germ microsomal membranes requires an SRP-like component

Prehn, Siegfried, Wiedmann, Martin, Rapoport, Tom A., Zwieb, Christian

Different wheat germ extracts were tested for the presence of membranes capable of translocating and processing nascent secretory proteins. One lysate was found in which nascent prehuman-placental...

Molecular Subtyping and Characterization of Bovine and Human Streptococcus agalactiae Isolates

Sukhnanand, Sharinne, Dogan, Belgin, Ayodele, Maranatha O., Zadoks, Ruth N., Craver, Mary Patricia J., Dumas, Nellie B., ...

Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York...

Multilocus Sequence Typing of Streptococcus uberis Provides Sensitive and Epidemiologically Relevant Subtype Information and Reveals Positive Selection in the Virulence Gene pauA

Zadoks, Ruth N., Schukken, Ynte H., Wiedmann, Martin

Control of the bovine mastitis pathogen Streptococcus uberis requires sensitive and epidemiologically meaningful subtyping methods that can provide insight into this pathogen's epidemiology and...

DNA Sequence-Based Subtyping and Evolutionary Analysis of Selected Salmonella enterica Serotypes

Sukhnanand, Sharinne, Alcaine, Sam, Warnick, Lorin D., Su, Wan-Lin, Hof, Jessica, Craver, Mary Pat J., ...

While serotyping and phage typing have been used widely to characterize Salmonella isolates, sensitive subtyping methods that allow for evolutionary analyses are essential for examining Salmonella...

Alternative Sigma Factors and Their Roles in Bacterial Virulence

Kazmierczak, Mark J., Wiedmann, Martin, Boor, Kathryn J.

Sigma factors provide promoter recognition specificity to RNA polymerase holoenzyme, contribute to DNA strand separation, and then dissociate from the core enzyme following transcription initiation....

Polypeptide-binding proteins mediate completion of co-translational protein translocation into the mammalian endoplasmic reticulum

Tyedmers, Jens, Lerner, Monika, Wiedmann, Martin, Volkmer, Jörg, Zimmermann, Richard

The first step in the secretion of most mammalian proteins is their transport into the lumen of the endoplasmic reticulum (ER). Transport of pre-secretory proteins into the mammalian ER requires...

Definition of Genetically Distinct Attenuation Mechanisms in Naturally Virulence-Attenuated Listeria monocytogenes by Comparative Cell Culture and Molecular Characterization

Roberts, Angela, Chan, Yvonne, Wiedmann, Martin

Listeria monocytogenes is a foodborne pathogen able to cause serious disease in humans and animals. Not all isolates are equally pathogenic, however, and several isolates have been characterized as...

Exposure to Salt and Organic Acids Increases the Ability of Listeria monocytogenes To Invade Caco-2 Cells but Decreases Its Ability To Survive Gastric Stress†

Garner, Matthew R., James, Karen E., Callahan, Michelle C., Wiedmann, Martin, Boor, Kathryn J.

The effects of environmental stress exposure on Listeria monocytogenes growth and virulence-associated characteristics were investigated. Specifically, we measured the effects of temperature (7 or...

Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase

Ryazanov, Alexey G., Ward, Michael D., Mendola, Charmaine E., Pavur, Karen S., Dorovkov, Maxim V., Wiedmann, Martin, ...

The several hundred members of the eukaryotic protein kinase superfamily characterized to date share a similar catalytic domain structure, consisting of 12 conserved subdomains. Here we report the...

A general mechanism for regulation of access to the translocon: Competition for a membrane attachment site on ribosomes

Möller, Ines, Jung, Martin, Beatrix, Birgitta, Levy, Robert, Kreibich, Gert, Zimmermann, Richard, ...

For proteins to enter the secretory pathway, the membrane attachment site (M-site) on ribosomes must bind cotranslationally to the Sec61 complex present in the endoplasmic reticulum membrane. The...

Automated Ribotyping Using Different Enzymes To Improve Discrimination of Listeria monocytogenes Isolates, with a Particular Focus on Serotype 4b Strains

De Cesare, Alessandra, Bruce, James L., Dambaugh, Timothy R., Guerzoni, Maria E., Wiedmann, Martin

To develop improved automated subtyping approaches for Listeria monocytogenes, we characterized the discriminatory power of different restriction enzymes for ribotyping. When 15 different restriction...

Molecular Studies on the Ecology of Listeria monocytogenes in the Smoked Fish Processing Industry

Norton, Dawn M., McCamey, Meghan A., Gall, Kenneth L., Scarlett, Janet M., Boor, Kathryn J., Wiedmann, Martin

We have applied molecular approaches, including PCR-based detection strategies and DNA fingerprinting methods, to study the ecology of Listeria monocytogenes in food processing environments. A total...

Characterization and Pathogenic Potential of Listeria monocytogenes Isolates from the Smoked Fish Industry

Norton, Dawn M., Scarlett, Janet M., Horton, Kelly, Sue, David, Thimothe, Joanne, Boor, Kathryn J., ...

This study was designed to evaluate the hypothesis that some of the Listeria monocytogenes subtypes associated with foods, specifically smoked fish, may have an attenuated ability to cause human...

Molecular and Phenotypic Characterization of Pseudomonas spp. Isolated from Milk

Wiedmann, Martin, Weilmeier, Denise, Dineen, Sean S., Ralyea, Robert, Boor, Kathryn J.

Putative Pseudomonas spp. isolated predominantly from raw and processed milk were characterized by automated ribotyping and by biochemical reactions. Isolates were biochemically profiled using the...

General Stress Transcription Factor ςB and Its Role in Acid Tolerance and Virulence of Listeria monocytogenes

Wiedmann, Martin, Arvik, Torey J., Hurley, Richard J., Boor, Kathryn J.

The gene encoding the general stress transcription factor ςB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification....

The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain

Hundley, Heather, Eisenman, Helene, Walter, William, Evans, Tara, Hotokezaka, Yuka, Wiedmann, Martin, ...

Two proteins of the Hsp70 class (Ssb and Ssz1) and one of the J-type class (Zuo1) of molecular chaperones reside on the yeast ribosome, with Ssz1 forming a stable heterodimer with Zuo1. We designed...

Sigma B Contributes to PrfA-Mediated Virulence in Listeria monocytogenes

Nadon, Celine A., Bowen, Barbara M., Wiedmann, Martin, Boor, Kathryn J.

Transcription of the Listeria monocytogenes positive regulatory factor A protein (PrfA) is initiated from either of two promoters immediately upstream of prfA (prfAp1 and prfAp2) or from the upstream...

Rational Design of DNA Sequence-Based Strategies for Subtyping Listeria monocytogenes

Cai, Steven, Kabuki, Dirce Yorika, Kuaye, Arnaldo Yoshiteru, Cargioli, Theresa Gina, Chung, Michael S., Nielsen, Rasmus, ...

The ability to differentiate bacteria beyond the species level is essential for identifying and tracking infectious disease outbreaks and to improve our knowledge of the population genetics,...

Role of σB in Regulating the Compatible Solute Uptake Systems of Listeria monocytogenes: Osmotic Induction of opuC Is σB Dependent

Fraser, Katy R., Sue, David, Wiedmann, Martin, Boor, Kathryn, O'Byrne, Conor P.

The regulation of the compatible solute transport systems in Listeria monocytogenes by the stress-inducible sigma factor σB was investigated. Using wild-type strain 10403S and an otherwise isogenic...

Genome Diversification in Phylogenetic Lineages I and II of Listeria monocytogenes: Identification of Segments Unique to Lineage II Populations†

Zhang, Chaomei, Zhang, Min, Ju, Jingliang, Nietfeldt, Joseph, Wise, John, Terry, Philip M., ...

Thirteen different serotypes of Listeria monocytogenes can be distinguished on the basis of variation in somatic and flagellar antigens. Although the known virulence genes are present in all...

Listeria monocytogenes σB Regulates Stress Response and Virulence Functions

Kazmierczak, Mark J., Mithoe, Sharon C., Boor, Kathryn J., Wiedmann, Martin

While the stress-responsive alternative sigma factor σB has been identified in different species of Bacillus, Listeria, and Staphylococcus, the σB regulon has been extensively characterized only in...

Multilocus Sequence Typing of Listeria monocytogenes by Use of Hypervariable Genes Reveals Clonal and Recombination Histories of Three Lineages

Meinersmann, Richard J., Phillips, Robert W., Wiedmann, Martin, Berrang, Mark E.

In an attempt to develop a method to discriminate among isolates of Listeria monocytogenes, the sequences of all of the annotated genes from the fully sequenced strain L. monocytogenes EGD-e...

Evaluation of “Helicobacter heilmannii” Subtypes in the Gastric Mucosas of Cats and Dogs

Priestnall, Simon L., Wiinberg, Bo, Spohr, Anette, Neuhaus, Britta, Kuffer, Manuela, Wiedmann, Martin, ...

Infection with candidatus “Helicobacter heilmannii” is associated with gastritis and mucosa-associated lymphoid tissue lymphoma in people. Infection with “H. heilmannii” type 1 predominates...

Listeria monocytogenes Isolates from Foods and Humans Form Distinct but Overlapping Populations

Gray, Michael J., Zadoks, Ruth N., Fortes, Esther D., Dogan, Belgin, Cai, Steven, Chen, Yuhuan, ...

A total of 502 Listeria monocytogenes isolates from food and 492 from humans were subtyped by EcoRI ribotyping and PCR-restriction fragment length polymorphism analysis of the virulence gene hly....

Protein translocation across wheat germ microsomal membranes requires an SRP-like component

Prehn, Siegfried, Wiedmann, Martin, Rapoport, Tom A., Zwieb, Christian

Different wheat germ extracts were tested for the presence of membranes capable of translocating and processing nascent secretory proteins. One lysate was found in which nascent prehuman-placental...

Molecular Subtyping and Characterization of Bovine and Human Streptococcus agalactiae Isolates

Sukhnanand, Sharinne, Dogan, Belgin, Ayodele, Maranatha O., Zadoks, Ruth N., Craver, Mary Patricia J., Dumas, Nellie B., ...

Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York...

Multilocus Sequence Typing of Streptococcus uberis Provides Sensitive and Epidemiologically Relevant Subtype Information and Reveals Positive Selection in the Virulence Gene pauA

Zadoks, Ruth N., Schukken, Ynte H., Wiedmann, Martin

Control of the bovine mastitis pathogen Streptococcus uberis requires sensitive and epidemiologically meaningful subtyping methods that can provide insight into this pathogen's epidemiology and...

Definition of Genetically Distinct Attenuation Mechanisms in Naturally Virulence-Attenuated Listeria monocytogenes by Comparative Cell Culture and Molecular Characterization

Roberts, Angela, Chan, Yvonne, Wiedmann, Martin

Listeria monocytogenes is a foodborne pathogen able to cause serious disease in humans and animals. Not all isolates are equally pathogenic, however, and several isolates have been characterized as...

DNA Sequence-Based Subtyping and Evolutionary Analysis of Selected Salmonella enterica Serotypes

Sukhnanand, Sharinne, Alcaine, Sam, Warnick, Lorin D., Su, Wan-Lin, Hof, Jessica, Craver, Mary Pat J., ...

While serotyping and phage typing have been used widely to characterize Salmonella isolates, sensitive subtyping methods that allow for evolutionary analyses are essential for examining Salmonella...

Alternative Sigma Factors and Their Roles in Bacterial Virulence

Kazmierczak, Mark J., Wiedmann, Martin, Boor, Kathryn J.

Sigma factors provide promoter recognition specificity to RNA polymerase holoenzyme, contribute to DNA strand separation, and then dissociate from the core enzyme following transcription initiation....

Polypeptide-binding proteins mediate completion of co-translational protein translocation into the mammalian endoplasmic reticulum

Tyedmers, Jens, Lerner, Monika, Wiedmann, Martin, Volkmer, Jörg, Zimmermann, Richard

The first step in the secretion of most mammalian proteins is their transport into the lumen of the endoplasmic reticulum (ER). Transport of pre-secretory proteins into the mammalian ER requires...

Exposure to Salt and Organic Acids Increases the Ability of Listeria monocytogenes To Invade Caco-2 Cells but Decreases Its Ability To Survive Gastric Stress†

Garner, Matthew R., James, Karen E., Callahan, Michelle C., Wiedmann, Martin, Boor, Kathryn J.

The effects of environmental stress exposure on Listeria monocytogenes growth and virulence-associated characteristics were investigated. Specifically, we measured the effects of temperature (7 or...

Listeria monocytogenes Subgroups IIIA, IIIB, and IIIC Delineate Genetically Distinct Populations with Varied Pathogenic Potential▿

Liu, Dongyou, Lawrence, Mark L., Wiedmann, Martin, Gorski, Lisa, Mandrell, Robert E., Ainsworth, A. Jerald, ...

Listeria monocytogenes lineage III strains belonging to subgroups IIIA (n = 8), IIIB (n = 5), and IIIC (n = 6) were examined along with other known serotype strains (n = 11) by PCR and Southern...

The Alternative Sigma Factor σB and the Virulence Gene Regulator PrfA Both Regulate Transcription of Listeria monocytogenes Internalins▿ †

McGann, Patrick, Wiedmann, Martin, Boor, Kathryn J.

Some Listeria monocytogenes internalins are recognized as contributing to invasion of mammalian tissue culture cells. While PrfA is well established as a positive regulator of L. monocytogenes...

Temperature-Dependent Expression of Listeria monocytogenes Internalin and Internalin-Like Genes Suggests Functional Diversity of These Proteins among the Listeriae▿ †

McGann, Patrick, Ivanek, Renata, Wiedmann, Martin, Boor, Kathryn J.

The Listeria monocytogenes genome contains genes encoding several internalins and internalin-like proteins. As L. monocytogenes is present in many environments and can infect numerous, diverse host...

σB-Dependent and σB-Independent Mechanisms Contribute to Transcription of Listeria monocytogenes Cold Stress Genes during Cold Shock and Cold Growth▿ †

Chan, Yvonne C., Boor, Kathryn J., Wiedmann, Martin

The role of the stress response regulator σB (encoded by sigB) in directing the expression of selected putative and confirmed cold response genes was evaluated using Listeria monocytogenes 10403S...

Microarray-Based Characterization of the Listeria monocytogenes Cold Regulon in Log- and Stationary-Phase Cells▿ †

Chan, Yvonne C., Raengpradub, Sarita, Boor, Kathryn J., Wiedmann, Martin

Whole-genome microarray experiments were performed to define the Listeria monocytogenes cold growth regulon and to identify genes differentially expressed during growth at 4 and 37°C. Microarray...

Phenotypic and Transcriptomic Analyses Demonstrate Interactions between the Transcriptional Regulators CtsR and Sigma B in Listeria monocytogenes▿ †

Hu, Yuewei, Raengpradub, Sarita, Schwab, Ute, Loss, Chris, Orsi, Renato H., Wiedmann, Martin, ...

Listeria monocytogenes σB positively regulates the transcription of class II stress response genes; CtsR negatively regulates class III stress response genes. To identify interactions between these...

Transcriptomic and Phenotypic Analyses Suggest a Network between the Transcriptional Regulators HrcA and σB in Listeria monocytogenes▿ †

Hu, Yuewei, Oliver, Haley F., Raengpradub, Sarita, Palmer, M. Elizabeth, Orsi, Renato H., Wiedmann, Martin, ...

Listeria monocytogenes HrcA and CtsR negatively regulate class I and III stress response genes, respectively, while σB positively regulates the transcription of class II stress response genes. To...

Comparative Analysis of the σB-Dependent Stress Responses in Listeria monocytogenes and Listeria innocua Strains Exposed to Selected Stress Conditions▿ †

Raengpradub, Sarita, Wiedmann, Martin, Boor, Kathryn J.

The alternative sigma factor σB contributes to transcription of stress response and virulence genes in diverse gram-positive bacterial species. The composition and functions of the Listeria...

Listeria monocytogenes σB Modulates PrfA-Mediated Virulence Factor Expression▿ †

Ollinger, Juliane, Bowen, Barbara, Wiedmann, Martin, Boor, Kathryn J., Bergholz, Teresa M.

Listeria monocytogenes σB and positive regulatory factor A (PrfA) are pleiotropic transcriptional regulators that coregulate a subset of virulence genes. A positive regulatory role for σB in prfA...