Nakamura, Ayako, Fujioka, Shozo, Takatsuto, Suguru, Tsujimoto, Masafumi, Kitano, Hidemi, Yoshida, Shigeo, ...
The rice lamina joint is ideal material for investigating the activity of brassinosteroids (BRs) and auxin because of its high sensitivity to these compounds. Using a series of rice BR biosynthetic...
Akiyama, Nobuko, Nakanishi, Ikuo, Ohkubo, Kei, Satoh, Kazue, Tsuchiya, Koichiro, Nishikawa, Takeshi, ...
N-β-Alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), an insect-derived antibacterial peptide, generates hydrogen peroxide (H2O2) that exerts antitumour activity. We have investigated...
Ligand-binding Activity and Expression Profile of Annexins in Caenorhabditis elegans (2007)
Nishioka, Sara, Aikawa, Jun-ichi, Ida, Michiru, Matsumoto, Isamu, Street, Miyoko, Tsujimoto, Masafumi, ...
Mammalian annexins are implicated in several physiological mechanisms based on their calcium-dependent phospholipid/membrane binding and carbohydrate-binding activities. In this study, we...
Funakoshi, Yuji, Doi, Yusuke, Hosoda, Nao, Uchida, Naoyuki, Osawa, Masanori, Shimada, Ichio, ...
In eukaryotes, shortening of the 3′-poly(A) tail is the rate-limiting step in the degradation of most mRNAs, and two major mRNA deadenylase complexes—Caf1–Ccr4 and Pan2–Pan3—play central...
Ligand-binding activity and expression profile of annexins in Caenorhabditis elegans (2006)
Nishioka, Sara, Aikawa, Jun-ichi, Ida, Michiru, Matsumoto, Isamu, Street, Miyoko, Tsujimoto, Masafumi, ...
Mamalian annexins are implicated in several physiological mechanisms based on their calcium-dependent phospholipid/membrane binding and carbohydrate-binding activities. In this study, we investigated...
Takashima, Shou, Tsuji, Shuichi, Tsujimoto, Masafumi
The cDNA encoding a second type of mouse &bgr;-galactoside α2,6-sialyltransferase (ST6Gal II) was cloned and characterized. The sequence of mouse ST6Gal II encoded a protein of 524 amino acids and...
Ikoma, Yoko, Nomura, Seiji, Ito, Tomomi, Katsumata, Yoshinari, Nakata, Masayuki, Iwanaga, Kumi, ...
In addition to prostaglandins, inflammatory cytokines induce uterine contraction via oxytocin (OT). Placental leucine aminopeptidase (P‐LAP), an oxytocinase that is identical to cystine...
Aoki, Kazuma, Ishii, Yasuhiro, Matsumoto, Ken, Tsujimoto, Masafumi
Cold‐inducible RNA‐binding protein (CIRP) was originally found in mammalian cells as a protein that is overexpressed upon a temperature downshift. Recently, we identified a Xenopus homolog...
Hattori, Akira, Matsumoto, Ken, Mizutani, Shigehiko, Tsujimoto, Masafumi
The genomic organization of the gene encoding the human adipocyte-derived leucine aminopeptidase (A-LAP) has been determined. The gene is composed of 20 exons and 19 introns and spans approximately...
CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes (2000)
Matsumoto, Ken, Aoki, Kazuma, Dohmae, Naoshi, Takio, Koji, Tsujimoto, Masafumi
In an attempt to isolate mRNA-binding proteins we fractionated Xenopus oocyte lysate by oligo(dT)–cellulose chromatography. A 20 kDa protein was the major component of the eluate. cDNA cloning...
Hattori, Akira, Matsumoto, Hideko, Mizutani, Shigehiko, Tsujimoto, Masafumi
In the current study, we report the cloning and initial characterization of a novel human cytosolic aminopeptidase named adipocyte-derived leucine aminopeptidase (A-LAP). The sequence encodes a...
Ishida, Nobuhiro, Tsujimoto, Masafumi, Kanaya, Toshimichi, Shimamura, Akio, Tsuruoka, Nobuo, Kodama, Shiho, ...
Recombinant human bone morphogenetic protein-2 (rhBMP-2) was expressed in silkworm larvae, and a milligram quantity of the protein was purified and characterized. The expressed rhBMP-2 was...
Characterization of recombinant murine interleukin 5 expressed in Chinese hamster ovary cells (1992)
Kodama, Shiho, Endo, Tamao, Tsujimoto, Masafumi, Kobata, Akira
We have purified recombinant murine mterleukin 5 (rmIL-5) from the supernatant of Chinese hamster ovary cells. Each peptide fragment of the purified rmIL-5 generated by Achromobacter protease I...
Carbohydrate Structures of Human Interleukin 5 Expressed in Chinese Hamster Ovary Cells (1991)
Kodama, Shiho, Endo, Tamao, Tsuruoka, Nobuo, Tsujimoto, Masafumi, Kobata, Akira
The asparagine-linked sugar chains of recombinant human interleukin 5 produced by Chinese hamster ovary cells were released quantitatively as oligosaccharides by hydra-zinolysis. After N-acetylation...
Structure of Recombinant Human Interleukin 5 Produced by Chinese Hamster Ovary Cells (1990)
Minamitake, Yoshiharu, Kodama, Shiho, Katayama, Toyoko, Adachi, Hideki, Tanaka, Shoji, Tsujimoto, Masafumi
The complete peptide map of purified recombinant human interleukin 5 (rhIL-5) was determined to verify its primary structure, glycosylation sites, and disulfide bonding structure. Each peptide...
Purification and Characterization of Human Microsomal Dipeptidase (1989)
Adachi, Hideki, Kubota, Ichiro, Okamura, Nobutaka, Iwata, Hiromitsu, Tsujimoto, Masafumi, Nakazato, Hiroshi, ...
Human microsomal dipeptidase (MDP, formerly referred to as dehydropeptidase-I or renal dipeptidase) [EC 3.4.13.11] was solubilized from the membrane fraction of kidney by treatment with...
Tsujimoto, Masafumi, Adachi, Hideki, Kodama, Shiho, Tsuruoka, Nobuo, Yamada, Yukio, Tanaka, Shoji, ...
Recombinant human interleukin 5 (rhIL-5) expressed in Chinese hamster ovary cells was purified and characterized. Molecular heterogeneity was observed by sodium dodecyl sulfate-polyacrylamide gel...
Effects of Steroid Hormones on the Cytotoxic Activity of Tumor Necrosis Factor (1988)
Tsujimoto, Masafumi, Adachi, Hideki
The effects of steroid hormones on the cytotoxic activity of tumor necrosis factor (TNF) were examined. Dexamethasone and hydrocortisone inhibited the cytotoxic action of TNF toward murine L929...
TSUJIMOTO, Masafumi, TANAKA, Shoji, SAKURAGAWA, Yayoi, TSURUOKA, Nobuo, FUNAKOSHI, Kyoko, BUTSUGAN, Tomoko, ...
Biological activities of human tumor necrosis factor (TNF) and its derivatives were compared. In cytotoxicity assay with L929 cells, one derivative, designated as TNF(Asn), showed significantly lower...
Tumor Necrosis Factor-Induced Downregulation of Its Receptors in HeLa Cells (1987)
TSUJIMOTO, Masafumi, VILcEK, Jan
Tumor necrosis factor (TNF) induced loss of TNF receptors in HeLa cells was studied using acid elution technique, which could distinguish surface occupancy and real loss of receptors. Exposure of...
OKU, Naoto, ARAKI, Ryuichi, ARAKI, Hiroko, SHIBAMOTO, Sayumi, ITO, Fumiaki, NISHIHARA, Tatsuro, ...
The effect of human tumor necrosis factor (TNF) on the permeability properties of liposomal membranes was investigated. TNF caused an increase in permeability of liposomes containing...
Expression in Escherichia of chemically synthesized gene for the human immune Interferon (1983)
Tanaka, Shoji, Oshima, Takehiro, Ohsuye, Kazuhiro, Ono, Teiichi, Mizono, Akira, Ueno, Atsuko, ...
A 454 base pair fragment of double stranded DNA consisting of a gene for a human Immune interferon (hIFN-γ), initiation and termination signals plus appropriate restriction endonuclease sites, was...
Purification and Characterization of Human Serum C-Reactive Protein (1983)
TSUJIMOTO, Masafumi, INOUE, Keizo, NOJIMA, Shoshichi
A simple and rapid purification method for human serum C-reactive protein (CRP) was developed. CRP was strongly adsorbed on a DEAE-cellulose column and was easily separated from other serum proteins....
Reactivity of Human C-Reactive Protein with Positively Charged Liposomes (1981)
TSUJIMOTO, Masafumi, INOUE, Keizo, NOJIMA, Shoshichi
Interaction of CRP with liposomes was studied by agglutination, complement consumption, and the binding assay. Liposomes composed of phosphatidyicholine and stearylamine were agglutinated with CRP....
TSUJIMOTO, Masafumi, INOUE, Keizo, NOJIMA, Shoshichi
CRP-induced agglutination of lipid suspensions prepared in the presence and absence of phosphatidylcholine was studied by a newly devised quantitative method. CRP caused as much agglutination of...
Ikemoto, Mamoru, Arai, Hiroyuki, Feng, Dongdong, Tanaka, Kazumi, Aoki, Junken, Dohmae, Naoshi, ...
The scavenger receptor class B type I (SR-BI) mediates the selective uptake of cholesteryl esters from high-density lipoprotein (HDL) and cholesterol secretion into bile in the liver. In this study,...
Matsumoto, Ken, Nagata, Kyosuke, Miyaji-Yamaguchi, Mary, Kikuchi, Akihiko, Tsujimoto, Masafumi
Template activating factor I (TAF-I) was originally identified as a host factor required for DNA replication and transcription of adenovirus genome complexed with viral basic proteins. Purified TAF-I...
CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes
Matsumoto, Ken, Aoki, Kazuma, Dohmae, Naoshi, Takio, Koji, Tsujimoto, Masafumi
In an attempt to isolate mRNA-binding proteins we fractionated Xenopus oocyte lysate by oligo(dT)–cellulose chromatography. A 20 kDa protein was the major component of the eluate. cDNA cloning...
Okuwaki, Mitsuru, Tsujimoto, Masafumi, Nagata, Kyosuke
Nucleophosmin/B23 is a nucleolar phosphoprotein. It has been shown that B23 binds to nucleic acids, digests RNA, and is localized in nucleolar granular components from which preribosomal particles...
Aoki, Kazuma, Ishii, Yasuhiro, Matsumoto, Ken, Tsujimoto, Masafumi
Cold-inducible RNA-binding protein (CIRP) was originally found in mammalian cells as a protein that is overexpressed upon a temperature downshift. Recently, we identified a Xenopus homolog of CIRP,...
Inoue, Takao, Sugimoto, Asako, Suzuki, Yuka, Yamamoto, Masayuki, Tsujimoto, Masafumi, Inoue, Keizo, ...
Type II platelet-activating factor-acetylhydrolase [PAF-AH (II)] is an N-myristoylated enzyme that contains a lipase/esterase catalytic motif and selectively hydrolyzes the sn-2 acetyl ester of PAF...
Matsumoto, Ken, Tanaka, Kimio J., Tsujimoto, Masafumi
Eukaryotic Y-box proteins are nucleic acid-binding proteins implicated in a wide range of gene regulatory mechanisms. They contain the cold shock domain, which is a nucleic acid-binding structure...
Regulation of cytosolic prostaglandin E synthase by phosphorylation
Kobayashi, Tsuyoshi, Nakatani, Yoshihito, Tanioka, Toshihiro, Tsujimoto, Masafumi, Nakajo, Shigeo, Nakaya, Kazuyasu, ...
cPGES [cytosolic PG (prostaglandin) E synthase] is constitutively expressed in various cells and can regulate COX (cyclo-oxygenase)-1-dependent immediate PGE2 generation. In the present study, we...
Regulation of SR-BI protein levels by phosphorylation of its associated protein, PDZK1
Nakamura, Toshiyuki, Shibata, Norihito, Nishimoto-Shibata, Takako, Feng, Dongdong, Ikemoto, Mamoru, Motojima, Kiyoto, ...
Scavenger receptor class B type I (SR-BI) is a high-density lipoprotein (HDL) receptor that mediates the selective uptake of HDL cholesterol and cholesterol secretion into bile in the liver....
Ikemoto, Mamoru, Arai, Hiroyuki, Feng, Dongdong, Tanaka, Kazumi, Aoki, Junken, Dohmae, Naoshi, ...
The scavenger receptor class B type I (SR-BI) mediates the selective uptake of cholesteryl esters from high-density lipoprotein (HDL) and cholesterol secretion into bile in the liver. In this study,...
Matsumoto, Ken, Nagata, Kyosuke, Miyaji-Yamaguchi, Mary, Kikuchi, Akihiko, Tsujimoto, Masafumi
Template activating factor I (TAF-I) was originally identified as a host factor required for DNA replication and transcription of adenovirus genome complexed with viral basic proteins. Purified TAF-I...
CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes
Matsumoto, Ken, Aoki, Kazuma, Dohmae, Naoshi, Takio, Koji, Tsujimoto, Masafumi
In an attempt to isolate mRNA-binding proteins we fractionated Xenopus oocyte lysate by oligo(dT)–cellulose chromatography. A 20 kDa protein was the major component of the eluate. cDNA cloning...
Okuwaki, Mitsuru, Tsujimoto, Masafumi, Nagata, Kyosuke
Nucleophosmin/B23 is a nucleolar phosphoprotein. It has been shown that B23 binds to nucleic acids, digests RNA, and is localized in nucleolar granular components from which preribosomal particles...
Aoki, Kazuma, Ishii, Yasuhiro, Matsumoto, Ken, Tsujimoto, Masafumi
Cold-inducible RNA-binding protein (CIRP) was originally found in mammalian cells as a protein that is overexpressed upon a temperature downshift. Recently, we identified a Xenopus homolog of CIRP,...
Inoue, Takao, Sugimoto, Asako, Suzuki, Yuka, Yamamoto, Masayuki, Tsujimoto, Masafumi, Inoue, Keizo, ...
Type II platelet-activating factor-acetylhydrolase [PAF-AH (II)] is an N-myristoylated enzyme that contains a lipase/esterase catalytic motif and selectively hydrolyzes the sn-2 acetyl ester of PAF...
Matsumoto, Ken, Tanaka, Kimio J., Tsujimoto, Masafumi
Eukaryotic Y-box proteins are nucleic acid-binding proteins implicated in a wide range of gene regulatory mechanisms. They contain the cold shock domain, which is a nucleic acid-binding structure...
Regulation of cytosolic prostaglandin E synthase by phosphorylation
Kobayashi, Tsuyoshi, Nakatani, Yoshihito, Tanioka, Toshihiro, Tsujimoto, Masafumi, Nakajo, Shigeo, Nakaya, Kazuyasu, ...
cPGES [cytosolic PG (prostaglandin) E synthase] is constitutively expressed in various cells and can regulate COX (cyclo-oxygenase)-1-dependent immediate PGE2 generation. In the present study, we...
Regulation of SR-BI protein levels by phosphorylation of its associated protein, PDZK1
Nakamura, Toshiyuki, Shibata, Norihito, Nishimoto-Shibata, Takako, Feng, Dongdong, Ikemoto, Mamoru, Motojima, Kiyoto, ...
Scavenger receptor class B type I (SR-BI) is a high-density lipoprotein (HDL) receptor that mediates the selective uptake of HDL cholesterol and cholesterol secretion into bile in the liver....
Funakoshi, Yuji, Doi, Yusuke, Hosoda, Nao, Uchida, Naoyuki, Osawa, Masanori, Shimada, Ichio, ...
In eukaryotes, shortening of the 3′-poly(A) tail is the rate-limiting step in the degradation of most mRNAs, and two major mRNA deadenylase complexes—Caf1–Ccr4 and Pan2–Pan3—play central...
Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP
Kawahara, Hironori, Imai, Takao, Imataka, Hiroaki, Tsujimoto, Masafumi, Matsumoto, Ken, Okano, Hideyuki
Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells. We previously reported that Msi1 contributes to the maintenance of the immature state and self-renewal...
β-Catenin asymmetry is regulated by PLA1 and retrograde traffic in C. elegans stem cell divisions
Kanamori, Takahiro, Inoue, Takao, Sakamoto, Taro, Gengyo-Ando, Keiko, Tsujimoto, Masafumi, Mitani, Shohei, ...
Asymmetric division is an important property of stem cells. In Caenorhabditis elegans, the Wnt/β-catenin asymmetry pathway determines the polarity of most asymmetric divisions. The Wnt signalling...