Masamichi Takagi

Field Array Compression in Data Caches for Dynamically Allocated Recursive Data Structures (2008)

Masamichi Takagi, Kei Hiraki

Abstract. We introduce a software/hardware scheme called the Field Array Compression Technique (FACT) which reduces cache misses caused by recursive data structures. Using a data layout...

Compression in Data Caches with Compressible Field Isolation for Recursive Data Structures (2008)

Masamichi Takagi, Kei Hiraki

Abstract. We introduce a software/hardware scheme called the Field Array Compression Technique (FACT) which reduces cache misses due to recursive data structures. Using a data layout transformation,...

The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity (2004)

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

Transfer RNA (tRNA) 3′ processing endoribonuclease (tRNase Z) is an enzyme responsible for the removal of a 3′ trailer from pre-tRNA. There exists two types of tRNase Z: one is a short form...

A novel 4-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules (2004)

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

Mammalian tRNase ZL shows versatility in substrate recognition. This enzyme can not only process pre-tRNAs by cleaving off their 3′ trailer sequences, but also recognize and cleave pre-tRNA-like...

A candidate prostate cancer susceptibility gene encodes tRNA 3' processing endoribonuclease (2003)

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

tRNA 3′ processing endoribonuclease (3′ tRNase) is an enzyme responsible for the removal of a 3′ trailer from precursor tRNA (pre‐tRNA). We purified ∼85 kDa 3′ tRNase from pig liver...

Employment of the Human Estrogen Receptor {beta} Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters (2002)

Lee, Haeng-Seog, Miyauchi, Keisuke, Nagata, Yuji, Fukuda, Ryouichi, Sasagawa, Shin-ichi, Endoh, Hideki, ...

To screen a wide variety of chemicals for endocrine disrupters, and to develop an effective microbial degradation system for them, a good system is needed for the rapid and accurate evaluation of the...

Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development (2000)

Fujiwara, Makoto, Ichinomiya, Masayuki, Motoyama, Takayuki, Horiuchi, Hiroyuki, Ohta, Akinori, Takagi, Masamichi

Although many chitin synthase genes have been identified in a broad range of fungal species, there have been only a few reports about their role in fungal morphogenesis. In most cases, single gene...

Expression of Plant Group 2 and Group 3 lea Genes in Saccharomyces cerevisiae Revealed Functional Divergence among LEA Proteins (2000)

Zhang, Lei, Ohta, Akinori, Takagi, Masamichi, Imai, Ryozo

To study functions of late embryogenesis abundant (LEA) proteins, which accumulate in plant cells under water deficit conditions, in vivo functional analyses were carried out using a yeast...

Misfolded Membrane-Bound Cytochrome P450 Activates KAR2 Induction through Two Distinct Mechanisms (1999)

Zimmer, Thomas, Ogura, Atsushi, Ohta, Akinori, Takagi, Masamichi

Using the mRNA differential display technique and Western blot analysis, the present study demonstrates that induction of KAR2 occurs when misfolded membrane-bound cytochrome P450, mutated in its...

Isolation and Characterization of ECTl Gene Encoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae (1996)

Min-Seek, Rho, Kawamata, Yuji, Nakamura, Hidemitsu, Ohta, Akinori, Takagi, Masamichi

Sacchammyces cerevisiae mutants that were unable to utilize extracellular ethanolamine for phosphatidylethanolamine synthesis were isolated. Two of them carried recessive chromosomal mutations in a...

The 5' Flanking Sequences of Two S Alleles in Lycopersicon peruvianum Are Highly Heterologous but Contain Short Blocks of Homologous Sequences (1995)

Chung, Kyung, Lee, Sang Yeb, Ito, Toru, Tanaka, Hiroshi, Nam, Hong Gil, Takagi, Masamichi

To characterize the molecular structure of the S alleles in Lycopersicon peruvianum, we isolated two genomic fragments that corresponded to cDNAs for S11 and S12 RNases from a genomic library of a...

pH Profile of Kinetic Constants of RNase Rh from Rhizopus niveus and Its Mutant Enzymes towards UpU, and Possible Mechanisms of RNase Rh (1994)

Irie, Masachika, Ohgi, Kazuko, Watanabe, Hideaki, Iwama, Masanori, Nakamura, Kazuo T., Kurihara, Hiroyuki, ...

In order to elucidate the mechanism of action of Rhizopus niveus RNase Rh, we investigated the pH profiles of the kinetic parameters of RNase RNAP Rh, a derivative of RNase Rh, and its mutant...

Role of Asp51 and GlulO5 in the Enzymatic Activity of a Ribonuclease from Rhizopus niveus (1993)

Ohgi, Kazuko, Horiuchi, Hiroyuki, Watanabe, Hideaki, Iwama, Masanori, Takagi, Masamichi, Irie, Masachika

The active site of a base non-specific RNase from Rhizopus niveus (RNase Rh), consists of three histidine residues and one carboxyl group [Ohgi, K. et al. (1992) J. Biochem. 111, 132–138]. In order...

Evidence That Three Histidine Residues of a Base Non-Specific and Adenylic Acid Preferential Ribonuclease from Rhizopus niveus Are Involved in the Catalytic Function (1992)

Ohgi, Kazuko, Horiuchi, Hiroyuki, Watanabe, Hideaki, Iwama, Masanori, Takagi, Masamichi, Irie, Masachika

In order to study the structure-function relationship of an RNase T2 family enzyme, RNase Rh, from Rhizopus niveus, we investigated the roles of three histidine residues by means of site-specific...

Expression of RNase Rh from Rhizopus niveus in Yeast and Characterization of the Secreted Proteins (1991)

Ohgi, Kazuko, Horiuchi, Hiroyuki, Watanabe, Hideaki, Takagi, Masamichi, Yano, Keiji, Irie, Masachika

The full-length cDNA encoding RNase Rh, which is secreted extracellularly by Rhizopus niveus, was isolated and its nucleotide sequence was determined. It was placed under control of the promoter of...

Primary Structure of a Base Non-Specific Ribonuclease from Rhizopus niveus (1988)

Horiuchi, Hiroyuki, Yanai, Koji, Takagi, Masamichi, Yano, Keiji, Wakabayashi, Eiji, Sanda, Akihiro, ...

The primary structure of a base non-specific ribonuclease from Rhizopus niveus (RNase Rh) was determined by nucleotide sequence analysis of the DNA fragment encoding RNase Rh gene including signal...

A new restriction endonudease from Spirulina platensis (1986)

Kawamura, Masahide, Sakakibara, Masaki, Watanabe, Teruo, Kita, Keiko, Hiraoka, Nobutsugu, Obayashi, Akira, ...

Three restriction endonucleases, SplI, SplII and SplIII have been purified partially from Spirulina platensis subspecies sjampse and named. SplI cleaves bacteriophage lambda DNA at one site, phi X...

Stimulatory Effect of n-Alkanes on CTP: Cholinephosphate Cytidyltransferase Activity in Rat Liver Microsomal Membranes In Vitro (1977)

TAKAGI, Masamichi

To assess the effect of alteration of membrane structure on the enzymic activities related to phospholipid synthesis in microsomal membrane, the effects of several organic solvents have been studied...

Binding of Polysomes In Vitro with Endoplasmic Membrane Prepared from Rat Liver: II. Possible Involvement of Cytochrome P-450 in the Binding (1977)

TAKAGI, Masamichi

Using an in vitro polysome-membrane binding system from rat liver developed by us, the effect of structural alteration of the microsomal membrane on its polysome-binding capacity was examined....

A Polysome-Membrane Binding System from Rat Liver: I. Basic Characterization of the Binding System (1975)

TAKAGI, Masamichi, HOAGLAND, Mahlon B.

A simple reaction system was developed to examine the binding of polysomes to membranes of the endoplasmic reticulum and to investigate the fate of ribosomes and nascent chains during protein...

Polysomal RNA's of Rat Liver: Identification and Characterization of Several Kinds of High Molecular Weight RNA Other than 18S and 28S Species (1971)

TAKAGI, Masamichi, INOUE, Tadashi, UMEMURA, Yasukiyo

RNA was extracted from polysomes of rat liver by SDS-phenol method in the presence of bentonite and analyzed by polyacrylamide gel electrophoresis. When the gel concentration of 3.1% was used,...

Proliferation of Intrahyphal Hyphae Caused by Disruption of csmA, Which Encodes a Class V Chitin Synthase with a Myosin Motor-Like Domain in Aspergillus nidulans

Horiuchi, Hiroyuki, Fujiwara, Makoto, Yamashita, Shuichi, Ohta, Akinori, Takagi, Masamichi

We have found that the Aspergillus nidulans csmA gene encodes a novel protein which consists of an N-terminal myosin motor-like domain and a C-terminal chitin synthase domain (M. Fujiwara, H....

Two Different Types of Dehalogenases, LinA and LinB, Involved in γ-Hexachlorocyclohexane Degradation in Sphingomonas paucimobilis UT26 Are Localized in the Periplasmic Space without Molecular Processing

Nagata, Yuji, Futamura, Akiko, Miyauchi, Keisuke, Takagi, Masamichi

γ-Hexachlorocyclohexane (γ-HCH) is one of several highly chlorinated insecticides that cause serious environmental problems. The cellular proteins of a γ-HCH-degrading bacterium, Sphingomonas...

Cloning and Sequencing of a Novel meta-Cleavage Dioxygenase Gene Whose Product Is Involved in Degradation of γ-Hexachlorocyclohexane in Sphingomonas paucimobilis

Miyauchi, Keisuke, Adachi, Yugo, Nagata, Yuji, Takagi, Masamichi

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide, as a sole source of carbon and energy. In a previous study, we...

Cloning and Sequencing of a 2,5-Dichlorohydroquinone Reductive Dehalogenase Gene Whose Product Is Involved in Degradation of γ-Hexachlorocyclohexane by Sphingomonas paucimobilis

Miyauchi, Keisuke, Suh, Seug-Kyo, Nagata, Yuji, Takagi, Masamichi

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide, as a sole carbon and energy source. In a previous study, we showed...

Isolation and Characterization of EPD1, an Essential Gene for Pseudohyphal Growth of a Dimorphic Yeast, Candida maltosa

Nakazawa, Takanobu, Horiuchi, Hiroyuki, Ohta, Akinori, Takagi, Masamichi

Additional copies of the centromeric DNA (CEN) region induce pseudohyphal growth in a dimorphic yeast, Candida maltosa (T. Nakazawa, T. Motoyama, H. Horiuchi, A. Ohta, and M. Takagi, J. Bacteriol....

Cloning and Characterization of linR, Involved in Regulation of the Downstream Pathway for γ-Hexachlorocyclohexane Degradation in Sphingomonas paucimobilis UT26

Miyauchi, Keisuke, Lee, Haeng-Seog, Fukuda, Masao, Takagi, Masamichi, Nagata, Yuji

In Sphingomonas paucimobilis UT26, LinD and LinE activities, which are responsible for the degradation of γ-hexachlorocyclohexane, are inducibly expressed in the presence of their substrates,...

Novel Approach to the Improvement of Biphenyl and Polychlorinated Biphenyl Degradation Activity: Promoter Implantation by Homologous Recombination

Ohtsubo, Yoshiyuki, Shimura, Minoru, Delawary, Mina, Kimbara, Kazuhide, Takagi, Masamichi, Kudo, Toshiaki, ...

To improve the capabilities of microorganisms relevant for biodegradation, we developed a new genetic approach and applied it to the bph operon (bphEGF[orf4]A1A2A3CD[orf1]A4R) of Pseudomonas sp....

A candidate prostate cancer susceptibility gene encodes tRNA 3′ processing endoribonuclease

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

tRNA 3′ processing endoribonuclease (3′ tRNase) is an enzyme responsible for the removal of a 3′ trailer from precursor tRNA (pre-tRNA). We purified ∼85 kDa 3′ tRNase from pig liver and...

A novel 4-base-recognizing RNA cutter that can remove the single 3′ terminal nucleotides from RNA molecules

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

Mammalian tRNase ZL shows versatility in substrate recognition. This enzyme can not only process pre-tRNAs by cleaving off their 3′ trailer sequences, but also recognize and cleave pre-tRNA-like...

The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

Transfer RNA (tRNA) 3′ processing endoribonuclease (tRNase Z) is an enzyme responsible for the removal of a 3′ trailer from pre-tRNA. There exists two types of tRNase Z: one is a short form...

Identification by Mn2+ rescue of two residues essential for the proton transfer of tRNase Z catalysis

Minagawa, Asako, Takaku, Hiroaki, Ishii, Ryohei, Takagi, Masamichi, Yokoyama, Shigeyuki, Nashimoto, Masayuki

Thermotoga maritima tRNase Z cleaves pre-tRNAs containing the 74CCA76 sequence precisely after the A76 residue to create the mature 3′ termini. Its crystal structure has revealed a four-layer...

Proliferation of Intrahyphal Hyphae Caused by Disruption of csmA, Which Encodes a Class V Chitin Synthase with a Myosin Motor-Like Domain in Aspergillus nidulans

Horiuchi, Hiroyuki, Fujiwara, Makoto, Yamashita, Shuichi, Ohta, Akinori, Takagi, Masamichi

We have found that the Aspergillus nidulans csmA gene encodes a novel protein which consists of an N-terminal myosin motor-like domain and a C-terminal chitin synthase domain (M. Fujiwara, H....

Two Different Types of Dehalogenases, LinA and LinB, Involved in γ-Hexachlorocyclohexane Degradation in Sphingomonas paucimobilis UT26 Are Localized in the Periplasmic Space without Molecular Processing

Nagata, Yuji, Futamura, Akiko, Miyauchi, Keisuke, Takagi, Masamichi

γ-Hexachlorocyclohexane (γ-HCH) is one of several highly chlorinated insecticides that cause serious environmental problems. The cellular proteins of a γ-HCH-degrading bacterium, Sphingomonas...

Cloning and Sequencing of a Novel meta-Cleavage Dioxygenase Gene Whose Product Is Involved in Degradation of γ-Hexachlorocyclohexane in Sphingomonas paucimobilis

Miyauchi, Keisuke, Adachi, Yugo, Nagata, Yuji, Takagi, Masamichi

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide, as a sole source of carbon and energy. In a previous study, we...

Cloning and Sequencing of a 2,5-Dichlorohydroquinone Reductive Dehalogenase Gene Whose Product Is Involved in Degradation of γ-Hexachlorocyclohexane by Sphingomonas paucimobilis

Miyauchi, Keisuke, Suh, Seug-Kyo, Nagata, Yuji, Takagi, Masamichi

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide, as a sole carbon and energy source. In a previous study, we showed...

Isolation and Characterization of EPD1, an Essential Gene for Pseudohyphal Growth of a Dimorphic Yeast, Candida maltosa

Nakazawa, Takanobu, Horiuchi, Hiroyuki, Ohta, Akinori, Takagi, Masamichi

Additional copies of the centromeric DNA (CEN) region induce pseudohyphal growth in a dimorphic yeast, Candida maltosa (T. Nakazawa, T. Motoyama, H. Horiuchi, A. Ohta, and M. Takagi, J. Bacteriol....

Cloning and Characterization of linR, Involved in Regulation of the Downstream Pathway for γ-Hexachlorocyclohexane Degradation in Sphingomonas paucimobilis UT26

Miyauchi, Keisuke, Lee, Haeng-Seog, Fukuda, Masao, Takagi, Masamichi, Nagata, Yuji

In Sphingomonas paucimobilis UT26, LinD and LinE activities, which are responsible for the degradation of γ-hexachlorocyclohexane, are inducibly expressed in the presence of their substrates,...

Novel Approach to the Improvement of Biphenyl and Polychlorinated Biphenyl Degradation Activity: Promoter Implantation by Homologous Recombination

Ohtsubo, Yoshiyuki, Shimura, Minoru, Delawary, Mina, Kimbara, Kazuhide, Takagi, Masamichi, Kudo, Toshiaki, ...

To improve the capabilities of microorganisms relevant for biodegradation, we developed a new genetic approach and applied it to the bph operon (bphEGF[orf4]A1A2A3CD[orf1]A4R) of Pseudomonas sp....

A candidate prostate cancer susceptibility gene encodes tRNA 3′ processing endoribonuclease

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

tRNA 3′ processing endoribonuclease (3′ tRNase) is an enzyme responsible for the removal of a 3′ trailer from precursor tRNA (pre-tRNA). We purified ∼85 kDa 3′ tRNase from pig liver and...

A novel 4-base-recognizing RNA cutter that can remove the single 3′ terminal nucleotides from RNA molecules

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

Mammalian tRNase ZL shows versatility in substrate recognition. This enzyme can not only process pre-tRNAs by cleaving off their 3′ trailer sequences, but also recognize and cleave pre-tRNA-like...

The N-terminal half-domain of the long form of tRNase Z is required for the RNase 65 activity

Takaku, Hiroaki, Minagawa, Asako, Takagi, Masamichi, Nashimoto, Masayuki

Transfer RNA (tRNA) 3′ processing endoribonuclease (tRNase Z) is an enzyme responsible for the removal of a 3′ trailer from pre-tRNA. There exists two types of tRNase Z: one is a short form...

Identification by Mn2+ rescue of two residues essential for the proton transfer of tRNase Z catalysis

Minagawa, Asako, Takaku, Hiroaki, Ishii, Ryohei, Takagi, Masamichi, Yokoyama, Shigeyuki, Nashimoto, Masayuki

Thermotoga maritima tRNase Z cleaves pre-tRNAs containing the 74CCA76 sequence precisely after the A76 residue to create the mature 3′ termini. Its crystal structure has revealed a four-layer...

The structure of the flexible arm of Thermotoga maritima tRNase Z differs from those of homologous enzymes

Ishii, Ryohei, Minagawa, Asako, Takaku, Hiroaki, Takagi, Masamichi, Nashimoto, Masayuki, Yokoyama, Shigeyuki

The crystal structure of T. maritima tRNase Z has been determined at 1.97 Å. The structure shows the flexible arm and the fully metal-binding active site.