Scientific Commons: Masaru Nasu

Effect of Helicobacter pylori infection on the N-methyl-N'-nitro-N-nitrosoguanidine-induced gastric carcinogenesis in Mongolian gerbils (1999)

Tokieda, Masashi, Honda, Shoji, Fujioka, Toshio, Nasu, Masaru

The effect of Helicobacter pylori infection on N-methyl-N′-nitro-N-nitrosoguanidine (MNNG)-induced gastric cancer was studied using a Mongolian gerbil model. Five-week-old male Mongolian gerbils...

PCR Detection of DNA Specific for Trichosporon Species in Serum of Patients with Disseminated Trichosporonosis

Nagai, Hiroyuki, Yamakami, Yuriko, Hashimoto, Atsuro, Tokimatsu, Issei, Nasu, Masaru

Deep-seated trichosporonosis is a lethal opportunistic infection that disseminates rapidly and widely in immunocompromised patients, and early diagnosis is crucial for the treatment of this...

Experimental Model of Progressive Disseminated Trichosporonosis in Mice with Latent Trichosporonemia

Yamagata, Eiji, Kamberi, Perparim, Yamakami, Yuriko, Hashimoto, Atsuro, Nasu, Masaru

Trichosporon asahii and Trichosporon mucoides are the most common strains of fungi that cause disseminated trichosporonosis, a severe opportunistic infection in immunocompromised hosts. We have...

Antimicrobial Susceptibilities of Helicobacter pylori Isolates under Microaerophilic Atmospheres Established by Two Different Methods

Kobayashi, Intetsu, Muraoka, Hiroe, Saika, Takeshi, Nishida, Minoru, Fujioka, Toshio, Nasu, Masaru

The MICs of clarithromycin, amoxicillin, and metronidazole for 150 Helicobacter pylori isolates were determined using the AnaeroPack system and were compared with those determined using a...

Clindamycin Suppresses Endotoxin Released by Ceftazidime-Treated Escherichia coli O55:B5 and Subsequent Production of Tumor Necrosis Factor Alpha and Interleukin-1β

Kishi, Kenji, Hirai, Kazuhiro, Hiramatsu, Kazufumi, Yamasaki, Tohru, Nasu, Masaru

Treatment of septicemia caused by Escherichia coli with ceftazidime (CAZ) may be associated with the development of septic shock due to the release of bacterial lipopolysaccharide. We examined the...

Pretreatment of Mice with Clindamycin Improves Survival of Endotoxic Shock by Modulating the Release of Inflammatory Cytokines

Hirata, Norio, Hiramatsu, Kazufumi, Kishi, Kenji, Yamasaki, Tohru, Ichimiya, Tomoku, Nasu, Masaru

Suppression of endotoxin release and subsequent production of inflammatory cytokines is crucial in the treatment of septic shock. We investigated the effect of clindamycin (CLI) on endotoxic shock...

Serological Assessment of the Early Response to Eradication Therapy Using an Immunodominant Outer Membrane Protein of Helicobacter pylori

Nishizono, Akira, Gotoh, Takayuki, Fujioka, Toshio, Murakami, Kazunari, Kubota, Toshihiro, Nasu, Masaru, ...

Eradication of Helicobacter pylori infection cures gastritis and prevents recurrence of peptic ulcers. Endoscopy is usually used to evaluate the effectiveness of eradication therapy. We designed a...

Comparison of Genomic Structures and Antigenic Reactivities of Orthologous 29-Kilodalton Outer Membrane Proteins of Helicobacter pylori

Sumie, Akihiro, Yamashiro, Tetsu, Nakashima, Kazutoshi, Nasu, Masaru, Watanabe, Makoto, Nishizono, Akira

We purified a 29-kDa Helicobacter pylori outer membrane protein (Omp29 protein) and cloned the gene encoding the protein from H. pylori strain ATCC 43504. The Omp29 gene corresponded to the reported...

Evaluation of PCR for Detection of DNA Specific for Aspergillus Species in Sera of Patients with Various Forms of Pulmonary Aspergillosis

Yamakami, Yuriko, Hashimoto, Atsuro, Yamagata, Eiji, Kamberi, Perparim, Karashima, Reiko, Nagai, Hiroyuki, ...

Pulmonary aspergillosis is classified into invasive, saprophytic, and allergic forms. In this study, we evaluated the usefulness of PCR for differentiating between different forms of aspergillosis or...

Diversity in Protein Synthesis and Viability of Helicobacter pylori Coccoid Forms in Response to Various Stimuli

Mizoguchi, Hiromoto, Fujioka, Toshio, Kishi, Kenji, Nishizono, Akira, Kodama, Reiji, Nasu, Masaru

The viability of the coccoid forms of Helicobacter pylori was evaluated by assessing protein synthesis. Metabolic labeling studies showed the synthesis of proteins and the specific protein profiles...

Local Secretory Immunoglobulin A and Postimmunization Gastritis Correlate with Protection against Helicobacter pylori Infection after Oral Vaccination of Mice

Goto, Takayuki, Nishizono, Akira, Fujioka, Toshio, Ikewaki, Junko, Mifune, Kumato, Nasu, Masaru

C57BL/6 mice were orally immunized with five weekly doses of 2 mg, 200 μg, or 2 μg of Helicobacter pylori (Sydney strain) whole-cell sonicate combined with cholera toxin. One week after the last...

Clindamycin Modulates Inflammatory-Cytokine Induction in Lipopolysaccharide-Stimulated Mouse Peritoneal Macrophages

Nakano, Tetsuji, Hiramatsu, Kazufumi, Kishi, Kenji, Hirata, Norio, Kadota, Jun-ichi, Nasu, Masaru

We investigated the mechanism by which clindamycin (CLI) modulates cytokine induction after lipopolysaccharide (LPS) stimulation. Although CLI decreased the intracellular expression levels of tumor...

Real-Time PCR Assay Compared to Nested PCR and Antigenemia Assays for Detecting Cytomegalovirus Reactivation in Adult T-Cell Leukemia-Lymphoma Patients

Ikewaki, Junji, Ohtsuka, Eiichi, Kawano, Rie, Ogata, Masao, Kikuchi, Hiroshi, Nasu, Masaru

We analyzed the efficiency of the quantitative real-time PCR assay for cytomegalovirus (CMV) reactivation in adult T-cell leukemia-lymphoma (ATL) patients and compared the results with those obtained...

Effect of Sucralfate on Antibiotic Therapy for Helicobacter pylori Infection in Mice

Watanabe, Koichiro, Murakami, Kazunari, Sato, Ryugo, Kashimura, Koji, Miura, Masahiro, Ootsu, Satoshi, ...

It has been documented that sucralfate, a basic aluminum salt, enhances the efficacies of antibiotics against Helicobacter pylori, resulting in eradication rates comparable to those associated with...