Masaru Tomita

A new multicompartmental reaction-diffusion modeling method links transient membrane attachment of E. coli MinE to E-ring formation (2009)

Masaru Tomita

Many important cellular processes are regulated by reaction-diffusion (RD) of molecules that takes place both in the cytoplasm and on the membrane. To model and analyze such multicompartmental...

A web server for interactive and zoomable Chaos Game Representation images (2009)

Arakawa, Kazuharu, Oshita, Kazuki, Tomita, Masaru

Abstract Chaos Game Representation (CGR) is a generalized scale-independent Markov transition table, which is useful for the visualization and comparative study of genomic signature, or for the study...

Modeling reaction-diffusion of molecules on surface and in volume spaces with the E-Cell System (2009)

Masaru Tomita

The-Cell System is an advanced open-source simulation platform to model and analyze biochemical reaction networks. The present algorithm modules of the system assume that the reacting molecules are...

Measure of synonymous codon usage diversity among genes in bacteria (2009)

Suzuki, Haruo, Saito, Rintaro, Tomita, Masaru

Abstract Background In many bacteria, intragenomic diversity in synonymous codon usage among genes has been reported. However, no quantitative attempt has been made to compare the diversity levels...

A Software Engineering Approach (2009)

Yohei Yamada, Masaru Tomita

cellular processes involves diverse components and complex interactions. To successfully model such processes, simulation systems must meet a number of computational requirements, including an...

Genome Projector: zoomable genome map with multiple views (2009)

Arakawa, Kazuharu, Tamaki, Satoshi, Kono, Nobuaki, Kido, Nobuhiro, Ikegami, Keita, Ogawa, Ryu, ...

Abstract Background Molecular biology data exist on diverse scales, from the level of molecules to -omics. At the same time, the data at each scale can be categorised into multiple layers, such as...

Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus (2009)

Kanai, Akio, Sato, Asako, Fukuda, Yoko, Okada, Kiyoshi, Matsuda, Takashi, Sakamoto, Taiichi, ...

Using an expression protein library of a hyperthermophilic archaeon, Pyrococcus furiosus, we identified a gene (PF0027) that encodes a protein with heat-stable cyclic nucleotide phosphodiesterase...

GeNESiS: gene network evolution simulation software (2008)

Kratz, Anton, Tomita, Masaru, Krishnan, Arun

Abstract Background There has been a lot of interest in recent years focusing on the modeling and simulation of Gene Regulatory Networks (GRNs). However, the evolutionary mechanisms that give rise to...

Time-resolved metabolomics reveals metabolic modulation in rice foliage (2008)

Sato, Shigeru, Arita, Masanori, Soga, Tomoyoshi, Nishioka, Takaaki, Tomita, Masaru

Abstract Background To elucidate the interaction of dynamics among modules that constitute biological systems, comprehensive datasets obtained from "omics" technologies have been used. In recent...

Prediction of Protein Subcellular Localization Using an Artificial Neural Network (2008)

Anton Kratz, Rintaro Saito, Arun Krishnan, Masaru Tomita

Experimental methods for determining protein localization can not keep up with the speed at which genomic sequences are being generated. There has been extensive research in computational methods for...

Table of Contents (2008)

Masaru Tomita, Marion Kee, Hiroaki Saito, Teruko Mitamura, Hideto Tomabechi

We describe our Universal Parser Architecture and its use in a speech translation system, based on the Machine Translation system under development at the Center for Machine Translation at Carnegie...

312 Genome Informatics 12: 312–313 (2001) Towards Simulation of Whole Metabolic Pathways in (2008)

Human Erythrocyte, Ayako Kinoshita, Yoichi Nakayama, Masaru Tomita

We have previously reported the simulation of the glucose-6-phosphate dehydrogenase (G6PD) deficiency in human erythrocyte using E-CELL system. In this previous work, we showed that the glutathione...

Development of E-Cell kinetic model interoperability environment (2008)

Tatsuya Ishida, Takeshi Sakurada, Tomoya Kitayama, Masaru Tomita

Keywords: systems biology, model repository, cell simulation, the E-Cell system

Distributed Cell Biology Simulations with E-Cell System (2008)

Masahiro Sugimoto, Kouichi Takahashi, Tomoya Kitayama, Daiki Ito, Masaru Tomita

Abstract. Many useful applications of simulation in computational cell biology, e.g. kinetic parameter estimation, Metabolic Control Analysis (MCA), and bifurcation analysis, require a large number...

BIOINFORMATICS ORIGINAL PAPER (2008)

Genome Analysis, Noriyuki Kitagawa, Takanori Washio, Shunichi Kosugi, Tomoya Yamashita, Kenji Higashi, ...

Vol. 21 no. 9 2005, pages 1758–1763 doi:10.1093/bioinformatics/bti253 Computational analysis suggests that alternative first exons are involved in tissue-specific transcription in rice (Oryza...

RNAs in Drosophila Identification and expression analysis of putative mRNA-like non-coding RNA in Drosophila (2008)

Sachi Inagaki, Koji Numata, Takefumi Kondo, Masaru Tomita, Kunio Yasuda, Akio Kanai, ...

One of the most surprising results to emerge from mammalian cDNA sequencing projects is that thousands of mRNA-like non-coding RNAs (ncRNAs) are expressed and constitute at least 10% of poly(A) +...

Statistical Characterization of Transcription Start Sites (2008)

In Plant Genomes, Shigeo Fujimori, Takanori Washio, Masaru Tomita

Although large amounts of genomic and full-length cDNA sequence data from plants are now publicly available, knowledge of the promoters and transcription start sites (TSSs) in plants is still limited...

GANDivAWeb: A web server for detecting early folding units ("foldons") from protein 3D structures (2008)

Laborde, Thomas, Tomita, Masaru, Krishnan, Arun

Abstract Background It has long been known that small regions of proteins tend to fold independently and are then stabilized by interactions between these distinct subunits or modules. Such units,...

Computational Analysis of the Strand Bias of the Chi Sequences and Other Oligonucleotides in Bacterial (2008)

Kazuharu Arakawa, Reina Uno, Yoichi Nakayama, Masaru Tomita

A Chi sequence (5’-GCTGGTGG-3’) in Escherichia coli is an 8bp sequence element that promotes recombination by attenuating the activity of RecBCD enzyme. Upon recognition of the Chi sequence, the...

Appficafio ~ of the Direct Memory Access paradigm to (2008)

I~deto Comabechi, Masaru Tomita

~,ot ~ °~a ~ ~a~gl~age interfaces to knowledge-based systems

Genome Informatics 14: 394--395 (2003) Large Scale Analysis of Transcriptional and Translational Control Mechanism in Human Ribosomal Proteins (2008)

Kyota Ishii Tamayo, Tamayo Uechi, Takanori Washio, Maki Yoshihama, Naoya Kenmochi, Masaru Tomita

Introduction The human 80S ribosome was composed with 79 ribosomal proteins (RPs) and 4 rRNAs. Although all of RP genes have been thought to have an identical transcriptional and translational...

Genome Informatics 14: 294--295 (2003) E - Cell System Version 3: A Software Platform for (2008)

Integrative Computational Biology, Kouichi Takahashi, Takeshi Sakurada, Kazunari Kaizu, Tomoya Kitayama, Satya Arjunan, ...

Introduction E - Cell System is an OpenSource software environment for modeling, simulation and mathematical analysis of the cell (http://www.e-cell.org/software). Unlike its preceding versions, E -...

BMC Structural Biology (2008)

Thomas Laborde, Masaru Tomita, Arun Krishnan

Software GANDivAWeb: A web server for detecting early folding units ("foldons") from protein 3D structures

Aligning LC peaks by converting gradient retention times to retention index of peptides in proteomic experiments (2008)

Shinoda, Kosaku, Tomita, Masaru, Ishihama, Yasushi

Motivation: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful tool in proteomics studies, but when peptide retention information is used for identification purposes, it remains...

Reshuffling of the Bacillus subtilis 168 Genome by Multifold Inversion (2008)

Kuroki, Azusa, Toda, Tsutomu, Matsui, Kuniko, Uotsu-Tomita, Rie, Tomita, Masaru, Itaya, Mitsuhiro

The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires...

Comparative expression analysis uncovers novel features of endogenous antisense transcription (2008)

Okada, Yuki, Tashiro, Chiaki, Numata, Koji, Watanabe, Kazufumi, Nakaoka, Hajime, Yamamoto, Naoyuki, ...

Increasing numbers of sense–antisense transcripts (SATs), which are transcribed from the same chromosomal location but in opposite directions, have been identified in various eukaryotic species,...

Comprehensive Analysis of Archaeal tRNA Genes Reveals Rapid Increase of tRNA Introns in the Order Thermoproteales (2008)

Sugahara, Junichi, Kikuta, Kaoru, Fujishima, Kosuke, Yachie, Nozomu, Tomita, Masaru, Kanai, Akio

The analysis of archaeal tRNA genes is becoming more important to evaluate the origin and evolution of tRNA molecule. Even with the recent accumulation of complete genomes of numerous archaeal...

CMU MT ToolKit version 8-5 (2007)

Masaru Tomita

This paper describes a software package that consists of the following three major components:

Reviewed by (2007)

Sergei Nirenburg, Jaime Carbonell, Masaru Tomita, Kenneth Goodman, Kenneth Goodman, Sergei Nirenburg (editors, ...

Machine translation (MT) research in the United States has enjoyed a bit of a revival in the last several years. Building on research in semantic analysis from both natural language processing and...

460 Genome Informatics 11: 460–461 (2000) Computer Simulation of Enzyme Deficiency in Human Erythrocyte Using the E-Cell System (2007)

Yoichi Nakayama, Ayako Kinoshita, Masaru Tomita

We previously reported a computer model of the human erythrocyte using E-CELL simulation system [1, 2]. The model has three major metabolic pathways, including glycolysis, the pentose phosphate...

Computer Simulation of Glycolysis in Pancreatic β-Cells Using the E-CELL System (2007)

Masaru Tomita

All vertebrates use insulin-producing pancreatic β-cells to keep track of the levels of the predominant small nutrient molecules in blood by accurately measuring the concentrations of glucose, amino...

456 Genome Informatics 11: 456–457 (2000) Quantitative Modeling of Mitochondrial Energy Metabolism Using E-Cell Simulation Environment (2007)

Katsuyuki Yugi, Masaru Tomita

We have been constructing a kinetic model of mitochondrial energy metabolism using the E-Cell system [2, 3], a generic simulation environment we have developed for whole cell simulation. The model...

458 Genome Informatics 11: 458–459 (2000) Computer Modeling of Photosynthesis Using the E-CELL System (2007)

Emily Wang, Yoichi Nakayama, Masaru Tomita

The E-CELL system is a generic software package for whole cell modeling and simulation developed by the Laboratory for Bioinformatics at Keio University [1]. Photosynthesis is generally characterized...

Comprehensive Sequence Analyses of 5 ' Flanking Regions of Primate Alu Elements (2007)

Yoshimi Toda, Rintaro Saito, Masaru Tomita

Retrotransposons have been generally known to integrate randomly into host genomes. Jurka (1997) [3], however, showed consensus sequence patterns at integration sites of certain mammalian...

Analysis of Translation Initiation Sites in the Complete Genome of Mycoplasma genitalium Suggests That This Bacterium Uses Alternative Signal for Translation Initiation (2007)

Rintaro Saito, Masaru Tomita

Although the Shine-Dalgarno (SD) sequence [5] is widely accepted as the signal sequence for ribosomemRNA binding in procaryotic translation initiation, this sequence is not well conserved among genes...

Elements (2007)

Yoshimi Toda, Rintaro Saito, Masaru Tomita

Retrotransposons have been generally known to integrate randomly into host genomes. Jurka (1997) [3], however, showed consensus sequence patterns at integration sites of certain mammalian...

Development of Bacteria Analysis System v.2 on the G-Language Genome Analysis Environment (2007)

Daisuke Kyuma, Kazuharu Arakawa, Koya Mori, Masaru Tomita

Keywords: G-language Genome Analysis Environment, analysis software, computational analysis

Automatic Generation of Cell-Wide Pathway Model from Complete Genome (2007)

Kazuharu Arakawa, Yohei Yamada, Hiromi Komai, Kosaku Shinoda, Yoichi Nakayama, Masaru Tomita

Knowledge in molecular biology is rapidly accumulating in the fields of genome, transcriptome, proteome, and metabolome, demanding for a systems biology approach in order to view the dynamic behavior...

Model-based Definition of Population Heterogeneity and Its Effects on Metabolism in Sporulating Bacillus subtilis (2007)

Morohashi, Mineo, Ohashi, Yoshiaki, Tani, Saeka, Ishii, Kotaro, Itaya, Mitsuhiro, Nanamiya, Hideaki, ...

The soil bacterium Bacillus subtilis forms dormant, robust spores as a tactic to ensure survival under conditions of starvation. However, the sporulating culture includes sporulating and...

Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genes (2007)

Kochiwa, Hiromi, Tomita, Masaru, Kanai, Akio

Abstract Background A theoretical model of genetic redundancy has proposed that the fates of redundant genes depend on the degree of functional redundancy, and that functionally redundant genes will...

Proteome-Wide Prediction of Novel DNA/RNA-Binding Proteins Using Amino Acid Composition and Periodicity in the Hyperthermophilic Archaeon Pyrococcus furiosus (2007)

Fujishima, Kosuke, Komasa, Mizuki, Kitamura, Sayaka, Suzuki, Haruo, Tomita, Masaru, Kanai, Akio

Proteins play a critical role in complex biological systems, yet about half of the proteins in publicly available databases are annotated as functionally unknown. Proteome-wide functional...

Consideration on Contact Mechanism of YBaCuO Bulk Superconductor with Deposited Metal Layer (2007)

FUJITA, Hiroyuki, FUKUDA, Katsuya, SAWA, Koichiro, TOMITA, Masaru, MURAKAMI, Masato, SAKAI, Naomichi, ...

A persistent current switch (PCS) is used for superconducting applications, such as superconducting magnetic energy storage (SMES) system. The authors have proposed a mechanical switch of Y-Ba-Cu-O...

Distinguishing enzymes using metabolome data for the hybrid dynamic/static method (2007)

Ishii, Nobuyoshi, Nakayama, Yoichi, Tomita, Masaru

Abstract Background In the process of constructing a dynamic model of a metabolic pathway, a large number of parameters such as kinetic constants and initial metabolite concentrations are required....

In silico screening of archaeal tRNA-encoding genes having multiple introns with bulge-helix-bulge splicing motifs (2007)

Sugahara, Junichi, Yachie, Nozomu, Arakawa, Kazuharu, Tomita, Masaru

In archaeal species, several transfer RNA genes have been reported to contain endogenous introns. Although most of the introns are located at anticodon loop regions between nucleotide positions 37...

Visualization of three-way comparisons of omics data (2007)

Baran, Richard, Robert, Martin, Suematsu, Makoto, Soga, Tomoyoshi, Tomita, Masaru

Abstract Background Density plot visualizations (also referred to as heat maps or color maps) are widely used in different fields including large-scale omics studies in biological sciences. However,...

Variation in the Correlation of G + C Composition with Synonymous Codon Usage Bias among Bacteria (2007)

Haruo Suzuki, Rintaro Saito, Masaru Tomita

G + C composition at the third codon position (GC3) is widely reported to be correlated with synonymous codon usage bias. However, no quantitative attempt has been made to compare the extent of this...

Selection Effects on the Positioning of Genes and Gene Structures from the Interplay of Replication and Transcription in Bacterial Genomes (2007)

Kazuharu Arakawa, Masaru Tomita

Bacterial chromosomes are partly shaped by the functional requirements for efficient replication, which lead to strand bias as commonly characterized by the excess of guanines over cytosines in the...

The GC Skew Index: A Measure of Genomic Compositional Asymmetry and the Degree of Replicational Selection (2007)

Kazuharu Arakawa, Masaru Tomita

Circular bacterial chromosomes have highly polarized nucleotide composition in the two replichores, and this genomic strand asymmetry can be visualized using GC skew graphs. Here we propose and...

The GC Skew Index: A Measure of Genomic Compositional Asymmetry and the Degree of Replicational Selection (2007)

Kazuharu Arakawa, Masaru Tomita

Circular bacterial chromosomes have highly polarized nucleotide composition in the two replichores, and this genomic strand asymmetry can be visualized using GC skew graphs. Here we propose and...

Variation in the Correlation of G + C Composition with Synonymous Codon Usage Bias among Bacteria (2007)

Haruo Suzuki, Rintaro Saito, Masaru Tomita

G + C composition at the third codon position (GC3) is widely reported to be correlated with synonymous codon usage bias. However, no quantitative attempt has been made to compare the extent of this...

In silico screening of archaeal tRNA-encoding genes having multiple introns with bulge-helix-bulge splicing motifs (2007)

Sugahara, Junichi, Yachie, Nozomu, Arakawa, Kazuharu, Tomita, Masaru

In archaeal species, several transfer RNA genes have been reported to contain endogenous introns. Although most of the introns are located at anticodon loop regions between nucleotide positions 37...

MathDAMP: a package for differential analysis of metabolite profiles (2006)

Baran, Richard, Kochi, Hayataro, Saito, Natsumi, Suematsu, Makoto, Soga, Tomoyoshi, Nishioka, Takaaki, ...

Abstract Background With the advent of metabolomics as a powerful tool for both functional and biomarker discovery, the identification of specific differences between complex metabolite profiles is...

HybGFS: a hybrid method for genome-fingerprint scanning (2006)

Shinoda, Kosaku, Yachie, Nozomu, Masuda, Takeshi, Sugiyama, Naoyuki, Sugimoto, Masahiro, Soga, Tomoyoshi, ...

Abstract Background Protein identification based on mass spectrometry (MS) has previously been performed using peptide mass fingerprinting (PMF) or tandem MS (MS/MS) database searching. However,...

A simplified method for power-law modelling of metabolic pathways from time-course data and steady-state flux profiles (2006)

Kitayama, Tomoya, Kinoshita, Ayako, Sugimoto, Masahiro, Nakayama, Yoichi, Tomita, Masaru

Abstract Background In order to improve understanding of metabolic systems there have been attempts to construct S-system models from time courses. Conventionally, non-linear curve-fitting algorithms...

Parameter estimation for stiff equations of biosystems using radial basis function networks (2006)

Matsubara, Yoshiya, Kikuchi, Shinichi, Sugimoto, Masahiro, Tomita, Masaru

Abstract Background The modeling of dynamic systems requires estimating kinetic parameters from experimentally measured time-courses. Conventional global optimization methods used for parameter...

GEM System: automatic prototyping of cell-wide metabolic pathway models from genomes (2006)

Arakawa, Kazuharu, Yamada, Yohei, Shinoda, Kosaku, Nakayama, Yoichi, Tomita, Masaru

Abstract Background Successful realization of a "systems biology" approach to analyzing cells is a grand challenge for our understanding of life. However, current modeling approaches to cell...

Characteristics and clustering of human ribosomal protein genes (2006)

Ishii, Kyota, Washio, Takanori, Uechi, Tamayo, Yoshihama, Maki, Kenmochi, Naoya, Tomita, Masaru

Abstract Background The ribosome is a central player in the translation system, which in mammals consists of four RNA species and 79 ribosomal proteins (RPs). The control mechanisms of gene...

Evaluation of Metabolic Alteration in Transgenic Rice Overexpressing Dihydroflavonol-4-reductase (2006)

TAKAHASHI, HIDEYUKI, HAYASHI, MITSUNORI, GOTO, FUMIYUKI, SATO, SHIGERU, SOGA, TOMOYOSHI, NISHIOKA, TAKAAKI, ...

• Background and Aims Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress...

Large-scale identification of protein-protein interaction of Escherichia coli K-12 (2006)

Arifuzzaman, Mohammad, Maeda, Maki, Itoh, Aya, Nishikata, Kensaku, Takita, Chiharu, Saito, Rintaro, ...

Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular...

Evaluation of Metabolic Alteration in Transgenic Rice Overexpressing Dihydroflavonol-4-reductase (2006)

TAKAHASHI, HIDEYUKI, HAYASHI, MITSUNORI, GOTO, FUMIYUKI, SATO, SHIGERU, SOGA, TOMOYOSHI, NISHIOKA, TAKAAKI, ...

• Background and Aims Previous studies have shown that transgenic rice plants overexpressing YK1, which possesses dihydroflavonol-4-reductase (DFR) activity, showed biotic and abiotic stress...

Large-scale identification of protein-protein interaction of Escherichia coli K-12 (2006)

Arifuzzaman, Mohammad, Maeda, Maki, Itoh, Aya, Nishikata, Kensaku, Takita, Chiharu, Saito, Rintaro, ...

Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular...

A microarray data-based semi-kinetic method for predicting quantitative dynamics of genetic networks (2005)

Yugi, Katsuyuki, Nakayama, Yoichi, Kojima, Shigen, Kitayama, Tomoya, Tomita, Masaru

Abstract Background Elucidating the dynamic behaviour of genetic regulatory networks is one of the most significant challenges in systems biology. However, conventional quantitative predictions have...

Hybrid dynamic/static method for large-scale simulation of metabolism (2005)

Yugi, Katsuyuki, Nakayama, Yoichi, Kinoshita, Ayako, Tomita, Masaru

Abstract Background Many computer studies have employed either dynamic simulation or metabolic flux analysis (MFA) to predict the behaviour of biochemical pathways. Dynamic simulation determines the...

Dynamic simulation of red blood cell metabolism and its application to the analysis of a pathological condition (2005)

Nakayama, Yoichi, Kinoshita, Ayako, Tomita, Masaru

Abstract Background Cell simulation, which aims to predict the complex and dynamic behavior of living cells, is becoming a valuable tool. In silico models of human red blood cell (RBC) metabolism...

GC-compositional strand bias around transcription start sites in plants and fungi (2005)

Fujimori, Shigeo, Washio, Takanori, Tomita, Masaru

Abstract Background A GC-compositional strand bias or GC-skew (=(C-G)/(C+G)), where C and G denote the numbers of cytosine and guanine residues, was recently reported near the transcription start...

BMC Bioinformatics BioMed Central (2005)

Katsuyuki Yugi, Yoichi Nakayama, Shigen Kojima, Tomoya Kitayama, Masaru Tomita

Research article A microarray data-based semi-kinetic method for predicting quantitative dynamics of genetic networks

Space in systems biology of signaling pathways—towards intracellular molecular crowding in silico (2005)

Kouichi Takahashi, Satya N, A Vel Arjunan, Masaru Tomita

Abstract How cells utilize intracellular spatial features to optimize their signaling characteristics is still not clearly understood. The physical distance between the cell-surface receptor and the...

BMC Bioinformatics BioMed Central (2005)

Masaru Tomita

Research article Parameter estimation for stiff equations of biosystems using radial basis function networks

Computational analysis suggests that alternative first exons are involved in tissue-specific transcription in rice (Oryza sativa) (2005)

Kitagawa, Noriyuki, Washio, Takanori, Kosugi, Shunichi, Yamashita, Tomoya, Higashi, Kenji, Yanagawa, Hiroshi, ...

Motivation: Transcription start site selection and alternative splicing greatly contribute to diversifying gene expression. Recent studies have revealed the existence of alternative first exons, but...

Computational analysis suggests that alternative first exons are involved in tissue-specific transcription in rice (Oryza sativa) (2005)

Kitagawa, Noriyuki, Washio, Takanori, Kosugi, Shunichi, Yamashita, Tomoya, Higashi, Kenji, Yanagawa, Hiroshi, ...

Motivation: Transcription start site selection and alternative splicing greatly contribute to diversifying gene expression. Recent studies have revealed the existence of alternative first exons, but...

A Multi-Algorithm, Multi-Timescale Method for Cell Simulation (2004)

Kouichi Takahashi, Kazunari Kaizu, Bin Hu, Masaru Tomita

Motivation Many important problems in cell biology require the dense nonlinear interactions between functional modules to be considered. The importance of computer simulation in understanding...

A multi-algorithm, multi-timescale method for cell simulation (2004)

Takahashi, Kouichi, Kaizu, Kazunari, Hu, Bin, Tomita, Masaru

Motivation Many important problems in cell biology require the dense nonlinear interactions between functional modules to be considered. The importance of computer simulation in understanding...

A general computational model of mitochondrial metabolism in a whole organelle scale (2004)

Yugi, Katsuyuki, Tomita, Masaru

Summary: A computational tool for mitochondrial systems biology has been developed as a simulation model of ECell2, a publicly available simulation system. The general model consists of 58 enzymatic...

A multi-algorithm, multi-timescale method for cell simulation (2004)

Takahashi, Kouichi, Kaizu, Kazunari, Hu, Bin, Tomita, Masaru

Motivation: Many important problems in cell biology require the dense nonlinear interactions between functional modules to be considered. The importance of computer simulation in understanding...

Computational comparative analyses of alternative splicing regulation using full-length cDNA of various eukaryotes (2004)

ITOH, HITOMI, WASHIO, TAKANORI, TOMITA, MASARU

We previously reported a computational approach to infer alternative splicing patterns from Mus musculus full-length cDNA clones and microarray data. Although we predicted a large number of...

A general computational model of mitochondrial metabolism in a whole organelle scale (2004)

Yugi, Katsuyuki, Tomita, Masaru

Summary: A computational tool for mitochondrial systems biology has been developed as a simulation model of E-Cell2, a publicly available simulation system. The general model consists of 58 enzymatic...

Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7 (2004)

Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro

ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and...

A multi-algorithm, multi-timescale method for cell simulation (2004)

Takahashi, Kouichi, Kaizu, Kazunari, Hu, Bin, Tomita, Masaru

Motivation Many important problems in cell biology require the dense nonlinear interactions between functional modules to be considered. The importance of computer simulation in understanding...

A general computational model of mitochondrial metabolism in a whole organelle scale (2004)

Yugi, Katsuyuki, Tomita, Masaru

Summary: A computational tool for mitochondrial systems biology has been developed as a simulation model of ECell2, a publicly available simulation system. The general model consists of 58 enzymatic...

Translational Efficiency Prediction Using Structural Learning Method (2003)

Koya Mori, Rintaro Saito, Shinichi Kikuchi, Masaru Tomita

It has been shown that sequence patterns including Shine-Dalgarno sequences, start codons and those in between have effect on translational efficiencies. However relationships between these patterns...

Model Construction of the Energy Metabolism Pathway in Myocardial Cell (2003)

In Myocardial Cell, Motohiro Yoneda, Shoko Miyamoto, Yasuhiro Naito, Sayaka Ishinabe, Satoshi Matsuoka, ...

Introduction Myocardial cell has simple outputs, such as membrane potential and muscle contraction, which integrate many elementary processes, and a vast quantity of data is available from the patch...

Integrated Distributed Computing Environment on the G-Language GAE v.2 (2003)

Ryo Hattori, Kazuharu Arakawa, Hayataro Kouchi, Masaru Tomita

this paper, we report the development and implementation of the distributed computing environment utilizing database management system and client-server architecture.In order to analyze the huge...

Identifying Potential Regulatory Sequences of Alternative Splicing (2003)

Hitomi Itoh, Takanori Washio, Masaru Tomita

Introduction Alternative splicing is an important mechanism that contributes to expanding protein diversity by generating multiple protein isoforms from a single gene. We have previously reported...

Microarray Visualization System on the G-Language GAE (2003)

Yohei Yamada, Kazuharu Arakawa, Hirotada Mori, Masaru Tomita

Introduction In this work, we have developed the Microarray Visualization System over the generic bioinformatics workbench G-language Genome Analysis Environment (G-language GAE) [5] which...

G-Language Genome Analysis Environment Version 2 (2003)

Hayataro Kouchi, Kazuharu Arakawa, Ryo Hattori, Yohei Yamada, Yusuke Kobayashi, Atsuko Kishi, ...

Introduction G-language Project of Institute for Advanced Biosciences aims to introduce higher e#ciency in genome analysis by: 1. Constructing an integrated environment for the development of...

Identification of Non-Coding RNAs in the Escherichia coli Genome Using Sequence Specificity Index (SSI) (2003)

Nozomu Yachie, Koji Numata, Rintaro Saito, Akio Kanai, Masaru Tomita

Introduction Non-coding RNAs (ncRNAs) are thought to act as structural, regulatory or catalytic molecules, while messenger RNAs (mRNAs) are intermediated RNA molecules, which are translated to...

Identification of Putative Noncoding RNAs Among the RIKEN Mouse Full-Length cDNA Collection (2003)

Numata, Koji, Kanai, Akio, Saito, Rintaro, Kondo, Shinji, Adachi, Jun, Wilming, Laurens G., ...

With the sequencing and annotation of genomes and transcriptomes of several eukaryotes, the importance of noncoding RNA (ncRNA)—RNA molecules that are not translated to protein products—has...

Dynamic modeling of genetic networks using genetic algorithm and S-system (2003)

Kikuchi, Shinichi, Tominaga, Daisuke, Arita, Masanori, Takahashi, Katsutoshi, Tomita, Masaru

Motivation: The modeling of system dynamics of genetic networks, metabolic networks or signal transduction cascades from time-course data is formulated as a reverse-problem. Previous studies focused...

Computational analysis of stop codon readthrough in D.melanogaster (2003)

Sato, Misaki, Umeki, Hitomi, Saito, Rintaro, Kanai, Akio, Tomita, Masaru

Motivation: Readthrough is an unusual process in which a stop codon is misread or skipped. Recently it has been shown that some translation is regulated by the readthrough reactions although the...

Multivariate time series analysis of metabolic network using the E-CELL simulation system, Genome Informatics 1998, Universal Academy (1998)

Yusuke Saito, Kouichi Takahashi, Masaru Tomita

We applied multivariate time series analysis to metabolic models constructed using the E-CELL system. The E-CELL system [1] is a generic simulation environment for modeling cellular processes at...

Efficient Generalized LR Parsing of Word Lattices (1993)

Alon Lavie, Masaru Tomita

A word lattice is an efficient representation of a large set of possible sentence candidates, of which only a few are grammaticaland thus parsable. Word lattices are a common output of some speech...

GLR* -- An Efficient Noise-skipping Parsing Algorithm For Context Free Grammars (1993)

Alon Lavie And, Alon Lavie, Masaru Tomita

This chapter describes GLR*, a parser that can parse any input sentence by ignoring unrecognizable parts of the sentence. Using an efficient algorithm, the parser is capable of finding and parsing a...

GLR* - An Efficient Noise-skipping Parsing Algorithm For Context Free Grammars (1993)

Alon Lavie, Masaru Tomita

This paper describes GLR*, a parser that can parse any input sentence by ignoring unrecognizable parts of the sentence. In case the standard parsing procedure fails to parse an input sentence, the...

GLR* - An Efficient Noise-skipping Parsing Algorithm For Context Free Grammars (1993)

Alon Lavie, Masaru Tomita

This chapter describes GLR*, a parser that can parse any input sentence by ignoring unrecognizable parts of the sentence. Using an efficient algorithm, the parser is capable of finding and parsing a...

The Generalized LR Parser/Compiler V8-4: A Software Package for Practical NL Projects (1990)

Masaru Tomita

This paperldescribes a software package designed for practical projects which involve natural language parsing.]he Generalized LR Parser/Compiler V8-4 is based on Tomita's Generalized LR Parsing...

Natural-language understanding (1987)

Philip J. Hayes, Er G. Hauptmann, Jaime G. Carbonell, Masaru Tomita

Parsing spoken input introduces serious problems not present in parsing typed natural language. In particular, indeterminacies and inaccuracies of acoustic recognition must be handled in an integral...

An efficient augmented-context-free parsing algorithm (1987)

Masaru Tomita

An efficient parsing algorithm for augmented context-free grammars is introduced, and its application to on-line natural language interfaces discussed. The algorithm is a generalized LR parsing...

Inferring Alternative Splicing Patterns in Mouse from a Full-Length cDNA Library and Microarray Data

Kochiwa, Hiromi, Suzuki, Ryosuke, Washio, Takanori, Saito, Rintaro, Bono, Hidemasa, ...

Although many studies on alternative splicing of specific genes have been reported in the literature, the general mechanism that regulates alternative splicing has not been clearly understood. In...

Identification of Putative Noncoding RNAs Among the RIKEN Mouse Full-Length cDNA Collection

Numata, Koji, Kanai, Akio, Saito, Rintaro, Kondo, Shinji, Adachi, Jun, Wilming, Laurens G., ...

With the sequencing and annotation of genomes and transcriptomes of several eukaryotes, the importance of noncoding RNA (ncRNA)—RNA molecules that are not translated to protein products—has...

Comparative Analysis of Physical Maps of Four Bacillus subtilis (natto) Genomes

Qiu, Dongru, Fujita, Kyoko, Sakuma, Yuko, Tanaka, Teruo, Ohashi, Yoshiaki, Ohshima, Hideyuki, ...

The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome...

Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7

Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro

ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and...

Identification of the first archaeal Type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI can cleave an RNA–DNA junction

Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro

All the archaeal genomes sequenced to date contain a single Type 2 RNase H gene. We found that the genome of a halophilic archaeon, Halobacterium sp. NRC-1, contains an open reading frame with...

Computational comparative analyses of alternative splicing regulation using full-length cDNA of various eukaryotes

ITOH, HITOMI, WASHIO, TAKANORI, TOMITA, MASARU

We previously reported a computational approach to infer alternative splicing patterns from Mus musculus full-length cDNA clones and microarray data. Although we predicted a large number of...

Archaeal Pyrococcus furiosus thymidylate synthase 1 is an RNA-binding protein

Kanai, Akio, Sato, Asako, Imoto, Jun, Tomita, Masaru

Using a stem–loop RNA oligonucleotide (19-mer) containing an AUG sequence in the loop region as a probe, we screened the protein library from a hyperthermophilic archaeon, Pyrococcus furiosus, and...

Large-scale identification of protein–protein interaction of Escherichia coli K-12

Arifuzzaman, Mohammad, Maeda, Maki, Itoh, Aya, Nishikata, Kensaku, Takita, Chiharu, Saito, Rintaro, ...

Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular...

Inferring Alternative Splicing Patterns in Mouse from a Full-Length cDNA Library and Microarray Data

Kochiwa, Hiromi, Suzuki, Ryosuke, Washio, Takanori, Saito, Rintaro, Bono, Hidemasa, ...

Although many studies on alternative splicing of specific genes have been reported in the literature, the general mechanism that regulates alternative splicing has not been clearly understood. In...

Identification of Putative Noncoding RNAs Among the RIKEN Mouse Full-Length cDNA Collection

Numata, Koji, Kanai, Akio, Saito, Rintaro, Kondo, Shinji, Adachi, Jun, Wilming, Laurens G., ...

With the sequencing and annotation of genomes and transcriptomes of several eukaryotes, the importance of noncoding RNA (ncRNA)—RNA molecules that are not translated to protein products—has...

Comparative Analysis of Physical Maps of Four Bacillus subtilis (natto) Genomes

Qiu, Dongru, Fujita, Kyoko, Sakuma, Yuko, Tanaka, Teruo, Ohashi, Yoshiaki, Ohshima, Hideyuki, ...

The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome...

Cleavage of double-stranded RNA by RNase HI from a thermoacidophilic archaeon, Sulfolobus tokodaii 7

Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro

ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and...

Identification of the first archaeal Type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI can cleave an RNA–DNA junction

Ohtani, Naoto, Yanagawa, Hiroshi, Tomita, Masaru, Itaya, Mitsuhiro

All the archaeal genomes sequenced to date contain a single Type 2 RNase H gene. We found that the genome of a halophilic archaeon, Halobacterium sp. NRC-1, contains an open reading frame with...

Computational comparative analyses of alternative splicing regulation using full-length cDNA of various eukaryotes

ITOH, HITOMI, WASHIO, TAKANORI, TOMITA, MASARU

We previously reported a computational approach to infer alternative splicing patterns from Mus musculus full-length cDNA clones and microarray data. Although we predicted a large number of...

Archaeal Pyrococcus furiosus thymidylate synthase 1 is an RNA-binding protein

Kanai, Akio, Sato, Asako, Imoto, Jun, Tomita, Masaru

Using a stem–loop RNA oligonucleotide (19-mer) containing an AUG sequence in the loop region as a probe, we screened the protein library from a hyperthermophilic archaeon, Pyrococcus furiosus, and...

Large-scale identification of protein–protein interaction of Escherichia coli K-12

Arifuzzaman, Mohammad, Maeda, Maki, Itoh, Aya, Nishikata, Kensaku, Takita, Chiharu, Saito, Rintaro, ...

Protein–protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular...

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

Baba, Tomoya, Ara, Takeshi, Hasegawa, Miki, Takai, Yuki, Okumura, Yoshiko, Baba, Miki, ...

We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin...

Role of Phosphoenolpyruvate in the NADP-Isocitrate Dehydrogenase and Isocitrate Lyase Reaction in Escherichia coli▿

Ogawa, Tadashi, Murakami, Keiko, Mori, Hirotada, Ishii, Nobuyoshi, Tomita, Masaru, Yoshin, Masataka

Phosphoenolpyruvate inhibited Escherichia coli NADP-isocitrate dehydrogenase allosterically (Ki of 0.31 mM) and isocitrate lyase uncompetitively (Ki′ of 0.893 mM). Phosphoenolpyruvate enhances the...

Modeling Hsp70-Mediated Protein Folding

Hu, Bin, Mayer, Matthias P., Tomita, Masaru

The Hsp70 chaperone system is the major molecular chaperone system that assists protein-folding processes in all cells. To understand these processes, we analyzed the kinetic characteristics of the...

In silico screening of archaeal tRNA-encoding genes having multiple introns with bulge-helix-bulge splicing motifs

Sugahara, Junichi, Yachie, Nozomu, Arakawa, Kazuharu, Tomita, Masaru

In archaeal species, several transfer RNA genes have been reported to contain endogenous introns. Although most of the introns are located at anticodon loop regions between nucleotide positions 37...

Sequence Evidence in the Archaeal Genomes that tRNAs Emerged Through the Combination of Ancestral Genes as 5′ and 3′ tRNA Halves

Fujishima, Kosuke, Sugahara, Junichi, Tomita, Masaru, Kanai, Akio

The discovery of separate 5′ and 3′ halves of transfer RNA (tRNA) molecules—so-called split tRNA—in the archaeal parasite Nanoarchaeum equitans made us wonder whether ancestral tRNA was...

Variation in the Correlation of G + C Composition with Synonymous Codon Usage Bias among Bacteria

Suzuki, Haruo, Saito, Rintaro, Tomita, Masaru

G + C composition at the third codon position (GC3) is widely reported to be correlated with synonymous codon usage bias. However, no quantitative attempt has been made to compare the extent of this...

Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis

Sugiyama, Naoyuki, Nakagami, Hirofumi, Mochida, Keiichi, Daudi, Arsalan, Tomita, Masaru, Shirasu, Ken, ...

Protein phosphorylation regulates a wide range of cellular processes. Here, we report the proteome-wide mapping of in vivo phosphorylation sites in Arabidopsis by using complementary phosphopeptide...

Development of Bottom-Fermenting Saccharomyces Strains That Produce High SO2 Levels, Using Integrated Metabolome and Transcriptome Analysis▿

Yoshida, Satoshi, Imoto, Jun, Minato, Toshiko, Oouchi, Rie, Sugihara, Mao, Imai, Takeo, ...

Sulfite plays an important role in beer flavor stability. Although breeding of bottom-fermenting Saccharomyces strains that produce high levels of SO2 is desirable, it is complicated by the fact that...

Simulation of developmental changes in action potentials with ventricular cell models

Itoh, Hitomi, Naito, Yasuhiro, Tomita, Masaru

During cardiomyocyte development, early embryonic ventricular cells show spontaneous activity that disappears at a later stage. Dramatic changes in action potential are mediated by developmental...

The Hsp70 chaperone system maintains high concentrations of active proteins and suppresses ATP consumption during heat shock

Hu, Bin, Tomita, Masaru

Hsp70 chaperones assist protein folding by cycling between the ATP-bound T state with low affinity for substrates and the ADP-bound R state with high affinity for substrates. The transition from the...

Signaling Flux Redistribution at Toll-Like Receptor Pathway Junctions

Selvarajoo, Kumar, Takada, Yasunari, Gohda, Jin, Helmy, Mohamed, Akira, Shizuo, Tomita, Masaru, ...

Various receptors on cell surface recognize specific extracellular molecules and trigger signal transduction altering gene expression in the nucleus. Gain or loss-of-function mutations of one...

Predicting Novel Features of Toll-Like Receptor 3 Signaling in Macrophages

Helmy, Mohamed, Gohda, Jin, Inoue, Jun-ichiro, Tomita, Masaru, Tsuchiya, Masa, Selvarajoo, Kumar

The Toll-like receptor (TLR) 3 plays a critical role in mammalian innate immune response against viral attacks by recognizing double-stranded RNA (dsRNA) or its synthetic analog...

Naoko Watanabe

Riou Matsushima, Akiko Kawase, Hisako Nakano, Kanako Saito, Masaru Tomita

In this work, we try to model and simulate human red blood cell using E-CELL system, a generic software for simulation of cellular processes [1]. Human red blood cell has been well-studied in last...

On Low Frequency of CpG Dinucleotides in Bacterial Genomes Mami Goto

Takanori Washio, Masaru Tomita

CpG depletion is a phenomenon known to be caused by CpG DNA methylation. Although CpG DNA methylation is believed to be a feature of vertebrates and plants [1], we have found that some procaryote...

Naoko Watanabe

Modeling Human, Red Blood Cell, Riou Matsushima, Akiko Kawase, Hisako Nakano, Kanako Saito, ...

In this work, we try to model and simulate human red blood cell using E-CELL system, a generic software for simulation of cellular processes [1]. Human red blood cell has been well-studied in last...

Emergent Genome-Wide Control in Wildtype and Genetically Mutated Lipopolysaccarides-Stimulated Macrophages

Tsuchiya, Masa, Piras, Vincent, Choi, Sangdun, Akira, Shizuo, Tomita, Masaru, Giuliani, Alessandro, ...

Large-scale gene expression studies have mainly focused on highly expressed and ‘discriminatory’ genes to decipher key regulatory processes. Biological responses are consequence of the concerted...

Stabilizing synthetic data in the DNA of living organisms

Yachie, Nozomu, Ohashi, Yoshiaki, Tomita, Masaru

Data-encoding synthetic DNA, inserted into the genome of a living organism, is thought to be more robust than the current media. Because the living genome is duplicated and copied into new...

Selection Effects on the Positioning of Genes and Gene Structures from the Interplay of Replication and Transcription in Bacterial Genomes

Arakawa, Kazuharu, Tomita, Masaru

Bacterial chromosomes are partly shaped by the functional requirements for efficient replication, which lead to strand bias as commonly characterized by the excess of guanines over cytosines in the...

The GC Skew Index: A Measure of Genomic Compositional Asymmetry and the Degree of Replicational Selection

Arakawa, Kazuharu, Tomita, Masaru

Circular bacterial chromosomes have highly polarized nucleotide composition in the two replichores, and this genomic strand asymmetry can be visualized using GC skew graphs. Here we propose and...

Network Features and Pathway Analyses of a Signal Transduction Cascade

Yanashima, Ryoji, Kitagawa, Noriyuki, Matsubara, Yoshiya, Weatheritt, Robert, Oka, Kotaro, Kikuchi, Shinichi, ...

The scale-free and small-world network models reflect the functional units of networks. However, when we investigated the network properties of a signaling pathway using these models, no significant...

Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs

Kosugi, Shunichi, Hasebe, Masako, Tomita, Masaru, Yanagawa, Hiroshi

The cell cycle-dependent nucleocytoplasmic transport of proteins is predominantly regulated by CDK kinase activities; however, it is currently difficult to predict the proteins thus regulated,...

Tri-split tRNA is a transfer RNA made from 3 transcripts that provides insight into the evolution of fragmented tRNAs in archaea

Fujishima, Kosuke, Sugahara, Junichi, Kikuta, Kaoru, Hirano, Reiko, Sato, Asako, Tomita, Masaru, ...

Transfer RNA (tRNA) is essential for decoding the genome sequence into proteins. In Archaea, previous studies have revealed unique multiple intron-containing tRNAs and tRNAs that are encoded on 2...

Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus

Kanai, Akio, Sato, Asako, Fukuda, Yoko, Okada, Kiyoshi, Matsuda, Takashi, Sakamoto, Taiichi, ...

Using an expression protein library of a hyperthermophilic archaeon, Pyrococcus furiosus, we identified a gene (PF0027) that encodes a protein with heat-stable cyclic nucleotide phosphodiesterase...

A web server for interactive and zoomable Chaos Game Representation images

Arakawa, Kazuharu, Oshita, Kazuki, Tomita, Masaru

Chaos Game Representation (CGR) is a generalized scale-independent Markov transition table, which is useful for the visualization and comparative study of genomic signature, or for the study of...

Systematic phenome analysis of Escherichia coli multiple-knockout mutants reveals hidden reactions in central carbon metabolism

Nakahigashi, Kenji, Toya, Yoshihiro, Ishii, Nobuyoshi, Soga, Tomoyoshi, Hasegawa, Miki, Watanabe, Hisami, ...

Central carbon metabolism is a basic and exhaustively analyzed pathway. However, the intrinsic robustness of the pathway might still conceal uncharacterized reactions. To test this hypothesis, we...

Proteome-Wide Prediction of Novel DNA/RNA-Binding Proteins Using Amino Acid Composition and Periodicity in the Hyperthermophilic Archaeon Pyrococcus furiosus

Fujishima, Kosuke, Komasa, Mizuki, Kitamura, Sayaka, Suzuki, Haruo, Tomita, Masaru, Kanai, Akio

Proteins play a critical role in complex biological systems, yet about half of the proteins in publicly available databases are annotated as functionally unknown. Proteome-wide functional...