Masataka Tsuda

GenomeMatcher: A graphical user interface for DNA sequence comparison (2008)

Ohtsubo, Yoshiyuki, Ikeda-Ohtsubo, Wakako, Nagata, Yuji, Tsuda, Masataka

Abstract Background The number of available genome sequences is increasing, and easy-to-use software that enables efficient comparative analysis is needed. Results We developed GenomeMatcher, a...

Construction of Signature-tagged Mutant Library in Mesorhizobium loti as a Powerful Tool for Functional Genomics (2008)

Shimoda, Yoshikazu, Mitsui, Hisayuki, Kamimatsuse, Hiroko, Minamisawa, Kiwamu, Nishiyama, Eri, Ohtsubo, Yoshiyuki, ...

Rhizobia are nitrogen-fixing soil bacteria that establish endosymbiosis with some leguminous plants. The completion of several rhizobial genome sequences provides opportunities for genome-wide...

Site-Specific Recombination System Encoded by Toluene Catabolic (2002)

Transposon Tn, Hiroyuki Genka, Yuji Nagata, Masataka Tsuda

The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site,...

Identification and Characterization of Tn4656, a Novel Class II Transposon Carrying a Set of Toluene-Degrading Genes from TOL Plasmid pWW53

Tsuda, Masataka, Genka, Hiroyuki

It has been reported that the toluene-degrading (xyl) genes from Pseudomonas putida plasmid pWW53 are able to translocate to broad-host-range drug resistance plasmid RP4, and pWW53-4 is one of the...

Characterization of the MexC-MexD-OprJ Multidrug Efflux System in ΔmexA-mexB-oprM Mutants of Pseudomonas aeruginosa

Gotoh, Naomasa, Tsujimoto, Hideto, Tsuda, Masataka, Okamoto, Kiyomi, Nomura, Atsuko, Wada, Takaomi, ...

Expression of the multidrug efflux system MexC-MexD-OprJ in nfxB mutants of Pseudomonas aeruginosa contributes to resistance to fluoroquinolones and the “fourth-generation” cephems (cefpirome and...

Nucleotide Sequence and Characterization of cdrA, a Cell Division-Related Gene of Helicobacter pylori

Takeuchi, Hiroaki, Shirai, Mutsunori, Akada, Junko K., Tsuda, Masataka, Nakazawa, Teruko

We identified cell division-related gene cdrA in Helicobacter pylori HPK5. The putative gene product, CdrA, is a 367-amino-acid polypeptide that exhibited a high level of homology to conserved...

Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1

Sota, Masahiro, Endo, Masahiro, Nitta, Keiji, Kawasaki, Haruhiko, Tsuda, Masataka

The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on...

Site-Specific Recombination System Encoded by Toluene Catabolic Transposon Tn4651

Genka, Hiroyuki, Nagata, Yuji, Tsuda, Masataka

The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site,...

Reconstruction of Mycobacterial Dehalogenase Rv2579 by Cumulative Mutagenesis of Haloalkane Dehalogenase LinB

Nagata, Yuji, Prokop, Zbyněk, Marvanová, Soňa, Sýkorová, Jana, Monincová, Marta, Tsuda, Masataka, ...

The homology model of protein Rv2579 from Mycobacterium tuberculosis H37Rv was compared with the crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26, and this...

Structure of Haloacetate-Catabolic IncP-1β Plasmid pUO1 and Genetic Mobility of Its Residing Haloacetate-Catabolic Transposon

Sota, Masahiro, Kawasaki, Haruhiko, Tsuda, Masataka

The self-transmissible plasmid pUO1 from Delftia acidovorans strain B carries two haloacetate-catabolic transposons, TnHad1 and TnHad2, and the mer genes for resistance to mercury. The complete...

Identification and Characterization of Genes Involved in the Downstream Degradation Pathway of γ-Hexachlorocyclohexane in Sphingomonas paucimobilis UT26

Endo, Ryo, Kamakura, Mayuko, Miyauchi, Keisuke, Fukuda, Masao, Ohtsubo, Yoshiyuki, Tsuda, Masataka, ...

Sphingomonas paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. In our previous study, we cloned and characterized genes that are involved in the...

High-Temperature-Induced Transposition of Insertion Elements in Burkholderia multivorans ATCC 17616

Ohtsubo, Yoshiyuki, Genka, Hiroyuki, Komatsu, Harunobu, Nagata, Yuji, Tsuda, Masataka

An efficient and quantitative method to analyze the transposition of various insertion sequence (IS) elements in Burkholderia multivorans ATCC 17616 was devised. pGEN500, a plasmid carrying a...

Degradation of β-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26

Nagata, Yuji, Prokop, Zbyněk, Sato, Yukari, Jerabek, Petr, Kumar, Ashwani, Ohtsubo, Yoshiyuki, ...

β-Hexachlorocyclohexane (β-HCH) is the most recalcitrant among the α-, β-, γ-, and δ-isomers of HCH and causes serious environmental pollution problems. We demonstrate here that the haloalkane...

Two Rhizobial Strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, Encode Haloalkane Dehalogenases with Novel Structures and Substrate Specificities

Sato, Yukari, Monincová, Marta, Chaloupková, Radka, Prokop, Zbyňek, Ohtsubo, Yoshiyuki, Minamisawa, Kiwamu, ...

Haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants. Two rhizobial strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, have open...

Functional Analysis of Unique Class II Insertion Sequence IS1071

Sota, Masahiro, Yano, Hirokazu, Nagata, Yuji, Ohtsubo, Yoshiyuki, Genka, Hiroyuki, Anbutsu, Hisashi, ...

Various xenobiotic-degrading genes on many catabolic plasmids are often flanked by two copies of an insertion sequence, IS1071. This 3.2-kb IS element has long (110-bp) terminal inverted repeats...

Distribution and Organization of Auxotrophic Genes on the Multichromosomal Genome of Burkholderia multivorans ATCC 17616

Komatsu, Harunobu, Imura, Yoshiyuki, Ohori, Akira, Nagata, Yuji, Tsuda, Masataka

The Burkholderia multivorans strain ATCC 17616 carries three circular chromosomes with sizes of 3.4, 2.5, and 0.9 Mb. To determine the distribution and organization of the amino acid biosynthetic...

Characterization of the Replication, Maintenance, and Transfer Features of the IncP-7 Plasmid pCAR1, Which Carries Genes Involved in Carbazole and Dioxin Degradation†

Shintani, Masaki, Yano, Hirokazu, Habe, Hiroshi, Omori, Toshio, Yamane, Hisakazu, Tsuda, Masataka, ...

Isolated from Pseudomonas resinovorans CA10, pCAR1 is a 199-kb plasmid that carries genes involved in the degradation of carbazole and dioxin. The nucleotide sequence of pCAR1 has been determined...

Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase†

Sota, Masahiro, Yano, Hirokazu, Ono, Akira, Miyazaki, Ryo, Ishii, Hidenori, Genka, Hiroyuki, ...

The naphthalene-catabolic (nah) genes on the incompatibility group P-9 (IncP-9) self-transmissible plasmid NAH7 from Pseudomonas putida G7 are some of the most extensively characterized genetic...

Identification and Characterization of Tn4656, a Novel Class II Transposon Carrying a Set of Toluene-Degrading Genes from TOL Plasmid pWW53

Tsuda, Masataka, Genka, Hiroyuki

It has been reported that the toluene-degrading (xyl) genes from Pseudomonas putida plasmid pWW53 are able to translocate to broad-host-range drug resistance plasmid RP4, and pWW53-4 is one of the...

Characterization of the MexC-MexD-OprJ Multidrug Efflux System in ΔmexA-mexB-oprM Mutants of Pseudomonas aeruginosa

Gotoh, Naomasa, Tsujimoto, Hideto, Tsuda, Masataka, Okamoto, Kiyomi, Nomura, Atsuko, Wada, Takaomi, ...

Expression of the multidrug efflux system MexC-MexD-OprJ in nfxB mutants of Pseudomonas aeruginosa contributes to resistance to fluoroquinolones and the “fourth-generation” cephems (cefpirome and...

Nucleotide Sequence and Characterization of cdrA, a Cell Division-Related Gene of Helicobacter pylori

Takeuchi, Hiroaki, Shirai, Mutsunori, Akada, Junko K., Tsuda, Masataka, Nakazawa, Teruko

We identified cell division-related gene cdrA in Helicobacter pylori HPK5. The putative gene product, CdrA, is a 367-amino-acid polypeptide that exhibited a high level of homology to conserved...

Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1

Sota, Masahiro, Endo, Masahiro, Nitta, Keiji, Kawasaki, Haruhiko, Tsuda, Masataka

The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on...

Site-Specific Recombination System Encoded by Toluene Catabolic Transposon Tn4651

Genka, Hiroyuki, Nagata, Yuji, Tsuda, Masataka

The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site,...

Reconstruction of Mycobacterial Dehalogenase Rv2579 by Cumulative Mutagenesis of Haloalkane Dehalogenase LinB

Nagata, Yuji, Prokop, Zbyněk, Marvanová, Soňa, Sýkorová, Jana, Monincová, Marta, Tsuda, Masataka, ...

The homology model of protein Rv2579 from Mycobacterium tuberculosis H37Rv was compared with the crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26, and this...

Distribution and Organization of Auxotrophic Genes on the Multichromosomal Genome of Burkholderia multivorans ATCC 17616

Komatsu, Harunobu, Imura, Yoshiyuki, Ohori, Akira, Nagata, Yuji, Tsuda, Masataka

The Burkholderia multivorans strain ATCC 17616 carries three circular chromosomes with sizes of 3.4, 2.5, and 0.9 Mb. To determine the distribution and organization of the amino acid biosynthetic...

Structure of Haloacetate-Catabolic IncP-1β Plasmid pUO1 and Genetic Mobility of Its Residing Haloacetate-Catabolic Transposon

Sota, Masahiro, Kawasaki, Haruhiko, Tsuda, Masataka

The self-transmissible plasmid pUO1 from Delftia acidovorans strain B carries two haloacetate-catabolic transposons, TnHad1 and TnHad2, and the mer genes for resistance to mercury. The complete...

Identification and Characterization of Genes Involved in the Downstream Degradation Pathway of γ-Hexachlorocyclohexane in Sphingomonas paucimobilis UT26

Endo, Ryo, Kamakura, Mayuko, Miyauchi, Keisuke, Fukuda, Masao, Ohtsubo, Yoshiyuki, Tsuda, Masataka, ...

Sphingomonas paucimobilis UT26 utilizes γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. In our previous study, we cloned and characterized genes that are involved in the...

High-Temperature-Induced Transposition of Insertion Elements in Burkholderia multivorans ATCC 17616

Ohtsubo, Yoshiyuki, Genka, Hiroyuki, Komatsu, Harunobu, Nagata, Yuji, Tsuda, Masataka

An efficient and quantitative method to analyze the transposition of various insertion sequence (IS) elements in Burkholderia multivorans ATCC 17616 was devised. pGEN500, a plasmid carrying a...

Degradation of β-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Sphingomonas paucimobilis UT26

Nagata, Yuji, Prokop, Zbyněk, Sato, Yukari, Jerabek, Petr, Kumar, Ashwani, Ohtsubo, Yoshiyuki, ...

β-Hexachlorocyclohexane (β-HCH) is the most recalcitrant among the α-, β-, γ-, and δ-isomers of HCH and causes serious environmental pollution problems. We demonstrate here that the haloalkane...

Two Rhizobial Strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, Encode Haloalkane Dehalogenases with Novel Structures and Substrate Specificities

Sato, Yukari, Monincová, Marta, Chaloupková, Radka, Prokop, Zbyňek, Ohtsubo, Yoshiyuki, Minamisawa, Kiwamu, ...

Haloalkane dehalogenases are key enzymes for the degradation of halogenated aliphatic pollutants. Two rhizobial strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, have open...

Functional Analysis of Unique Class II Insertion Sequence IS1071

Sota, Masahiro, Yano, Hirokazu, Nagata, Yuji, Ohtsubo, Yoshiyuki, Genka, Hiroyuki, Anbutsu, Hisashi, ...

Various xenobiotic-degrading genes on many catabolic plasmids are often flanked by two copies of an insertion sequence, IS1071. This 3.2-kb IS element has long (110-bp) terminal inverted repeats...

Characterization of the Replication, Maintenance, and Transfer Features of the IncP-7 Plasmid pCAR1, Which Carries Genes Involved in Carbazole and Dioxin Degradation†

Shintani, Masaki, Yano, Hirokazu, Habe, Hiroshi, Omori, Toshio, Yamane, Hisakazu, Tsuda, Masataka, ...

Isolated from Pseudomonas resinovorans CA10, pCAR1 is a 199-kb plasmid that carries genes involved in the degradation of carbazole and dioxin. The nucleotide sequence of pCAR1 has been determined...

Genomic and Functional Analysis of the IncP-9 Naphthalene-Catabolic Plasmid NAH7 and Its Transposon Tn4655 Suggests Catabolic Gene Spread by a Tyrosine Recombinase†

Sota, Masahiro, Yano, Hirokazu, Ono, Akira, Miyazaki, Ryo, Ishii, Hidenori, Genka, Hiroyuki, ...

The naphthalene-catabolic (nah) genes on the incompatibility group P-9 (IncP-9) self-transmissible plasmid NAH7 from Pseudomonas putida G7 are some of the most extensively characterized genetic...

Complete Nucleotide Sequence of an Exogenously Isolated Plasmid, pLB1, Involved in γ-Hexachlorocyclohexane Degradation▿

Miyazaki, Ryo, Sato, Yukari, Ito, Michihiro, Ohtsubo, Yoshiyuki, Nagata, Yuji, Tsuda, Masataka

The α-proteobacterial strain Sphingobium japonicum UT26 utilizes a highly chlorinated pesticide, γ-hexachlorocyclohexane (γ-HCH), as a sole source of carbon and energy, and haloalkane dehalogenase...

Region-Specific Insertion of Transposons in Combination with Selection for High Plasmid Transferability and Stability Accounts for the Structural Similarity of IncP-1 Plasmids▿

Sota, Masahiro, Tsuda, Masataka, Yano, Hirokazu, Suzuki, Haruo, Forney, Larry J., Top, Eva M.

The overall architecture of IncP-1 plasmids is very conserved in that the accessory genes are typically located in one or two specific regions: between oriV and trfA and between the tra and trb...

Identification and Characterization of Genes Encoding a Putative ABC-Type Transporter Essential for Utilization of γ-Hexachlorocyclohexane in Sphingobium japonicum UT26▿ †

Endo, Ryo, Ohtsubo, Yoshiyuki, Tsuda, Masataka, Nagata, Yuji

Sphingobium japonicum UT26 utilizes γ-hexachlorocyclohexane (γ-HCH) as its sole source of carbon and energy. In our previous studies, we cloned and characterized genes encoding enzymes for the...

Crystallization and preliminary crystallographic analysis of a haloalkane dehalogenase, DbjA, from Bradyrhizobium japonicum USDA110

Sato, Yukari, Natsume, Ryo, Tsuda, Masataka, Damborsky, Jiri, Nagata, Yuji, Senda, Toshiya

A haloalkane dehalogenase, DbjA, was crystallized by the hanging-drop vapour-diffusion method using PEG 4000 as a precipitant. The crystal belongs to the orthorhombic system, space group P21212 and...

Characterization of the traD Operon of Naphthalene-Catabolic Plasmid NAH7: a Host-Range Modifier in Conjugative Transfer▿ †

Miyazaki, Ryo, Ohtsubo, Yoshiyuki, Nagata, Yuji, Tsuda, Masataka

Pseudomonas putida G7 carries a naphthalene-catabolic and self-transmissible plasmid, NAH7, which belongs to the IncP-9 incompatibility group. Adjacent to the putative origin of conjugative transfer...

Construction of Signature-tagged Mutant Library in Mesorhizobium loti as a Powerful Tool for Functional Genomics

Shimoda, Yoshikazu, Mitsui, Hisayuki, Kamimatsuse, Hiroko, Minamisawa, Kiwamu, Nishiyama, Eri, Ohtsubo, Yoshiyuki, ...

Rhizobia are nitrogen-fixing soil bacteria that establish endosymbiosis with some leguminous plants. The completion of several rhizobial genome sequences provides opportunities for genome-wide...