Masato Ohtsuka

Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector (2009)

Masato Ohtsuka, Takayuki Warita, Takayuki Sakurai, Satoshi Watanabe, Hidetoshi Inoko, Masahiro Sato

We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red...

Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector (2009)

Masato Ohtsuka, Takayuki Warita, Takayuki Sakurai, Satoshi Watanabe, Hidetoshi Inoko, Masahiro Sato

We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red...

Major histocompatibility complex(Mhc) class Ib gene duplications, organization and expression patterns in mouse strain C57BL/6 (2008)

Ohtsuka, Masato, Inoko, Hidetoshi, Kulski, Jerzy K, Yoshimura, Shinichi

Abstract Background The mouse has more than 30 Major histocompatibility complex ( Mhc ) class Ib genes, most of which exist in the H2 region of chromosome 17 in distinct gene clusters. Although...

An EYFP insertion mutant containing a modified lox sequence for potential use as a recombination indicator (2005)

Mizutani, Akiko, Ohtsuka, Masato, Kimura, Minoru, Tanaka, Masafumi, Inoko, Hidetoshi

The Cre-loxP system has been used for genetic engineering in many organisms ranging from E. coli to mouse. It is known that intermolecular recombination between two loxP sites is substantially less...

CHOP: visualization of 'wobbling' and isolation of highly conserved regions from aligned DNA sequences (2004)

Ohtsuka, Masato, Horiuchi, Shohei, Kulski, Jerzy K., Kimura, Minoru, Inoko, Hidetoshi

The web software CHOP was developed to visualize the ‘wobbling’ in the third codon position of aligned DNA sequences. The simple features of this tool allow users to easily find regions suspected...

Direct subcloning of target region from BAC insert using restriction enzymes that produce non-identical cohesive ends (2004)

Ohmi, Yuhsuke, Sato, Masahiro, Ohtsuka, Masato

Type IIS or interrupted palindrome restriction endonuclease digestion produces unique cohesive ends. Utilization of this feature of BglI, SfiI and BstXI has led to direct subcloning of 28.5-kb and...

Repeated GenomiPhi, phi29 DNA polymerase-based rolling circle amplification, is useful for generation of large amounts of plasmid DNA (2004)

Sato, Masahiro, Ohtsuka, Masato, Ohmi, Yuhsuke

The GenomiPhi™ DNA Amplification Kit (Amersham Biosciences Co., Piscataway, NJ, USA) uses a novel, isothermal DNA amplification technique for production of microgram quantities of DNA from...

Isolation and characterization of three genes paralogous to mouse Ring3 (2001)

Taniguchi, Yasushi, Suzuki, Hiroyuki, Ohtsuka, Masato, Kikuchi, Natsuko, Kimura, Minoru, Inoko, Hidetoshi

Isolation and characterization of three genes paralogous to mouse Ring3 (2001)

Taniguchi, Yasushi, Suzuki, Hiroyuki, Ohtsuka, Masato, Kikuchi, Natsuko, Kimura, Minoru, Inoko, Hidetoshi

Syntenic chromosomal areas share paralogous genes which are believed to have been generated by repeated duplication of an ancestral gene. The human RING3 gene is known to have paralogous...

Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning (1999)

Ohtsuka, Masato, Makino, Satoshi, Yoda, Kinya, Wada, Hironori, Naruse, Kiyoshi, Mitani, Hiroshi, ...

Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning

Ohtsuka, Masato, Makino, Satoshi, Yoda, Kinya, Wada, Hironori, Naruse, Kiyoshi, Mitani, Hiroshi, ...

Double anal fin (Da) is a medaka with an autosomal semidominant mutation that causes mirror image duplication of the ventral region concentrating on the caudal region. The chromosomal location of the...

CHOP: visualization of ‘wobbling’ and isolation of highly conserved regions from aligned DNA sequences

Ohtsuka, Masato, Horiuchi, Shohei, Kulski, Jerzy K., Kimura, Minoru, Inoko, Hidetoshi

The web software CHOP was developed to visualize the ‘wobbling’ in the third codon position of aligned DNA sequences. The simple features of this tool allow users to easily find regions suspected...

Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning

Ohtsuka, Masato, Makino, Satoshi, Yoda, Kinya, Wada, Hironori, Naruse, Kiyoshi, Mitani, Hiroshi, ...

Double anal fin (Da) is a medaka with an autosomal semidominant mutation that causes mirror image duplication of the ventral region concentrating on the caudal region. The chromosomal location of the...

CHOP: visualization of ‘wobbling’ and isolation of highly conserved regions from aligned DNA sequences

Ohtsuka, Masato, Horiuchi, Shohei, Kulski, Jerzy K., Kimura, Minoru, Inoko, Hidetoshi

The web software CHOP was developed to visualize the ‘wobbling’ in the third codon position of aligned DNA sequences. The simple features of this tool allow users to easily find regions suspected...

Major histocompatibility complex (Mhc) class Ib gene duplications, organization and expression patterns in mouse strain C57BL/6

Ohtsuka, Masato, Inoko, Hidetoshi, Kulski, Jerzy K, Yoshimura, Shinichi

Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector

Ohtsuka, Masato, Warita, Takayuki, Sakurai, Takayuki, Watanabe, Satoshi, Inoko, Hidetoshi, Sato, Masahiro

We developed Cre-loxP-based systems, termed CRTEIL and CETRIZ, which allow gene switching in a noninvasive manner. Single transfection with pCRTEIL resulted in predominant expression of red...