Kawachi, Miki, Kobae, Yoshihiro, Mori, Haruki, Tomioka, Rie, Lee, Youngsook, Maeshima, Masayoshi
A mutant line of Arabidopsis thaliana that lacks a vacuolar membrane Zn2+/H+ antiporter MTP1 is sensitive to zinc. We examined the physiological changes in this loss-of-function mutant under high-Zn...
Queirós, Filipa, Fontes, Natacha, Silva, Paulo, Almeida, Domingos, Maeshima, Masayoshi, Gerós, Hernâni, ...
The efficient exclusion of excess Na from the cytoplasm and vacuolar Na+ accumulation are the main mechanisms for the adaptation of plants to salt stress. This is typically carried out by...
Hirono, Megumi, Maeshima, Masayoshi
H+-translocating pyrophosphatase converts energy from hydrolysis of pyrophosphate to active H+ transport across biomembranes. Mutational analysis of Streptomyces coelicolor A3(2) enzyme revealed that...
Hamamoto, Shin, Marui, Junichiro, Matsuoka, Ken, Higashi, Kyohei, Igarashi, Kazuei, Nakagawa, Tsuyoshi, ...
Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities (2008)
Sakurai, Junko, Ahamed, Arifa, Murai, Mari, Maeshima, Masayoshi, Uemura, Matsuo
Water transport in plants is greatly dependent on the expression and activity of water transport channels, called aquaporins. Here, we have clarified the tissue- and cell-specific localization of...
Murai-Hatano, Mari, Kuwagata, Tsuneo, Sakurai, Junko, Nonami, Hiroshi, Ahamed, Arifa, Nagasuga, Kiyoshi, ...
The role of root temperature TR in regulating the water-uptake capability of rice roots and the possible relationship with aquaporins were investigated. The root hydraulic conductivity Lpr decreased...
Nagasaki-Takeuchi, Nahoko, Miyano, Masashi, Maeshima, Masayoshi
PCaP1, a hydrophilic cation-binding protein, is bound to the plasma membrane in Arabidopsis thaliana. We focused on the physicochemical properties of PCaP1 to understand its uniqueness in terms of...
Ishijima, Jun, Nagasaki, Nahoko, Maeshima, Masayoshi, Miyano, Masashi
A unique acidic calcium-binding protein RVCaB, rich in glutamic acid and proline and lacking aromatic amino-acid residues, exists in radish vacuoles, and is thought to be involved in the vacuole...
Ide, Yuki, Nagasaki, Nahoko, Tomioka, Rie, Suito, Momoe, Kamiya, Takehiro, Maeshima, Masayoshi
A new type of protein was found in Arabidopsis thaliana, PCaP1, which is rich in glutamate and lysine residues. The protein bound 45Ca2+ even in the presence of a high concentration of Mg2+....
Ide, Yuki, Tomioka, Rie, Ouchi, Yuya, Kamiya, Takehiro, Maeshima, Masayoshi
Ca2+-signaling in downstream effectors is supported by many kinds of Ca2+-binding proteins, which function as a signal mediator and a Ca2+-buffering protein. We found in Arabidopsis thaliana a new...
Vacuolar transporters and their essential role in plant metabolism (2007)
Martinoia, Enrico, Maeshima, Masayoshi, Neuhaus, H. Ekkehard
Following the unequivocal demonstration that plants contain at least two types of vacuoles, scientists studying this organelle have realized that the plant ‘vacuome’ is far more complex than they...
Ide, Yuki, Nagasaki, Nahoko, Tomioka, Rie, Suito, Momoe, Kamiya, Takehiro, Maeshima, Masayoshi
A new type of protein was found in Arabidopsis thaliana, PCaP1, which is rich in glutamate and lysine residues. The protein bound 45Ca2+ even in the presence of a high concentration of Mg2+....
Kobae, Yoshihiro, Sekino, Tetsuro, Yoshioka, Hirofumi, Nakagawa, Tsuyoshi, Martinoia, Enrico, Maeshima, Masayoshi
Plants contain a large number of ATP-binding cassette (ABC) transporters belonging to different subclasses. AtPDR8 is the only member of the pleiotropic drug resistance (PDR) ABC transporter subclass...
Mizutani, Masahiro, Watanabe, Satoshi, Nakagawa, Tsuyoshi, Maeshima, Masayoshi
We investigated a nodulin 26-like protein NIP2;1, which belongs to the third subgroup of Arabidopsis aquaporins. Histochemical analysis of a promoter–β-glucuronidase fusion revealed the...
Kobayashi, Michie, Kawakita, Kazuhito, Maeshima, Masayoshi, Doke, Noriyuki, Yoshioka, Hirofumi
Rapid generation of reactive oxygen species (ROS) at the cell surface has been implicated in plant defence responses. Genetic evidence indicates that a plant NADPH oxidase (Rboh; respiratory burst...
Kamiya, Takehiro, Akahori, Taro, Ashikari, Motoyuki, Maeshima, Masayoshi
Calcium is an essential macronutrient for plants and functions in signal transduction. Regulation of the cytosolic calcium concentration is required for normal cell growth. In calcium homeostasis in...
Kobae, Yoshihiro, Sekino, Tetsuro, Yoshioka, Hirofumi, Nakagawa, Tsuyoshi, Martinoia, Enrico, Maeshima, Masayoshi
Plants contain a large number of ATP-binding cassette (ABC) transporters belonging to different subclasses. AtPDR8 is the only member of the pleiotropic drug resistance ABC transporter (PDR) subclass...
Mizutani, Masahiro, Watanabe, Satoshi, Nakagawa, Tsuyoshi, Maeshima, Masayoshi
We investigated a nodulin 26-like protein NIP2;1, which belongs to the third subgroup of Arabidopsis aquaporins. Histochemical analysis of a promoter-β-glucuronidase fusion revealed the...
Ide, Yuki, Tomioka, Rie, Ouchi, Yuya, Kamiya, Takehiro, Maeshima, Masayoshi
Ca2+-signaling in downstream effectors is supported by many kinds of Ca2+-binding proteins, which function as a signal mediator and a Ca2+-buffering protein. We found in Arabidopsis thaliana a new...
Kobayashi, Michie, Kawakita, Kazuhito, Maeshima, Masayoshi, Doke, Noriyuki, Yoshioka, Hirofumi
Rapid generation of reactive oxygen species (ROS) at the cell surface has been implicated in plant defence responses. Genetic evidence indicates that a plant NADPH oxidase (Rboh; respiratory burst...
Oligomerization of H+-pyrophosphatase and its structural and functional consequences (2005)
Mimura, Hisatoshi, Nakanishi, Yoichi, Maeshima, Masayoshi, 前島, 正義
The H+-pyrophosphatase (H+-PPase) consists of a single polypeptide, containing 16 or 17 transmembrane domains. To determine the higher order oligomeric state of Streptomyces coelicolor H+-PPase, we...
Mimura, Hisatoshi, Nakanishi, Yoichi, Maeshima, Masayoshi, 前島, 正義
Redox control of disulfide-bond formation in the H+-pyrophosphatase of Streptomyces coelicolor was investigated using cysteine mutants expressed in Escherichia coli. The wildtype enzyme, but not a...
Novel type aquaporin SIPs are mainly localized to the ER membrane and (2005)
Ishikawa, Fumiyoshi, Suga, Shinobu, Uemura, Tomohiro, Maeshima, Masayoshi, 前島, 正義
We investigated the fourth subgroup of Arabidopsis aquaporin, small and basic intrinsic proteins (SIPs). When they were expressed in yeast, SIP1;1 and SIP1;2, but not SIP2;1, gave water-channel...
Identification of 33 Rice Aquaporin Genes and Analysis of Their Expression and Function (2005)
Sakurai, Junko, Ishikawa, Fumiyoshi, Yamaguchi, Tomoya, Uemura, Matsuo, Maeshima, Masayoshi
Plant aquaporins form a large protein family including plasma membrane-type (PIPs) and tonoplast-type aquaporins (TIPs), and facilitate osmotic water transport across membranes as a key physiological...
FLEURAT-LESSARD, PIERRETTE, MICHONNEAU, PHILIPPE, MAESHIMA, MASAYOSHI, DREVON, JEAN-JACQUES, SERRAJ, RACHID
• Background and Aims The inner cortical cells (IC-cells) of legume root nodules have been previously shown to regulate the resistance to nodule O2 diffusion by a rapid contraction/expansion...
Anderca, Marinela Ioana, Suga, Shinobu, Furuichi, Takuya, Shimogawara, Kosuke, Maeshima, Masayoshi, Muto, Shoshi
Yoshida, Kumi, Kawachi, Miki, Mori, Mihoko, Maeshima, Masayoshi, Kondo, Maki, Nishimura, Mikio, ...
Petal color of morning glory, Ipomoea tricolor cv. Heavenly blue, changes from purplish red to blue during flower-opening. This color change is caused by an unusual increase in vacuolar pH from 6.6...
Identification of 33 Rice Aquaporin Genes and Analysis of Their Expression and Function (2005)
Sakurai, Junko, Ishikawa, Fumiyoshi, Yamaguchi, Tomoya, Uemura, Matsuo, Maeshima, Masayoshi
Plant aquaporins form a large protein family including plasma-membrane-type (PIP) and tonoplast-type aquaporins (TIP) and facilitate the osmotic water transport across membranes as a key...
Kamiya, Takehiro, Akahori, Taro, Maeshima, Masayoshi
We identified five cation/H+ exchangers (CAX) from rice, and phylogenetically divided them into two clusters. Gene expression and absolute amounts of mRNA in different organs were analyzed by...
Kamiya, Takehiro, Akahori, Taro, Ashikari, Motoyuki, Maeshima, Masayoshi
Calcium is an essential macronutrient for plants and functions in signal transduction. Regulation of the cytosolic calcium concentration is required for normal cell growth. In calcium homeostasis in...
Hirono, Megumi, Mimura, Hisatoshi, Nakanishi, Yoichi, Maeshima, Masayoshi
H+-translocating pyrophosphatases (H+-PPases) are proton pumps that are found in many organisms, including plants, bacteria and protozoa. Streptomyces coelicolor is a soil bacterium that...
FLEURAT-LESSARD, PIERRETTE, MICHONNEAU, PHILIPPE, MAESHIMA, MASAYOSHI, DREVON, JEAN-JACQUES, SERRAJ, RACHID
• Background and Aims The inner cortical cells (IC-cells) of legume root nodules have been previously shown to regulate the resistance to nodule O2 diffusion by a rapid contraction/expansion...
Yoshida, Kumi, Kawachi, Miki, Mori, Mihoko, Maeshima, Masayoshi, Kondo, Maki, Nishimura, Mikio, ...
The petal color of morning glory, Ipomoea tricolor cv. Heavenly Blue, changes from purplish red to blue during flower opening. This color change is caused by an unusual increase in vacuolar pH from...
Kamiya, Takehiro, Akahori, Taro, Maeshima, Masayoshi
We identified five cation/H+ exchangers (CAX) from rice, and phylogenetically divided them into two clusters. Gene expression and absolute amounts of mRNA in different organs were analyzed by...
Yoshida, Kumi, Kawachi, Miki, Mori, Mihoko, Maeshima, Masayoshi, Kondo, Maki, Nishimura, Mikio, ...
Petal color of morning glory, Ipomoea tricolor cv. Heavenly blue, changes from purplish red to blue during flower-opening. This color change is caused by an unusual increase in vacuolar pH from 6.6...
Identification of 33 Rice Aquaporin Genes and Analysis of Their Expression and Function (2005)
Sakurai, Junko, Ishikawa, Fumiyoshi, Yamaguchi, Tomoya, Uemura, Matsuo, Maeshima, Masayoshi
Plant aquaporins form a large protein family including plasma-membrane-type (PIP) and tonoplast-type aquaporins (TIP) and facilitate the osmotic water transport across membranes as a key...
Kamiya, Takehiro, Akahori, Taro, Maeshima, Masayoshi
We identified five cation/H+ exchangers (CAX) from rice, and phylogenetically divided them into two clusters. Gene expression and absolute amounts of mRNA in different organs were analyzed by...
Kamiya, Takehiro, Akahori, Taro, Ashikari, Motoyuki, Maeshima, Masayoshi
Calcium is an essential macronutrient for plants and functions in signal transduction. Regulation of the cytosolic calcium concentration is required for normal cell growth. In calcium homeostasis in...
FLEURAT-LESSARD, PIERRETTE, MICHONNEAU, PHILIPPE, MAESHIMA, MASAYOSHI, DREVON, JEAN-JACQUES, SERRAJ, RACHID
• Background and Aims The inner cortical cells (IC-cells) of legume root nodules have been previously shown to regulate the resistance to nodule O2 diffusion by a rapid contraction/expansion...
Mimura, Hisatoshi, Nakanishi, Yoichi, Hirono, Megumi, Maeshima, Masayoshi
The H^+-translocating pyrophosphatase (H^+-PPase) is a proton pump that is found in a wide variety of organisms. It consists of a single polypeptide chain that is thought to possess between 14 and 17...
Kamiya, Takehiro, Maeshima, Masayoshi
In plants, the cation/H^+ exchanger translocates Ca^{2+}and other metal ions into vacuoles using the H^+ gradient formed by H^+-ATPase and H^+-pyrophosphatase. Such exchangers carrying 11...
Suga, Shinobu, Maeshima, Masayoshi
Plants contain a number of aquaporin isoforms. We developed a method for determining the water channel activity of individual isoforms of aquaporin. Six plasma membrane aquaporins (RsPIPs) and two...
Yano, Kanako, Matsui, Sumiko, Tsuchiya, Tomohiro, Maeshima, Masayoshi, Kutsuna, Natsumaro, Hasezawa, Seiichiro, ...
Autolysosomes accumulate in tobacco cells cultured under sucrose starvation conditions in the presence of a cysteine protease inhibitor. We characterized these plant autolysosomes using fluorescent...
Kobae, Yoshihiro, Uemura, Tomohiro, Sato, Masa H., Ohnishi, Miwa, Mimura, Tetsuro, Nakagawa, Tsuyoshi, ...
Cation diffusion facilitator (CDF) proteins belong to a family of heavy metal efflux transporters that might play an essential role in homeostasis and tolerance to metal ions. We investigated the...
Anderca, Marinela Ioana, Suga, Shinobu, Furuichi, Takuya, Shimogawara, Kosuke, Maeshima, Masayoshi, Muto, Shoshi
By searching a Chlamydomonas expressed sequence tag database and by comparing the retrieved data with homologous sequences from a DNA database, we identified an expressed Chlamydomonas reinhardtii...
Fukuda, Atsunori, Chiba, Kazuhiro, Maeda, Miki, Nakamura, Atsuko, Maeshima, Masayoshi, Tanaka, Yoshiyuki
Two cDNA clones encoding vacuolar H+-inorganic pyrophosphatase (HVP1 and HVP10), one clone encoding the catalytic subunit (68 kDa) of vacuolar H+-ATPase (HvVHA-A), and one clone encoding vacuolar...
Fukuda, Atsunori, Chiba, Kazuhiro, Maeda, Miki, Nakamura, Atsuko, Maeshima, Masayoshi, Tanaka, Yoshiyuki
Two cDNA clones encoding vacuolar H+‐inorganic pyrophosphatase (HVP1 and HVP10), one clone encoding the catalytic subunit (68 kDa) of vacuolar H+‐ATPase (HvVHA‐A), and one...
Suga, Shinobu, Murai, Mari, Kuwagata, Tsuneo, Maeshima, Masayoshi
We investigated tissue- and cell-specific accumulation of radish aquaporin isoforms by immunocytochemical analysis. In taproots, the plasma membrane aquaporins (RsPIP1 and RsPIP2) were accumulated at...
Patch Clamp Analysis of a H+ Pump Heterologously Expressed in Giant Yeast Vacuoles (2003)
Nakanishi, Yoichi, Yabe, Isamu, Maeshima, Masayoshi
Despite the usefulness of the patch-clamp technique, its application to ion pumps and transporters in biomembranes is limited. We developed a novel method for determining the activity of a...
Hirono, Megumi, Ojika, Makoto, Mimura, Hisatoshi, Nakanishi, Yoichi, Maeshima, Masayoshi
H+-pyrophosphatase (H+-PPase), which pumps H+ across membranes coupled with PPi hydrolysis, is found in most plants, and some parasitic protists, eubacteria and archaebacteria. We...
Suga, Shinobu, Komatsu, Setsuko, Maeshima, Masayoshi
Aquaporins in the plasma and vacuolar membranes play a key role in the intercellular and intracellular water transport in plants. First, we quantitated the absolute amounts for mRNAs of eight...
Fujiwara, Shiori, Matsuda, Nao, Sato, Tomohiro, Sonobe, Seiji, Maeshima, Masayoshi
We cloned a cDNA for matrix-attachment region (MAR)-binding protein from Nicotiana tabacum cells to elucidate the structure and function of the nuclear matrix. The cDNA encodes a protein of 555 amino...
Ikeda, Mikiko, Umami, Kimiko, Hinohara, Misato, Tanimura, Yoshie, Ohmae, Atsuko, Nakanishi, Yoichi, ...
The function of the translation product of cDNA for Acetabularia vacuolar H+‐pyrophosphatase was examined using the Saccharomyces cerevisiae VMA3‐deficient strain. The open reading...
Ohshima, Yuko, Iwasaki, Ikuko, Suga, Shinobu, Murakami, Masanori, Inoue, Keiko, Maeshima, Masayoshi
Aquaporin facilitates the osmotic water transport across biomembranes and is involved in the transcellular and intracellular water flow in plants. We immunochemically quantified the aquaporin level...
Ueoka-Nakanishi, Hanayo, Maeshima, Masayoshi
Vacuolar Ca2+/H+ antiporter VCAX1p, which contributes to the Ca2+ accumulation into vacuoles, was quantified by immunochemistry. The antiporter content in vacuolar membranes was...
Maeshima, Masayoshi, Nakayasu, Tsuyoshi, Kawauchi, Keiko, Hirata, Hajime, Shimmen, Teruo
Cycloprodigiosin hydrochloride (cPrG-HCl) at 10 nM uncoupled both H+-ATPase and H+-translocating pyrophosphatase in vacuolar membrane vesicles of mung bean, but did not inhibit the substrate...
Suzuki, Yasuo, Maeshima, Masayoshi, Yamaki, Shohei
The cDNA of vacuolar proton-translocating inorganic pyrophosphatase was cloned from pear fruit. It contained an open reading frame of 2,301 nucleotides, coding for a polypeptide of 767 amino acids....
Kasai, Minobu, Nakamura, Tadakiyo, Kudo, Naomi, Sato, Hiroshi, Maeshima, Masayoshi, Sawada, Shinichi
Compared to rye plants grown under normal conditions of mineral nutrients, those grown under deficient conditions of mineral nutrients were shown to have a high potential activity of the root...
Higuchi, Tatsuji, Suga, Shinobu, Tsuchiya, Tomohiro, Hisada, Hiromoto, Morishima, Shigeru, Okada, Yasunobu, ...
A major membrane intrinsic protein (VM23) in vacuoles of radish (Raphanus) tap root was investigated. The cDNAs for two isoforms of VM23, γ- and δ-VM23, encode polypeptides of 253 and 248 amino...
Takasu, Ayako, Nakanishi, Yoichi, Yamauchi, Tokiko, Maeshima, Masayoshi
Vacuolar H+-translocating inorganic pyrophosphatase is a single-protein enzyme and uses a simple substance as an energy donor. Functional domains of the enzyme were investigated by using...
Odaira, Masato, Yoshida, Shizuo, Maeshima, Masayoshi
Physiological changes were examined in the amount of a 50-kDa glycoprotein (gp50) that was recovered in a nuclear fraction from hypocotyls of mung bean (Vigna radiata) seedlings. Immunoblot analysis...
Shiratake, Katsuhiro, Kanayama, Yoshinori, Maeshima, Masayoshi, Yamaki, Shohei
Vacuolar H+-ATPase (V-ATPase) was purified from pear fruit and antibodies were raised against the subunits of 55 and 33 kDa. Antibodies against mung bean H+-pyro-phosphatase (V-PPase) and radish...
Odaira, Masato, Arakawa, Keita, Yoshida, Shizuo, Maeshima, Masayoshi
Nuclei from mung bean (Vigna radiata) hypocotyls contained two glycoproteins of 50 and 49 kDa, respectively, that reacted with concanavalin A. The glycoproteins were released from the nuclear...
Characterization of Proteins of Nuclear Envelopes Prepared from Mung Bean Hypocotyls (1994)
Odaira, Masato, Maeshima, Masayoshi
The polypeptide composition of nuclear envelopes prepared from hypocotyls of mung bean (Vigna radiata) was investigated. The tissue was homogenized in the presence of Triton X-100 and nuclei were...
Maeshima, Masayoshi, Mimura, Tetsuro, Sato, Toshiyuki
Vacuolar membranes isolated from several species including fern and moss exhibited pyro-phosphate-dependent H+ transport activity. On immunoblot analysis, H+ -pyrophosphatase was detected in the...
The Nuclear Gene for Subunit Vc of Sweet Potato Cytochrome c Oxidase (1993)
Nakagawa, Tsuyoshi, Maeshima, Masayoshi, Nakamura, Kenzo, Asahi, Tadashi
We have cloned the gene for subunit Vc of sweet potato cytochrome c oxidase using its cDNA as a probe. There is a single intron, located one nucleotide upstream of the start of the open reading...
Kasai, Minobu, Yamamoto, Yoko, Maeshima, Masayoshi, Matsumoto, Hideaki
We investigated the effects of in vivo treatment (1 day) of barley roots with abscisic acid (ABA) and/or a cytokinin (6-benzyladenine; BA) on the ATP- and PPi-dependent H+ transport activities of...
Nakagawa, Tsuyoshi, Maeshima, Masayoshi, Muto, Hideyo, Asahi, Tadashi
Subunit IV of cytochrome c oxidase from sweet potato was bound with dicyclohexylcarbodiimide and synthesized in isolated mitochondria. Thus, this subunit corresponds to subunit HI of the analogous...
Tissue-Specific Isoforms of Catalase Subunits in Castor Bean Seedlings (1992)
Ota, Yoko, Ario, Takeshi, Hayashi, Koji, Nakagawa, Tsuyoshi, Hattori, Tsukaho, Maeshima, Masayoshi, ...
Catalases purified from endosperm glyoxysomes and non-specialized microbodies from hypocotyls of castor bean seedlings differed in their specific activity [90–164 and 0.89–4.9 kunits (mg...
Maeshima, Masayoshi, Nakagawa, Tsuyoshi, Asahi, Tadashi
The N-terminal amino acid sequence of sweet potato cytochrome c oxidase subunit II polypeptide was determined. Comparisons between the sequence and amino acid sequences deduced from the nucleotide...
Kimura, Tetsuya, Maeshima, Masayoshi, Asahi, Tadashi
ATPase in a highly purified plasma membrane fraction from mung bean hypocotyls was solubilized by lysolecithin and fractionated by glycerol density gradient centrifugation. Lysolecithin activated...
Maeshima, Masayoshi, Yokoi, Hiroyuki, Asahi, Tadashi
The amino-terminal sequence of isocitrate lyase purified from castor bean endosperm glyoxysomes was compared with that deduced from the nucleotide sequence of cDNA for the enzyme [Plant Mol. Biol....
Differences in the Thermostability and Subunit Species of Cytochrome c Oxidase among Tissues (1988)
Nakagawa, Tsuyoshi, Maeshima, Masayoshi, Asahi, Tadashi
Cytochrome c oxidase associated with the mitochondrial inner membrane of the overground or underground organs of mung bean was more stable at 40–55�C than that of the corresponding organs of pea....
Maeshima, Masayoshi, Li, He-sheng, Asahi, Tadashi
Submitochondrial particles from sweet potato root tissue retained the respiratory characteristics of the intact mitochondria with respect to the sensitivity to cyanide and salicylhydroxamic acid. The...
Esaka, Muneharu, Maeshima, Masayoshi, Asahi, Tadashi
An increase in catalase activity accompanied by microbody development in wounded sweet potato root tissue was investigated with a specific antibody against sweet potato catalase. The increase was...
高等植物の傷害組織におけるチトクロムオキシダーゼの生合成機構について (1981)
名古屋大学博士学位論文 学位の種類:農学博士 (課程) 学位授与年月日:昭和56年3月25日
高等植物の傷害組織におけるチトクロムオキシダーゼの生合成機構について (1981)
名古屋大学博士学位論文 学位の種類:農学博士 (課程) 学位授与年月日:昭和56年3月25日
The Subunit Composition of Pea Cytochrome c Oxidase (1981)
MATSUOKA, Makoto, MAESHIMA, Masayoshi, ASAHI, Tadashi
Cytochrome c oxidase was purified from pea shoots in a form containing more than 12 nmol of heme a per mg protein, but rapid inactivation took place during purification. On slab polyacrylamide...
MAESHIMA, Masayoshi, ASAHI, Tadashi
The mechanism of an increase in cytochrome c oxidase [EC 1.9.3.1] activity during aging of sliced sweet potato root tissue was investigated with antibiotics and antibody to the purified enzyme. 1....
MAESHIMA, Masayoshi, ASAHI, Tadashi
A protein, which was immunoreactive to antibody against cytochrome c oxidase, was found in the mitochondrial membrane fraction of sweet potato root tissue. The protein was associated relatively...
細胞ダイナミクス研究室(名古屋大学大学院生命農学研究科)のホームページへようこそ
前島, 正義, マエシマ, マサヨシ, Maeshima, Masayoshi
名古屋大学大学院生命農学研究科生物機構・機能科学専攻バイオダイナミクス講座細胞ダイナミクス研究室のホームページ。研究背景、構成員、業...
細胞ダイナミクス研究室(名古屋大学大学院生命農学研究科)のホームページへようこそ
名古屋大学大学院生命農学研究科生物機構・機能科学専攻バイオダイナミクス講座細胞ダイナミクス研究室のホームページ。研究背景、構成員、業...
Smart, Lawrence B., Vojdani, Fakrieh, Maeshima, Masayoshi, Wilkins, Thea A.
Cotton (Gossypium hirsutum L.) fibers are single-celled trichomes that synchronously undergo a phase of rapid cell expansion, then a phase including secondary cell wall deposition, and finally...
Nakanishi, Yoichi, Maeshima, Masayoshi
Vacuolar proton-translocating inorganic pyrophosphatase and H+-ATPase acidify the vacuoles and power the vacuolar secondary active transport systems in plants. Developmental changes in the...
Purification, Properties, and Molecular Cloning of a Novel Ca2+-Binding Protein in Radish Vacuoles1
Yuasa, Koji, Maeshima, Masayoshi
To understand the roles of plant vacuoles, we have purified and characterized a major soluble protein from vacuoles of radish (Raphanus sativus cv Tokinashi-daikon) taproots. The results showed that...
Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters
Geisler, Markus, Girin, Marjolaine, Brandt, Sabine, Vincenzetti, Vincent, Plaza, Sonia, Paris, Nadine, ...
Previously, the immunophilin-like protein TWD1 from Arabidopsis has been demonstrated to interact with the ABC transporters AtPGP1 and its closest homologue, AtPGP19. Physiological and biochemical...
Purification and Properties of Glyoxysomal Lipase from Castor Bean 1
Maeshima, Masayoshi, Beevers, Harry
The alkaline lipase in the glyoxysomes from the endosperm of young castor bean seedlings, an integral membrane component, was solubilized in deoxycholate:KCl and purified to apparent homogeneity. The...
Mechanism of the Decline in Vacuolar H+ -ATPase Activity in Mung Bean Hypocotyls during Chilling 1
Matsuura-Endo, Chie, Maeshima, Masayoshi, Yoshida, Shizuo
The mechanism responsible for the decrease in the activity of vacuolar H+ -ATPase during chilling was investigated in seedlings of mung bean (Vigna radiata). After chilling at 0°C for 3 d, the...
Characterization of the Major Integral Protein of Vacuolar Membrane 1
The vacuolar membrane of radish (Raphanus sativus) taproot contained a large quantity of a protein of 23 kilodaltons that accounted for more than 25% of the total membrane proteins. The protein,...
Smart, Lawrence B., Vojdani, Fakrieh, Maeshima, Masayoshi, Wilkins, Thea A.
Cotton (Gossypium hirsutum L.) fibers are single-celled trichomes that synchronously undergo a phase of rapid cell expansion, then a phase including secondary cell wall deposition, and finally...
Nakanishi, Yoichi, Maeshima, Masayoshi
Vacuolar proton-translocating inorganic pyrophosphatase and H+-ATPase acidify the vacuoles and power the vacuolar secondary active transport systems in plants. Developmental changes in the...
Purification, Properties, and Molecular Cloning of a Novel Ca2+-Binding Protein in Radish Vacuoles1
Yuasa, Koji, Maeshima, Masayoshi
To understand the roles of plant vacuoles, we have purified and characterized a major soluble protein from vacuoles of radish (Raphanus sativus cv Tokinashi-daikon) taproots. The results showed that...
Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters
Geisler, Markus, Girin, Marjolaine, Brandt, Sabine, Vincenzetti, Vincent, Plaza, Sonia, Paris, Nadine, ...
Previously, the immunophilin-like protein TWD1 from Arabidopsis has been demonstrated to interact with the ABC transporters AtPGP1 and its closest homologue, AtPGP19. Physiological and biochemical...
Purification and Properties of Glyoxysomal Lipase from Castor Bean 1
Maeshima, Masayoshi, Beevers, Harry
The alkaline lipase in the glyoxysomes from the endosperm of young castor bean seedlings, an integral membrane component, was solubilized in deoxycholate:KCl and purified to apparent homogeneity. The...
Mechanism of the Decline in Vacuolar H+ -ATPase Activity in Mung Bean Hypocotyls during Chilling 1
Matsuura-Endo, Chie, Maeshima, Masayoshi, Yoshida, Shizuo
The mechanism responsible for the decrease in the activity of vacuolar H+ -ATPase during chilling was investigated in seedlings of mung bean (Vigna radiata). After chilling at 0°C for 3 d, the...
Characterization of the Major Integral Protein of Vacuolar Membrane 1
The vacuolar membrane of radish (Raphanus sativus) taproot contained a large quantity of a protein of 23 kilodaltons that accounted for more than 25% of the total membrane proteins. The protein,...
細胞ダイナミクス研究室(名古屋大学大学院生命農学研究科)のホームページへようこそ
前島, 正義, マエシマ, マサヨシ, Maeshima, Masayoshi
名古屋大学大学院生命農学研究科生物機構・機能科学専攻バイオダイナミクス講座細胞ダイナミクス研究室のホームページ。研究背景、構成員、業...
The protein storage vacuole: a unique compound organelle
Jiang, Liwen, Phillips, Thomas E., Hamm, Christopher A., Drozdowicz, Yolanda M., Rea, Philip A., Maeshima, Masayoshi, ...
Storage proteins are deposited into protein storage vacuoles (PSVs) during plant seed development and maturation and stably accumulate to high levels; subsequently, during germination the storage...
Kawachi, Miki, Kobae, Yoshihiro, Mimura, Tetsuro, Maeshima, Masayoshi
Arabidopsis thaliana AtMTP1 belongs to the cation diffusion facilitator family and is localized on the vacuolar membrane. We investigated the enzymatic kinetics of AtMTP1 by a heterologous expression...