Ogawa, Katsuo, Tsuji, Takao, Namba, Masayoshi, Hayama, Kazuo, Okamoto, Tsukasa, Miwa, Yasuhiko
There is as yet no plausible and convincing explanation for the etiology of rheumatoid arthritis. The authors investigated anti-synovial tissue antibody in the body fluid of rheumatoid arthritis by...
In the experiments with cultured liver cells it is very important to know whether or not the cells in vitro have the same properties and functions as in vivo. The purposes of this work were to...
The liver cells obtained from a calf have been cultured continuously for 257 days in total at present (May 31, 1967). The primary culture was maintained in rotatory culture for about 2 months with...
Properties and identification of rat liver cells in long-term cultivation (2007)
Namba, Masayoshi, Hirose, Masaharu, Kishimoto, Ritsu
When cultured cells are used in experiments, It is very important to know from what kinds of cells the cultured cells are originated, and what characteristics the cultured cells maintain continuously...
Inhibitory effect of guinea pig serum on JTC-11 cell growth in vitro (2007)
Chikata, Eiji, Usui, Keiji, Nishizaki, Shizuo, Koshiba, Kimikazu, Tabuchi, Katsusuke, Namba, Masayoshi
The growth of JTC-11 cell line which was established from Ehrlich ascites carcinoma in vitro was inhibited by the addition of 2 per cent guinea pig serum to the control medium composed of 10 per cent...
Jahan, Israt, Bai, Liyan, Iijima, Mikio, Kondo, Tadashi, Namba, Masayoshi
The establishment of a model system of neoplastic transformation of normal human cells has been attempted with a chemical carcinogen, 4-nitroquinoline 1-oxide (4NQO). In the course of these...
Pu, Hong, Tsuji, Toshiya, Kondo, Asami, Fushimi, Kazuo, Ohashi, Ryuichiro, Inoue, Yusuke, ...
Characteristics of human hepatoma cell lines with the wild-type p53 were compared with those of human hepatoma cell lines with the mutant-type p53. The p21 protein located downstream of p53 was...
Mihara, Koichiro, Miyazaki, Masahiro, Kondo, Tadashi, Fushimi, Kazuo, Tsuji, Toshiya, Inoue, Yusuke, ...
We used a yeast functional assay (functional analysis of separated alleles in yeast: FASAY) to determine the p53 gene status of human cell lines maintained in our laboratory. This assay enables the...
Effects of simulated microgravity on human osteoblast-like cells in culture (2007)
Kunisada, Toshiyuki, Kawai, Akira, Inoue, Hajime, Namba, Masayoshi
Physiological strain plays an important role in maintaining the normal function and metabolism of bone cells. It is well known that the mineral content of astronauts' bones decreases during...
Enhancement of DNA-transfection frequency by X-rays (2007)
Iwamoto, Ryota, Fushimi, Kazuo, Hiraki, Yoshio, Namba, Masayoshi
This study was conducted to evaluate the frequency of DNA transfection into human cells following X-ray irradiation. We transfected plasmid DNA (pSV2neo) into human cells, HeLa and PA-1, by either...
Immortalization of Cells from Brains Derived from a Strain (MSM/MSfB6C3Fl) of Wild Mouse (2007)
Yuasa, Takae, Abe, Eriko, Oshimura, Mitsuo, Namba, Masayoshi
Escape from cellular aging is the rate-limiting step of multistep carcinogenesis. While normal human cells invariably undergo cellular aging and almost never spontaneously immortalize, cells derived...
Kosaka, Tsunenori, Fukaya, Ken-ichi, Tsuboi, So, Pu, Hong, Ohno, Tadao, Tsuji, Takao, ...
The sensitivity of five kinds of cytotoxicity assays using ethanol on human hepatoblastoma cells (HUH-6 line), which were cultured as monolayers or spheroids, was compared. Ethanol was chosen as a...
Lijima, Mikio, Kano, Yoshio, Nohno, Tsutomu, Namba, Masayoshi
Normal human fibroblasts have a finite proliferative capacity in vitro. Thus, immortalization of human cells is associated with cellular aging. We have established an immortalization-sensitive cell...
Kosaka, Tsunenori, Tsuboi, So, Fukaya, Ken-ichi, Pu, Hong, Ohno, Tadao, Tsuji, Takao, ...
Spheroid cultures of human hepatoblastoma cells (HuH-6 line) were established by rotating 3 x 10(6) cells/3 ml culture medium in 25-ml Erlenmeyer flasks on a gyratory shaker. The size of the...
Kosaka, Tsunenori, Tsuboi, So, Fukaya, Ken-ichi, Pu, Hong, Ohno, Tadao, Tsuji, Takao, ...
Spheroid cultures of human hepatoblastoma cells (HuH-6 line) were established by rotating 3 x 10(6) cells/3 ml culture medium in 25-ml Erlenmeyer flasks on a gyratory shaker. The size of the...
Methanol stimulates the colony-formation rate in a human hepatoma cell line (HLE). (2007)
Gu, Yin-chun, Okada, Shin-ichi, Ohomori, Masakazu, Kitajiri, Shin-ichiro, Namba, Masayoshi
Effects of methanol on colony-formation of human hepatoma cells were investigated. Among five human hepatoma cell lines (Hep G2, HLE, HuH-6, HuH-7, and PLC/PRF/5), only HLE cells showed enhanced...
Antiproliferative effects of suramin on human cancer cells in vitro and in vivo. (2007)
Bai, Liyan, Naomoto, Yoshio, Miyazaki, Masahiro, Orita, Kunzo, Namba, Masayoshi
The present experiment was undertaken to study what types of human cancers are responsive to the antiproliferative effects of suramin. The human malignant cells used were as follows: cervical cancer...
Miyazaki, Masahiro, Bai, Liyan, Tsuboi, So, Seshimo, Ken, Namba, Masayoshi
Effects of antioxidants, such as superoxide dismutase, vitamin C, vitamin E, 4-(0-benzylphenoxy)-N-methylbutylamine hydrochloride (bifemelane), and selenite on survival of adult rat hepatocytes were...
Effects of antioxidants on V79 Chinese hamster cells treated with ferric nitrilotriacetate. (2007)
Takehara, Yoshiki, Yoshioka, Tamotsu, Namba, Masayoshi
The cytotoxic effects of ferric nitrilotriacetate (Fe-NTA) have been considered to be caused by free radicals produced by the drug. The present study was carried out to determine whether or not...
Localization of S100C immunoreactivity in various human tissues. (2006)
Kondo, Asami, Sakaguchi, Masakiyo, Makino, Eiichi, Namba, Masayoshi, Okada, Shigeru, Huh, Nam-ho
Using 2-dimensional gel electrophoresis, we previously demonstrated that the S100C protein remarkably decreased after immortalization of normal human fibroblasts, and that this protein caused growth...
Protection of mice from LPS-induced shock by CD14 antisense oligonucleotide. (2006)
Furusato, Shoji, Takahashi, Tomohiro, Mori, Sadao, Takahashi, Yasuo, Tsuda, Toshihiko, Namba, Masayoshi, ...
CD14 is a pattern recognition receptor on myeloid cells and plays a pivotal role in an innate immune system that is responsible for Gram-negative and Gram-positive bacteria infection....
Protein Transduction Assisted by Polyethylenimine-Cationized Carrier Proteins (2005)
Kitazoe, Midori, Murata, Hitoshi, Futami, Junichiro, Maeda, Takashi, Sakaguchi, Masakiyo, Miyazaki, Masahiro, ...
Previously, we have reported that cationized-proteins covalently modified with polyethylenimine (PEI) (direct PEI-cationization) efficiently enter cells and function in the cytosol [Futami et...
Furumatsu, Takayuki, Yamaguchi, Noriko, Nishida, Keiichiro, Kawai, Akira, Kunisada, Toshiyuki, Namba, Masayoshi, ...
Endostatin derived from collagen XVIII is a potent endogenous anti-angiogenic factor that induces regression of various tumors of epithelial origin. Endostatin has been shown to inhibit endothelial...
KEN-ICHI FUKAYA, SATORU ASAHI, SEISHI NAGAMORI, MASAKIYO SAKAGUCHI, CHONG GAO, MASAHIRO MIYAZAKI, ...
Nakatsuka, Shigeharu, Tanaka, Hiroyoshi, Namba, Masayoshi
Ferric nitrilotriacetate (Fe-NTA) induced dose- and time-dependent mutation of V79 Chinese hamster cells to 6-thioguanine resistance. It also caused dose-related inhibition of metabolic cooperation....
B-Cell Differentiation Antigen Expressed on a Human Myeloma Cell Line (KMM-1) (1983)
TOGAWA, ATSUSHI, INOUE, NOBUMASA, MANNOJI, MASAKIYO, NAMBA, MASAYOSHI
KMM-1 is a human multiple myeloma cell line previously shown to express plasma cell phenotypes. Hybridomas against this cell line were produced by fusing spleen cells from hyperimmunized BALB/c...
Sakaguchi, Masakiyo, Sonegawa, Hiroyuki, Nukui, Takamasa, Sakaguchi, Yoshihiko, Miyazaki, Masahiro, Namba, Masayoshi, ...
Growth suppression of normal human keratinocytes by high Ca2+ or TGFβ was shown to be mediated by p21WAF1/CIP1 and Sp1 [Pardali, K., et al. (2000) J. Biol. Chem. 275, 29244–29256; Santini, M. P.,...
Sakaguchi, Masakiyo, Sonegawa, Hiroyuki, Nukui, Takamasa, Sakaguchi, Yoshihiko, Miyazaki, Masahiro, Namba, Masayoshi, ...
Growth suppression of normal human keratinocytes by high Ca2+ or TGFβ was shown to be mediated by p21WAF1/CIP1 and Sp1 [Pardali, K., et al. (2000) J. Biol. Chem. 275, 29244–29256; Santini, M. P.,...
PKCα mediates TGFβ-induced growth inhibition of human keratinocytes via phosphorylation of S100C/A11
Sakaguchi, Masakiyo, Miyazaki, Masahiro, Sonegawa, Hiroyuki, Kashiwagi, Mariko, Ohba, Motoi, Kuroki, Toshio, ...
Growth regulation of epithelial cells is of major concern because most human cancers arise from them. We demonstrated previously a novel signal pathway involving S100C/A11 for high Ca2+-induced...
S100C/A11 is a key mediator of Ca2+-induced growth inhibition of human epidermal keratinocytes
Sakaguchi, Masakiyo, Miyazaki, Masahiro, Takaishi, Mikiro, Sakaguchi, Yoshihiko, Makino, Eiichi, Kataoka, Noriyuki, ...
An increase in extracellular Ca2+ induces growth arrest and differentiation of human keratinocytes in culture. We examined possible involvement of S100C/A11 in this growth regulation. On exposure of...
Relationship between Contact Inhibition and Intranuclear S100c of Normal Human Fibroblasts
Sakaguchi, Masakiyo, Miyazaki, Masahiro, Inoue, Yusuke, Tsuji, Toshiya, Kouchi, Hirosuke, Tanaka, Toshio, ...
Many lines of evidence indicate that neoplastic transformation of cells occurs by a multistep process. For neoplastic transformation of normal human cells, they must be first immortalized and then be...