Control of electric current by graphene edge structure engineering (2009)
Yamamoto, Masayuki, Wakabayashi, Katsunori
In graphene nanoribbon junctions, the nearly perfect transmission occurs in some junctions while the zero conductance dips due to anti-resonance appear in others. We have classified the appearance of...
Electronic transport properties of graphene nanoribbons (2009)
Wakabayashi, Katsunori, Takane, Yositake, Yamamoto, Masayuki, Sigrist, Manfred
We will present brief overview on the electronic and transport properties of graphene nanoribbons focusing on the effect of edge shapes and impurity scattering. The low-energy electronic states of...
Kohn Anomalies in Graphene Nanoribbons (2009)
Sasaki, Ken-ichi, Yamamoto, Masayuki, Murakami, Shuichi, Saito, Riichiro, Dresselhaus, Mildred S., Takai, Kazuyuki, ...
The quantum corrections to the energies of the $\Gamma$ point optical phonon modes (Kohn anomalies) in graphene nanoribbons are investigated. We show theoretically that the longitudinal optical modes...
Conductance Distribution in Disordered Quantum Wires with a Perfectly Conducting Channel (2009)
Takane, Yositake, Iwasaki, Shingo, Yoshioka, Yuka, Yamamoto, Masayuki, Wakabayashi, Katsunori
We study the conductance of phase-coherent disordered quantum wires focusing on the case in which the number of conducting channels is imbalanced between two propagating directions. If the number of...
Nearly Perfect Single-Channel Conduction in Disordered Armchair Nanoribbons (2009)
Yamamoto, Masayuki, Takane, Yositake, Wakabayashi, Katsunori
The low-energy spectrum of graphene nanoribbons with armchair edges (armchair nanoribbons) is described as the superposition of two non-equivalent Dirac points of graphene. In spite of the lack of...
Senda, Eri, Fujimoto, Koji, Ohnishi, Katsuhiro, Higashida, Akihiro, Ashida, Cho, Okutani, Toshio, ...
Abstract Background Carcinoid tumors are usually considered to have a low degree of malignancy and show slow progression. One of the factors indicating the malignancy of these tumors is their size,...
Nohara, Keiko, Suzuki, Takehiro, Ao, Kana, Murai, Hikari, Miyamoto, Yoshimi, Inouye, Kaoru, ...
The ligand-dependent transcription factor aryl hydrocarbon receptor (AhR) has been implicated in various immune functions. Our previous studies have shown that AhR activation by exposure of ovalbumin...
Yates, Melinda S., Tran, Quynh T., Dolan, Patrick M., Osburn, William O., Shin, Soona, McCulloch, Colin C., ...
Loss of NF-E2-related factor 2 (Nrf2) signaling increases susceptibility to acute toxicity, inflammation and carcinogenesis in mice due to the inability to mount adaptive responses. In contrast,...
Reisman, Scott A., Yeager, Ronnie L., Yamamoto, Masayuki, Klaassen, Curtis D.
Nuclear factor erythroid 2–related factor 2 (Nrf2) is a transcription factor critical for protection against electrophilic and oxidative stress. In a recently engineered mouse with knockdown of...
Kentaro Yomogida, Haruo Ohtani, Hideo Harigae, Etsuro Ito, Yoshitake Nishimune, James Douglas, ...
*Author for correspondence
Wakabayashi, Katsunori, Takane, Yositake, Yamamoto, Masayuki, Sigrist, Manfred
Numerical calculations have been performed to elucidate unconventional electronic transport properties in disordered nanographene ribbons with zigzag edges (zigzag ribbons). The energy band structure...
Osburn, William O., Yates, Melinda S., Dolan, Patrick D., Chen, Sining, Liby, Karen T., Sporn, Michael B., ...
Oxidative stress–mediated destruction of normal parenchymal cells during hepatic inflammatory responses contributes to the pathogenesis of immune-mediated hepatitis and is implicated in the...
Nrf2 and p53 cooperatively protect against BBN-induced urinary bladder carcinogenesis (2007)
Iida, Katsuyuki, Itoh, Ken, Maher, Jonathan M., Kumagai, Yoshito, Oyasu, Ryoichi, Mori, Yukio, ...
Nuclear factor-erythroid 2 (NF-E2)-related factor 2 (Nrf2), a transcription factor that regulates inducible expression of detoxifying enzymes, is critical in preventing...
The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription (2007)
Tanabe, Osamu, Shen, Yannan, Liu, Qinghui, Campbell, Andrew D., Kuroha, Takashi, Yamamoto, Masayuki, ...
When the orphan nuclear receptors TR2 and TR4, the DNA-binding subunits of the DRED repressor complex, are forcibly expressed in erythroid cells of transgenic mice, embryos exhibit a transient...
Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate (2007)
Sawa, Tomohiro, Mohammad Hasan Zaki, Okamoto, Tatsuya, Akuta, Teruo, Tokutomi, Yoshiko, Kim-Mitsuyama, Shokei, ...
The signaling pathway of nitric oxide (NO) depends mainly on guanosine 3′,5′-cyclic monophosphate (cGMP, 1). Here we report the formation and chemical biology of a nitrated derivative of cGMP,...
Regulation of GATA1 Gene Expression (2007)
Kobayashi, Makoto, Yamamoto, Masayuki
GATA1 is one of the most fascinating transcription factors for biologists. It regulates many haematopoietic genes and interacts with a number of other transcription factors. Its functions are mostly...
Pan, Xiaoqing, Minegishi, Naoko, Harigae, Hideo, Yamagiwa, Hironori, Minegishi, Masayoshi, Akine, Yasuyuki, ...
Die Spin-Orbit Kopplung ist eine relativistische Korrektur zur Schroedingergleichung, die in Anwesenheit elektrischer Felder auftritt. Der Effekt ist ziemlich schwach und wird erst signifikant, wenn...
The evaluation of the use of imagery and goal orientation : a tale of two cultures / (2005)
Thesis (M.S.)--Western Illinois University, 2005.
Nishiyama, Chiharu, Ito, Tomonobu, Nishiyama, Makoto, Masaki, Shigehiro, Maeda, Keiko, Nakano, Nobuhiro, ...
The high-affinity receptor for IgE (FcεRI) that is expressed on the surface of mast cells plays an important role in antigen/IgE-mediated allergic reactions. We have previously found that...
Umemura, Takashi, Kuroiwa, Yuichi, Kitamura, Yasuki, Ishii, Yuji, Kanki, Keita, Kodama, Yukio, ...
Our goal was to elucidate roles of Nrf2 in in vivo defense against pentachlorophenol (PCP), an environmental pollutant and hepatocarcinogen in mice. We examined oxidative stress and cell...
Nishiyama, Chiharu, Ito, Tomonobu, Nishiyama, Makoto, Masaki, Shigehiro, Maeda, Keiko, Nakano, Nobuhiro, ...
The high-affinity receptor for IgE (FcϵRI) that is expressed on the surface of mast cells plays an important role in antigen/IgE-mediated allergic reactions. We have previously found that...
Mesoscopic Stern-Gerlach spin filter by nonuniform spin-orbit interaction (2004)
Ohe, Jun-ichiro, Yamamoto, Masayuki, Ohtsuki, Tomi, Nitta, Junsaku
A novel spin filtering in two-dimensional electron system with nonuniform spin-orbit interactions (SOI) is theoretically studied. The strength of SOI is modulated perpendicular to the charge current....
Ramos-Gomez, Minerva, Dolan, Patrick M., Itoh, Ken, Yamamoto, Masayuki, Kensler, Thomas W.
The cancer chemopreventive actions of oltipraz (4-methyl-5-[2-pyrazinyl]-1,2-dithiole-3-thione) have been primarily associated with the induction of phase 2 detoxifying enzymes through...
Proplatelet formation of megakaryocytes is triggered by autocrine-synthesized estradiol (2003)
Nagata, Yuka, Yoshikawa, Jun, Hashimoto, Atsushi, Yamamoto, Masayuki, Payne, Anita H., Todokoro, Kazuo
A matured megakaryocyte releases thousands of platelets through a drastic morphological change, proplatelet formation (PPF). The megakaryocyte/erythrocyte-specific transcription factor, p45 NF-E2, is...
Ishizaki, Kimitsune, Shimizu-Ueda, Yuu, Okada, Sachiko, Yamamoto, Masayuki, Fujisawa, Masaki, Yamato, Katsuyuki T., ...
Sex of the liverwort Marchantia polymorpha is determined by the sex chromosomes Y and X, in male and female plant, respectively. Approximately half of the Y chromosome is made up of unique repeat...
Sun, Jiying, Muto, Akihiko, Hoshino, Hideto, Kobayashi, Akira, Nishimura, Shigeko, Yamamoto, Masayuki, ...
The Maf recognition element (MARE) is regulated by both activators and repressors. Bachl and Bach2 repress MARE-dependent transcription by forming heterodimers with Maf-related oncoproteins. In order...
Enomoto, Akiko, Itoh, Ken, Nagayoshi, Eiko, Haruta, Junko, Kimura, Toyoe, O'Connor, Tania, ...
Nrf2, which belongs to the basic leucine zipper (bZip) transcription factor family, has been implicated as a key molecule involved in antioxidant-responsive element (ARE)-mediated gene expression. In...
Ishii, Tetsuro, Itoh, Ken, Akasaka, Junetsu, Yanagawa, Toru, Takahashi, Satoru, Yoshida, Hiroshi, ...
Feeding mice with 2(3)-t-butyl-4-hydroxyanisole (BHA) induces phase II detoxifying enzymes that inhibit the action of carcinogens. We have found that dietary BHA induces intestinal and hepatic MSP23...
Pan, Xiaoqing, Minegishi, Naoko, Harigae, Hideo, Yamagiwa, Hironori, Minegishi, Masayoshi, Akine, Yasuyuki, ...
Transcription factor GATA-2 is essential for the proper function of hematopoietic stem cells and progenitors. Two first exons/promoters have been found in the mouse GATA-2 gene, and a distal IS...
Wakabayashi, Nobunao, Kageyama, Ryoichiro, Habu, Toshiyuki, Doi, Toshihide, Morita, Takashi, Nozaki, Masami, ...
The regulatory mechanisms of mammalian hairy and Enhancer of split homologue-1 (HES-1) genes were examined in mouse P19 embryonic carcinoma cells (P19 cells). Undifferentiated P19 stem cells...
Itoh, Ken, Wakabayashi, Nobunao, Katoh, Yasutake, Ishii, Tetsuro, Igarashi, Kazuhiko, Engel, James Douglas, ...
Kuroha, Takashi, Takahashi, Satoru, Komeno, Takuya, Itoh, Ken, Nagasawa, Toshiro, Yamamoto, Masayuki
Maf recognition elements (MAREs or NF-E2 binding sites) have been shown to be vital for erythroid- and megakaryocyte-specific gene expression. Transcription factor NF-E2 is composed of p45, a large...
Impaired megakaryopoiesis and behavioral defects in mafG-null mutant mice (1998)
Shavit, Jordan A., Motohashi, Hozumi, Onodera, Ko, Akasaka, Jun-etsu, Yamamoto, Masayuki, Engel, James Douglas
Onodera, Ko, Yomogida, Kentaro, Suwabe, Naruyoshi, Takahashi, Satoru, Muraosa, Yasushi, Hayashi, Norio, ...
Transcription factor GATA-1 was first identified in erythroid cells, but was later shown to also be expressed in Sertoli cells of the mouse testis. GATA-1 transcription in testis initiates from a...
Nagai, Tadashi, Harigae, Hideo, Furuyama, Kazumichi, Munakata, Hiroshi, Hayashi, Norio, Endo, Kazuyasu, ...
We examined the effect of hemin, TGF-β1 and cytosine arabinoside (Ara-C) on the levels of mRNAs for the erythroid-specific 5-aminolevulinate synthase (ALAS-E) and γ-globin in various human...
Structure and Regulation of the Chicken GATA-3 Gene (1995)
Ishihara, Hajime, Engel, James Douglas, Yamamoto, Masayuki
To elucidate the mechanisms underlying the tissue-restricted expression of GATA factor transcription, we have isolated and analyzed the genomic chicken GATA-3 (cGATA-3) locus. Structural analysis of...
Structure and regulation of the chicken erythroid {delta}-aminolevulinate synthase gene (1994)
Lim, Kim-Chew, Ishihara, Hajime, Riddle, Robert D., Yang, Zhuoying, Andrews, Nancy, Yamamoto, Masayuki, ...
Erythroid cells regulate heme biosynthesis in a manner that is distinct from all other cell types. While heme negatively regulates the synthesis of the housekeeping δ-amlnolevullnate synthase...
Kuromori, Takashi, Yamamoto, Masayuki
We characterized three Arabidopsis thaliana cDNA clones that could rescue the sterile phenotype of the Schlzosaccharomyces pombe pde1 mutant, which Is defective In cAMP phosphodlesterase. The first...
Structure and Expression of the Gene Encoding Rat Nonspecific Form -Aminolevulinate Synthase (1993)
Yomogida, Kentaro, Yamamoto, Masayuki, Yamagami, Takashi, Fujita, Hiroyoshi, Hayashi, Norio
To understand the regulatory mechanisms controlling the heme biosynthetic pathway in animal liver, RNA blot hybridization analysis was used to examine the developmental stage-specific transcription...
Purification and Structure of Rat Erythroid-Specific {delta}-Aminolevulinate Synthase (1993)
Munakata, Hiroshi, Yamagami, Takashi, Nagai, Tadashi, Yamamoto, Masayuki, Hayashi, Norio
The existence of erythroid form δ-aminolevulinate synthase (ALAS-E) was historically a matter of some controversy. To obtain direct evidence for a unique ALAS-E, we have purified ALAS-E to...
Nakahashi, Yoshitsugu, Miyazaki, Hiroaki, Kadota, Yoichi, Naitoh, Yuji, Inoue, Kyoichi, Yamamoto, Masayuki, ...
Kunitomo, Hirofumi, Higuchi, Toru, Iino, Yuichi, Yamamoto, Masayuki
Schizosaccharomyces pombe ste11 encodes a high-mobility group family transcriptional activator that is pivotal in sexual development. Transcription of ste11 is induced by starvation of nutrients via...
Onodera, Ko, Takahashi, Satoru, Nishimura, Shigeko, Ohta, Jun, Motohashi, Hozumi, Yomogida, Kentaro, ...
Transcription factor GATA-1 is required for the terminal differentiation of both the primitive and definitive erythroid cell lineages, and yet the regulatory mechanisms of GATA-1 itself are not well...
Toya, Mika, Iino, Yuichi, Yamamoto, Masayuki
The fission yeast pob1 gene encodes a protein of 871 amino acids carrying an SH3 domain, a SAM domain, and a PH domain. Gene disruption and construction of a temperature-sensitive pob1 mutant...
Ramos-Gomez, Minerva, Kwak, Mi-Kyoung, Dolan, Patrick M., Itoh, Ken, Yamamoto, Masayuki, Talalay, Paul, ...
Induction of phase 2 enzymes, which neutralize reactive electrophiles and act as indirect antioxidants, appears to be an effective means for achieving protection against a variety of carcinogens in...
Nishimura, Shigeko, Takahashi, Satoru, Kuroha, Takashi, Suwabe, Naruyoshi, Nagasawa, Toshiro, Trainor, Cecelia, ...
A region located at kbp −3.9 to −2.6 5′ to the first hematopoietic exon of the GATA-1 gene is necessary to recapitulate gene expression in both the primitive and definitive erythroid lineages....
Novel WD-Repeat Protein Mip1p Facilitates Function of the Meiotic Regulator Mei2p in Fission Yeast
Shinozaki-Yabana, Satoko, Watanabe, Yoshinori, Yamamoto, Masayuki
In fission yeast, the onset of meiosis is triggered by activation of the RNA-binding protein Mei2p. We screened for a high-copy-number suppressor of the ectopic meiosis induced by expression of an...
Kobayashi, Akira, Yamagiwa, Hironori, Hoshino, Hideto, Muto, Akihiko, Sato, Kazushige, Morita, Masanobu, ...
Bach2 is a B-cell- and neuron-specific transcription repressor that forms heterodimers with the Maf-related oncoproteins. We show here that Bach2 activates transcription by interacting with its novel...
Novel Factor Highly Conserved among Eukaryotes Controls Sexual Development in Fission Yeast
Okazaki, Noriko, Okazaki, Koei, Watanabe, Yoshinori, Kato-Hayashi, Mariko, Yamamoto, Masayuki, Okayama, Hiroto
In the fission yeast Schizosaccharomyces pombe, the onset of sexual development is controlled mainly by two external signals, nutrient starvation and mating pheromone availability. We have isolated a...
Ogawa, Kazuhiro, Sun, Jiying, Taketani, Shigeru, Nakajima, Osamu, Nishitani, Chiaki, Sassa, Shigeru, ...
Heme controls expression of genes involved in the synthesis of globins and heme. The mammalian transcription factor Bach1 functions as a repressor of the Maf recognition element (MARE) by forming...
In vivo requirements for GATA-1 functional domains during primitive and definitive erythropoiesis
Shimizu, Ritsuko, Takahashi, Satoru, Ohneda, Kinuko, Engel, James Douglas, Yamamoto, Masayuki
GATA-1 is a transcription factor essential for erythroid/megakaryocytic cell differentiation. To investigate the contribution of individual domains of GATA-1 to its activity, transgenic mice...
An embryonic/fetal β-type globin gene repressor contains a nuclear receptor TR2/TR4 heterodimer
Tanabe, Osamu, Katsuoka, Fumiki, Campbell, Andrew D., Song, Weimin, Yamamoto, Masayuki, Tanimoto, Keiji, ...
We recently described an erythroid ε-globin gene repressor activity, which we named DRED (direct repeat erythroid-definitive). We show that DRED binds with high affinity to DR1 sites in the human...
Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
Sun, Jiying, Hoshino, Hideto, Takaku, Kazuaki, Nakajima, Osamu, Muto, Akihiko, Suzuki, Hiroshi, ...
Heme oxygenase-1 (HO-1) protects cells from various insults including oxidative stress. Transcriptional activators, including the Nrf2/Maf heterodimer, have been the focus of studies on the inducible...
Dinkova-Kostova, Albena T., Holtzclaw, W. David, Cole, Robert N., Itoh, Ken, Wakabayashi, Nobunao, Katoh, Yasutake, ...
Coordinate induction of phase 2 proteins and elevation of glutathione protect cells against the toxic and carcinogenic effects of electrophiles and oxidants. All inducers react covalently with thiols...
Kwak, Mi-Kyoung, Itoh, Ken, Yamamoto, Masayuki, Kensler, Thomas W.
Induction of phase 2 enzymes, which neutralize reactive electrophiles and act as indirect antioxidants, is an important mechanism for protection against carcinogenesis. The transcription factor Nrf2,...
Higuchi, Toru, Watanabe, Yoshinori, Yamamoto, Masayuki
Protein kinase A (PKAi a cyclic AMP-dependent protein kinase) negatively regulates sexual development and gluconeogenesis in fission yeast by suppressing the transcription of ste11 required for the...
Ishizaki, Kimitsune, Shimizu-Ueda, Yuu, Okada, Sachiko, Yamamoto, Masayuki, Fujisawa, Masaki, Yamato, Katsuyuki T., ...
Sex of the liverwort Marchantia polymorpha is determined by the sex chromosomes Y and X, in male and female plant, respectively. Approximately half of the Y chromosome is made up of unique repeat...
Katsuoka, Fumiki, Motohashi, Hozumi, Tamagawa, Yuna, Kure, Shigeo, Igarashi, Kazuhiko, Engel, James Douglas, ...
The small Maf proteins form heterodimers with CNC and Bach family proteins to elicit transcriptional responses from Maf recognition elements (MAREs). We previously reported germ line-targeted...
HLF/HIF-2α is a key factor in retinopathy of prematurity in association with erythropoietin
Morita, Masanobu, Ohneda, Osamu, Yamashita, Toshiharu, Takahashi, Satoru, Suzuki, Norio, Nakajima, Osamu, ...
An HLF (HIF-1α-like factor)/HIF-2α-knockout mouse is embryonic lethal, preventing investigation of HLF function in adult mice. To investigate the role of HLF in adult pathological angiogenesis, we...
Functional Analysis of Basic Transcription Element Binding Protein by Gene Targeting Technology
Morita, Masanobu, Kobayashi, Akira, Yamashita, Toshiharu, Shimanuki, Tomomasa, Nakajima, Osamu, Takahashi, Satoru, ...
Basic transcription element binding protein (BTEB) is a transcription factor with a characteristic zinc finger motif and is most remarkably enhanced by thyroid hormone T3 treatment (R. J. Denver et...
Distinct response to dioxin in an arylhydrocarbon receptor (AHR)-humanized mouse
Moriguchi, Takashi, Motohashi, Hozumi, Hosoya, Tomonori, Nakajima, Osamu, Takahashi, Satoru, Ohsako, Seiichiroh, ...
There are large inter- and intraspecies differences in susceptibility to dioxin-induced toxicities. A critical question in risk assessment of dioxin and related compounds is whether humans are...
Matsuo, Tomohiko, Kubo, Yoshiya, Watanabe, Yoshinori, Yamamoto, Masayuki
The TOR protein is a phosphoinositide kinase-related kinase widely conserved among eukaryotes. Fission yeast tor1 encodes an ortholog of TOR, which is required for sexual development and growth under...
Mito, Yoshiko, Sugimoto, Asako, Yamamoto, Masayuki
Cohesin, which mediates sister chromatid cohesion, is composed of four subunits, named Scc1/Rad21, Scc3, Smc1, and Smc3 in yeast. Caenorhabditis elegans has a single homolog for each of Scc3, Smc1,...
Shimada, Tadayuki, Yamashita, Akira, Yamamoto, Masayuki
Fission yeast Mei2p is an RNA-binding protein essential for induction of both premeiotic DNA synthesis and first meiotic division. Mei2p forms a dot structure at an apparently fixed position in the...
Hirota, Kouji, Tanaka, Kayoko, Ohta, Kunihiro, Yamamoto, Masayuki
Fission yeast Cdc42p, a small GTPase of the Rho family, is essential for cell proliferation and maintenance of the rod-like cell morphology. Scd1/Ral1p is a GDP-GTP exchange factor (GEF) for Cdc42p....
One enhancer mediates mafK transcriptional activation in both hematopoietic and cardiac muscle cells
Katsuoka, Fumiki, Motohashi, Hozumi, Onodera, Ko, Suwabe, Naruyoshi, Engel, James Douglas, Yamamoto, Masayuki
Members of the small Maf family of transcription factors play important roles in hematopoiesis. Using transgenic assays, we discovered a tissue-specific enhancer 3′ to the mafK gene. This enhancer...
Rec8 cleavage by separase is required for meiotic nuclear divisions in fission yeast
Kitajima, Tomoya S., Miyazaki, Yousuke, Yamamoto, Masayuki, Watanabe, Yoshinori
Sister chromatid cohesion in meiosis is established by cohesin complexes, including the Rec8 subunit. During meiosis I, sister chromatid cohesion is destroyed along the chromosome arms to release...
Self-Association of Gata1 Enhances Transcriptional Activity In Vivo in Zebra Fish Embryos
Nishikawa, Keizo, Kobayashi, Makoto, Masumi, Atsuko, Lyons, Susan E., Weinstein, Brant M., Liu, P. Paul, ...
Gata1 is a prototype transcription factor that regulates hematopoiesis, yet the molecular mechanisms by which Gata1 transactivates its target genes in vivo remain unclear. We previously showed, in...
Antioxidants Enhance Mammalian Proteasome Expression through the Keap1-Nrf2 Signaling Pathway
Kwak, Mi-Kyoung, Wakabayashi, Nobunao, Greenlaw, Jennifer L., Yamamoto, Masayuki, Kensler, Thomas W.
Proteasomes degrade damaged proteins formed during oxidative stress, thereby promoting cell survival. Neurodegenerative and other age-related disorders are associated with reduced proteasome...
Proplatelet formation of megakaryocytes is triggered by autocrine-synthesized estradiol
Nagata, Yuka, Yoshikawa, Jun, Hashimoto, Atsushi, Yamamoto, Masayuki, Payne, Anita H., Todokoro, Kazuo
A matured megakaryocyte releases thousands of platelets through a drastic morphological change, proplatelet formation (PPF). The megakaryocyte/erythrocyte-specific transcription factor, p45 NF-E2, is...
Itoh, Ken, Mochizuki, Mie, Ishii, Yukio, Ishii, Tetsuro, Shibata, Takahiro, Kawamoto, Yoshiyuki, ...
Activated macrophages express high levels of Nrf2, a transcription factor that positively regulates the gene expression of antioxidant and detoxication enzymes. In this study, we examined how Nrf2...
Perinatal synthetic lethality and hematopoietic defects in compound mafG::mafK mutant mice
Onodera, Ko, Shavit, Jordan A., Motohashi, Hozumi, Yamamoto, Masayuki, Engel, James Douglas
Prior studies exploring the mechanisms controlling erythroid gene regulation implicated MARE (Maf recognition element) cis–elements as crucial to the transcriptional activity of many erythroid...
Itoh, Ken, Wakabayashi, Nobunao, Katoh, Yasutake, Ishii, Tetsuro, Igarashi, Kazuhiko, Engel, James Douglas, ...
Transcription factor Nrf2 is essential for the antioxidant responsive element (ARE)-mediated induction of phase II detoxifying and oxidative stress enzyme genes. Detailed analysis of differential...
Impaired megakaryopoiesis and behavioral defects in mafG-null mutant mice
Shavit, Jordan A., Motohashi, Hozumi, Onodera, Ko, Akasaka, Jun-etsu, Yamamoto, Masayuki, Engel, James Douglas
The small Maf proteins (MafG, MafK, and MafF), which serve as heterodimeric partner molecules of CNC family proteins for binding in vitro to MARE sites, have been implicated in the regulation of both...
Wakabayashi, Nobunao, Dinkova-Kostova, Albena T., Holtzclaw, W. David, Kang, Moon-Il, Kobayashi, Akira, Yamamoto, Masayuki, ...
Induction of a family of phase 2 genes encoding for proteins that protect against the damage of electrophiles and reactive oxygen intermediates is potentially a major strategy for reducing the risk...
Kang, Moon-Il, Kobayashi, Akira, Wakabayashi, Nobunao, Kim, Sang-Geon, Yamamoto, Masayuki
Transcription factor Nrf2 regulates basal and inducible expression of phase 2 proteins that protect animal cells against the toxic effects of electrophiles and oxidants. Under basal conditions, Nrf2...
Negative control for the initiation of meiosis in Schizosaccharomyces pombe
Iino, Yuichi, Yamamoto, Masayuki
Temperature-sensitive mutants of the pat1 gene of Schizosaccharomyces pombe are able to produce poorly viable spores from the haploid state without the requirement for nitrogen starvation if exposed...
Motohashi, Hozumi, Katsuoka, Fumiki, Engel, James Douglas, Yamamoto, Masayuki
The small Maf proteins, MafF, MafG, and MafK, possess a leucine zipper (Zip) domain that is required for homodimer or heterodimer complex formation with other bZip transcription factors. In this...
Kobayashi, Akira, Kang, Moon-Il, Okawa, Hiromi, Ohtsuji, Makiko, Zenke, Yukari, Chiba, Tomoki, ...
Transcription factor Nrf2 is a major regulator of genes encoding phase 2 detoxifying enzymes and antioxidant stress proteins in response to electrophilic agents and oxidative stress. In the absence...
Inoue, Takao, Sugimoto, Asako, Suzuki, Yuka, Yamamoto, Masayuki, Tsujimoto, Masafumi, Inoue, Keizo, ...
Type II platelet-activating factor-acetylhydrolase [PAF-AH (II)] is an N-myristoylated enzyme that contains a lipase/esterase catalytic motif and selectively hydrolyzes the sn-2 acetyl ester of PAF...
Genetic ablation of Nrf2 enhances susceptibility to cigarette smoke–induced emphysema in mice
Rangasamy, Tirumalai, Cho, Chung Y., Thimmulappa, Rajesh K., Zhen, Lijie, Srisuma, Sorachai S., Kensler, Thomas W., ...
Although inflammation and protease/antiprotease imbalance have been postulated to be critical in cigarette smoke–induced (CS-induced) emphysema, oxidative stress has been suspected to play an...
Khandekar, Melin, Suzuki, Norio, Lewton, Jon, Yamamoto, Masayuki, Engel, James Douglas
Transcription factor GATA-2 is expressed in a complex temporally and tissue-specific pattern within the developing embryo. Loss-of-function studies in the mouse showed that GATA-2 activity is first...
Leukemogenesis Caused by Incapacitated GATA-1 Function
Shimizu, Ritsuko, Kuroha, Takashi, Ohneda, Osamu, Pan, Xiaoqing, Ohneda, Kinuko, Takahashi, Satoru, ...
GATA-1 is essential for the development of erythroid and megakaryocytic lineages. We found that GATA-1 gene knockdown female (GATA-1.05/X) mice frequently develop a hematopoietic disorder resembling...
Molecular Determinants of NOTCH4 Transcription in Vascular Endothelium
Wu, Jing, Iwata, Fumiko, Grass, Jeffrey A., Osborne, Cameron S., Elnitski, Laura, Fraser, Peter, ...
The process whereby the primitive vascular network develops into the mature vasculature, known as angiogenic vascular remodeling, is controlled by the Notch signaling pathway. Of the two mammalian...
GATA1 Function, a Paradigm for Transcription Factors in Hematopoiesis
Ferreira, Rita, Ohneda, Kinuko, Yamamoto, Masayuki, Philipsen, Sjaak
Kelly, Vincent P., Suzuki, Takafumi, Nakajima, Osamu, Arai, Tsuyoshi, Tamai, Yoshitaka, Takahashi, Satoru, ...
Appropriate expression of the selenocysteine tRNA (tRNASec) gene is necessary for the production of an entire family of selenoprotein enzymes. This study investigates the consequence of disrupting an...
MafA Is a Key Regulator of Glucose-Stimulated Insulin Secretion
Zhang, Chuan, Moriguchi, Takashi, Kajihara, Miwako, Esaki, Ritsuko, Harada, Ayako, Shimohata, Homare, ...
MafA is a transcription factor that binds to the promoter in the insulin gene and has been postulated to regulate insulin transcription in response to serum glucose levels, but there is no current in...
Mating pheromone-like diffusible factor released by Schizosaccharomyces pombe
Fukui, Yasuhisa, Kaziro, Yoshito, Yamamoto, Masayuki
We demonstrate that a diffusible factor is secreted by h− cells of the fission yeast Schizosaccharomyces pombe, whose mating pheromones have not been described. This factor, tentatively named the...
Suzuki, Takafumi, Takagi, Yaeko, Osanai, Hitoshi, Li, Li, Takeuchi, Miki, Katoh, Yasutake, ...
Pi class GSTs (glutathione S-transferases) are a member of the vertebrate GST family of proteins that catalyse the conjugation of GSH to electrophilic compounds. The expression of Pi class GST genes...
GATA Motifs Regulate Early Hematopoietic Lineage-Specific Expression of the Gata2 Gene
Kobayashi-Osaki, Maki, Ohneda, Osamu, Suzuki, Norio, Minegishi, Naoko, Yokomizo, Tomomasa, Takahashi, Satoru, ...
Transcription factor GATA-2 is essential for definitive hematopoiesis, which developmentally emerges from the para-aortic splanchnopleura (P-Sp). The expression of a green fluorescent protein (GFP)...
Chanas, Simon A, Jiang, Qing, McMahon, Michael, McWalter, Gail K, McLellan, Lesley I, Elcombe, Clifford R, ...
Mice that lack the Nrf2 basic-region leucine-zipper transcription factor are more sensitive than wild-type (WT) animals to the cytotoxic and genotoxic effects of foreign chemicals and oxidants. To...
Nioi, Paul, McMahon, Michael, Itoh, Ken, Yamamoto, Masayuki, Hayes, John D
NQO1 [NAD(P)H:quinone oxidoreductase 1] has an integral role in cellular responses to oxidative stress. The expression of NQO1 is up-regulated in the mouse following challenge with electrophilic...
Katsuoka, Fumiki, Motohashi, Hozumi, Ishii, Tetsuro, Aburatani, Hiroyuki, Engel, James Douglas, Yamamoto, Masayuki
While small Maf proteins have been suggested to be essential for the Nrf2-mediated activation of antioxidant response element (ARE)-dependent genes, the extent of their requirement remains to be...
Tauchi, Masafumi, Hida, Azumi, Negishi, Takaaki, Katsuoka, Fumiki, Noda, Shuhei, Mimura, Junsei, ...
Occupational and environmental exposure to polycyclic aromatic hydrocarbons (PAHs) has been suggested to provoke inflammatory and/or allergic disorders, including asthma, rhinitis, and dermatitis....
Intrinsic Function of the Aryl Hydrocarbon (Dioxin) Receptor as a Key Factor in Female Reproduction
Baba, Takashi, Mimura, Junsei, Nakamura, Naohito, Harada, Nobuhiro, Yamamoto, Masayuki, Morohashi, Ken-ichirou, ...
Dioxins exert a variety of adverse effects on organisms, including teratogenesis, immunosuppression, tumor promotion, and estrogenic action. Studies using aryl hydrocarbon receptor (AhR)-deficient...
GATA Motifs Regulate Early Hematopoietic Lineage-Specific Expression of the Gata2 Gene
Kobayashi-Osaki, Maki, Ohneda, Osamu, Suzuki, Norio, Minegishi, Naoko, Yokomizo, Tomomasa, Takahashi, Satoru, ...
Kobayashi, Akira, Kang, Moon-Il, Watai, Yoriko, Tong, Kit I., Shibata, Takahiro, Uchida, Koji, ...
The Keap1-Nrf2 system is the major regulatory pathway of cytoprotective gene expression against oxidative and/or electrophilic stresses. Keap1 acts as a stress sensor protein in this system. While...
Yokobayashi, Shihori, Yamamoto, Masayuki, Watanabe, Yoshinori
During mitosis, sister kinetochores attach to microtubules that extend to opposite spindle poles (bipolar attachment) and pull the chromatids apart at anaphase (equational segregation). A...
Yamashita, Akira, Sato, Masamitsu, Fujita, Akiko, Yamamoto, Masayuki, Toda, Takashi
The Ase1/Prc1 proteins constitute a conserved microtubule-associated protein family that is implicated in central spindle formation and cytokinesis. Here we characterize a role for fission yeast...
Nrf2 is a critical regulator of the innate immune response and survival during experimental sepsis
Thimmulappa, Rajesh K., Lee, Hannah, Rangasamy, Tirumalai, Reddy, Sekhar P., Yamamoto, Masayuki, Kensler, Thomas W., ...
Host genetic factors that regulate innate immunity determine susceptibility to sepsis. Disruption of nuclear factor-erythroid 2–related factor 2 (Nrf2), a basic leucine zipper transcription factor...
Tong, Kit I., Katoh, Yasutake, Kusunoki, Hideki, Itoh, Ken, Tanaka, Toshiyuki, Yamamoto, Masayuki
The expression of the phase 2 detoxification enzymes and antioxidant proteins is induced at the transcriptional level by Nrf2 and negatively regulated at the posttranslational level by Keap1 through...
Protective Effects of Medium-Chain Triglycerides on the Liver and Gut in Rats Administered Endotoxin
Kono, Hiroshi, Fujii, Hideki, Asakawa, Masami, Yamamoto, Masayuki, Matsuda, Masanori, Maki, Akira, ...
Combinatorial Gata2 and Sca1 expression defines hematopoietic stem cells in the bone marrow niche
Suzuki, Norio, Ohneda, Osamu, Minegishi, Naoko, Nishikawa, Mitsuo, Ohta, Takayuki, Takahashi, Satoru, ...
The interaction between stem cells and their supportive microenvironment is critical for their maintenance, function, and survival. Whereas hematopoietic stem cells (HSCs) are among the best...
Otori, Muneyoshi, Karashima, Takeshi, Yamamoto, Masayuki
The Deleted in Azoospermia (DAZ) gene family encodes putative translational activators that are required for meiosis and other aspects of gametogenesis in animals. The single Caenorhabditis elegans...
MafG Sumoylation Is Required for Active Transcriptional Repression
Motohashi, Hozumi, Katsuoka, Fumiki, Miyoshi, Chika, Uchimura, Yasuhiro, Saitoh, Hisato, Francastel, Claire, ...
A straightforward mechanism for eliciting transcriptional repression would be to simply block the DNA binding site for activators. Such passive repression is often mediated by transcription factors...
Yamashita, Akira, Yamamoto, Masayuki
During meiotic prophase in the fission yeast Schizosaccharomyces pombe, the nucleus oscillates between the two ends of a cell. This oscillatory nuclear movement is important to promote accurate...
MafB Is Essential for Renal Development and F4/80 Expression in Macrophages
Moriguchi, Takashi, Hamada, Michito, Morito, Naoki, Terunuma, Tsumoru, Hasegawa, Kazuteru, Zhang, Chuan, ...
MafB is a member of the large Maf family of transcription factors that share similar basic region/leucine zipper DNA binding motifs and N-terminal activation domains. Although it is well known that...
Kunitomo, Hirofumi, Higuchi, Toru, Iino, Yuichi, Yamamoto, Masayuki
Schizosaccharomyces pombe ste11 encodes a high-mobility group family transcriptional activator that is pivotal in sexual development. Transcription of ste11 is induced by starvation of nutrients via...
Onodera, Ko, Takahashi, Satoru, Nishimura, Shigeko, Ohta, Jun, Motohashi, Hozumi, Yomogida, Kentaro, ...
Transcription factor GATA-1 is required for the terminal differentiation of both the primitive and definitive erythroid cell lineages, and yet the regulatory mechanisms of GATA-1 itself are not well...
Toya, Mika, Iino, Yuichi, Yamamoto, Masayuki
The fission yeast pob1 gene encodes a protein of 871 amino acids carrying an SH3 domain, a SAM domain, and a PH domain. Gene disruption and construction of a temperature-sensitive pob1 mutant...
Ramos-Gomez, Minerva, Kwak, Mi-Kyoung, Dolan, Patrick M., Itoh, Ken, Yamamoto, Masayuki, Talalay, Paul, ...
Induction of phase 2 enzymes, which neutralize reactive electrophiles and act as indirect antioxidants, appears to be an effective means for achieving protection against a variety of carcinogens in...
Nishimura, Shigeko, Takahashi, Satoru, Kuroha, Takashi, Suwabe, Naruyoshi, Nagasawa, Toshiro, Trainor, Cecelia, ...
A region located at kbp −3.9 to −2.6 5′ to the first hematopoietic exon of the GATA-1 gene is necessary to recapitulate gene expression in both the primitive and definitive erythroid lineages....
Novel WD-Repeat Protein Mip1p Facilitates Function of the Meiotic Regulator Mei2p in Fission Yeast
Shinozaki-Yabana, Satoko, Watanabe, Yoshinori, Yamamoto, Masayuki
In fission yeast, the onset of meiosis is triggered by activation of the RNA-binding protein Mei2p. We screened for a high-copy-number suppressor of the ectopic meiosis induced by expression of an...
Kobayashi, Akira, Yamagiwa, Hironori, Hoshino, Hideto, Muto, Akihiko, Sato, Kazushige, Morita, Masanobu, ...
Bach2 is a B-cell- and neuron-specific transcription repressor that forms heterodimers with the Maf-related oncoproteins. We show here that Bach2 activates transcription by interacting with its novel...
Novel Factor Highly Conserved among Eukaryotes Controls Sexual Development in Fission Yeast
Okazaki, Noriko, Okazaki, Koei, Watanabe, Yoshinori, Kato-Hayashi, Mariko, Yamamoto, Masayuki, Okayama, Hiroto
In the fission yeast Schizosaccharomyces pombe, the onset of sexual development is controlled mainly by two external signals, nutrient starvation and mating pheromone availability. We have isolated a...
Ogawa, Kazuhiro, Sun, Jiying, Taketani, Shigeru, Nakajima, Osamu, Nishitani, Chiaki, Sassa, Shigeru, ...
Heme controls expression of genes involved in the synthesis of globins and heme. The mammalian transcription factor Bach1 functions as a repressor of the Maf recognition element (MARE) by forming...
In vivo requirements for GATA-1 functional domains during primitive and definitive erythropoiesis
Shimizu, Ritsuko, Takahashi, Satoru, Ohneda, Kinuko, Engel, James Douglas, Yamamoto, Masayuki
GATA-1 is a transcription factor essential for erythroid/megakaryocytic cell differentiation. To investigate the contribution of individual domains of GATA-1 to its activity, transgenic mice...
An embryonic/fetal β-type globin gene repressor contains a nuclear receptor TR2/TR4 heterodimer
Tanabe, Osamu, Katsuoka, Fumiki, Campbell, Andrew D., Song, Weimin, Yamamoto, Masayuki, Tanimoto, Keiji, ...
We recently described an erythroid ε-globin gene repressor activity, which we named DRED (direct repeat erythroid-definitive). We show that DRED binds with high affinity to DR1 sites in the human...
Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene
Sun, Jiying, Hoshino, Hideto, Takaku, Kazuaki, Nakajima, Osamu, Muto, Akihiko, Suzuki, Hiroshi, ...
Heme oxygenase-1 (HO-1) protects cells from various insults including oxidative stress. Transcriptional activators, including the Nrf2/Maf heterodimer, have been the focus of studies on the inducible...
Dinkova-Kostova, Albena T., Holtzclaw, W. David, Cole, Robert N., Itoh, Ken, Wakabayashi, Nobunao, Katoh, Yasutake, ...
Coordinate induction of phase 2 proteins and elevation of glutathione protect cells against the toxic and carcinogenic effects of electrophiles and oxidants. All inducers react covalently with thiols...
Kwak, Mi-Kyoung, Itoh, Ken, Yamamoto, Masayuki, Kensler, Thomas W.
Induction of phase 2 enzymes, which neutralize reactive electrophiles and act as indirect antioxidants, is an important mechanism for protection against carcinogenesis. The transcription factor Nrf2,...
Higuchi, Toru, Watanabe, Yoshinori, Yamamoto, Masayuki
Protein kinase A (PKAi a cyclic AMP-dependent protein kinase) negatively regulates sexual development and gluconeogenesis in fission yeast by suppressing the transcription of ste11 required for the...
Ishizaki, Kimitsune, Shimizu-Ueda, Yuu, Okada, Sachiko, Yamamoto, Masayuki, Fujisawa, Masaki, Yamato, Katsuyuki T., ...
Sex of the liverwort Marchantia polymorpha is determined by the sex chromosomes Y and X, in male and female plant, respectively. Approximately half of the Y chromosome is made up of unique repeat...
Katsuoka, Fumiki, Motohashi, Hozumi, Tamagawa, Yuna, Kure, Shigeo, Igarashi, Kazuhiko, Engel, James Douglas, ...
The small Maf proteins form heterodimers with CNC and Bach family proteins to elicit transcriptional responses from Maf recognition elements (MAREs). We previously reported germ line-targeted...
HLF/HIF-2α is a key factor in retinopathy of prematurity in association with erythropoietin
Morita, Masanobu, Ohneda, Osamu, Yamashita, Toshiharu, Takahashi, Satoru, Suzuki, Norio, Nakajima, Osamu, ...
An HLF (HIF-1α-like factor)/HIF-2α-knockout mouse is embryonic lethal, preventing investigation of HLF function in adult mice. To investigate the role of HLF in adult pathological angiogenesis, we...
Functional Analysis of Basic Transcription Element Binding Protein by Gene Targeting Technology
Morita, Masanobu, Kobayashi, Akira, Yamashita, Toshiharu, Shimanuki, Tomomasa, Nakajima, Osamu, Takahashi, Satoru, ...
Basic transcription element binding protein (BTEB) is a transcription factor with a characteristic zinc finger motif and is most remarkably enhanced by thyroid hormone T3 treatment (R. J. Denver et...
Yokobayashi, Shihori, Yamamoto, Masayuki, Watanabe, Yoshinori
During mitosis, sister kinetochores attach to microtubules that extend to opposite spindle poles (bipolar attachment) and pull the chromatids apart at anaphase (equational segregation). A...
Distinct response to dioxin in an arylhydrocarbon receptor (AHR)-humanized mouse
Moriguchi, Takashi, Motohashi, Hozumi, Hosoya, Tomonori, Nakajima, Osamu, Takahashi, Satoru, Ohsako, Seiichiroh, ...
There are large inter- and intraspecies differences in susceptibility to dioxin-induced toxicities. A critical question in risk assessment of dioxin and related compounds is whether humans are...
Matsuo, Tomohiko, Kubo, Yoshiya, Watanabe, Yoshinori, Yamamoto, Masayuki
The TOR protein is a phosphoinositide kinase-related kinase widely conserved among eukaryotes. Fission yeast tor1 encodes an ortholog of TOR, which is required for sexual development and growth under...
Mito, Yoshiko, Sugimoto, Asako, Yamamoto, Masayuki
Cohesin, which mediates sister chromatid cohesion, is composed of four subunits, named Scc1/Rad21, Scc3, Smc1, and Smc3 in yeast. Caenorhabditis elegans has a single homolog for each of Scc3, Smc1,...
Shimada, Tadayuki, Yamashita, Akira, Yamamoto, Masayuki
Fission yeast Mei2p is an RNA-binding protein essential for induction of both premeiotic DNA synthesis and first meiotic division. Mei2p forms a dot structure at an apparently fixed position in the...
Hirota, Kouji, Tanaka, Kayoko, Ohta, Kunihiro, Yamamoto, Masayuki
Fission yeast Cdc42p, a small GTPase of the Rho family, is essential for cell proliferation and maintenance of the rod-like cell morphology. Scd1/Ral1p is a GDP-GTP exchange factor (GEF) for Cdc42p....
One enhancer mediates mafK transcriptional activation in both hematopoietic and cardiac muscle cells
Katsuoka, Fumiki, Motohashi, Hozumi, Onodera, Ko, Suwabe, Naruyoshi, Engel, James Douglas, Yamamoto, Masayuki
Members of the small Maf family of transcription factors play important roles in hematopoiesis. Using transgenic assays, we discovered a tissue-specific enhancer 3′ to the mafK gene. This enhancer...
Rec8 cleavage by separase is required for meiotic nuclear divisions in fission yeast
Kitajima, Tomoya S., Miyazaki, Yousuke, Yamamoto, Masayuki, Watanabe, Yoshinori
Sister chromatid cohesion in meiosis is established by cohesin complexes, including the Rec8 subunit. During meiosis I, sister chromatid cohesion is destroyed along the chromosome arms to release...
Self-Association of Gata1 Enhances Transcriptional Activity In Vivo in Zebra Fish Embryos
Nishikawa, Keizo, Kobayashi, Makoto, Masumi, Atsuko, Lyons, Susan E., Weinstein, Brant M., Liu, P. Paul, ...
Gata1 is a prototype transcription factor that regulates hematopoiesis, yet the molecular mechanisms by which Gata1 transactivates its target genes in vivo remain unclear. We previously showed, in...
Antioxidants Enhance Mammalian Proteasome Expression through the Keap1-Nrf2 Signaling Pathway
Kwak, Mi-Kyoung, Wakabayashi, Nobunao, Greenlaw, Jennifer L., Yamamoto, Masayuki, Kensler, Thomas W.
Proteasomes degrade damaged proteins formed during oxidative stress, thereby promoting cell survival. Neurodegenerative and other age-related disorders are associated with reduced proteasome...
Proplatelet formation of megakaryocytes is triggered by autocrine-synthesized estradiol
Nagata, Yuka, Yoshikawa, Jun, Hashimoto, Atsushi, Yamamoto, Masayuki, Payne, Anita H., Todokoro, Kazuo
A matured megakaryocyte releases thousands of platelets through a drastic morphological change, proplatelet formation (PPF). The megakaryocyte/erythrocyte-specific transcription factor, p45 NF-E2, is...
Itoh, Ken, Mochizuki, Mie, Ishii, Yukio, Ishii, Tetsuro, Shibata, Takahiro, Kawamoto, Yoshiyuki, ...
Activated macrophages express high levels of Nrf2, a transcription factor that positively regulates the gene expression of antioxidant and detoxication enzymes. In this study, we examined how Nrf2...
Perinatal synthetic lethality and hematopoietic defects in compound mafG::mafK mutant mice
Onodera, Ko, Shavit, Jordan A., Motohashi, Hozumi, Yamamoto, Masayuki, Engel, James Douglas
Prior studies exploring the mechanisms controlling erythroid gene regulation implicated MARE (Maf recognition element) cis–elements as crucial to the transcriptional activity of many erythroid...
Itoh, Ken, Wakabayashi, Nobunao, Katoh, Yasutake, Ishii, Tetsuro, Igarashi, Kazuhiko, Engel, James Douglas, ...
Transcription factor Nrf2 is essential for the antioxidant responsive element (ARE)-mediated induction of phase II detoxifying and oxidative stress enzyme genes. Detailed analysis of differential...
Impaired megakaryopoiesis and behavioral defects in mafG-null mutant mice
Shavit, Jordan A., Motohashi, Hozumi, Onodera, Ko, Akasaka, Jun-etsu, Yamamoto, Masayuki, Engel, James Douglas
The small Maf proteins (MafG, MafK, and MafF), which serve as heterodimeric partner molecules of CNC family proteins for binding in vitro to MARE sites, have been implicated in the regulation of both...
Wakabayashi, Nobunao, Dinkova-Kostova, Albena T., Holtzclaw, W. David, Kang, Moon-Il, Kobayashi, Akira, Yamamoto, Masayuki, ...
Induction of a family of phase 2 genes encoding for proteins that protect against the damage of electrophiles and reactive oxygen intermediates is potentially a major strategy for reducing the risk...
Kang, Moon-Il, Kobayashi, Akira, Wakabayashi, Nobunao, Kim, Sang-Geon, Yamamoto, Masayuki
Transcription factor Nrf2 regulates basal and inducible expression of phase 2 proteins that protect animal cells against the toxic effects of electrophiles and oxidants. Under basal conditions, Nrf2...
Negative control for the initiation of meiosis in Schizosaccharomyces pombe
Iino, Yuichi, Yamamoto, Masayuki
Temperature-sensitive mutants of the pat1 gene of Schizosaccharomyces pombe are able to produce poorly viable spores from the haploid state without the requirement for nitrogen starvation if exposed...
Motohashi, Hozumi, Katsuoka, Fumiki, Engel, James Douglas, Yamamoto, Masayuki
The small Maf proteins, MafF, MafG, and MafK, possess a leucine zipper (Zip) domain that is required for homodimer or heterodimer complex formation with other bZip transcription factors. In this...
Kobayashi, Akira, Kang, Moon-Il, Okawa, Hiromi, Ohtsuji, Makiko, Zenke, Yukari, Chiba, Tomoki, ...
Transcription factor Nrf2 is a major regulator of genes encoding phase 2 detoxifying enzymes and antioxidant stress proteins in response to electrophilic agents and oxidative stress. In the absence...
Inoue, Takao, Sugimoto, Asako, Suzuki, Yuka, Yamamoto, Masayuki, Tsujimoto, Masafumi, Inoue, Keizo, ...
Type II platelet-activating factor-acetylhydrolase [PAF-AH (II)] is an N-myristoylated enzyme that contains a lipase/esterase catalytic motif and selectively hydrolyzes the sn-2 acetyl ester of PAF...
Genetic ablation of Nrf2 enhances susceptibility to cigarette smoke–induced emphysema in mice
Rangasamy, Tirumalai, Cho, Chung Y., Thimmulappa, Rajesh K., Zhen, Lijie, Srisuma, Sorachai S., Kensler, Thomas W., ...
Although inflammation and protease/antiprotease imbalance have been postulated to be critical in cigarette smoke–induced (CS-induced) emphysema, oxidative stress has been suspected to play an...
Khandekar, Melin, Suzuki, Norio, Lewton, Jon, Yamamoto, Masayuki, Engel, James Douglas
Transcription factor GATA-2 is expressed in a complex temporally and tissue-specific pattern within the developing embryo. Loss-of-function studies in the mouse showed that GATA-2 activity is first...
Leukemogenesis Caused by Incapacitated GATA-1 Function
Shimizu, Ritsuko, Kuroha, Takashi, Ohneda, Osamu, Pan, Xiaoqing, Ohneda, Kinuko, Takahashi, Satoru, ...
GATA-1 is essential for the development of erythroid and megakaryocytic lineages. We found that GATA-1 gene knockdown female (GATA-1.05/X) mice frequently develop a hematopoietic disorder resembling...
Molecular Determinants of NOTCH4 Transcription in Vascular Endothelium
Wu, Jing, Iwata, Fumiko, Grass, Jeffrey A., Osborne, Cameron S., Elnitski, Laura, Fraser, Peter, ...
The process whereby the primitive vascular network develops into the mature vasculature, known as angiogenic vascular remodeling, is controlled by the Notch signaling pathway. Of the two mammalian...
GATA1 Function, a Paradigm for Transcription Factors in Hematopoiesis
Ferreira, Rita, Ohneda, Kinuko, Yamamoto, Masayuki, Philipsen, Sjaak
Yamashita, Akira, Sato, Masamitsu, Fujita, Akiko, Yamamoto, Masayuki, Toda, Takashi
The Ase1/Prc1 proteins constitute a conserved microtubule-associated protein family that is implicated in central spindle formation and cytokinesis. Here we characterize a role for fission yeast...
Kelly, Vincent P., Suzuki, Takafumi, Nakajima, Osamu, Arai, Tsuyoshi, Tamai, Yoshitaka, Takahashi, Satoru, ...
Appropriate expression of the selenocysteine tRNA (tRNASec) gene is necessary for the production of an entire family of selenoprotein enzymes. This study investigates the consequence of disrupting an...
MafA Is a Key Regulator of Glucose-Stimulated Insulin Secretion
Zhang, Chuan, Moriguchi, Takashi, Kajihara, Miwako, Esaki, Ritsuko, Harada, Ayako, Shimohata, Homare, ...
MafA is a transcription factor that binds to the promoter in the insulin gene and has been postulated to regulate insulin transcription in response to serum glucose levels, but there is no current in...
Mating pheromone-like diffusible factor released by Schizosaccharomyces pombe
Fukui, Yasuhisa, Kaziro, Yoshito, Yamamoto, Masayuki
We demonstrate that a diffusible factor is secreted by h− cells of the fission yeast Schizosaccharomyces pombe, whose mating pheromones have not been described. This factor, tentatively named the...
Suzuki, Takafumi, Takagi, Yaeko, Osanai, Hitoshi, Li, Li, Takeuchi, Miki, Katoh, Yasutake, ...
Pi class GSTs (glutathione S-transferases) are a member of the vertebrate GST family of proteins that catalyse the conjugation of GSH to electrophilic compounds. The expression of Pi class GST genes...
GATA Motifs Regulate Early Hematopoietic Lineage-Specific Expression of the Gata2 Gene
Kobayashi-Osaki, Maki, Ohneda, Osamu, Suzuki, Norio, Minegishi, Naoko, Yokomizo, Tomomasa, Takahashi, Satoru, ...
Transcription factor GATA-2 is essential for definitive hematopoiesis, which developmentally emerges from the para-aortic splanchnopleura (P-Sp). The expression of a green fluorescent protein (GFP)...
Chanas, Simon A, Jiang, Qing, McMahon, Michael, McWalter, Gail K, McLellan, Lesley I, Elcombe, Clifford R, ...
Mice that lack the Nrf2 basic-region leucine-zipper transcription factor are more sensitive than wild-type (WT) animals to the cytotoxic and genotoxic effects of foreign chemicals and oxidants. To...
Nioi, Paul, McMahon, Michael, Itoh, Ken, Yamamoto, Masayuki, Hayes, John D
NQO1 [NAD(P)H:quinone oxidoreductase 1] has an integral role in cellular responses to oxidative stress. The expression of NQO1 is up-regulated in the mouse following challenge with electrophilic...
Katsuoka, Fumiki, Motohashi, Hozumi, Ishii, Tetsuro, Aburatani, Hiroyuki, Engel, James Douglas, Yamamoto, Masayuki
While small Maf proteins have been suggested to be essential for the Nrf2-mediated activation of antioxidant response element (ARE)-dependent genes, the extent of their requirement remains to be...
Tauchi, Masafumi, Hida, Azumi, Negishi, Takaaki, Katsuoka, Fumiki, Noda, Shuhei, Mimura, Junsei, ...
Occupational and environmental exposure to polycyclic aromatic hydrocarbons (PAHs) has been suggested to provoke inflammatory and/or allergic disorders, including asthma, rhinitis, and dermatitis....
Intrinsic Function of the Aryl Hydrocarbon (Dioxin) Receptor as a Key Factor in Female Reproduction
Baba, Takashi, Mimura, Junsei, Nakamura, Naohito, Harada, Nobuhiro, Yamamoto, Masayuki, Morohashi, Ken-ichirou, ...
Dioxins exert a variety of adverse effects on organisms, including teratogenesis, immunosuppression, tumor promotion, and estrogenic action. Studies using aryl hydrocarbon receptor (AhR)-deficient...
GATA Motifs Regulate Early Hematopoietic Lineage-Specific Expression of the Gata2 Gene
Kobayashi-Osaki, Maki, Ohneda, Osamu, Suzuki, Norio, Minegishi, Naoko, Yokomizo, Tomomasa, Takahashi, Satoru, ...
Kobayashi, Akira, Kang, Moon-Il, Watai, Yoriko, Tong, Kit I., Shibata, Takahiro, Uchida, Koji, ...
The Keap1-Nrf2 system is the major regulatory pathway of cytoprotective gene expression against oxidative and/or electrophilic stresses. Keap1 acts as a stress sensor protein in this system. While...
Combinatorial Gata2 and Sca1 expression defines hematopoietic stem cells in the bone marrow niche
Suzuki, Norio, Ohneda, Osamu, Minegishi, Naoko, Nishikawa, Mitsuo, Ohta, Takayuki, Takahashi, Satoru, ...
The interaction between stem cells and their supportive microenvironment is critical for their maintenance, function, and survival. Whereas hematopoietic stem cells (HSCs) are among the best...
Nrf2 is a critical regulator of the innate immune response and survival during experimental sepsis
Thimmulappa, Rajesh K., Lee, Hannah, Rangasamy, Tirumalai, Reddy, Sekhar P., Yamamoto, Masayuki, Kensler, Thomas W., ...
Host genetic factors that regulate innate immunity determine susceptibility to sepsis. Disruption of nuclear factor-erythroid 2–related factor 2 (Nrf2), a basic leucine zipper transcription factor...
Tong, Kit I., Katoh, Yasutake, Kusunoki, Hideki, Itoh, Ken, Tanaka, Toshiyuki, Yamamoto, Masayuki
The expression of the phase 2 detoxification enzymes and antioxidant proteins is induced at the transcriptional level by Nrf2 and negatively regulated at the posttranslational level by Keap1 through...
Otori, Muneyoshi, Karashima, Takeshi, Yamamoto, Masayuki
The Deleted in Azoospermia (DAZ) gene family encodes putative translational activators that are required for meiosis and other aspects of gametogenesis in animals. The single Caenorhabditis elegans...
MafG Sumoylation Is Required for Active Transcriptional Repression
Motohashi, Hozumi, Katsuoka, Fumiki, Miyoshi, Chika, Uchimura, Yasuhiro, Saitoh, Hisato, Francastel, Claire, ...
A straightforward mechanism for eliciting transcriptional repression would be to simply block the DNA binding site for activators. Such passive repression is often mediated by transcription factors...
Protective Effects of Medium-Chain Triglycerides on the Liver and Gut in Rats Administered Endotoxin
Kono, Hiroshi, Fujii, Hideki, Asakawa, Masami, Yamamoto, Masayuki, Matsuda, Masanori, Maki, Akira, ...
Yamashita, Akira, Yamamoto, Masayuki
During meiotic prophase in the fission yeast Schizosaccharomyces pombe, the nucleus oscillates between the two ends of a cell. This oscillatory nuclear movement is important to promote accurate...
MafB Is Essential for Renal Development and F4/80 Expression in Macrophages
Moriguchi, Takashi, Hamada, Michito, Morito, Naoki, Terunuma, Tsumoru, Hasegawa, Kazuteru, Zhang, Chuan, ...
MafB is a member of the large Maf family of transcription factors that share similar basic region/leucine zipper DNA binding motifs and N-terminal activation domains. Although it is well known that...
Mukai, Harumi Y., Motohashi, Hozumi, Ohneda, Osamu, Suzuki, Norio, Nagano, Masumi, Yamamoto, Masayuki
The nuclear proto-oncogene c-myb plays crucial roles in the growth, survival, and differentiation of hematopoietic cells. We established three lines of erythropoietin receptor-transgenic mice and...
BRG1 Interacts with Nrf2 To Selectively Mediate HO-1 Induction in Response to Oxidative Stress▿
Zhang, Jianyong, Ohta, Tsutomu, Maruyama, Atsushi, Hosoya, Tomonori, Nishikawa, Keizo, Maher, Jonathan M., ...
NF-E2-related factor 2 (Nrf2) regulates antioxidant-responsive element-mediated induction of cytoprotective genes in response to oxidative stress. The purpose of this study was to determine the role...
Zhang, Yiguo, Crouch, Dorothy H., Yamamoto, Masayuki, Hayes, John D.
Nrf1 (nuclear factor-erythroid 2 p45 subunit-related factor 1) and Nrf2 regulate ARE (antioxidant response element)-driven genes. At its N-terminal end, Nrf1 contains 155 additional amino acids that...
Wilms' tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA
Horiuchi, Keiko, Umetani, Michihisa, Minami, Takashi, Okayama, Hiroto, Takada, Shinji, Yamamoto, Masayuki, ...
Wilms' tumor 1-associating protein (WTAP) has been reported to be a ubiquitously expressed nuclear protein. Although a relation to splicing factors has been postulated, its actual physiological...
Matsuo, Tomohiko, Otsubo, Yoko, Urano, Jun, Tamanoi, Fuyuhiko, Yamamoto, Masayuki
Fission yeast has two TOR (target of rapamycin) kinases, namely Tor1 and Tor2. Tor1 is required for survival under stressed conditions, proper G1 arrest, and sexual development. In contrast, Tor2 is...
Urano, Jun, Sato, Tatsuhiro, Matsuo, Tomohiko, Otsubo, Yoko, Yamamoto, Masayuki, Tamanoi, Fuyuhiko
Rheb is a unique member of the Ras superfamily GTP-binding proteins. We as well as others previously have shown that Rheb is a critical component of the TSC/TOR signaling pathway. In fission yeast,...
Padmanabhan, Balasundaram, Scharlock, Maria, Tong, Kit I., Nakamura, Yoshihiro, Kang, Moon-Il, Kobayashi, Akira, ...
Keap1-DC (Kelch/double-glycine repeat and C-terminal region) of mouse Keap1 has been overexpressed in E. coli, purified and crystallized using the vapour-diffusion method.
Yamato, Katsuyuki T., Ishizaki, Kimitsune, Fujisawa, Masaki, Okada, Sachiko, Nakayama, Shigeki, Fujishita, Mariko, ...
Y chromosomes are different from other chromosomes because of a lack of recombination. Until now, complete sequence information of Y chromosomes has been available only for some primates, although...
Characterization of GATA-1+ hemangioblastic cells in the mouse embryo
Yokomizo, Tomomasa, Takahashi, Satoru, Mochizuki, Naomi, Kuroha, Takashi, Ema, Masatsugu, Wakamatsu, Asami, ...
Hemangioblasts are thought to be one of the sources of hematopoietic progenitors, yet little is known about their localization and fate in the mouse embryo. We show here that a subset of cells...
Nrf2 Neh5 domain is differentially utilized in the transactivation of cytoprotective genes
Zhang, Jianyong, Hosoya, Tomonori, Maruyama, Atsushi, Nishikawa, Keizo, Maher, Jonathan M., Ohta, Tsutomu, ...
The transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) contains two transcription activation domains, Neh4 (Nrf2 ECH homology 4) and Neh5, which co-ordinately regulate...
NRF2 Modulates Aryl Hydrocarbon Receptor Signaling: Influence on Adipogenesis▿ †
Shin, Soona, Wakabayashi, Nobunao, Misra, Vikas, Biswal, Shyam, Lee, Gum Hwa, Agoston, Elin S., ...
The NF-E2 p45-related factor 2 (NRF2) and the aryl hydrocarbon receptor (AHR) are transcription factors controlling pathways modulating xenobiotic metabolism. AHR has recently been shown to affect...
Tong, Kit I., Padmanabhan, Balasundaram, Kobayashi, Akira, Shang, Chengwei, Hirotsu, Yosuke, Yokoyama, Shigeyuki, ...
Nrf2 is the regulator of the oxidative/electrophilic stress response. Its turnover is maintained by Keap1-mediated proteasomal degradation via a two-site substrate recognition mechanism in which two...
Disruption of Nrf2 enhances susceptibility to severe airway inflammation and asthma in mice
Rangasamy, Tirumalai, Guo, Jia, Mitzner, Wayne A., Roman, Jessica, Singh, Anju, Fryer, Allison D., ...
Oxidative stress has been postulated to play an important role in the pathogenesis of asthma; although a defect in antioxidant responses has been speculated to exacerbate asthma severity, this has...
Ohata, Hisayuki, Tanaka, Ken-ichi, Maeyama, Naoto, Yamamoto, Masayuki, Momose, Kazutaka
Local increases in the intracellular Ca2+ concentration ([Ca2+]i) in several regions within the bovine lens epithelial cell during application of mechanical stress were clearly visualized in the...
Essential and Instructive Roles of GATA Factors in Eosinophil Development
Hirasawa, Ryutaro, Shimizu, Ritsuko, Takahashi, Satoru, Osawa, Mitsujiro, Takayanagi, Shu, Kato, Yuko, ...
GATA transcription factors are major regulators of hematopoietic and immune system. Among GATA factors, GATA-1, GATA-2, and GATA-3 play crucial roles in the development of erythroid cells,...
The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription
Tanabe, Osamu, Shen, Yannan, Liu, Qinghui, Campbell, Andrew D., Kuroha, Takashi, Yamamoto, Masayuki, ...
When the orphan nuclear receptors TR2 and TR4, the DNA-binding subunits of the DRED repressor complex, are forcibly expressed in erythroid cells of transgenic mice, embryos exhibit a transient...
Embryonic and fetal β-globin gene repression by the orphan nuclear receptors, TR2 and TR4
Tanabe, Osamu, McPhee, David, Kobayashi, Shoko, Shen, Yannan, Brandt, William, Jiang, Xia, ...
The TR2 and TR4 orphan nuclear receptors comprise the DNA-binding core of direct repeat erythroid definitive, a protein complex that binds to direct repeat elements in the embryonic and fetal β-type...
Kitayama, Chikako, Sugimoto, Asako, Yamamoto, Masayuki
We cloned the myo2 gene of Schizosaccharomyces pombe, which encodes a type II myosin heavy chain, by virtue of its ability to promote diploidization in fission yeast cells. The myo2 gene encodes...
Yamashita, Toshiharu, Ohneda, Osamu, Nagano, Masumi, Iemitsu, Motoyuki, Makino, Yuichi, Tanaka, Hirotoshi, ...
Hypoxia-inducible factors (HIFs) are crucial for oxygen homeostasis during both embryonic development and postnatal life. Here we show that a novel HIF family basic helix-loop-helix (bHLH) PAS...
Zhang, Yiguo, Lucocq, John M., Yamamoto, Masayuki, Hayes, John D.
Nrf1 (nuclear factor-erythroid 2 p45 subunit-related factor 1) is negatively controlled by its NTD (N-terminal domain) that lies between amino acids 1 and 124. This domain contains a leucine-rich...
Deficiency in Nrf2-GSH Signaling Impairs Type II Cell Growth and Enhances Sensitivity to Oxidants
Reddy, Narsa M., Kleeberger, Steven R., Cho, Hye-Youn, Yamamoto, Masayuki, Kensler, Thomas W., Biswal, Shyam, ...
Redox imbalance has been implicated in the pathogenesis of many acute and chronic lung diseases. The b-Zip transcription factor Nrf2 acts via an antioxidant/electrophilic response element to regulate...
Padmanabhan, Balasundaram, Tong, Kit I., Kobayashi, Akira, Yamamoto, Masayuki, Yokoyama, Shigeyuki
The structure of mouse Keap1-DC complexed with the DLG motif peptide of Nrf2 transcription factor was determined at 1.9 Å resolution. The structure showed that the peptide binds to Keap1-DC at the...
Physiological Significance of Reactive Cysteine Residues of Keap1 in Determining Nrf2 Activity▿
Yamamoto, Tae, Suzuki, Takafumi, Kobayashi, Akira, Wakabayashi, Junko, Maher, Jon, Motohashi, Hozumi, ...
Keap1 and Cul3 constitute a unique ubiquitin E3 ligase that degrades Nrf2, a key activator of cytoprotective genes. Upon exposure to oxidants/electrophiles, the enzymatic activity of this ligase...
Nrf2 mediates cancer protection but not prolongevity induced by caloric restriction
Pearson, Kevin J., Lewis, Kaitlyn N., Price, Nathan L., Chang, Joy W., Perez, Evelyn, Cascajo, Maria Victoria, ...
Caloric restriction (CR) is the most potent intervention known to both protect against carcinogenesis and extend lifespan in laboratory animals. A variety of anticarcinogens and CR mimetics induce...
Genetic and Pharmacologic Evidence Links Oxidative Stress to Ventilator-induced Lung Injury in Mice
Papaiahgari, Srinivas, Yerrapureddy, Adi, Reddy, Swetha R., Reddy, Narsa M., Dodd-O, Jeffery M., Crow, Michael T., ...
Rationale: Mechanical ventilation (MV) is an indispensable therapy for critically ill patients with acute lung injury and the adult respiratory distress syndrome. However, the mechanisms by which...
Minute ampullary carcinoid tumor with lymph node metastases: a case report and review of literature
Senda, Eri, Fujimoto, Koji, Ohnishi, Katsuhiro, Higashida, Akihiro, Ashida, Cho, Okutani, Toshio, ...
Singh, Anju, Rangasamy, Tirumalai, Thimmulappa, Rajesh K., Lee, Hannah, Osburn, William O., Brigelius-Flohé, Regina, ...
Disruption of NF-E2–related factor (Nrf2), a redox-sensitive basic leucine zipper transcription factor, causes early-onset and more severe emphysema due to chronic cigarette smoke. Nrf2 determines...
Shibata, Tatsuhiro, Ohta, Tsutomu, Tong, Kit I., Kokubu, Akiko, Odogawa, Reiko, Tsuta, Koji, ...
The nuclear factor E2-related factor 2 (Nrf2) is a master transcriptional activator of genes encoding numerous cytoprotective enzymes that are induced in response to environmental and endogenously...
Der Verfasser hat die Milch von 37 Fallen mit Stauungsmastitis bakteriologisch untersucht und unter verschiedenen Bedingungen die Veranderungen der Bakterienverhaltnisse beobachtet. Weiter hat er die...
Brandt, William, Khandekar, Melin, Suzuki, Norio, Yamamoto, Masayuki, Lim, Kim-Chew, Engel, James Douglas
Transcription factor GATA-2 is vital for both hematopoietic progenitor cell function and urogenital patterning. Transgenic mapping studies have shown that the hematopoietic and urogenital enhancers...
Kadri, Zahra, Shimizu, Ritsuko, Ohneda, Osamu, Maouche-Chretien, Leila, Gisselbrecht, Sylvie, Yamamoto, Masayuki, ...
Cell differentiation is often coupled with cell cycle arrest. Here, we show that direct binding of the erythroid transcription factor GATA-1 to the retinoblastoma protein and the pRb/E2F...
Kobayashi, Makoto, Li, Li, Iwamoto, Noriko, Nakajima-Takagi, Yaeko, Kaneko, Hiroshi, Nakayama, Yuko, ...
Animals have evolved defense systems for surviving in a chemically diverse environment. Such systems should demonstrate plasticity, such as adaptive immunity, enabling a response to even unknown...
Sussan, Thomas E., Rangasamy, Tirumalai, Blake, David J., Malhotra, Deepti, El-Haddad, Hazim, Bedja, Djahida, ...
Chronic obstructive pulmonary disease (COPD), which comprises emphysema and chronic bronchitis resulting from prolonged exposure to cigarette smoke (CS), is a major public health burden with no...
Differential Contribution of the Gata1 Gene Hematopoietic Enhancer to Erythroid Differentiation▿ †
Suzuki, Mikiko, Moriguchi, Takashi, Ohneda, Kinuko, Yamamoto, Masayuki
GATA1 is a key regulator of erythroid cell differentiation. To examine how Gata1 gene expression is regulated in a stage-specific manner, transgenic mouse lines expressing green fluorescent protein...
Sato, Masamitsu, Okada, Naoyuki, Kakui, Yasutaka, Yamamoto, Masayuki, Yoshida, Minoru, Toda, Takashi
Ran GTPase activates several target molecules to induce microtubule formation around the chromosomes and centrosomes. In fission yeast, in which the nuclear envelope does not break down during...