Reichardt, Peter, Hogendoorn, Pancras C. W, Tamborini, Elena, Loda, Massimo, Gronchi, Alessandro, Poveda, Andres, ...
Fatty Acid Synthase: A Metabolic Enzyme and Candidate Oncogene in Prostate Cancer (2009)
Migita, Toshiro, Ruiz, Stacey, Fornari, Alessandro, Fiorentino, Michelangelo, Priolo, Carmen, Zadra, Giorgia, ...
Background Overexpression of the fatty acid synthase (FASN) gene has been implicated in prostate carcinogenesis. We sought to directly assess the oncogenic potential of FASN. Methods We used...
Potential role of miR-9 and miR-223 in recurrent ovarian cancer (2008)
Laios, Alexandros, O'Toole, Sharon, Flavin, Richard, Martin, Cara, Kelly, Lynne, Ring, Martina, ...
Abstract Background MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression by binding to target mRNAs. miRNAs have not been comprehensively studied in recurrent ovarian...
Simona Nanni, Carmen Priolo, Annalisa Grasselli, Roberta Merola, Fabiola Moretti, Michele Gallucci, ...
The histopathologic and molecular heterogeneity of prostate cancer and the limited availability of human tumor tissue make unraveling the mechanisms of prostate carcinogenesis a challenging task. Our...
Febbo, Phillip G, Mulligan, Mike G, Slonina, David A, Stegmaier, Kimberly, Di Vizio, Dolores, Martinez, Paul R, ...
Abstract Background The biomedical literature is a rich source of associative information but too vast for complete manual review. We have developed an automated method of literature interrogation...
SUPPLEMENTARY INFORMATION Multi-class Cancer Diagnosis Using Tumor Gene Expression Signatures (2007)
Sridhar Ramaswamy, Pablo Tamayo, Ryan Rifkin, Sayan Mukherjee, Hsiang Yeang, ...
This document provides supplementary information and details not included in the paper "Multiclass Cancer Diagnosis Using Tumor Gene Expression Signatures." Other sources of information can...
Di Vizio, Dolores, Sotgia, Federica, Williams, Terence M, Hassan, Ghada S, Capozza, Franco, Frank, Philippe G, ...
Prostate cancer is the second leading cause of cancer-related deaths in men. Fatty acid synthase (FASN) is normally upregulated during human prostate cancer onset and metastatic progression and its...
Corrigendum: High-throughput oncogene mutation profiling in human cancer. (2007)
Thomas, Roman K, Baker, Alissa C, Debiasi, Ralph M, Winckler, Wendy, Laframboise, Thomas, Lin, William M, ...
Systematic efforts are underway to decipher the genetic changes associated with tumor initiation and progression1, 2. However, widespread clinical application of this information is hampered by an...
High-throughput oncogene mutation profiling in human cancer (2007)
Thomas, Roman K., Baker, Alissa C., Debiasi, Ralph M., Winckler, Wendy, Laframboise, Thomas, Lin, William M., ...
Bmc Genomics, Michael G Mulligan, David A Slonina, Kimberly Stegmaier, Dolores Di Vizio, Paul R Martinez, ...
This Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Literature Lab: a method of automated...
Intermediate basal cells of the prostate: In vitro and in vivo characterization (2003)
Garraway, Levi, Lin, Douglas, Signoretti, Sabina, Waltregny, David, Dilks, James, Bhattacharya, Nandita, ...
BACKGROUND. Progenitor cells within the prostate basal layer may play important roles in differentiation and carcinogenesis; however, prostate stem cell populations remain uncharacterized. METHODS....
Androgen Regulation of p27 in the Normal and Neoplastic Prostate (2003)
Hypothesis and rationale: The hypothesis of the proposal is that the cyclin-dependent kinase inhibitor p27 is regulated by androgens in both normal and neoplastic prostate. We also hypothesized that...
Abstract Turnover of several regulatory proteins results from targeted destruction via ubiquitination and subsequent degradation through the proteosome. The timely and irreversible degradation of...
Lindeman, Neal, Waltregny, David, Signoretti, Sabina, Loda, Massimo
Studying tissue-based gene expression in different cell populations often requires immunohistochemistry-guided microdissection. However, mRNA degradation occurs during long staining procedures. We...
Waltregny, David, Leav, Irwin, Signoretti, Sabina, Soung, Peggy, Lin, Douglas, Merk, Frederick, ...
Androgens control both growth and differentiation of the normal prostate gland. However, the mechanisms by which androgens act upon the cell cycle machinery to regulate these two fundamental...
p63 is a prostate basal cell marker and is required for prostate development. (2000)
Signoretti, Sabina, Waltregny, David, Dilks, James, Isaac, Beth, Lin, Douglas, Garraway, Levi, ...
The p53 homologue p63 encodes for different isotypes able to either transactivate p53 reporter genes (TAp63) or act as p53-dominant-negatives (DeltaNp63). p63 is expressed in the basal cells of many...
Her-2-neu Expression and Progression Toward Androgen Independence in Human Prostate Cancer (2000)
Signoretti, Sabina, Montironi, Rodolfo, Manola, Judith, Altimari, Annalisa, Tam, Carmen, Bubley, Glenn, ...
Background: Human prostate cancers are initially androgen dependent but ultimately become androgen independent. Overexpression of the Her-2-neu receptor tyrosine kinase has been associated with the...
Androgen Regulation of p27 in the Normal and Neoplastic Prostate (1998)
The cyclin dependent kinase inhibitor (CKI) p27 is a negative regulator of the cell cycle and a potential tumor suppressor gene. p27 deficient mice develop pituitary tumors and display increased body...
Prostate stem cell antigen: A cell surface marker overexpressed in prostate cancer
Reiter, Robert E., Gu, Zhennen, Watabe, Tetsuro, Thomas, George, Szigeti, Kinga, Davis, Elizabeth, ...
The identification of cell surface antigens is critical to the development of new diagnostic and therapeutic modalities for the management of prostate cancer. Prostate stem cell antigen (PSCA) is a...
Bhattacharjee, Arindam, Richards, William G., Staunton, Jane, Li, Cheng, Monti, Stefano, Vasa, Priya, ...
We have generated a molecular taxonomy of lung carcinoma, the leading cause of cancer death in the United States and worldwide. Using oligonucleotide microarrays, we analyzed mRNA expression levels...
Multiclass cancer diagnosis using tumor gene expression signatures
Ramaswamy, Sridhar, Tamayo, Pablo, Rifkin, Ryan, Mukherjee, Sayan, Yeang, Chen-Hsiang, Angelo, Michael, ...
The optimal treatment of patients with cancer depends on establishing accurate diagnoses by using a complex combination of clinical and histopathological data. In some instances, this task is...
Nakamura, Noriaki, Ramaswamy, Shivapriya, Vazquez, Francisca, Signoretti, Sabina, Loda, Massimo, Sellers, William R.
PTEN acts as a tumor suppressor, at least in part, by antagonizing phosphoinositide 3-kinase (PI3K)/Akt signaling. Here we show that Forkhead transcription factors FKHRL1 and FKHR, substrates of the...
Obligate Roles for p16Ink4a and p19Arf-p53 in the Suppression of Murine Pancreatic Neoplasia
Bardeesy, Nabeel, Morgan, Jeffrey, Sinha, Manisha, Signoretti, Sabina, Srivastava, Shefali, Loda, Massimo, ...
Epithelial tumors of the pancreas exhibit a wide spectrum of histologies with varying propensities for metastasis and tissue invasion. The histogenic relationship among these tumor types is not well...
Oncogenic role of the ubiquitin ligase subunit Skp2 in human breast cancer
Signoretti, Sabina, Di Marcotullio, Lucia, Richardson, Andrea, Ramaswamy, Sridhar, Isaac, Beth, Rue, Montserrat, ...
Estrogen receptor (ER) expression and Her-2 amplification define specific subsets of breast tumors for which specific therapies exist. The S-phase kinase-associated protein Skp2 is required for the...
Turnover of several regulatory proteins results from targeted destruction via ubiquitination and subsequent degradation through the proteosome. The timely and irreversible degradation of critical...
Prostate intraepithelial neoplasia induced by prostate restricted Akt activation: The MPAKT model
Majumder, Pradip K., Yeh, Jen Jen, George, Daniel J., Febbo, Phillip G., Kum, Jennifer, Xue, Qi, ...
To determine whether Akt activation was sufficient for the transformation of normal prostate epithelial cells, murine prostate restricted Akt kinase activity was generated in transgenic mice (MPAKT...
Role of the Cdc25A phosphatase in human breast cancer
Cangi, M. Giulia, Cukor, Barry, Soung, Peggy, Signoretti, Sabina, Moreira, Gilberto, Ranashinge, Moksha, ...
The phosphatase Cdc25A plays an important role in cell cycle regulation by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases, and it has been shown to...
Genetic Interaction between Rb and K-ras in the Control of Differentiation and Tumor Suppression
Takahashi, Chiaki, Contreras, Bernardo, Bronson, Roderick T., Loda, Massimo, Ewen, Mark E.
Although the retinoblastoma protein (pRb) has been implicated in the processes of cellular differentiation, there is no compelling genetic or in vivo evidence that such activities contribute to...
Luo, Ji, Sobkiw, Cassandra L., Logsdon, Nicole M., Watt, John M., Signoretti, Sabina, O'Connell, Fionnuala, ...
Mice with heterozygous deletion of the PTEN tumor suppressor gene develop a range of epithelial neoplasia as well as lymphoid hyperplasia. Previous studies suggest that PTEN suppresses tumor...
p63 regulates commitment to the prostate cell lineage
Signoretti, Sabina, Pires, Maira M., Lindauer, Meghan, Horner, James W., Grisanzio, Chiara, Dhar, Sonya, ...
Molecular mechanisms underlying prostate and urothelial development remain unclear. This situation presents major limitations in identifying the cell type(s) and molecular events involved in the...
Human Kidney Injury Molecule-1 Is a Tissue and Urinary Tumor Marker of Renal Cell Carcinoma
Han, Won K., Alinani, Anwar, Wu, Chin-Lee, Michaelson, Dror, Loda, Massimo, McGovern, Francis J., ...
Human kidney injury molecule-1 (hKIM-1) is a type 1 transmembrane protein that is not detectable in normal kidney tissue but is expressed at high levels in human and rodent kidneys with...
Ogino, Shuji, Brahmandam, Mohan, Kawasaki, Takako, Kirkner, Gregory J, Loda, Massimo, Fuchs, Charles S
Cyclooxygenase-2 (COX-2) overexpression and mutations of p53 (a known COX-2 regulator) are inversely associated with microsatellite instability—high (MSI-H) and CpG island methylator phenotype...
Prostate stem cell antigen: A cell surface marker overexpressed in prostate cancer
Reiter, Robert E., Gu, Zhennen, Watabe, Tetsuro, Thomas, George, Szigeti, Kinga, Davis, Elizabeth, ...
The identification of cell surface antigens is critical to the development of new diagnostic and therapeutic modalities for the management of prostate cancer. Prostate stem cell antigen (PSCA) is a...
Bhattacharjee, Arindam, Richards, William G., Staunton, Jane, Li, Cheng, Monti, Stefano, Vasa, Priya, ...
We have generated a molecular taxonomy of lung carcinoma, the leading cause of cancer death in the United States and worldwide. Using oligonucleotide microarrays, we analyzed mRNA expression levels...
Multiclass cancer diagnosis using tumor gene expression signatures
Ramaswamy, Sridhar, Tamayo, Pablo, Rifkin, Ryan, Mukherjee, Sayan, Yeang, Chen-Hsiang, Angelo, Michael, ...
The optimal treatment of patients with cancer depends on establishing accurate diagnoses by using a complex combination of clinical and histopathological data. In some instances, this task is...
Nakamura, Noriaki, Ramaswamy, Shivapriya, Vazquez, Francisca, Signoretti, Sabina, Loda, Massimo, Sellers, William R.
PTEN acts as a tumor suppressor, at least in part, by antagonizing phosphoinositide 3-kinase (PI3K)/Akt signaling. Here we show that Forkhead transcription factors FKHRL1 and FKHR, substrates of the...
Obligate Roles for p16Ink4a and p19Arf-p53 in the Suppression of Murine Pancreatic Neoplasia
Bardeesy, Nabeel, Morgan, Jeffrey, Sinha, Manisha, Signoretti, Sabina, Srivastava, Shefali, Loda, Massimo, ...
Epithelial tumors of the pancreas exhibit a wide spectrum of histologies with varying propensities for metastasis and tissue invasion. The histogenic relationship among these tumor types is not well...
Oncogenic role of the ubiquitin ligase subunit Skp2 in human breast cancer
Signoretti, Sabina, Di Marcotullio, Lucia, Richardson, Andrea, Ramaswamy, Sridhar, Isaac, Beth, Rue, Montserrat, ...
Estrogen receptor (ER) expression and Her-2 amplification define specific subsets of breast tumors for which specific therapies exist. The S-phase kinase-associated protein Skp2 is required for the...
Turnover of several regulatory proteins results from targeted destruction via ubiquitination and subsequent degradation through the proteosome. The timely and irreversible degradation of critical...
Prostate intraepithelial neoplasia induced by prostate restricted Akt activation: The MPAKT model
Majumder, Pradip K., Yeh, Jen Jen, George, Daniel J., Febbo, Phillip G., Kum, Jennifer, Xue, Qi, ...
To determine whether Akt activation was sufficient for the transformation of normal prostate epithelial cells, murine prostate restricted Akt kinase activity was generated in transgenic mice (MPAKT...
Role of the Cdc25A phosphatase in human breast cancer
Cangi, M. Giulia, Cukor, Barry, Soung, Peggy, Signoretti, Sabina, Moreira, Gilberto, Ranashinge, Moksha, ...
The phosphatase Cdc25A plays an important role in cell cycle regulation by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases, and it has been shown to...
Genetic Interaction between Rb and K-ras in the Control of Differentiation and Tumor Suppression
Takahashi, Chiaki, Contreras, Bernardo, Bronson, Roderick T., Loda, Massimo, Ewen, Mark E.
Although the retinoblastoma protein (pRb) has been implicated in the processes of cellular differentiation, there is no compelling genetic or in vivo evidence that such activities contribute to...
Luo, Ji, Sobkiw, Cassandra L., Logsdon, Nicole M., Watt, John M., Signoretti, Sabina, O'Connell, Fionnuala, ...
Mice with heterozygous deletion of the PTEN tumor suppressor gene develop a range of epithelial neoplasia as well as lymphoid hyperplasia. Previous studies suggest that PTEN suppresses tumor...
p63 regulates commitment to the prostate cell lineage
Signoretti, Sabina, Pires, Maira M., Lindauer, Meghan, Horner, James W., Grisanzio, Chiara, Dhar, Sonya, ...
Molecular mechanisms underlying prostate and urothelial development remain unclear. This situation presents major limitations in identifying the cell type(s) and molecular events involved in the...
Ogino, Shuji, Brahmandam, Mohan, Kawasaki, Takako, Kirkner, Gregory J, Loda, Massimo, Fuchs, Charles S
Cyclooxygenase-2 (COX-2) overexpression and mutations of p53 (a known COX-2 regulator) are inversely associated with microsatellite instability—high (MSI-H) and CpG island methylator phenotype...
Down-Regulation of p27 Is Associated with Development of Colorectal Adenocarcinoma Metastases
Thomas, George V., Szigeti, Kinga, Murphy, Michael, Draetta, Giulio, Pagano, Michele, Loda, Massimo
The cyclin-dependent kinase inhibitor p27 is a negative regulator of the cell cycle and a potential tumor suppressor gene. Because we had previously demonstrated that loss of p27 protein is...
Signoretti, Sabina, Murphy, Michael, Cangi, Maria Giulia, Puddu, Pietro, Kadin, Marshall E., Loda, Massimo
The diagnosis of T-cell lymphoproliferative disorders, which frequently involve the skin and other extranodal sites, is often problematic because of the difficulty in establishing clonality in...
Leav, Irwin, Merk, Frederick B., Lee, Kai Fai, Loda, Massimo, Mandoki, Mira, McNeal, John E., ...
In situ hybridization and immunohistochemistry were used to localize and compare the expression of the long form of the human prolactin receptor in fetal, prepubertal, and adult prostate. Results...
Walsh, Shaun, Murphy, Michael, Silverman, Mark, Odze, Robert, Antonioli, Donald, Goldman, Harvey, ...
The cyclin-dependent kinase inhibitor p27 is a negative regulator of the transition from G1 to S phase of the cell cycle, protects against inflammatory injury and promotes epithelial differentiation....
p63 Is a Prostate Basal Cell Marker and Is Required for Prostate Development
Signoretti, Sabina, Waltregny, David, Dilks, James, Isaac, Beth, Lin, Douglas, Garraway, Levi, ...
The p53 homologue p63 encodes for different isotypes able to either transactivate p53 reporter genes (TAp63) or act as p53-dominant-negatives (ΔNp63). p63 is expressed in the basal cells of many...
Sensitive Sequencing Method for KRAS Mutation Detection by Pyrosequencing
Ogino, Shuji, Kawasaki, Takako, Brahmandam, Mohan, Yan, Liying, Cantor, Mami, Namgyal, Chungdak, ...
Both benign and malignant tumors represent heterogenous tissue containing tumor cells and non-neoplastic mesenchymal and inflammatory cells. To detect a minority of mutant KRAS alleles among abundant...
Ogino, Shuji, Kawasaki, Takako, Brahmandam, Mohan, Cantor, Mami, Kirkner, Gregory J., Spiegelman, Donna, ...
Assays to measure DNA methylation, which are important in epigenetic research and clinical diagnostics, typically rely on conversion of unmethylated cytosine to uracil by sodium bisulfite. However,...
Ogino, Shuji, Kawasaki, Takako, Kirkner, Gregory J., Loda, Massimo, Fuchs, Charles S.
The CpG island methylator phenotype (CIMP or CIMP-high) with extensive promoter methylation seems to be a distinct epigenotype of colorectal cancer. However, no study has comprehensively examined...
Febbo, Phillip G, Mulligan, Mike G, Slonina, David A, Stegmeir, Kimberly, Di Vizio, Dolores, Martinez, Paul R, ...
Semiautomated Multiplexed Quantum Dot-Based in Situ Hybridization and Spectral Deconvolution
Byers, Richard J., Di Vizio, Dolores, O’Connell, Fionnuala, Tholouli, Eleni, Levenson, Richard M., Gossard, Kirk, ...
Gene expression profiling has identified several potentially useful gene signatures for predicting outcome or for selecting targeted therapy. However, these signatures have been developed in fresh or...
Identification of prostate cancer modifier pathways using parental strain expression mapping
Xu, Qing, Majumder, Pradip K., Ross, Kenneth, Shim, Yeonju, Golub, Todd R., Loda, Massimo, ...
Inherited genetic risk factors play an important role in cancer. However, other than the Mendelian fashion cancer susceptibility genes found in familial cancer syndromes, little is known about risk...
Potential role of miR-9 and miR-223 in recurrent ovarian cancer
Laios, Alexandros, O'Toole, Sharon, Flavin, Richard, Martin, Cara, Kelly, Lynn, Ring, Martina, ...
Ogino, Shuji, Kawasaki, Takako, Kirkner, Gregory J., Kraft, Peter, Loda, Massimo, Fuchs, Charles S.
The CpG island methylator phenotype (CIMP or CIMP-high) with extensive promoter methylation is a distinct phenotype in colorectal cancer. However, a choice of markers for CIMP has been controversial....
DNA Degradation Test Predicts Success in Whole-Genome Amplification from Diverse Clinical Samples
Wang, Fengfei, Wang, Lilin, Briggs, Christine, Sicinska, Ewa, Gaston, Sandra M., Mamon, Harvey, ...
The need to apply modern technologies to analyze DNA from diverse clinical samples often stumbles on suboptimal sample quality. We developed a simple approach to assess DNA fragmentation in minute...
Nanni, Simona, Benvenuti, Valentina, Grasselli, Annalisa, Priolo, Carmen, Aiello, Aurora, Mattiussi, Stefania, ...
The identification of biomarkers that distinguish between aggressive and indolent forms of prostate cancer (PCa) is crucial for diagnosis and treatment. In this study, we used cultured cells derived...
p63 Promotes Cell Survival through Fatty Acid Synthase
Sabbisetti, Venkata, Di Napoli, Arianna, Seeley, Apryle, Amato, Angela M., O'Regan, Esther, Ghebremichael, Musie, ...
Systems-Level Modeling of Cancer-Fibroblast Interaction
Wadlow, Raymond C., Wittner, Ben S., Finley, S. Aidan, Bergquist, Henry, Upadhyay, Rabi, Finn, Stephen, ...
Cancer cells interact with surrounding stromal fibroblasts during tumorigenesis, but the complex molecular rules that govern these interactions remain poorly understood thus hindering the development...