Pearson, Todd, Shultz, Leonard D., Lief, J., Burzenski, Lisa M., Gott, Bruce, Chase, T., ...
AIMS/HYPOTHESIS: To develop and validate a new immunodeficient mouse strain that spontaneously develops a non-autoimmune hyperglycaemia to serve as a diabetic host for human islets and human beta...
King, Marie A., Pearson, Todd, Shultz, Leonard D., Leif, Jean H., Bottino, Rita, Trucco, Massimo, ...
Immunodeficient NOD-scid mice bearing a targeted mutation in the IL2 receptor common gamma chain (Il2rgamma(null)) readily engraft with human stem cells. Here we analyzed human peripheral blood...
Development of new-generation HU-PBMC-NOD/SCID mice to study human islet alloreactivity (2007)
King, Marie A., Pearson, Todd, Shultz, Leonard D., Leif, Jean H., Bottino, Rita, Trucco, Massimo, ...
The use of "humanized" mice represents an appealing translational model for studies of the pathogenesis of immune-mediated diseases and for the evaluation of potential therapeutics. The utility of...
Development of new-generation HU-PBMC-NOD/SCID mice to study human islet alloreactivity (2007)
King, Marie A., Pearson, Todd, Shultz, Leonard D., Leif, Jean, Bottino, Rita, Trucco, Massimo, ...
The use of "humanized" mice represents an appealing translational model for studies of the pathogenesis of immune-mediated diseases and for the evaluation of potential therapeutics. The utility of...
Corcoran, David L., Feingold, Eleanor, Dominick, Jessica, Wright, Marietta, Harnaha, Jo, Trucco, Massimo, ...
The search for mammalian DNA regulatory regions poses a challenging problem in computational biology. The short length of the DNA patterns compared with the size of the promoter regions and the...
Ringquist, Steven, Pecoraro, Christopher, Gilchrist, Crystal M. S., Styche, Alexis, Rudert, William A., Benos, Panagiotis V., ...
SOP3v2 is a database-driven graphical web-based application for facilitating genotyping assay design. SOP3v2 accepts data input in numerous forms, including gene names, reference sequence numbers and...
Gene Combination Transfer to Block Autoimmune Damage in Transplanted Islets of Langerhans (2004)
Suzanne Bertera, Angela M. Alexander, Megan L. Crawford, Glenn Papworth, Simon C. Watkins, Paul D. Robbins, ...
Islet transplantation therapy would be applicable to a wider range of diabetic patients if donor islet acceptance and protection were possible without systemic immunosuppression of the recipient. To...
Regulation of FasL/Fas in Human Trophoblasts: Possible Implications for Chorioamnionitis1 (2003)
Dhruv R Balkundi, Judy A Ziegler, Jon F Watchko, Catherine Craven, Massimo Trucco
Chorioamnionitis is a common cause of premature birth and is associated with significant morbidity and mortality in the mother and infant. Preterm birth shares similarities with rejection of the...
New Advanced Technology to Improve Prediction and Prevention of Type 1 Diabetes (2003)
Type I diabetes is considered an autoimmune disease characterized by the presence of lymphocytes in the islets of Langerhans. The majority of infiltrating cells are actually T lymphocytes that are...
The activities of the Children's Hospital of Pittsburgh Histocompatibility Center can be classified under three major categories: 1) the repository service; 2) the molecular typing service; and 3)...
The activities of the Children's Hospital of Pittsburgh Histocompatibility Center can be classified under three major categories: 1) the repository service; 2) the molecular typing service; and 3)...
The activities of the Children's Hospital of Pittsburgh Histocompatibility Center can be classified under three major categories: (1) the repository service; (2) the molecular typing service; and (3)...
High frequencies of T-cell receptor (TCR) VBeta7+ T-cells are detected among lymphocytes isolated from pancreatic islets of children at the onset of Type 1 diabetes. Peripheral blood mononuclear...
New Advanced Technology to Improve Prediction and Prevention of Type I Diabetes (1998)
Type 1 diabetes is considered an autoimmune disease characterized by the presence of lymphocytes in the islets of Langerhans. The majority of infiltrating cells are actually T lymphocytes that are...
New Advanced Technology to Improve Prediction and Prevention of Type 1 Diabetes (1998)
Type 1 diabetes is considered an auto immune disease characterized by the presence of inflammatory cells in the islets of Langerhas. These cells are T lymphocytes, considered responsible for the...
New Advanced Technology to Improve Prediction & Prevention of Type 1 Diabetes (1998)
The research hypothesis on which our project is based is that there is a genetic contribution associated with increased risk for developing diabetic complications, specifically diabetic-nephropathy....
Chicz, Roman M., Lane, William S., Robinson, Renée A., Trucco, Massimo, Strominger, Jack L., Gorga, Joan C.
Genetic susceptibility to several autoimmune disorders is associated with the expression of certain MHC class II alleles. Insight into the etiology of such diseases awaits the identification of the...
Dinucleotide repeat polymorphisms at the D13S192 and D13S193 loci (1993)
Hong, Hee-Kyung, Giorda, Roberto, Trucco, Massimo, Chakravarti, Aravinda
Microsatellite repeat polymorphism at the D13S197 locus (1993)
Hong, Hee-Kyung, Giorda, Roberto, Yu, Ling Mei, Trucco, Massimo, Chakravarti, Aravinda
Dinucleotide repeat polymorphism at the DXS1146 locus (1993)
Hong, Hee-Kyung, Giorda, Roberto, Trucco, Massimo, Chakravarti, Aravinda
B.Lipton, Rebecca, Kocova, Mirjana, LaPorte, Ronald E., Dorman, Janice S., Orchard, Trevor J., Riley, William J., ...
The risk for insulin-dependent diabetes mellitus (IDDM) associated with genetic susceptibility markers at the human leukocyte antigen (HLA) DQA1 and DQB1 loci was evaluated among individuals with and...
Böhm, Bernhard Otto, Seißler, Jochen, Glück, Michael, Manfras, Burkhard J., Thomas, H., Schmidt, K., ...
Böhm, Bernhard Otto, Manfras, Burkhard J., Seißler, Jochen, Schöffling, Karl, Glück, Michael, Holzberger, G., ...
Islet cell antibodies (ICAs) were determined in a large cohort of white nondiabetic schoolchildren (n = 4287) from a homogenous population in southern Germany. The prevalence of ICA levels greater...
TaqMan-Based Detection of Trichomonas vaginalis DNA from Female Genital Specimens
Jordan, Jeanne A., Lowery, Donna, Trucco, Massimo
A double-labeled fluorescent probe was designed and evaluated for detecting Trichomonas vaginalis DNA in a 5′ nuclease (TaqMan) assay. The T. vaginalis-specific probe contains a 5′-fluorescein...
Naturally processed and presented epitopes of the islet cell autoantigen IA-2 eluted from HLA-DR4
Peakman, Mark, Stevens, Elizabeth J., Lohmann, Tobias, Narendran, Parth, Dromey, James, Alexander, Angela, ...
During immune responses, antigen-presenting cells (APCs) process antigens and present peptide epitopes complexed with human leukocyte antigen (HLA) molecules. CD4 cells recognize these naturally...
Regeneration of the pancreatic β cell
Type 1 diabetes is the result of an autoimmune attack against the insulin-producing β cells of the endocrine pancreas. Current treatment for patients with type 1 diabetes typically involves a...
Corcoran, David L., Feingold, Eleanor, Dominick, Jessica, Wright, Marietta, Harnaha, Jo, Trucco, Massimo, ...
The search for mammalian DNA regulatory regions poses a challenging problem in computational biology. The short length of the DNA patterns compared with the size of the promoter regions and the...
Ringquist, Steven, Pecoraro, Christopher, Gilchrist, Crystal M. S., Styche, Alexis, Rudert, William A., Benos, Panagiotis V., ...
SOP3v2 is a database-driven graphical web-based application for facilitating genotyping assay design. SOP3v2 accepts data input in numerous forms, including gene names, reference sequence numbers and...
TaqMan-Based Detection of Trichomonas vaginalis DNA from Female Genital Specimens
Jordan, Jeanne A., Lowery, Donna, Trucco, Massimo
A double-labeled fluorescent probe was designed and evaluated for detecting Trichomonas vaginalis DNA in a 5′ nuclease (TaqMan) assay. The T. vaginalis-specific probe contains a 5′-fluorescein...
Naturally processed and presented epitopes of the islet cell autoantigen IA-2 eluted from HLA-DR4
Peakman, Mark, Stevens, Elizabeth J., Lohmann, Tobias, Narendran, Parth, Dromey, James, Alexander, Angela, ...
During immune responses, antigen-presenting cells (APCs) process antigens and present peptide epitopes complexed with human leukocyte antigen (HLA) molecules. CD4 cells recognize these naturally...
Regeneration of the pancreatic β cell
Type 1 diabetes is the result of an autoimmune attack against the insulin-producing β cells of the endocrine pancreas. Current treatment for patients with type 1 diabetes typically involves a...
Corcoran, David L., Feingold, Eleanor, Dominick, Jessica, Wright, Marietta, Harnaha, Jo, Trucco, Massimo, ...
The search for mammalian DNA regulatory regions poses a challenging problem in computational biology. The short length of the DNA patterns compared with the size of the promoter regions and the...
Ringquist, Steven, Pecoraro, Christopher, Gilchrist, Crystal M. S., Styche, Alexis, Rudert, William A., Benos, Panagiotis V., ...
SOP3v2 is a database-driven graphical web-based application for facilitating genotyping assay design. SOP3v2 accepts data input in numerous forms, including gene names, reference sequence numbers and...
Functionally important glycosyltransferase gain and loss during catarrhine primate emergence
Koike, Chihiro, Uddin, Monica, Wildman, Derek E., Gray, Edward A., Trucco, Massimo, Starzl, Thomas E., ...
A glycosyltransferase, α1,3galactosyltransferase, catalyzes the terminal step in biosynthesis of Galα1,3Galβ1–4GlcNAc-R (αGal), an oligosaccharide cell surface epitope. This epitope or...
Stassi, Giorgio, Maria, Ruggero De, Trucco, Giuliana, Rudert, William, Testi, Roberto, Galluzzo, Aldo, ...
Fas is an apoptosis-inducing surface receptor involved in controlling tissue homeostasis and function at multiple sites. Here we show that β cells from the pancreata of newly diagnosed...
Increased Expression of Monocyte CD11b (Mac-1) in Overweight Recent-Onset Type 1 Diabetic Children
Cifarelli, Vincenza, Libman, Ingrid M., DeLuca, Angela, Becker, Dorothy, Trucco, Massimo, Luppi, Patrizia
AIM: Compelling evidence implicates inflammation in the pathogenesis of type 1 diabetes mellitus (T1DM) and associated vascular complications. Obesity is also characterized by low-grade systemic...
Gene Combination Transfer to Block Autoimmune Damage in Transplanted Islets of Langerhans
Bertera, Suzanne, Alexander, Angela M., Crawford, Megan L., Papworth, Glenn, Watkins, Simon C., Robbins, Paul D., ...
Islet transplantation therapy would be applicable to a wider range of diabetic patients if donor islet acceptance and protection were possible without systemic immunosuppression of the recipient. To...
The Role of External and Matrix pH in Mitochondrial Reactive Oxygen Species Generation*
Selivanov, Vitaly A., Zeak, Jennifer A., Roca, Josep, Cascante, Marta, Trucco, Massimo, Votyakova, Tatyana V.
Reactive oxygen species (ROS) generation in mitochondria as a side product of electron and proton transport through the inner membrane is important for normal cell operation as well as development of...
Luca, Diana, Ringquist, Steven, Klei, Lambertus, Lee, Ann B., Gieger, Christian, Wichmann, H.-Erich, ...
Resources being amassed for genome-wide association (GWA) studies include “control databases” genotyped with a large-scale SNP array. How to use these databases effectively is an open question....