Mathias Sprinzl

tRNAdb 2009: compilation of tRNA sequences and tRNA genes (2009)

Jühling, Frank, Mörl, Mario, Hartmann, Roland K., Sprinzl, Mathias, Stadler, Peter F., Pütz, Joern

One of the first specialized collections of nucleic acid sequences in life sciences was the ‘compilation of tRNA sequences and sequences of tRNA genes’ (http://www.trna.uni-bayreuth.de). Here, an...

Release Factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies (2007)

Zoldák, Gabriel, Redecke, Lars, Svergun, Dmitri I., Konarev, Peter V., Voertler, C. Stefan, Dobbek, Holger, ...

Prokaryotic class I release factors (RFs) respond to mRNA stop codons and terminate protein synthesis. They interact with the ribosomal decoding site and the peptidyl-transferase centre bridging...

Release Factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies (2007)

Zoldák, Gabriel, Redecke, Lars, Svergun, Dmitri I., Konarev, Peter V., Voertler, C. Stefan, Dobbek, Holger, ...

Prokaryotic class I release factors (RFs) respond to mRNA stop codons and terminate protein synthesis. They interact with the ribosomal decoding site and the peptidyl-transferase centre bridging...

Compilation of tRNA sequences and sequences of tRNA genes (2005)

Sprinzl, Mathias, Vassilenko, Konstantin S.

Maintained at the Universität Bayreuth, Bayreuth, Germany, the Compilation of tRNA Sequences and Sequences of tRNA Genes is accessible at the URL http://www.tRNA.uni-bayreuth.de with mirror site...

Compilation of tRNA sequences and sequences of tRNA genes (1998)

Mathias Sprinzl, Clemens Steegborn, Frank Hübel, Sergey Steinberg

tRNA genes. The last edition which appeared two years ago (1) was supplemented by 694 new sequences. The sequences of tRNA mutants and of tRNAs originating from transformed or

Stability of triple helices containing RNA and DNA strands: experimental and molecular modeling studies (1993)

Escudeé, Christophe, Francçois, Jean-Chritophe, Sun, Jian-Sheng, Ott, Guünther, Sprinzl, Mathias, Garestier, Theére`se, ...

UV-absorption spectrophotometry and molecular modeling have been used to study the influence of the chemical nature of sugars (ribose or deoxyribose) on triple helix stability. For the...

Structure of the phenylalnyl-tRNA synthetase genes from Thermus thermophilus HB8 and their expression in Escherichia coli (1992)

Kreutzer, Roland, Kruft, Volker, Bobkova, Ekaterina V., Lavrik, Olga I., Sprinzl, Mathias

A 4459 bp long BamHI restriction fragment containing the two genes for the Thermus thermophilus HB8 phenylalanyt-tRNA synthetase was cloned in Escherichia coll and its nucleotlde sequence was...

Interaction of the isolated domain II/III of Thermus thermophilus elongation factor Tu with the nucleotide exchange factor EF-Ts (1990)

Peter, Marcus E., Reiser, Christian O.A., Schirmer, Norbert K., Kiefhaber, Thomas, Ott, Günther, Grillenbeck, Norbert W., ...

The middle and C-terminal domain (domain II/III) of elongation factor Tu from Thermus thermophilus lacking the GTP/GDP binding domain have been prepared by treating nucleotide-free protein with...

The role of modified purine 64 in initiator/elongator discrimination of tRNAiMet from yeast and wheat germ (1990)

Kiesewetter, Stefan, Ott, Günther, Sprinzl, Mathias

The role of 2′-ribosylated adenosine 64 in tRNAiMet from yeast in initiation/elongation discrimination was investigated. As measured by in vitro translation in rabbit reticulocyte lysate, the...

The influence of different modifications of elongation factor Tu from Escherichia coli on ternary complex formation investigated by fluorescence spectroscopy (1990)

Ott, Günther, Jonák, Jiri, Abrahams, Ian Peter, Sprinzl, Mathias

A fluorescence titration assay was used to detect the effects of various modifications of E.coli elongation factor Tu on the formation of the ternary complex with aminoacyl-tRNAs. The treatment of...

Interaction of a selenocysteine-incorporating tRNA with elongation factor Tu from E.coli (1990)

Förster, Charlotte, Ott, Günther, Forchhammer, Karl, Sprinzl, Mathias

Selenocysteine-incorporating tRNASec(UCA), the product of selC, was isolated from E.coli and aminoacylated with serine. The equilibrium dissociation constant for the interaction of Ser- tRNASec(UCA)...

Sequence and identification of the nucleotide binding site for the elongation factor Tu from Thermus thermophilus HB8 (1987)

Seidler, Lothar, Peter, Marcus, Meissner, Franz, Sprinzl, Mathias

Two structural genes for the Thermus thermophilus elongation factor Tu (tuf) were identified by cross-hybridization with the tufA gene from E.coli. The sequence of one of these tuf genes, localized...

Ser-tRNAs from bovine mitochondrion form ternary complexes with bacterial elongation factor Tu and GTP (1986)

Gebhardt-Singh, Eva, Sprinzl, Mathias

Transfer ribonucleic acids were isolated from mitochondria of bovine heart and aminoacylated in vitro by a crude mitochondrial enzyme. Ser-$${\hbox{ tRNA }}_{\hbox{ UCN }}^{\hbox{ Ser }}$$ and...

Interaction of unfolded tRNA with the 3'-terminal region of E. coli 16S ribosomal RNA (1985)

Helk, Bernhard, Sprinzl, Mathias

Fragments of tRNA possessing a free TψC-loop or a free D-loop form stable complexes with the colicin fragment (1494–1542) of 16S ribosomal RNA from E. coli. The colicin fragment does not bind to...

Chemical synthesis of 5-azacytidlne nucleotides and preparation of tRNAs containing 5-azacytidine in its 3'-terminus (1984)

Zidinski, Wojciech S., Sprinzl, Mathias

5-azacytidine-5′-triphosphate prepared from 5-azacytidine by chemical phosphorylation is a substrate for AMP(CMP)tRNA nucleo-tidyl transferase from yeast. tRNAsphe from yeast containing...

Aminoacyl RNA domain of turnip yellow mosaic virus Val-RNA interacting with elongation factor Tu (1984)

Joshi, Rajiv L., Faulhammer, Heinz, Chapeville, François, Sprinzl, Mathias, Haenni, Anne-Lise

Turnip yellow mosaic virus(TYMV) Val-RNA forms a complex with the pep-tide elongation factor Tu (EF-Tu) in the presence of GTP: the Val-RNA is protected by EF-Tu.GTP from non-enzymatic deacylation...

Role of the 5'-terminal phosphate of tRNA for its function during protein biosynthesis elongation cycle (1980)

Sprinzl, Mathias, Graeser, Erika

The 5′-terminal phosphate of tRNAPhe from yeast was removed using tRNAPhe lacking its 3′-terminal adenosine. After regeneration of the C-C-A terminus this tRNA was investigated in following...

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance (1979)

Davanloo, Pari, Sprinzl, Mathias, Watanabe, Kimitsuna, Albani, Martin, Kersten, Helga

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E.coli, from a mutant thereof, and from...

Properties of tRNAPhe from yeast carrying a spin label on the 3'-terminal. Interaction with yeast phenylalanyl-tRNA synthetase and elongation factor Tu from Escherichia coli (1978)

Sprinzl, Mathias, Siboska, Gunhild E., Pedersen, Jens A.

The 2-thioketo function of tRNA Phe-C-S2C-A in which the penultimate cytidine residue is replaced by 2-thiocytidine can serve as site of specific attachment of spin label. By alkylatio...

Participation of X47-fluorescamine modified E. coli tRNAs in in vitro protein biosynthesis (1978)

Sprinzl, Mathias, Faulhammer, Heinz G.

The reaction of fluorescamine with primary amino groups of tRNAs was investigated. The reagent was attached under mild conditions to the 3′-end of tRNAPhe-C-C-A(3′NH2) from yeast and to the minor...

Versuche zur Synthese des Antibiotikums Formycin / (1971)

Sprinzl, Mathias.

Thesis (doctoral)--Technische Universität Carolo-Wilhelmina zu Braunschweig, 1971.

Aminoacyl RNA domain of turnip yellow mosaic virus Val-RNA interacting with elongation factor Tu

Joshi, Rajiv L., Faulhammer, Heinz, Chapeville, François, Sprinzl, Mathias, Haenni, Anne-Lise

Turnip yellow mosaic virus (TYMV) Val-RNA forms a complex with the peptide elongation factor Tu (EF-Tu) in the presence of GTP: the Val-RNA is protected by EF-Tu·GTP from non-enzymatic deacylation...

A New Transfer RNA Fragment Reaction: TpΨpCpGp Bound to a Ribosome-Messenger RNA Complex Induces the Synthesis of Guanosine Tetra- and Pentaphosphates

Richter, Dietmar, Erdmann, Volker A., Sprinzl, Mathias

The tetranucleotide TpΨpCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The...

Compilation of tRNA sequences and sequences of tRNA genes

Sprinzl, Mathias, Vassilenko, Konstantin S.

Maintained at the Universität Bayreuth, Bayreuth, Germany, the Compilation of tRNA Sequences and Sequences of tRNA Genes is accessible at the URL http://www.tRNA.uni-bayreuth.de with mirror site...

Aminoacyl RNA domain of turnip yellow mosaic virus Val-RNA interacting with elongation factor Tu

Joshi, Rajiv L., Faulhammer, Heinz, Chapeville, François, Sprinzl, Mathias, Haenni, Anne-Lise

Turnip yellow mosaic virus (TYMV) Val-RNA forms a complex with the peptide elongation factor Tu (EF-Tu) in the presence of GTP: the Val-RNA is protected by EF-Tu·GTP from non-enzymatic deacylation...

A New Transfer RNA Fragment Reaction: TpΨpCpGp Bound to a Ribosome-Messenger RNA Complex Induces the Synthesis of Guanosine Tetra- and Pentaphosphates

Richter, Dietmar, Erdmann, Volker A., Sprinzl, Mathias

The tetranucleotide TpΨpCpGp, when bound to Escherichia coli 50S ribosomal subunits, can replace intact tRNA in the stringent factor-directed synthesis of guanosine tetra- and pentaphosphates. The...

Compilation of tRNA sequences and sequences of tRNA genes

Sprinzl, Mathias, Vassilenko, Konstantin S.

Maintained at the Universität Bayreuth, Bayreuth, Germany, the Compilation of tRNA Sequences and Sequences of tRNA Genes is accessible at the URL http://www.tRNA.uni-bayreuth.de with mirror site...

Release Factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies

Zoldák, Gabriel, Redecke, Lars, Svergun, Dmitri I., Konarev, Peter V., Voertler, C. Stefan, Dobbek, Holger, ...

Prokaryotic class I release factors (RFs) respond to mRNA stop codons and terminate protein synthesis. They interact with the ribosomal decoding site and the peptidyl-transferase centre bridging...

tRNAdb 2009: compilation of tRNA sequences and tRNA genes

Jühling, Frank, Mörl, Mario, Hartmann, Roland K., Sprinzl, Mathias, Stadler, Peter F., Pütz, Joern

One of the first specialized collections of nucleic acid sequences in life sciences was the ‘compilation of tRNA sequences and sequences of tRNA genes’ (http://www.trna.uni-bayreuth.de). Here, an...