Matthew D. Welch

Repetitive N-WASP-binding elements of the enterohemorrhagic Escherichia coli effector EspF(U) synergistically activate actin assembly (2008)

Campellone, Kenneth Geno, Cheng, Hui-Chun, Robbins, Douglas, Siripala, Anosha D., McGhie, Emma J., Hayward, Richard D., ...

Enterohemorrhagic Escherichia coli (EHEC) generate F-actin-rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along...

WHAMM is an Arp2/3 complex activator that binds microtubules and functions in ER to Golgi transport (2008)

Campellone, Kenneth Geno, Webb, Neil J., Znameroski, Elizabeth A., Welch, Matthew D.

The Arp2/3 complex is an actin nucleator that plays a critical role in many cellular processes. Its activities are regulated by nucleation-promoting factors (NPFs) that function primarily during...

Motility Determinants in WASP Family ProteinsD⃞

Yarar, Defne, D'Alessio, Joseph A., Jeng, Robert L., Welch, Matthew D.

In response to upstream signals, proteins in the Wiskott-Aldrich Syndrome protein (WASP) family regulate actin nucleation via the Arp2/3 complex. Despite intensive study of the function of WASP...

Plasma Membrane Organization Is Essential for Balancing Competing Pseudopod- and Uropod-promoting Signals during Neutrophil Polarization and MigrationD⃞V⃞

Bodin, Stéphane, Welch, Matthew D.

Exposure of neutrophils to chemoattractant induces cell polarization and migration. These behaviors require the asymmetric activation of distinct signaling pathways and cytoskeletal elements in the...

Motility Determinants in WASP Family ProteinsD⃞

Yarar, Defne, D'Alessio, Joseph A., Jeng, Robert L., Welch, Matthew D.

In response to upstream signals, proteins in the Wiskott-Aldrich Syndrome protein (WASP) family regulate actin nucleation via the Arp2/3 complex. Despite intensive study of the function of WASP...

Plasma Membrane Organization Is Essential for Balancing Competing Pseudopod- and Uropod-promoting Signals during Neutrophil Polarization and MigrationD⃞V⃞

Bodin, Stéphane, Welch, Matthew D.

Exposure of neutrophils to chemoattractant induces cell polarization and migration. These behaviors require the asymmetric activation of distinct signaling pathways and cytoskeletal elements in the...

Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility

Stamm, Luisa M., Morisaki, J. Hiroshi, Gao, Lian-Yong, Jeng, Robert L., McDonald, Kent L., Roth, Robyn, ...

Mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection....

The Human Arp2/3 Complex Is Composed of Evolutionarily Conserved Subunits and Is Localized to Cellular Regions of Dynamic Actin Filament Assembly

Welch, Matthew D., DePace, Angela H., Verma, Suzie, Iwamatsu, Akihiro, Mitchison, Timothy J.

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells. The human complex consists of seven subunits which include the actin related proteins Arp2 and Arp3,...

Pivotal role of VASP in Arp2/3 complex–mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility

Skoble, Justin, Auerbuch, Victoria, Goley, Erin D., Welch, Matthew D., Portnoy, Daniel A.

The Listeria monocytogenes ActA protein mediates actin-based motility by recruiting and stimulating the Arp2/3 complex. In vitro, the actin monomer-binding region of ActA is critical for stimulating...

Formation of filopodia-like bundles in vitro from a dendritic network

Vignjevic, Danijela, Yarar, Defne, Welch, Matthew D., Peloquin, John, Svitkina, Tatyana, Borisy, Gary G.

We report the development and characterization of an in vitro system for the formation of filopodia-like bundles. Beads coated with actin-related protein 2/3 (Arp2/3)–activating proteins can induce...

Three Regions within Acta Promote Arp2/3 Complex-Mediated Actin Nucleation and Listeria monocytogenes Motility

Skoble, Justin, Portnoy, Daniel A., Welch, Matthew D.

The Listeria monocytogenes ActA protein induces actin-based motility by enhancing the actin nucleating activity of the host Arp2/3 complex. Using systematic truncation analysis, we identified a...

Visualization and Molecular Analysis of Actin Assembly in Living Cells

Schafer, Dorothy A., Welch, Matthew D., Machesky, Laura M., Bridgman, Paul C., Meyer, Shelley M., Cooper, John A.

Actin filament assembly is critical for eukaryotic cell motility. Arp2/3 complex and capping protein (CP) regulate actin assembly in vitro. To understand how these proteins regulate the dynamics of...

Repetitive N-WASP–Binding Elements of the Enterohemorrhagic Escherichia coli Effector EspFU Synergistically Activate Actin Assembly

Campellone, Kenneth G., Cheng, Hui-Chun, Robbins, Douglas, Siripala, Anosha D., McGhie, Emma J., Hayward, Richard D., ...

Enterohemorrhagic Escherichia coli (EHEC) generate F-actin–rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along...