Maurice Hofnung

Comparison of kinetics of induction of DNA adducts and gene mutations by a nitrofuran compound, 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000), in the caecum and small intestine of Big BlueTM mice (2002)

Arrault, Xavier, Michel, Valérie, Quillardet, Philippe, Hofnung, Maurice, Touati, Eliette

In previous experiments, i.p. injection of the 5 nitronaphthofuran derivative 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000) to lacI transgenic Big Blue™ mice led to an increase in the...

Short-term infection with Helicobacter pylori and 1 week exposure to metronidazole does not enhance gastric mutation frequency in transgenic mice (2000)

Touati, Eliette, Hofnung, Maurice, Thiberge, Jean-Michel, Michel, Valérie, Labigne, Agnès, Jenks, Peter J.

The aim of this study was to determine whether exposure of Helicobacter pylori-infected mice to metronidazole resulted in the delivery of mutagenic compounds to the gastric epithelium via the...

7-Methoxy-2-nitronaphtho(2, l-b)furan (R7000)-induced mutation spectrum in the lacI gene of Escherichia coli: influence of SOS mutagenesis (1996)

Touati, Eliette, Krin, Evelyne, Quillardet, Philippe, Hofnung, Maurice

The mutagenic specificity of 7-methoxy-2-nitronaphtho(2, l-b)furan (R7000), a very potent genotoxie 2-nitrofuran, was investigated in the lacl gene of E.coli. To analyze the influence of...

Over coming the crypticity of a viral T cell Determinant by insertion into a chimeric bacterial protein (1996)

Lo-Man, Richard, Martineaue, Pierre, Manoury-Schwartz, Bénédicate, Hofnung, Maurice, Leclerc, Claude, Malissen, B.

Among the potential T cell determinants contained In a protein antigen, the T cell response only focuses on a few immunodomlnant T cell determinants, whereas cryptic epitopes remain hidden to the...

Palindromic units are part of a new bacterial interspersed mosaic element (BIME) (1991)

Gilson, Eric, Saurin, William, Perrin, David, Bachellier, Sophie, Hofnung, Maurice

Palindromic Units (PU or REP) were defined as DNA sequences of 40 nucleotides highly repeated on the genome of Escherichia coli and other Enterobacteriaceae. PU are found in clusters of up to six...

DNA polymerase I and a protein complex bind specifically to E.coli palindromic unit highly repetitive DNA: implications for bacterial chromosome organization (1990)

Gilson, Eric, Perrin, David, Hofnung, Maurice

Starting from a crude E. coli extract, two activities which specifically protect highly repetitive bacterial DNA sequences (called PU for Palindromic Unit or REP for Repetitive Extragenic Palindromic...

Recent developments in bacterial short-term tests for the detection of genotoxic agents (1986)

Hofnung, Maurice, Quillardet, Philippe

Sequence of the malK gene in E.coli K12 (1982)

Gilson, Eric, Nikaido, Hiroshi, Hofnung, Maurice

We present the sequence of gene malK which encodes a component of the system for maltose transport in E.coli K12. We also determined the position of deletion (S50) which fuses malK to the following...

Structure-Function Study of MalF Protein by Random Mutagenesis

Tapia, María Isabel, Mourez, Michaël, Hofnung, Maurice, Dassa, Elie

MalF is one of the two integral inner membrane proteins of the maltose-maltodextrin transport system. To identify functional regions in this protein, we characterized a collection of malF mutants...

IS1397 Is Active for Transposition into the Chromosome of Escherichia coli K-12 and Inserts Specifically into Palindromic Units of Bacterial Interspersed Mosaic Elements

Clément, Jean-Marie, Wilde, Caroline, Bachellier, Sophie, Lambert, Patricia, Hofnung, Maurice

We demonstrate that IS1397, a putative mobile genetic element discovered in natural isolates of Escherichia coli, is active for transposition into the chromosome of E. coli K-12 and inserts...

The C-Terminal Portion of the Tail Fiber Protein of Bacteriophage Lambda Is Responsible for Binding to LamB, Its Receptor at the Surface of Escherichia coli K-12

Wang, Jiang, Hofnung, Maurice, Charbit, Alain

Bacteriophage λ adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of λ, to maltose-binding...

Transposition of IS1397 in the Family Enterobacteriaceae and First Characterization of ISKpn1, a New Insertion Sequence Associated with Klebsiella pneumoniae Palindromic Units

Wilde, Caroline, Bachellier, Sophie, Hofnung, Maurice, Clément, Jean-Marie

IS1397 and ISKpn1 are IS3 family members which are specifically inserted into the loop of palindromic units (PUs). IS1397 is shown to transpose into PUs with sequences close or identical to the...

Extending the CD4+ T-Cell Epitope Specificity of the Th1 Immune Response to an Antigen Using a Salmonella enterica Serovar Typhimurium Delivery Vehicle

Lo-Man, Richard, Langeveld, Jan P. M., Dériaud, Edith, Jehanno, Muguette, Rojas, Marie, Clément, Jean-Marie, ...

We analyzed the CD4 T-cell immunodominance of the response to a model antigen (Ag), MalE, when delivered by an attenuated strain of Salmonella enterica serovar Typhimurium (SL3261*pMalE). Compared to...

Palindromic Unit-Independent Transposition of IS1397 in Yersinia pestis†

Wilde, Caroline, Bachellier, Sophie, Hofnung, Maurice, Carniel, Elisabeth, Clément, Jean-Marie

Palindromic units (PUs) are intergenic repeated sequences scattered over the chromosomes of Escherichia coli and several other enterobacteria. In the latter, IS1397, an E. coli insertion sequence...

malB Region in Escherichia coli K-12: Characterization of New Mutations

Hofnung, Maurice, Hatfield, Dolph, Schwartz, Maxime

Phenotypic characterization and mapping of more than 50 Mal− mutations located in the malB region lead one to divide the site for Mal−λs mutations (formerly called gene malB) in that region,...

Nonsense Mutations in the Maltose A Region of the Genetic Map of Escherichia coli

Hatfield, Dolph, Hofnung, Maurice, Schwartz, Maxime

The isolation of one amber mutation in malQ, one ochre mutation in malP, and seven amber mutations in malT is reported. A study of their phenotypic expressions in the presence of the amber suppressor...

Genetic Analysis of the Maltose A Region in Escherichia coli

Hatfield, Dolph, Hofnung, Maurice, Schwartz, Maxime

The genetic map of the maltose A locus of Escherichia coli contains at least three closely linked genes, malT, malP, and malQ. The order of these genes is established by deletion mapping. MalP and...

Presence of a Mouse Embryonic Antigen on Human Spermatozoa

Buc-Caron, Marie-Hélène, Gachelin, Gabriel, Hofnung, Maurice, Jacob, François

A cell-surface antigen common to mouse primitive teratocarcinoma cells, morulae, and spermatozoa and specified by the +t12 allele at the T-locus of the mouse has been shown by serological methods to...

Divergent Operons and the Genetic Structure of the Maltose B Region in ESCHERICHIA COLI K12

Hofnung, Maurice

Complementation and polarity suppression data are interpreted in terms of the genetic structure of the maltose B region. It is proposed that this region comprises two divergent operons. One operon...

Structure-Function Study of MalF Protein by Random Mutagenesis

Tapia, María Isabel, Mourez, Michaël, Hofnung, Maurice, Dassa, Elie

MalF is one of the two integral inner membrane proteins of the maltose-maltodextrin transport system. To identify functional regions in this protein, we characterized a collection of malF mutants...

IS1397 Is Active for Transposition into the Chromosome of Escherichia coli K-12 and Inserts Specifically into Palindromic Units of Bacterial Interspersed Mosaic Elements

Clément, Jean-Marie, Wilde, Caroline, Bachellier, Sophie, Lambert, Patricia, Hofnung, Maurice

We demonstrate that IS1397, a putative mobile genetic element discovered in natural isolates of Escherichia coli, is active for transposition into the chromosome of E. coli K-12 and inserts...

The C-Terminal Portion of the Tail Fiber Protein of Bacteriophage Lambda Is Responsible for Binding to LamB, Its Receptor at the Surface of Escherichia coli K-12

Wang, Jiang, Hofnung, Maurice, Charbit, Alain

Bacteriophage λ adsorbs to its Escherichia coli K-12 host by interacting with LamB, its cell-surface receptor. We fused C-terminal portions of J, the tail fiber protein of λ, to maltose-binding...

Transposition of IS1397 in the Family Enterobacteriaceae and First Characterization of ISKpn1, a New Insertion Sequence Associated with Klebsiella pneumoniae Palindromic Units

Wilde, Caroline, Bachellier, Sophie, Hofnung, Maurice, Clément, Jean-Marie

IS1397 and ISKpn1 are IS3 family members which are specifically inserted into the loop of palindromic units (PUs). IS1397 is shown to transpose into PUs with sequences close or identical to the...

Extending the CD4+ T-Cell Epitope Specificity of the Th1 Immune Response to an Antigen Using a Salmonella enterica Serovar Typhimurium Delivery Vehicle

Lo-Man, Richard, Langeveld, Jan P. M., Dériaud, Edith, Jehanno, Muguette, Rojas, Marie, Clément, Jean-Marie, ...

We analyzed the CD4 T-cell immunodominance of the response to a model antigen (Ag), MalE, when delivered by an attenuated strain of Salmonella enterica serovar Typhimurium (SL3261*pMalE). Compared to...

Palindromic Unit-Independent Transposition of IS1397 in Yersinia pestis†

Wilde, Caroline, Bachellier, Sophie, Hofnung, Maurice, Carniel, Elisabeth, Clément, Jean-Marie

Palindromic units (PUs) are intergenic repeated sequences scattered over the chromosomes of Escherichia coli and several other enterobacteria. In the latter, IS1397, an E. coli insertion sequence...

malB Region in Escherichia coli K-12: Characterization of New Mutations

Hofnung, Maurice, Hatfield, Dolph, Schwartz, Maxime

Phenotypic characterization and mapping of more than 50 Mal− mutations located in the malB region lead one to divide the site for Mal−λs mutations (formerly called gene malB) in that region,...

Nonsense Mutations in the Maltose A Region of the Genetic Map of Escherichia coli

Hatfield, Dolph, Hofnung, Maurice, Schwartz, Maxime

The isolation of one amber mutation in malQ, one ochre mutation in malP, and seven amber mutations in malT is reported. A study of their phenotypic expressions in the presence of the amber suppressor...

Genetic Analysis of the Maltose A Region in Escherichia coli

Hatfield, Dolph, Hofnung, Maurice, Schwartz, Maxime

The genetic map of the maltose A locus of Escherichia coli contains at least three closely linked genes, malT, malP, and malQ. The order of these genes is established by deletion mapping. MalP and...

Presence of a Mouse Embryonic Antigen on Human Spermatozoa

Buc-Caron, Marie-Hélène, Gachelin, Gabriel, Hofnung, Maurice, Jacob, François

A cell-surface antigen common to mouse primitive teratocarcinoma cells, morulae, and spermatozoa and specified by the +t12 allele at the T-locus of the mouse has been shown by serological methods to...

Divergent Operons and the Genetic Structure of the Maltose B Region in ESCHERICHIA COLI K12

Hofnung, Maurice

Complementation and polarity suppression data are interpreted in terms of the genetic structure of the maltose B region. It is proposed that this region comprises two divergent operons. One operon...