Ramesh Hariharan, Kartik Ch, Max L. Nibert, Tomas Kirchhausen, Tomas Kirchhausen
of clathrin-coated pits
Comparisons of the M1 genome segments and encoded μ2 proteins of different reovirus isolates (2004)
Yin, Peng, Keirstead, Natalie D, Broering, Teresa J, Arnold, Michelle M, Parker, John SL, Nibert, Max L, ...
Abstract Background The reovirus M1 genome segment encodes the μ2 protein, a structurally minor component of the viral core, which has been identified as a transcriptase cofactor, nucleoside and RNA...
Jané-Valbuena, Judit, Nibert, Max L., Spencer, Stephan M., Walker, Stephen B., Baker, Timothy S., Chen, Ya, ...
Structure-function studies with mammalian reoviruses have been limited by the lack of a reverse-genetic system for engineering mutations into the viral genome. To circumvent this limitation in a...
In Vitro Recoating of Reovirus Cores with Baculovirus-Expressed Outer-Capsid Proteins μ1 and ς3
Chandran, Kartik, Walker, Stephen B., Chen, Ya, Contreras, Carlo M., Schiff, Leslie A., Baker, Timothy S., ...
Reovirus outer-capsid proteins μ1, ς3, and ς1 are thought to be assembled onto nascent core-like particles within infected cells, leading to the production of progeny virions. Consistent with this...
Chandran, Kartik, Nibert, Max L.
Mammalian reovirus virions undergo partial disassembly of the outer capsid upon exposure to proteases in vitro, producing infectious subvirion particles (ISVPs) that lack protein ς3 and contain...
Chappell, James D., Barton, Erik S., Smith, Trent H., Baer, Geoffrey S., Duong, David T., Nibert, Max L., ...
A requisite step in reovirus infection of the murine intestine is proteolysis of outer-capsid proteins to yield infectious subvirion particles (ISVPs). When converted to ISVPs by intestinal...
Broering, Teresa J., McCutcheon, Aimee M., Centonze, Victoria E., Nibert, Max L.
Previous studies provided evidence that nonstructural protein μNS of mammalian reoviruses is present in particle assembly intermediates isolated from infected cells. Morgan and Zweerink (Virology...
Gillian, Anne Lynn, Schmechel, Stephen C., Livny, Jonathan, Schiff, Leslie A., Nibert, Max L.
Reovirus nonstructural protein ςNS interacts with reovirus plus-strand RNAs in infected cells, but little is known about the nature of those interactions or their roles in viral replication. In this...
Chandran, Kartik, Zhang, Xing, Olson, Norman H., Walker, Stephen B., Chappell, James D., Dermody, Terence S., ...
Mammalian reoviruses, prototype members of the Reoviridae family of nonenveloped double-stranded RNA viruses, use at least three proteins—ς1, μ1, and ς3—to enter host cells. ς1, a major...
Middleton, Jason K., Severson, Tonya F., Chandran, Kartik, Gillian, Anne Lynn, Yin, John, Nibert, Max L.
Kinetic analyses of infectivity loss during thermal inactivation of reovirus particles revealed substantial differences between virions and infectious subvirion particles (ISVPs), as well as between...
Jané-Valbuena, Judit, Breun, Laura A., Schiff, Leslie A., Nibert, Max L.
Entry of mammalian reovirus virions into target cells requires proteolytic processing of surface protein σ3. In the virion, σ3 mostly covers the membrane-penetration protein μ1, appearing to keep...
Chandran, Kartik, Farsetta, Diane L., Nibert, Max L.
The mechanisms employed by nonenveloped animal viruses to penetrate the membranes of their host cells remain enigmatic. Membrane penetration by the nonenveloped mammalian reoviruses is believed to...
Kim, Jonghwa, Zhang, Xing, Centonze, Victoria E., Bowman, Valorie D., Noble, Simon, Baker, Timothy S., ...
The reovirus core particle is a molecular machine that mediates synthesis, capping, and export of the viral plus strand RNA transcripts. Its assembly and structure-function relationships remain to be...
Structure of the reovirus outer capsid and dsRNA-binding protein σ3 at 1.8 Å resolution
Olland, Andrea M., Jané-Valbuena, Judit, Schiff, Leslie A., Nibert, Max L., Harrison, Stephen C.
The crystallographically determined structure of the reovirus outer capsid protein σ3 reveals a two-lobed structure organized around a long central helix. The smaller of the two lobes includes a...
Reovirus σNS Protein Localizes to Inclusions through an Association Requiring the μNS Amino Terminus
Miller, Cathy L., Broering, Teresa J., Parker, John S. L., Arnold, Michelle M., Nibert, Max L.
Cells infected with mammalian reoviruses contain phase-dense inclusions, called viral factories, in which viral replication and assembly are thought to occur. The major reovirus nonstructural protein...
Odegard, Amy L., Chandran, Kartik, Liemann, Susanne, Harrison, Stephen C., Nibert, Max L.
We examined how a particular type of intermolecular disulfide (ds) bond is formed in the capsid of a cytoplasmically replicating nonenveloped animal virus despite the normally reducing environment...
Helander, Anna, Silvey, Katherine J., Mantis, Nicholas J., Hutchings, Amy B., Chandran, Kartik, Lucas, William T., ...
Type 1 reoviruses invade the intestinal mucosa of mice by adhering selectively to M cells in the follicle-associated epithelium and then exploiting M cell transport activity. The purpose of this...
Chandran, Kartik, Parker, John S. L., Ehrlich, Marcelo, Kirchhausen, Tomas, Nibert, Max L.
Cell entry by reoviruses requires a large, transcriptionally active subvirion particle to gain access to the cytoplasm. The features of this particle have been the subject of debate, but three...
Hutchings, Amy B., Helander, Anna, Silvey, Katherine J., Chandran, Kartik, Lucas, William T., Nibert, Max L., ...
Reovirus type 1 Lang (T1L) adheres to M cells in the follicle-associated epithelium of mouse intestine and exploits the transport activity of M cells to enter and infect the Peyer's patch mucosa....
Broering, Teresa J., Kim, Jonghwa, Miller, Cathy L., Piggott, Caroline D. S., Dinoso, Jason B., Nibert, Max L., ...
Mammalian reoviruses are thought to assemble and replicate within cytoplasmic, nonmembranous structures called viral factories. The viral nonstructural protein μNS forms factory-like globular...
Odegard, Amy L., Chandran, Kartik, Zhang, Xing, Parker, John S. L., Baker, Timothy S., Nibert, Max L.
Several nonenveloped animal viruses possess an autolytic capsid protein that is cleaved as a maturation step during assembly to yield infectious virions. The 76-kDa major outer capsid protein μ1 of...
Miller, Cathy L., Parker, John S. L., Dinoso, Jason B., Piggott, Caroline D. S., Perron, Michel J., Nibert, Max L.
Reovirus replication and assembly are thought to occur within cytoplasmic inclusion bodies, which we call viral factories. A strain-dependent difference in the morphology of these structures reflects...
Helander, Anna, Miller, Cathy L., Myers, Kimberly S., Neutra, Marian R., Nibert, Max L.
Nonfusogenic mammalian orthoreovirus (reovirus) is an enteric pathogen of mice and a useful model for studies of how an enteric virus crosses the mucosal barrier of its host and is subject to control...
Comparisons of the M1 genome segments and encoded μ2 proteins of different reovirus isolates
Yin, Peng, Keirstead, Natalie D, Broering, Teresa J, Arnold, Michelle M, Parker, John SL, Nibert, Max L, ...
Broering, Teresa J., Arnold, Michelle M., Miller, Cathy L., Hurt, Jessica A., Joyce, Patricia L., Nibert, Max L.
Mammalian orthoreoviruses are believed to replicate in distinctive, cytoplasmic inclusion bodies, commonly called viral factories or viroplasms. The viral nonstructural protein μNS has been...
Parker, John S. L., Broering, Teresa J., Kim, Jonghwa, Higgins, Darren E., Nibert, Max L.
Cells infected with mammalian reoviruses often contain large perinuclear inclusion bodies, or “factories,” where viral replication and assembly are thought to occur. Here, we report a viral...
Broering, Teresa J., Parker, John S. L., Joyce, Patricia L., Kim, Jonghwa, Nibert, Max L.
Cells infected with mammalian orthoreoviruses contain large cytoplasmic phase-dense inclusions believed to be the sites of viral replication and assembly, but the morphogenesis, structure, and...
Jané-Valbuena, Judit, Nibert, Max L., Spencer, Stephan M., Walker, Stephen B., Baker, Timothy S., Chen, Ya, ...
Structure-function studies with mammalian reoviruses have been limited by the lack of a reverse-genetic system for engineering mutations into the viral genome. To circumvent this limitation in a...
In Vitro Recoating of Reovirus Cores with Baculovirus-Expressed Outer-Capsid Proteins μ1 and ς3
Chandran, Kartik, Walker, Stephen B., Chen, Ya, Contreras, Carlo M., Schiff, Leslie A., Baker, Timothy S., ...
Reovirus outer-capsid proteins μ1, ς3, and ς1 are thought to be assembled onto nascent core-like particles within infected cells, leading to the production of progeny virions. Consistent with this...
Chandran, Kartik, Nibert, Max L.
Mammalian reovirus virions undergo partial disassembly of the outer capsid upon exposure to proteases in vitro, producing infectious subvirion particles (ISVPs) that lack protein ς3 and contain...
Chappell, James D., Barton, Erik S., Smith, Trent H., Baer, Geoffrey S., Duong, David T., Nibert, Max L., ...
A requisite step in reovirus infection of the murine intestine is proteolysis of outer-capsid proteins to yield infectious subvirion particles (ISVPs). When converted to ISVPs by intestinal...
Broering, Teresa J., McCutcheon, Aimee M., Centonze, Victoria E., Nibert, Max L.
Previous studies provided evidence that nonstructural protein μNS of mammalian reoviruses is present in particle assembly intermediates isolated from infected cells. Morgan and Zweerink (Virology...
Gillian, Anne Lynn, Schmechel, Stephen C., Livny, Jonathan, Schiff, Leslie A., Nibert, Max L.
Reovirus nonstructural protein ςNS interacts with reovirus plus-strand RNAs in infected cells, but little is known about the nature of those interactions or their roles in viral replication. In this...
Chandran, Kartik, Zhang, Xing, Olson, Norman H., Walker, Stephen B., Chappell, James D., Dermody, Terence S., ...
Mammalian reoviruses, prototype members of the Reoviridae family of nonenveloped double-stranded RNA viruses, use at least three proteins—ς1, μ1, and ς3—to enter host cells. ς1, a major...
Middleton, Jason K., Severson, Tonya F., Chandran, Kartik, Gillian, Anne Lynn, Yin, John, Nibert, Max L.
Kinetic analyses of infectivity loss during thermal inactivation of reovirus particles revealed substantial differences between virions and infectious subvirion particles (ISVPs), as well as between...
Jané-Valbuena, Judit, Breun, Laura A., Schiff, Leslie A., Nibert, Max L.
Entry of mammalian reovirus virions into target cells requires proteolytic processing of surface protein σ3. In the virion, σ3 mostly covers the membrane-penetration protein μ1, appearing to keep...
Chandran, Kartik, Farsetta, Diane L., Nibert, Max L.
The mechanisms employed by nonenveloped animal viruses to penetrate the membranes of their host cells remain enigmatic. Membrane penetration by the nonenveloped mammalian reoviruses is believed to...
Kim, Jonghwa, Zhang, Xing, Centonze, Victoria E., Bowman, Valorie D., Noble, Simon, Baker, Timothy S., ...
The reovirus core particle is a molecular machine that mediates synthesis, capping, and export of the viral plus strand RNA transcripts. Its assembly and structure-function relationships remain to be...
Structure of the reovirus outer capsid and dsRNA-binding protein σ3 at 1.8 Å resolution
Olland, Andrea M., Jané-Valbuena, Judit, Schiff, Leslie A., Nibert, Max L., Harrison, Stephen C.
The crystallographically determined structure of the reovirus outer capsid protein σ3 reveals a two-lobed structure organized around a long central helix. The smaller of the two lobes includes a...
Reovirus σNS Protein Localizes to Inclusions through an Association Requiring the μNS Amino Terminus
Miller, Cathy L., Broering, Teresa J., Parker, John S. L., Arnold, Michelle M., Nibert, Max L.
Cells infected with mammalian reoviruses contain phase-dense inclusions, called viral factories, in which viral replication and assembly are thought to occur. The major reovirus nonstructural protein...
Odegard, Amy L., Chandran, Kartik, Liemann, Susanne, Harrison, Stephen C., Nibert, Max L.
We examined how a particular type of intermolecular disulfide (ds) bond is formed in the capsid of a cytoplasmically replicating nonenveloped animal virus despite the normally reducing environment...
Parker, John S. L., Broering, Teresa J., Kim, Jonghwa, Higgins, Darren E., Nibert, Max L.
Cells infected with mammalian reoviruses often contain large perinuclear inclusion bodies, or “factories,” where viral replication and assembly are thought to occur. Here, we report a viral...
Broering, Teresa J., Parker, John S. L., Joyce, Patricia L., Kim, Jonghwa, Nibert, Max L.
Cells infected with mammalian orthoreoviruses contain large cytoplasmic phase-dense inclusions believed to be the sites of viral replication and assembly, but the morphogenesis, structure, and...
Helander, Anna, Silvey, Katherine J., Mantis, Nicholas J., Hutchings, Amy B., Chandran, Kartik, Lucas, William T., ...
Type 1 reoviruses invade the intestinal mucosa of mice by adhering selectively to M cells in the follicle-associated epithelium and then exploiting M cell transport activity. The purpose of this...
Chandran, Kartik, Parker, John S. L., Ehrlich, Marcelo, Kirchhausen, Tomas, Nibert, Max L.
Cell entry by reoviruses requires a large, transcriptionally active subvirion particle to gain access to the cytoplasm. The features of this particle have been the subject of debate, but three...
Hutchings, Amy B., Helander, Anna, Silvey, Katherine J., Chandran, Kartik, Lucas, William T., Nibert, Max L., ...
Reovirus type 1 Lang (T1L) adheres to M cells in the follicle-associated epithelium of mouse intestine and exploits the transport activity of M cells to enter and infect the Peyer's patch mucosa....
Broering, Teresa J., Kim, Jonghwa, Miller, Cathy L., Piggott, Caroline D. S., Dinoso, Jason B., Nibert, Max L., ...
Mammalian reoviruses are thought to assemble and replicate within cytoplasmic, nonmembranous structures called viral factories. The viral nonstructural protein μNS forms factory-like globular...
Odegard, Amy L., Chandran, Kartik, Zhang, Xing, Parker, John S. L., Baker, Timothy S., Nibert, Max L.
Several nonenveloped animal viruses possess an autolytic capsid protein that is cleaved as a maturation step during assembly to yield infectious virions. The 76-kDa major outer capsid protein μ1 of...
Miller, Cathy L., Parker, John S. L., Dinoso, Jason B., Piggott, Caroline D. S., Perron, Michel J., Nibert, Max L.
Reovirus replication and assembly are thought to occur within cytoplasmic inclusion bodies, which we call viral factories. A strain-dependent difference in the morphology of these structures reflects...
Helander, Anna, Miller, Cathy L., Myers, Kimberly S., Neutra, Marian R., Nibert, Max L.
Nonfusogenic mammalian orthoreovirus (reovirus) is an enteric pathogen of mice and a useful model for studies of how an enteric virus crosses the mucosal barrier of its host and is subject to control...
Comparisons of the M1 genome segments and encoded μ2 proteins of different reovirus isolates
Yin, Peng, Keirstead, Natalie D, Broering, Teresa J, Arnold, Michelle M, Parker, John SL, Nibert, Max L, ...
Broering, Teresa J., Arnold, Michelle M., Miller, Cathy L., Hurt, Jessica A., Joyce, Patricia L., Nibert, Max L.
Mammalian orthoreoviruses are believed to replicate in distinctive, cytoplasmic inclusion bodies, commonly called viral factories or viroplasms. The viral nonstructural protein μNS has been...
Coffey, Caroline M., Sheh, Alexander, Kim, Irene S., Chandran, Kartik, Nibert, Max L., Parker, John S. L.
The mechanisms by which reoviruses induce apoptosis have not been fully elucidated. Earlier studies identified the mammalian reovirus S1 and M2 genes as determinants of apoptosis induction. However,...
Reovirus μ1 Structural Rearrangements That Mediate Membrane Penetration▿
Zhang, Lan, Chandran, Kartik, Nibert, Max L., Harrison, Stephen C.
Membrane penetration by nonenveloped reoviruses is mediated by the outer-capsid protein, μ1 (76 kDa). Previous evidence has suggested that an autolytic cleavage in μ1 allows the release of its...
Agosto, Melina A., Ivanovic, Tijana, Nibert, Max L.
During cell entry, reovirus particles with a diameter of 70–80 nm must penetrate the cellular membrane to access the cytoplasm. The mechanism of penetration, without benefit of membrane fusion, is...
Murray, Kenneth E., Nibert, Max L.
Millimolar concentrations of guanidine hydrochloride (GuHCl) are known to inhibit the replication of many plant and animal viruses having positive-sense RNA genomes. For example, GuHCl reversibly...
Agosto, Melina A., Middleton, Jason K., Freimont, Elaine C., Yin, John, Nibert, Max L.
Heat-resistant mutants selected from infectious subvirion particles of mammalian reoviruses have determinative mutations in the major outer-capsid protein μ1. Here we report the isolation and...
Human Papillomavirus Type 16 E7 Oncoprotein Associates with the Centrosomal Component γ-Tubulin▿
Nguyen, Christine L., Eichwald, Catherine, Nibert, Max L., Münger, Karl
Expression of a high-risk human papillomavirus (HPV) E7 oncoprotein is sufficient to induce aberrant centrosome duplication in primary human cells. The resulting centrosome-associated mitotic...
Agosto, Melina A., Myers, Kimberly S., Ivanovic, Tijana, Nibert, Max L.
Membrane penetration by reovirus is associated with conversion of a metastable intermediate, the ISVP, to a further-disassembled particle, the ISVP*. Factors that promote this conversion in cells are...
Peptides released from reovirus outer capsid form membrane pores that recruit virus particles
Ivanovic, Tijana, Agosto, Melina A, Zhang, Lan, Chandran, Kartik, Harrison, Stephen C, Nibert, Max L
Nonenveloped animal viruses must disrupt or perforate a cell membrane during entry. Recent work with reovirus has shown formation of size-selective pores in RBC membranes in concert with structural...
Tang, Jinghua, Ochoa, Wendy F., Sinkovits, Robert S., Poulos, Bonnie T., Ghabrial, Said A., Lightner, Donald V., ...
Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an...
Atomic structure reveals the unique capsid organization of a dsRNA virus
Pan, Junhua, Dong, Liping, Lin, Li, Ochoa, Wendy F., Sinkovits, Robert S., Havens, Wendy M., ...
For most dsRNA viruses, the genome-enclosing capsid comprises 120 copies of a single capsid protein (CP) organized into 60 icosahedrally equivalent dimers, generally identified as 2...