Melissa A. Ashlock

Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences

Henning, Karla A., Novotny, Elizabeth A., Compton, Sheila T., Guan, Xin-Yuan, Liu, Pu P., Ashlock, Melissa A.

A human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was selected based on its size from among several YACs identified by screening a randomly...

Humanizing the yeast telomerase template

Henning, Karla A., Moskowitz, Naomi, Ashlock, Melissa A., Liu, Pu P.

Saccharomyces cerevisiae contains an irregular telomere sequence (TG1–3)n, which differs from the regular repeat (TTAGGG)n found at the telomeres of higher organisms including humans. We have...

Isolation of Human Transcripts Expressed in Hamster Cells from YACs by cDNA Representational Difference Analysis

Gu, Jessie, Guan, Xin-Yuan, Ashlock, Melissa A.

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework...

Human artificial chromosomes generated by modification of a yeast artificial chromosome containing both human alpha satellite and single-copy DNA sequences

Henning, Karla A., Novotny, Elizabeth A., Compton, Sheila T., Guan, Xin-Yuan, Liu, Pu P., Ashlock, Melissa A.

A human artificial chromosome (HAC) vector was constructed from a 1-Mb yeast artificial chromosome (YAC) that was selected based on its size from among several YACs identified by screening a randomly...

Humanizing the yeast telomerase template

Henning, Karla A., Moskowitz, Naomi, Ashlock, Melissa A., Liu, Pu P.

Saccharomyces cerevisiae contains an irregular telomere sequence (TG1–3)n, which differs from the regular repeat (TTAGGG)n found at the telomeres of higher organisms including humans. We have...

Isolation of Human Transcripts Expressed in Hamster Cells from YACs by cDNA Representational Difference Analysis

Gu, Jessie, Guan, Xin-Yuan, Ashlock, Melissa A.

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework...