Michèle Caizergues-Ferrer

Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p (2001)

Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves

Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p (2001)

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we...

The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase (1998)

Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David

Cloning of cDNA encoding a 100 kDa nudeolar protein (nucleoline) of Chinese hamster ovary cells (1985)

Lapeyre, Bruno, Caizergues-Ferrer, Michèle, Bouche, Gérard, Amalric, François

Nucleoline (100 kDa) is the major nucleolar protein in exponentially growing cells that behaves like a nucleolar organizer protein and plays a key role in rDNA transcription and prerRNA processing....

Interrelations between the maturation of a 100 kDa nucleolar protein and pre rRNA synthesis in CHO cells (1984)

Bouche, Gérard, Caizergues-Ferrer, Michèle, Bugler, Béatrix, Amalric, François

The synthesis of preribosomal RNA is inhibited “in vivo” and “in vitro” by the protease inhibitor leupeptin. “In vivo” leupeptin decreases by 74 % the incorporation of labeled uridine...

The Role of the Schizosaccharomyces pombe gar2 Protein in Nucleolar Structure and Function Depends on the Concerted Action of its Highly Charged N Terminus and its RNA-binding Domains

Sicard, Hélène, Faubladier, Marlène, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Gas, Nicole, Caizergues-Ferrer, Michèle

Nonribosomal nucleolar protein gar2 is required for 18S rRNA and 40S ribosomal subunit production in Schizosaccharomyces pombe. We have investigated the consequences of the absence of each structural...

Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p

Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves

Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we show that...

Seven Novel Methylation Guide Small Nucleolar RNAs Are Processed from a Common Polycistronic Transcript by Rat1p and RNase III in Yeast

Qu, Liang-Hu, Henras, Anthony, Lu, Yong-Jun, Zhou, Hui, Zhou, Wei-xin, Zhu, Yuan-Qi, ...

Through a computer search of the genome of the yeast Saccharomyces cerevisiae, the coding sequences of seven different box C/D antisense small nucleolar RNAs (snoRNAs) with the structural hallmarks...

Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs†

Dez, Christophe, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Henry, Yves

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. The ways in which these particles are assembled and correctly localized...

Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein

Vanrobays, Emmanuel, Gleizes, Pierre-Emmanuel, Bousquet-Antonelli, Cécile, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Gélugne, Jean-Paul

Numerous non-ribosomal trans-acting factors involved in pre-ribosomal RNA processing have been characterized, but none of them is specifically required for the last cytoplasmic steps of 18S rRNA...

The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase

Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David

Many or all of the sites of pseudouridine (Ψ) formation in eukaryotic rRNA are selected by site-specific base-pairing with members of the box H + ACA class of small nucleolar RNAs (snoRNAs)....

Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification†

Dez, Christophe, Froment, Carine, Noaillac-Depeyre, Jacqueline, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, Henry, Yves

We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S...

The Splicing ATPase Prp43p Is a Component of Multiple Preribosomal Particles

Lebaron, Simon, Froment, Carine, Fromont-Racine, Micheline, Rain, Jean-Christophe, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, ...

Prp43p is a putative helicase of the DEAH family which is required for the release of the lariat intron from the spliceosome. Prp43p could also play a role in ribosome synthesis, since it accumulates...

The Role of the Schizosaccharomyces pombe gar2 Protein in Nucleolar Structure and Function Depends on the Concerted Action of its Highly Charged N Terminus and its RNA-binding Domains

Sicard, Hélène, Faubladier, Marlène, Noaillac-Depeyre, Jacqueline, Léger-Silvestre, Isabelle, Gas, Nicole, Caizergues-Ferrer, Michèle

Nonribosomal nucleolar protein gar2 is required for 18S rRNA and 40S ribosomal subunit production in Schizosaccharomyces pombe. We have investigated the consequences of the absence of each structural...

Stable expression in yeast of the mature form of human telomerase RNA depends on its association with the box H/ACA small nucleolar RNP proteins Cbf5p, Nhp2p and Nop10p

Dez, Christophe, Henras, Anthony, Faucon, Bruno, Lafontaine, Denis L. J., Caizergues-Ferrer, Michèle, Henry, Yves

Telomerase is a ribonucleoprotein (RNP) particle required for the replication of telomeres. The RNA component, termed hTR, of human telomerase contains a domain structurally and functionally related...

Accumulation of H/ACA snoRNPs depends on the integrity of the conserved central domain of the RNA-binding protein Nhp2p

Henras, Anthony, Dez, Christophe, Noaillac-Depeyre, Jacqueline, Henry, Yves, Caizergues-Ferrer, Michèle

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. How box H/ACA snoRNPs are assembled remains unknown. Here we show that...

Seven Novel Methylation Guide Small Nucleolar RNAs Are Processed from a Common Polycistronic Transcript by Rat1p and RNase III in Yeast

Qu, Liang-Hu, Henras, Anthony, Lu, Yong-Jun, Zhou, Hui, Zhou, Wei-xin, Zhu, Yuan-Qi, ...

Through a computer search of the genome of the yeast Saccharomyces cerevisiae, the coding sequences of seven different box C/D antisense small nucleolar RNAs (snoRNAs) with the structural hallmarks...

Naf1p, an Essential Nucleoplasmic Factor Specifically Required for Accumulation of Box H/ACA Small Nucleolar RNPs†

Dez, Christophe, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Henry, Yves

Box H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) play key roles in the synthesis of eukaryotic ribosomes. The ways in which these particles are assembled and correctly localized...

Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic protein

Vanrobays, Emmanuel, Gleizes, Pierre-Emmanuel, Bousquet-Antonelli, Cécile, Noaillac-Depeyre, Jacqueline, Caizergues-Ferrer, Michèle, Gélugne, Jean-Paul

Numerous non-ribosomal trans-acting factors involved in pre-ribosomal RNA processing have been characterized, but none of them is specifically required for the last cytoplasmic steps of 18S rRNA...

The box H+ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase

Lafontaine, Denis L.J., Bousquet-Antonelli, Cécile, Henry, Yves, Caizergues-Ferrer, Michèle, Tollervey, David

Many or all of the sites of pseudouridine (Ψ) formation in eukaryotic rRNA are selected by site-specific base-pairing with members of the box H + ACA class of small nucleolar RNAs (snoRNAs)....

Npa1p, a Component of Very Early Pre-60S Ribosomal Particles, Associates with a Subset of Small Nucleolar RNPs Required for Peptidyl Transferase Center Modification†

Dez, Christophe, Froment, Carine, Noaillac-Depeyre, Jacqueline, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, Henry, Yves

We have identified a novel essential nucleolar factor required for the synthesis of 5.8S and 25S rRNAs termed Npa1p. In the absence of Npa1p, the pre-rRNA processing pathway leading to 5.8S and 25S...

The Splicing ATPase Prp43p Is a Component of Multiple Preribosomal Particles

Lebaron, Simon, Froment, Carine, Fromont-Racine, Micheline, Rain, Jean-Christophe, Monsarrat, Bernard, Caizergues-Ferrer, Michèle, ...

Prp43p is a putative helicase of the DEAH family which is required for the release of the lariat intron from the spliceosome. Prp43p could also play a role in ribosome synthesis, since it accumulates...

Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) Reveals a Low-Molecular-Mass Complex Containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p Involved in Early Steps of 60S Ribosomal Subunit Biogenesis▿ †

Rosado, Iván V., Dez, Christophe, Lebaron, Simon, Caizergues-Ferrer, Michèle, Henry, Yves, De La Cruz, Jesús

We report the characterization of the yeast Npa2p (Urb2p) protein, which is essential for 60S ribosomal subunit biogenesis. We identified this protein in a synthetic lethal screening with the rsa3...