Michèle Mock

Primary Involvement of Pharynx and Peyer's Patch in Inhalational and Intestinal Anthrax (2007)

Ian J. Glomski, Alejandro Piris-Gimenez, Michel Huerre, Michèle Mock, Pierre L. Goossens

Bacillus anthracis causes three forms of anthrax: inhalational, gastrointestinal, and cutaneous. Anthrax is characterized by both toxemia, which is caused by secretion of immunomodulating toxins...

Screening inhibitors of anthrax lethal factor (2002)

Tonello, Fiorella, Seveso, Michela, Marin, Oriano, Mock, Michèle, Montecucco, Cesare

The disease anthrax is caused by lethal factor, an enzyme component of the toxin produced by the spore-forming bacterium Bacillus anthracis. Here we describe substrate molecules for this factor that...

Nucleotide sequence for the catalytic domain of colicin E3 and its immunity protein. Evidence for a third gene overlapping colicin (1983)

Mock, Michèle, Miyada, C. Garrett, Gunsalus, Robert P.

We have determined the nucleotide sequence of a segment of Co1E3 DNA coding for the carboxyl–terminal, catalytic peptide of colicin E3 and for the immunity protein. The end of the colicin E3 gene...

Cell Surface-Exposed Tetanus Toxin Fragment C Produced by Recombinant Bacillus anthracis Protects against Tetanus Toxin

Mesnage, Stéphane, Weber-Levy, Martine, Haustant, Michel, Mock, Michèle, Fouet, Agnès

Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B....

Protective Antigen-Mediated Antibody Response against a Heterologous Protein Produced In Vivo by Bacillus anthracis

Brossier, Fabien, Weber-Levy, Martine, Mock, Michèle, Sirard, Jean-Claude

Bacillus anthracis secretes a lethal toxin composed of two proteins, the lethal factor (LF) and the protective antigen (PA), which interact within the host or in vitro at the surfaces of eukaryotic...

Molecular Characterization of Bacillus Strains Involved in Outbreaks of Anthrax in France in 1997

Patra, Guy, Vaissaire, Josée, Weber-Levy, Martine, Le Doujet, Claudine, Mock, Michèle

Outbreaks of anthrax zoonose occurred in two regions of France in 1997. Ninety-four animals died, and there were three nonfatal cases in humans. The diagnosis of anthrax was rapidly confirmed by...

The Capsule and S-Layer: Two Independent and Yet Compatible Macromolecular Structures in Bacillus anthracis

Mesnage, Stéphane, Tosi-Couture, Evelyne, Gounon, Pierre, Mock, Michèle, Fouet, Agnès

Bacillus anthracis, the etiological agent of anthrax, is a gram-positive spore-forming bacterium. Fully virulent bacilli are toxinogenic and capsulated. Two abundant surface proteins, including the...

Anthrax Spores Make an Essential Contribution to Vaccine Efficacy

Brossier, Fabien, Levy, Martine, Mock, Michèle

Anthrax is caused by Bacillus anthracis, a gram-positive spore-forming bacterium. Septicemia and toxemia rapidly lead to death in infected mammal hosts. Currently used acellular vaccines against...

Polymorphism in the Collagen-Like Region of the Bacillus anthracis BclA Protein Leads to Variation in Exosporium Filament Length

Sylvestre, Patricia, Couture-Tosi, Evelyne, Mock, Michèle

We recently identified a Bacillus anthracis glycoprotein which is a structural constituent of the exosporium filaments (P. Sylvestre, E. Couture-Tosi, and M. Mock, Mol. Microbiol. 45:169-178, 2002)....

Diversity among French Bacillus anthracis Isolates

Fouet, Agnès, Smith, Kimothy L., Keys, Chris, Vaissaire, Josée, Le Doujet, Claudine, Lévy, Martine, ...

While outbreaks of animal anthrax zoonoses still regularly occur in France, little is known about the epidemiology links between them. We have used the eight-locus multilocus variable-number tandem...

The BtuB Group Col Plasmids and Homology Between the Colicins They Encode

Mock, Michèle, Pugsley, Anthony P.

Colicins A, E1, E2, E3, E4, E5, E6, and E7 exhibited reduced activity against BtuB mutants of Escherichia coli K-12 and also against wild-type cells in the presence of vitamin B12. Plasmids encoding...

Action of Lysozyme on Penicillin-Induced Filaments of Proteus vulgaris

Fleck, Jacqueline, Martin, Jean-Pierre, Mock, Michèle

Penicillin-induced filaments of Proteus vulgaris P 18 had a seemingly unaltered cell envelope. Lysozyme, however, reduced the wall part of these filaments into a three-layered structure and caused...

Bacterial SLH domain proteins are non-covalently anchored to the cell surface via a conserved mechanism involving wall polysaccharide pyruvylation

Mesnage, Stéphane, Fontaine, Thierry, Mignot, Tâm, Delepierre, Muriel, Mock, Michèle, Fouet, Agnès

Several bacterial proteins are non-covalently anchored to the cell surface via an S-layer homology (SLH) domain. Previous studies have suggested that this cell surface display mechanism involves a...

Distinct Mutations in PlcR Explain Why Some Strains of the Bacillus cereus Group Are Nonhemolytic

Slamti, Leyla, Perchat, Stéphane, Gominet, Myriam, Vilas-Bôas, Gislayne, Fouet, Agnès, Mock, Michèle, ...

Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis are closely related species belonging to the Bacillus cereus group. B. thuringiensis and B. cereus generally produce extracellular...

Functional Analysis of Bacillus anthracis Protective Antigen by Using Neutralizing Monoclonal Antibodies†

Brossier, Fabien, Lévy, Martine, Landier, Annie, Lafaye, Pierre, Mock, Michèle

Protective antigen (PA) is central to the action of the lethal and edema toxins produced by Bacillus anthracis. It is the common cell-binding component, mediating the translocation of the enzymatic...

Contribution of ExsFA and ExsFB Proteins to the Localization of BclA on the Spore Surface and to the Stability of the Bacillus anthracis Exosporium

Sylvestre, Patricia, Couture-Tosi, Evelyne, Mock, Michèle

Spores of Bacillus anthracis, the etiological agent of anthrax, and the closely related species Bacillus cereus and Bacillus thuringiensis, possess an exosporium, which is the outermost structure...

Identification of the Bacillus anthracis γ Phage Receptor

Davison, Sophie, Couture-Tosi, Evelyne, Candela, Thomas, Mock, Michèle, Fouet, Agnès

Bacillus anthracis, a gram-positive, spore-forming bacterium, is the etiological agent of anthrax. It belongs to the Bacillus cereus group, which also contains Bacillus cereus and Bacillus...

CapE, a 47-Amino-Acid Peptide, Is Necessary for Bacillus anthracis Polyglutamate Capsule Synthesis

Candela, Thomas, Mock, Michèle, Fouet, Agnès

Polyglutamate is found in various bacteria, but displays different functions depending on the species and their environment. Here, we describe a minimal polyglutamate synthesis system in Bacillus...

Potent inhibitors of anthrax lethal factor from green tea

Dell'Aica, Isabella, Donà, Massimo, Tonello, Fiorella, Piris, Alejandro, Mock, Michèle, Montecucco, Cesare, ...

The anthrax lethal factor (LF) has a major role in the development of anthrax. LF is delivered by the protective antigen (PA) inside the cell, where it exerts its metalloprotease activity on the...

Cell Surface-Exposed Tetanus Toxin Fragment C Produced by Recombinant Bacillus anthracis Protects against Tetanus Toxin

Mesnage, Stéphane, Weber-Levy, Martine, Haustant, Michel, Mock, Michèle, Fouet, Agnès

Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B....

Protective Antigen-Mediated Antibody Response against a Heterologous Protein Produced In Vivo by Bacillus anthracis

Brossier, Fabien, Weber-Levy, Martine, Mock, Michèle, Sirard, Jean-Claude

Bacillus anthracis secretes a lethal toxin composed of two proteins, the lethal factor (LF) and the protective antigen (PA), which interact within the host or in vitro at the surfaces of eukaryotic...

Molecular Characterization of Bacillus Strains Involved in Outbreaks of Anthrax in France in 1997

Patra, Guy, Vaissaire, Josée, Weber-Levy, Martine, Le Doujet, Claudine, Mock, Michèle

Outbreaks of anthrax zoonose occurred in two regions of France in 1997. Ninety-four animals died, and there were three nonfatal cases in humans. The diagnosis of anthrax was rapidly confirmed by...

The Capsule and S-Layer: Two Independent and Yet Compatible Macromolecular Structures in Bacillus anthracis

Mesnage, Stéphane, Tosi-Couture, Evelyne, Gounon, Pierre, Mock, Michèle, Fouet, Agnès

Bacillus anthracis, the etiological agent of anthrax, is a gram-positive spore-forming bacterium. Fully virulent bacilli are toxinogenic and capsulated. Two abundant surface proteins, including the...

Anthrax Spores Make an Essential Contribution to Vaccine Efficacy

Brossier, Fabien, Levy, Martine, Mock, Michèle

Anthrax is caused by Bacillus anthracis, a gram-positive spore-forming bacterium. Septicemia and toxemia rapidly lead to death in infected mammal hosts. Currently used acellular vaccines against...

Polymorphism in the Collagen-Like Region of the Bacillus anthracis BclA Protein Leads to Variation in Exosporium Filament Length

Sylvestre, Patricia, Couture-Tosi, Evelyne, Mock, Michèle

We recently identified a Bacillus anthracis glycoprotein which is a structural constituent of the exosporium filaments (P. Sylvestre, E. Couture-Tosi, and M. Mock, Mol. Microbiol. 45:169-178, 2002)....

Diversity among French Bacillus anthracis Isolates

Fouet, Agnès, Smith, Kimothy L., Keys, Chris, Vaissaire, Josée, Le Doujet, Claudine, Lévy, Martine, ...

While outbreaks of animal anthrax zoonoses still regularly occur in France, little is known about the epidemiology links between them. We have used the eight-locus multilocus variable-number tandem...

The BtuB Group Col Plasmids and Homology Between the Colicins They Encode

Mock, Michèle, Pugsley, Anthony P.

Colicins A, E1, E2, E3, E4, E5, E6, and E7 exhibited reduced activity against BtuB mutants of Escherichia coli K-12 and also against wild-type cells in the presence of vitamin B12. Plasmids encoding...

Action of Lysozyme on Penicillin-Induced Filaments of Proteus vulgaris

Fleck, Jacqueline, Martin, Jean-Pierre, Mock, Michèle

Penicillin-induced filaments of Proteus vulgaris P 18 had a seemingly unaltered cell envelope. Lysozyme, however, reduced the wall part of these filaments into a three-layered structure and caused...

Bacterial SLH domain proteins are non-covalently anchored to the cell surface via a conserved mechanism involving wall polysaccharide pyruvylation

Mesnage, Stéphane, Fontaine, Thierry, Mignot, Tâm, Delepierre, Muriel, Mock, Michèle, Fouet, Agnès

Several bacterial proteins are non-covalently anchored to the cell surface via an S-layer homology (SLH) domain. Previous studies have suggested that this cell surface display mechanism involves a...

Distinct Mutations in PlcR Explain Why Some Strains of the Bacillus cereus Group Are Nonhemolytic

Slamti, Leyla, Perchat, Stéphane, Gominet, Myriam, Vilas-Bôas, Gislayne, Fouet, Agnès, Mock, Michèle, ...

Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis are closely related species belonging to the Bacillus cereus group. B. thuringiensis and B. cereus generally produce extracellular...

Functional Analysis of Bacillus anthracis Protective Antigen by Using Neutralizing Monoclonal Antibodies†

Brossier, Fabien, Lévy, Martine, Landier, Annie, Lafaye, Pierre, Mock, Michèle

Protective antigen (PA) is central to the action of the lethal and edema toxins produced by Bacillus anthracis. It is the common cell-binding component, mediating the translocation of the enzymatic...

Contribution of ExsFA and ExsFB Proteins to the Localization of BclA on the Spore Surface and to the Stability of the Bacillus anthracis Exosporium

Sylvestre, Patricia, Couture-Tosi, Evelyne, Mock, Michèle

Spores of Bacillus anthracis, the etiological agent of anthrax, and the closely related species Bacillus cereus and Bacillus thuringiensis, possess an exosporium, which is the outermost structure...

Identification of the Bacillus anthracis γ Phage Receptor

Davison, Sophie, Couture-Tosi, Evelyne, Candela, Thomas, Mock, Michèle, Fouet, Agnès

Bacillus anthracis, a gram-positive, spore-forming bacterium, is the etiological agent of anthrax. It belongs to the Bacillus cereus group, which also contains Bacillus cereus and Bacillus...

CapE, a 47-Amino-Acid Peptide, Is Necessary for Bacillus anthracis Polyglutamate Capsule Synthesis

Candela, Thomas, Mock, Michèle, Fouet, Agnès

Polyglutamate is found in various bacteria, but displays different functions depending on the species and their environment. Here, we describe a minimal polyglutamate synthesis system in Bacillus...

Potent inhibitors of anthrax lethal factor from green tea

Dell'Aica, Isabella, Donà, Massimo, Tonello, Fiorella, Piris, Alejandro, Mock, Michèle, Montecucco, Cesare, ...

The anthrax lethal factor (LF) has a major role in the development of anthrax. LF is delivered by the protective antigen (PA) inside the cell, where it exerts its metalloprotease activity on the...

Primary Involvement of Pharynx and Peyer's Patch in Inhalational and Intestinal Anthrax

Glomski, Ian J, Piris-Gimenez, Alejandro, Huerre, Michel, Mock, Michèle, Goossens, Pierre L

Bacillus anthracis causes three forms of anthrax: inhalational, gastrointestinal, and cutaneous. Anthrax is characterized by both toxemia, which is caused by secretion of immunomodulating toxins...

Noncapsulated Toxinogenic Bacillus anthracis Presents a Specific Growth and Dissemination Pattern in Naive and Protective Antigen-Immune Mice▿

Glomski, Ian J., Corre, Jean-Philippe, Mock, Michèle, Goossens, Pierre L.

Bacillus anthracis is a spore-forming bacterium that causes anthrax. B. anthracis has three major virulence factors, namely, lethal toxin, edema toxin, and a poly-γ-d-glutamic acid capsule. The...

Efficacy of a Vaccine Based on Protective Antigen and Killed Spores against Experimental Inhalational Anthrax▿ ‡

Gauthier, Yves P., Tournier, Jean-Nicolas, Paucod, Jean-Charles, Corre, Jean-Philippe, Mock, Michèle, Goossens, Pierre L., ...

Protective antigen (PA)-based anthrax vaccines acting on toxins are less effective than live attenuated vaccines, suggesting that additional antigens may contribute to protective immunity. Several...