Michael A. Brehm

TLR agonists prevent the establishment of allogeneic hematopoietic chimerism in mice treated with costimulation blockade (2009)

Miller, David M., Thornley, Thomas B., Pearson, Todd, Kruger, Annie Joseph, Yamazaki, Masahiro, Shultz, Leonard D., ...

Activation of TLR4 by administration of LPS shortens the survival of skin allografts in mice treated with costimulation blockade through a CD8 T cell-dependent, MyD88-dependent, and type I IFN...

TLR agonists abrogate co-stimulation blockade-induced mixed chimerism and transplantation tolerance. (2008)

Miller, David M., Thornley, Thomas B., Pearson, Todd, Yamazaki, Masahiro, Brehm, Michael A., Rossini, Aldo A., ...

We investigated the mechanisms by which Toll-like receptor (TLR) agonists affect the induction of mixed chimerism and skin allograft survival in mice treated with co-stimulation blockade (CB). We...

Idd loci synergize to prolong islet allograft survival induced by costimulation blockade in NOD mice (2008)

Mangada, Julie A., Pearson, Todd, Brehm, Michael A., Wicker, Linda S., Peterson, Laurence B., Shultz, Leonard D., ...

OBJECTIVE: NOD mice model human type 1 diabetes and are used to investigate tolerance induction protocols for islet transplantation in a setting of autoimmunity. However, costimulation blockade-based...

Type 1 IFN mediates cross-talk between innate and adaptive immunity that abrogates transplantation tolerance (2007)

Thornley, Thomas B., Phillips, Nancy E., Beaudette-Zlatanova, Britte C., Markees, Thomas G., Bahl, Kapil, Brehm, Michael A., ...

TLR activation of innate immunity prevents the induction of transplantation tolerance and shortens skin allograft survival in mice treated with costimulation blockade. The mechanism by which TLR...

Different mechanisms control peripheral and central tolerance in hematopoietic chimeric mice (2007)

Yamazaki, Masahiro, Pearson, Todd, Brehm, Michael A., Miller, David M., Mangada, Julie A., Markees, Thomas G., ...

Regulatory T cells (Treg) are important in peripheral tolerance, but their role in establishing and maintaining hematopoietic mixed chimerism and generating central tolerance is unclear. We now show...

Partial versus full allogeneic hemopoietic chimerization is a preferential means to inhibit type 1 diabetes as the latter induces generalized immunosuppression (2006)

Serreze, David V., Osborne, Melissa A., Chen, Yi-Guang, Chapman, Harold D., Pearson, Todd, Brehm, Michael A., ...

In both humans and NOD mice, particular combinations of MHC genes provide the primary risk factor for development of the autoreactive T cell responses causing type 1 diabetes (T1D). Conversely, other...

Protection against vaccinia virus challenge by CD8 memory T cells resolved by molecular mimicry (2006)

Cornberg, Markus, Sheridan, Brian S., Saccoccio, Frances M., Brehm, Michael A., Selin, Liisa K.

Live vaccinia virus (VV) vaccination has been highly successful in eradicating smallpox. However, the mechanisms of immunity involved in mediating this protective effect are still poorly understood,...

Rapid quantification of naive alloreactive T cells by TNF-alpha production and correlation with allograft rejection in mice (2006)

Brehm, Michael A., Mangada, Julie A., Markees, Thomas G., Pearson, Todd, Daniels, Keith A., Thornley, Thomas B., ...

Allograft transplantation requires chronic immunosuppression, but there is no effective strategy to evaluate the long-term maintenance of immunosuppression other than assessment of graft function....

Transgenic expression of the viral FLIP MC159 causes lpr/gld-like lymphoproliferation and autoimmunity (2006)

Woelfel, Melissa A., Bixby, Jacqueline G., Brehm, Michael A., Chan, Francis Ka-Ming

Death receptor-induced programmed cell death (PCD) is crucial for the maintenance of immune homeostasis. However, interference of downstream death receptor signaling by genetic ablation or transgenic...

Memory of mice and men: CD8+ T-cell cross-reactivity and heterologous immunity (2006)

Selin, Liisa K., Brehm, Michael A., Naumov, Yuri N., Cornberg, Markus, Kim, Sung-Kwon, Clute, Shalyn Catherine, ...

The main functions of memory T cells are to provide protection upon re-exposure to a pathogen and to prevent the re-emergence of low-grade persistent pathogens. Memory T cells achieve these functions...

Endoplasmic reticulum aminopeptidase 1 (ERAP1) trims MHC class I-presented peptides in vivo and plays an important role in immunodominance (2006)

York, Ian A., Brehm, Michael A., Zendzian, Sophia, Towne, Charles Fenton, Rock, Kenneth L.

CD8(+) T cells respond to short peptides bound to MHC class I molecules. Although most antigenic proteins contain many sequences that could bind to MHC class I, few of these peptides actually...

Narrowed TCR repertoire and viral escape as a consequence of heterologous immunity (2006)

Cornberg, Markus, Chen, Alex T., Wilkinson, Lee A., Brehm, Michael A., Kim, Sung-Kwon, Calcagno, Claudia, ...

Why some virus-specific CD8 TCR repertoires are diverse and others restricted or "oligoclonal" has been unknown. We show here that oligoclonality and extreme clonal dominance can be a consequence of...

TLR agonists abrogate costimulation blockade-induced prolongation of skin allografts (2006)

Thornley, Thomas B., Brehm, Michael A., Markees, Thomas G., Shultz, Leonard D., Mordes, John P., Welsh, Raymond M., ...

Costimulation blockade protocols are effective in prolonging allograft survival in animal models and are entering clinical trials, but how environmental perturbants affect graft survival remains...

Rapid production of TNF-alpha following TCR engagement of naive CD8 T cells (2005)

Brehm, Michael A., Daniels, Keith A., Welsh, Raymond M.

The acquisition of effector functions by naive CD8 T cells following TCR engagement is thought to occur sequentially with full functionality being gained only after the initiation of division. We...

Rapid conversion of effector mechanisms from NK to T cells during virus-induced lysis of allogeneic implants in vivo (2005)

Brehm, Michael A., Daniels, Keith A., Ortaldo, John R., Welsh, Raymond M.

Viral infections can strongly stimulate both NK cell and allospecific CD8 T cell responses, and these same effector cells can lyse allogeneic cell lines in vitro. However, the impact of viral...

Preapoptotic phenotype of viral epitope-specific CD8 T cells precludes memory development and is an intrinsic property of the epitope (2004)

Wang, Xiaoting Z., Brehm, Michael A., Welsh, Raymond M.

Virus-specific CD8 T cells after clearance of infection reduce their number in lymphoid organs by apoptotic death and by migration into peripheral tissues. During and after infection, many...

Virus-specific CD8 T cells in peripheral tissues are more resistant to apoptosis than those in lymphoid organs (2003)

Wang, Xiaoting Z., Stepp, Susan E., Brehm, Michael A., Chen, Hong D., Selin, Liisa K., Welsh, Raymond M.

CD8 T cells persist at high frequencies in peripheral organs after resolution of an immune response, and their presence in the periphery is important for resistance to secondary challenge. We show...

Direct visualization of cross-reactive effector and memory allo-specific CD8 T cells generated in response to viral infections (2003)

Brehm, Michael A., Markees, Thomas G., Daniels, Keith A., Greiner, Dale L., Rossini, Aldo A., Welsh, Raymond M.

CD8 T cell cross-reactivity between heterologous viruses has been shown to provide protective immunity, induce immunopathology, influence the immunodominance of epitope-specific T cell responses, and...

Dynamics of memory T cell proliferation under conditions of heterologous immunity and bystander stimulation (2002)

Kim, Sung-Kwon, Brehm, Michael A., Welsh, Raymond M., Selin, Liisa K.

By examining adoptively transferred CSFE-labeled lymphocytic choriomeningitis virus (LCMV)-immune donor T cells in Thy-1 congenic hosts inoculated with viruses or with the cytokine inducer poly(I:C),...

Memory CD8+ T cells in heterologous antiviral immunity and immunopathology in the lung (2001)

Chen, Hong D., Fraire, Armando E., Joris, Isabelle, Brehm, Michael A., Welsh, Raymond M., Selin, Liisa K.

A potent role for memory CD8+ T cells in heterologous immunity was shown with a respiratory mucosal model of viral infection. Memory CD8+ T cells generated after lymphocytic choriomeningitis virus...

Attrition of bystander CD8 T cells during virus-induced T-cell and interferon responses (2001)

McNally, James M., Zarozinski, Christopher C., Lin, Meei Y., Brehm, Michael A., Chen, Hong D., Welsh, Raymond M.

Experiments designed to distinguish virus-specific from non-virus-specific T cells showed that bystander T cells underwent apoptosis and substantial attrition in the wake of a strong T-cell response....

Virus-induced abrogation of transplantation tolerance induced by donor-specific transfusion and anti-CD154 antibody (2000)

Welsh, Raymond M., Markees, Thomas G., Woda, Bruce A., Daniels, Keith A., Brehm, Michael A., Mordes, John P., ...

Treatment with a 2-week course of anti-CD154 antibody and a single transfusion of donor leukocytes (a donor-specific transfusion or DST) permits skin allografts to survive for >100 days in...

Immunization with a Single Major Histocompatibility Complex Class I-Restricted Cytotoxic T-Lymphocyte Recognition Epitope of Herpes Simplex Virus Type 2 Confers Protective Immunity

Blaney, Joseph E., Nobusawa, Eri, Brehm, Michael A., Bonneau, Robert H., Mylin, Lawrence M., Fu, Tong-Ming, ...

We have evaluated the potential of conferring protective immunity to herpes simplex virus type 2 (HSV-2) by selectively inducing an HSV-specific CD8+ cytotoxic T-lymphocyte (CTL) response directed...

Virus-Induced Abrogation of Transplantation Tolerance Induced by Donor-Specific Transfusion and Anti-CD154 Antibody

Welsh, Raymond M., Markees, Thomas G., Woda, Bruce A., Daniels, Keith A., Brehm, Michael A., Mordes, John P., ...

Treatment with a 2-week course of anti-CD154 antibody and a single transfusion of donor leukocytes (a donor-specific transfusion or DST) permits skin allografts to survive for >100 days in...

Attrition of Bystander CD8 T Cells during Virus-Induced T-Cell and Interferon Responses

McNally, James M., Zarozinski, Christopher C., Lin, Meei-Yun, Brehm, Michael A., Chen, Hong D., Welsh, Raymond M.

Experiments designed to distinguish virus-specific from non-virus-specific T cells showed that bystander T cells underwent apoptosis and substantial attrition in the wake of a strong T-cell response....

Narrowed TCR repertoire and viral escape as a consequence of heterologous immunity

Cornberg, Markus, Chen, Alex T., Wilkinson, Lee A., Brehm, Michael A., Kim, Sung-Kwon, Calcagno, Claudia, ...

Why some virus-specific CD8 TCR repertoires are diverse and others restricted or “oligoclonal” has been unknown. We show here that oligoclonality and extreme clonal dominance can be a consequence...

Endoplasmic reticulum aminopeptidase 1 (ERAP1) trims MHC class I-presented peptides in vivo and plays an important role in immunodominance

York, Ian A., Brehm, Michael A., Zendzian, Sophia, Towne, Charles F., Rock, Kenneth L.

CD8+ T cells respond to short peptides bound to MHC class I molecules. Although most antigenic proteins contain many sequences that could bind to MHC class I, few of these peptides actually stimulate...

Immunization with a Single Major Histocompatibility Complex Class I-Restricted Cytotoxic T-Lymphocyte Recognition Epitope of Herpes Simplex Virus Type 2 Confers Protective Immunity

Blaney, Joseph E., Nobusawa, Eri, Brehm, Michael A., Bonneau, Robert H., Mylin, Lawrence M., Fu, Tong-Ming, ...

We have evaluated the potential of conferring protective immunity to herpes simplex virus type 2 (HSV-2) by selectively inducing an HSV-specific CD8+ cytotoxic T-lymphocyte (CTL) response directed...

Virus-Induced Abrogation of Transplantation Tolerance Induced by Donor-Specific Transfusion and Anti-CD154 Antibody

Welsh, Raymond M., Markees, Thomas G., Woda, Bruce A., Daniels, Keith A., Brehm, Michael A., Mordes, John P., ...

Treatment with a 2-week course of anti-CD154 antibody and a single transfusion of donor leukocytes (a donor-specific transfusion or DST) permits skin allografts to survive for >100 days in...

Attrition of Bystander CD8 T Cells during Virus-Induced T-Cell and Interferon Responses

McNally, James M., Zarozinski, Christopher C., Lin, Meei-Yun, Brehm, Michael A., Chen, Hong D., Welsh, Raymond M.

Experiments designed to distinguish virus-specific from non-virus-specific T cells showed that bystander T cells underwent apoptosis and substantial attrition in the wake of a strong T-cell response....

Narrowed TCR repertoire and viral escape as a consequence of heterologous immunity

Cornberg, Markus, Chen, Alex T., Wilkinson, Lee A., Brehm, Michael A., Kim, Sung-Kwon, Calcagno, Claudia, ...

Why some virus-specific CD8 TCR repertoires are diverse and others restricted or “oligoclonal” has been unknown. We show here that oligoclonality and extreme clonal dominance can be a consequence...

Endoplasmic reticulum aminopeptidase 1 (ERAP1) trims MHC class I-presented peptides in vivo and plays an important role in immunodominance

York, Ian A., Brehm, Michael A., Zendzian, Sophia, Towne, Charles F., Rock, Kenneth L.

CD8+ T cells respond to short peptides bound to MHC class I molecules. Although most antigenic proteins contain many sequences that could bind to MHC class I, few of these peptides actually stimulate...

Protection against Vaccinia Virus Challenge by CD8 Memory T Cells Resolved by Molecular Mimicry▿

Cornberg, Markus, Sheridan, Brian S., Saccoccio, Frances M., Brehm, Michael A., Selin, Liisa K.

Live vaccinia virus (VV) vaccination has been highly successful in eradicating smallpox. However, the mechanisms of immunity involved in mediating this protective effect are still poorly understood,...

Rapid quantification of naive alloreactive T cells by TNF-α production and correlation with allograft rejection in mice

Brehm, Michael A., Mangada, Julie, Markees, Thomas G., Pearson, Todd, Daniels, Keith A., Thornley, Thomas B., ...

Allograft transplantation requires chronic immunosuppression, but there is no effective strategy to evaluate the long-term maintenance of immunosuppression other than assessment of graft function....

CHOP Mediates Endoplasmic Reticulum Stress-Induced Apoptosis in Gimap5-Deficient T Cells

Pino, Steven C., O'Sullivan-Murphy, Bryan, Lidstone, Erich A., Yang, Chaoxing, Lipson, Kathryn L., Jurczyk, Agata, ...

Gimap5 (GTPase of the immunity-associated protein 5) has been linked to the regulation of T cell survival, and polymorphisms in the human GIMAP5 gene associate with autoimmune disorders. The...